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Quan et al demonstrated that the introduction of DNA into a cell, even without integration, can trigger the oncogenic cGAS-STING pathway. The speaker claims that the presence of an SV40 origin of replication, a mammalian origin, in a vaccine grown in E. coli is reckless because it allows the plasmid DNA to replicate episomally in the host. The speaker alleges evidence suggests Pfizer, unlike Moderna, may have included this origin of replication due to carelessness. The speaker highlights concerns about nucleic acid persistence, noting that RT-PCR methods used in studies like Krausson and Rolchen may have amplified both DNA and RNA. The speaker suggests that prior studies assumed detected nucleic acids were RNA, but that further investigation using primers specific to the plasmid backbone might reveal the presence of residual plasmid DNA. The Krausson paper found nucleic acids present for thirty days in heart tissues, and the Rolchen paper found them for sixty days.

Moderna holds a patent for using RNA in vaccines, acknowledging that RNA is superior to DNA due to concerns about DNA-related problems like insertional immunogenesis and genotoxicity. The FDA claims to be unaware of any issues, but Moderna's own patent raises the same concerns about DNA. It appears that DNA is present in the RNA preparation as a contaminant, as it is used in the process of making RNA. Recent findings by scientists revealed large numbers of DNA fragments in the RNA preparation, including sequences that are not normally allowed in human use, such as an antibiotic resistance gene and sequences from simian virus 40. These DNA fragments can potentially lead to DNA damage, birth defects, and cancer.

The Pfizer and Moderna vaccines contain fragments of DNA, which can integrate into the genomic DNA of cells and become a permanent part of the cell. This poses a potential risk of autoimmune attacks and future cancer. The DNA contamination occurred during the production process, where a plasmid vector was used to scale up the production of the RNA template. The regulatory threshold for DNA in vaccines is outdated and not suitable for this new type of vaccine. The speaker believes that DNA sequencing should be done on vaccinated individuals' stem cells to determine if this theoretical risk has occurred. Informed consent is necessary, and the lack of transparency regarding the DNA contamination is concerning.

The presence of DNA plasmids and undisclosed proteins in the Pfizer COVID-19 vaccine has raised concerns. The DNA plasmids, originating from E. Coli, were not properly removed during manufacturing, resulting in contamination. Additionally, two proteins from the simian virus 40 (SV40) were found in the vaccine, which is associated with certain cancers. SV40 was present in polio vaccines administered to millions of Americans in the past. Injecting these proteins and DNA into the body can potentially lead to mutations and increased risk of cancer. This discovery suggests a higher chance of mutation from the Pfizer COVID-19 vaccine.

Moderna holds a patent for using RNA in vaccines, acknowledging that RNA is better than DNA due to concerns about DNA-related problems like insertional immunogenesis and genotoxicity. The FDA claims to be unaware of these concerns, but Moderna's own patent highlights them. The presence of DNA in the vaccines is considered a contaminant, as it is used in the process of making RNA. Recent findings by scientists in the US and Canada revealed large amounts of DNA fragments in the RNA preparation, including sequences not allowed for human use, such as an antibiotic resistance gene and sequences from simian virus 40. These DNA fragments pose risks of DNA damage, including birth defects and cancer.

When COVID-19 vaccines were sequenced, commercial annotation software highlighted functional parts of the plasmids, including antibiotic resistance genes and SV40 components. The speaker claims that Pfizer had to manually remove these annotations before submitting the plasmid map to regulators. According to the speaker, regulators received the DNA sequence, but the sponsor is obligated to annotate every open reading frame and promoter, even if their function is unknown. The speaker alleges that Pfizer intentionally removed annotations, hiding them from the FDA, which the speaker believes is a violation of guidelines. The speaker suggests the reason for hiding SV40 components is due to SV40 virus contamination in polio vaccines and its debated link to cancer. The speaker asserts that while epidemiological data is confounded by vaccine shedding, laboratory studies show SV40 is a potent oncogenic virus. The speaker claims that the vaccines contain some of the more carcinogenic components of that virus, and that these sequences are functional and have consequences.

Mr. Kevin McKernan, a former leader at the Human Genome Project, discussed DNA contamination in Pfizer and Moderna vaccines. He highlighted the risks of insertional mutagenesis and integration into the genome, contradicting regulators' claims of little consequence. The DNA, found in lipid nanoparticles, can enter cells and potentially contribute to cancer. McKernan emphasized the inadequacy of current monitoring methods and called for a review of regulatory practices. The presence of DNA in these vaccines challenges existing safety standards and raises concerns about long-term effects.

Many labs, including Medicinal Genomics, found DNA contamination in Pfizer and Moderna mRNA vaccines. Regulators like the FDA and EMA admitted to this, but downplayed its significance. The SP 40 sequences omitted by Pfizer are crucial. DNA contamination can cause insertional mutagenesis, as stated in Moderna's patents. Regulatory agencies were deceived and failed to properly address the issue. This poses a serious risk that cannot be ignored.

The Pfizer vaccine may contain DNA in addition to mRNA, according to a scientist who sequenced the vaccine in their lab. The DNA is a vector used in the production of the mRNA. The scientist examined vials of the vaccine from a local vaccination program and found DNA in them. This DNA could potentially be linked to rare but serious side effects, such as death from cardiac arrest. It has the ability to integrate into the genomic DNA of cells, potentially causing long-term effects. The presence of this DNA raises concerns about the regulatory process that allowed it to be included in the vaccine. There is a theoretical risk of autoimmune reactions and future cancer development. Further investigation is needed to determine the extent of these risks.

I'm Philippe Boucalt, a cancer genomics researcher at the University of South Carolina. I've sequenced the DNA in the Pfizer vaccine and found that it contains fragments of DNA. This DNA could potentially cause rare but serious side effects, such as cardiac arrest and future cancer risks. The regulatory process that allowed this contamination is concerning. The DNA could integrate into long-lived somatic cells and potentially cause autoimmune attacks or disrupt tumor suppressors. To produce the vaccine, they cloned the PCR product into a plasmid vector, which led to the contamination. We can easily measure the amount of this substance in the vaccine and should conduct further studies to understand its implications. The FDA should require Pfizer to remove the DNA from the vaccine.

"Pfizer vaccine is contaminated with plasma DNA. It's not just mRNA." "This DNA is the DNA vector that was used as the template for the in vitro transcription reaction when they made the mRNA." "I sequenced it in my own lab." "The vials of Pfizer vaccine that were given out here in Colombia, one of my colleagues was in charge of that vaccination program in the College of Pharmacy." "And for reasons that I still don't understand, he kept every single vial." "So he had a whole freezer full of the empty vials." "And I checked these two batches, and I checked them by sequencing." "It's surprising that there's any DNA in there." "This DNA, in my view, it could be causing some of the rare but serious side effects like death from cardiac arrest."

We were surprised by the findings from a patient vaccinated four times with Pfizer. A year post-vaccination, tumors developed, and the patient died within a month. Biopsies revealed SV40 in the vaccine's origin of replication. Preliminary sequencing confirmed the presence of the Pfizer vaccine's DNA, including sequences from Spike protein. Unexpectedly, the DNA copy number in the tumors exceeded that of the human genome, indicating a significant insertional mutagenesis event. Instead of the anticipated lower mutation rates, our PCR results showed signals as strong as the original vaccine vial, suggesting the DNA is replicating within the patient. This indicates that the mammalian origin of replication in Pfizer's vaccine is active in human tumors.

A colon biopsy from a Pfizer-vaccinated individual who died a month after tumor emergence revealed SV40 in the origin of replication from the Pfizer vaccine. Preliminary sequencing suggests the presence of Pfizer's vaccine. The vaccine DNA copy number was greater than the human genome, indicating replication. PCR signals suggest the vaccine concentration is similar to or higher than the original vial, despite dilution in the body. This indicates the mammalian origin of replication in Pfizer's vaccine is active in human tumors. This finding may explain shedding, which is not supposed to occur because the COVID vaccine is not intended to alter DNA. However, evidence suggests that the vaccine may be altering DNA, making it a VGBT (genome altering) product. This could mean that spike proteins are continually created, and shedding is real.

Health Canada initially claimed the Pfizer vaccine did not contain SV40, but later acknowledged its presence, stating it has no functional role. Despite this, they sought clarification from Pfizer about the residual fragments. When asked for information, Health Canada provided redacted documents, raising questions about transparency. A technique called fluorometry, which labels DNA with fluorescent markers, revealed that some RNA vaccines, particularly Moderna's, could contain up to 10 trillion copies of DNA fragments per dose. This is concerning because just 3 to 10 copies can facilitate the incorporation of SV40 DNA into a cell's nuclear genome, potentially leading to mutations associated with cancer.

A peer-reviewed paper confirms Pfizer's mRNA vaccine is contaminated with DNA and SV40 enhancers, considered dangerous. Pfizer used bacterial plasma DNA during mass production, leading to contamination with spike protein genes, antibiotic resistance markers, and SV40 enhancers. Researchers found 4-5 times more DNA than the safe limit. The DNA fragments could integrate into the human genome due to the SV40 enhancer. Injected vaccine samples caused super strong kidney cells to produce spike protein, excreted via exosomes, potentially spreading body-wide. The vaccines were toxic to these cells, causing pathological changes. The DNA, tucked into lipid nanoparticles, could integrate into the human genome, turning the jab into accidental gene therapy. The SV40 enhancer drags DNA into the nucleus. The study suggests the inclusion of SV40 enhancers was deliberate, not accidental, and scrubbed from regulatory paperwork. Experts theorize this contamination could be linked to an explosion of turbo cancers. The study concludes mRNA shots should be suspended until safety is determined. Another study found vaccine spike protein expressed in cerebral arteries of stroke patients for up to 17 months, accompanied by an autoimmune response.

The Pfizer vaccine may contain DNA in addition to mRNA, according to a scientist who sequenced the vaccine in his lab. He obtained empty vials from a colleague and found DNA in them. This DNA could potentially cause serious side effects and integrate into the genomic DNA of cells, leading to long-term effects. The scientist is concerned about the regulatory process that allowed this to happen and warns of the risks of genome modification and autoimmune attacks. While the risk of cancer is believed to be rare, it is not zero. Further investigation is needed to determine the extent of these risks.

A peer-reviewed paper confirms Pfizer's mRNA vaccine is contaminated with dangerous DNA and SV40 enhancers. During production, bacterial plasmid DNA led to contamination with spike protein genes, antibiotic resistance markers, and SV40 enhancers. Researchers found four to five times more DNA than the safe limit; these DNA fragments could integrate into the human genome due to the SV40 enhancer. Experiments showed the vaccines were toxic to cells, causing pathological changes and spike protein production, potentially spreading body-wide via exosomes. The DNA contamination, tucked into lipid nanoparticles, could lead to unintended gene therapy. The SV40 enhancer, deliberately added but removed from regulatory paperwork, raises questions about Pfizer's intentions. Experts theorize this contamination could be linked to an increase in "turbo cancers," coinciding with Pfizer's acquisition of a cancer drugmaker. An epidemiologist noted the study found DNA from the manufacturing process way over regulatory limits, including the cancer-promoting SV40 promoter enhancer and spike-producing DNA. A separate study found vaccine spike protein expressed in cerebral arteries of stroke patients for up to seventeen months, potentially contributing to autoimmune responses. There are now 11 independent reports that have found, DNA contamination within these shots of up to 60000% above regulatory limits.

Alden and colleagues found that Pfizer's genetic code can be integrated into the human genome within an hour in a cancerous cell line. This suggests that Pfizer and Moderna's genetic material might become a permanent part of human DNA. There is no study confirming or denying this possibility. The concern is that if eggs or sperm incorporate this genetic code, it could be passed on to future generations. This lack of research is seen as reckless and worrisome.

There is significant DNA contamination found in vaccines, with evidence from multiple researchers in Germany, Japan, and the U.S. Regulatory bodies like the FDA and EMA acknowledge this contamination but downplay its significance, relying on Pfizer's assurances. The clinical trials used cleaner DNA, but the mass-produced vaccines did not undergo the same purification, leading to increased background DNA and endotoxin levels. Regulators received a plasmid map missing crucial annotations, suggesting manipulation. Claims about expired vials and PCR methods used to measure contamination have been challenged, with evidence showing that Moderna's vaccines are cleaner. Regulators are allowing different measurement standards for RNA and DNA, raising concerns about transparency and integrity in the regulatory process.

The Pfizer vaccine contains not only mRNA but also plasma DNA from the vector used in its production. I sequenced samples from two batches of the vaccine in Colombia and found this DNA, which raises concerns about potential health risks. This DNA could integrate into the genomic DNA of cells, leading to permanent changes. Such integration poses theoretical risks, including autoimmune responses and cancer, depending on where the DNA inserts itself in the genome. While these risks may be rare, they warrant investigation to understand their implications better.

The panel discusses replication (replicon) vaccines and their potential dangers, focusing on how they differ from conventional messenger RNA (mRNA) vaccines and what new risks might emerge as this technology develops. Key points and concerns raised - Replicon vaccines concept and fundamental differences - Replicon vaccines use replication-capable genetic material, so the embedded genetic information not only makes antigen proteins but also multiplies inside the cell. They are described as having both constitutive function (the ability to make proteins) and, crucially, the capacity to replicate, which distinguishes them from traditional, non-replicating mRNA vaccines. - It is explained that replication introduces additional mutation and recombination opportunities, because the RNA genome is copied more than once, and the process can produce variants that differ from the original design. - Central dogma exceptions and viral biology - The speakers explain that while the central dogma (DNA → RNA → protein) generally governs biology, some viruses violate this, with RNA viruses that replicate via RNA-dependent replication and even some reverse-transcribing retroviruses that convert RNA to DNA and integrate into genomes. This context is used to frame why replicon vaccines could behave unpredictably. - Potential risks of replication and spread - A core concern is that the replicon approach might allow the vaccine genome to spread beyond the initial target cells, potentially reaching other cells and tissues, or even spreading to other people via exosomes or other means. Exosomes can transport DNA, RNA, and proteins between cells; thus, the replicon genome could in theory be disseminated. - The possibility of homologous or heterologous recombination between replicon genomes and wild-type viruses could yield new variants. The panel emphasizes the difficulty of controlling such recombination in a living system. - Specific material and design considerations - The use of viral components like spike protein genes in replicon vaccines raises concerns about how these proteins might mutate or recombine during replication, potentially altering antigen presentation or safety. - A concern is raised about the lack of repair mechanisms in RNA replication (as opposed to DNA replication), which could make error rates higher and lead to unpredictable changes. - The panel notes that current replicon vaccine designs (including those using alphavirus backbones) inherently carry high mutation and recombination risk, and that the replicating systems may encounter unpredictable evolutionary dynamics inside the human body. - Safety signals and clinical anecdotes - The speakers cite cases of adverse events temporally associated with vaccines, including vascular inflammation and thrombosis, stroke-like events, and myocarditis, to illustrate that immune responses to vaccines can be complex and occasionally severe. They emphasize that such observations do not establish causality, but argue they warrant careful scrutiny. - There are references to cases of acute vascular and neural complications following repeated vaccination, and to broader immune dysregulation phenomena, including IGG4-related disease and immune dysregulation syndromes that can involve multiple organs. - One example concerns a patient who developed sudden limb problems after the third dose, requiring surgery; another describes myocardial involvement after multiple doses and subsequent inflammatory sequelae. - DNA contamination and analytical findings - Kevin McKernan’s analysis of certain Japanese CoronaVac vaccines is cited: both DNA contamination and the presence of SV40 promoter elements were detected in some vaccine lots, with DNA amounts exceeding some regulatory benchmarks in at least one case. The concern is that DNA contamination, or the presence of promoter sequences, could influence integration or expression in unintended ways. - It is noted that vaccines using lipid nanoparticles can potentially deliver nucleic acids into cells; in the presence of exons or promoter sequences, there could be unintended cellular uptake and expression. - Implications for public health and policy - The panel underscores the need for caution, thorough investigation, and long-term observation of any replication-based vaccine platform before broad deployment. There is a call to evaluate risks, monitor long-term outcomes, and consider the possibility that replication-competent constructs could drive unforeseen evolutionary dynamics within hosts or communities. - There is contention about how information is communicated to the public, with particular emphasis on avoiding misinformation while ensuring that scientific uncertainties are transparently discussed. - Broader scientific context and forward-looking stance - The speakers discuss how the field’s approach to gene-based vaccines is evolving rapidly, and they stress that the compatibility of replicon systems with human biology is not yet fully understood. - They frame their discussion as not merely about current vaccines but about the trajectory of vaccine platforms: if replication-based or self-dispersing systems prove too risky or unpredictable, the prudent path might be to favor conventional, non-replicating strategies until safety, efficacy, and containment of unintended spread are more firmly established. Closing and takeaways - The session closes with emphasis on careful evaluation of replicon vaccines, awareness that viral genetics can behave differently in humans than in theory, and a call for continued discussion, independent verification, and transparent communication as the technology develops. - Throughout, speakers acknowledge the complexity of immune responses to vaccines, the potential for unexpected adverse events, and the importance of safeguarding public health while advancing vaccine science.

Pfizer and Moderna vaccines use two processes. The first process involves using PCR to amplify and create DNA for clinical trials. Once approved, they use circular bacterial DNA plasmid to replicate billions of mRNA DNA sample copies. However, this resulted in contaminated vaccines with junk DNA. A study found DNA fragments in Pfizer and Moderna vaccines in Ontario, Canada. Researchers tested 27 mRNA vials from 12 different lots and discovered billions to 100 billions of DNA molecules per dose, exceeding FDA and WHO guidelines by 188 to 509 times. This is a significant amount, far beyond what is acceptable.

The Pfizer vaccine may contain DNA in addition to mRNA, according to a researcher who sequenced the vaccine in their lab. The DNA is a vector used in the production of the mRNA. The researcher expressed concern about the potential consequences of this, including rare but serious side effects like death from cardiac arrest. The DNA could integrate into the genomic DNA of cells and become a permanent part of them, posing a risk of genome modification and autoimmune attacks. There is also a theoretical risk of future cancer depending on where the foreign DNA lands in the genome. The researcher believes further investigation is needed to determine if these risks are occurring.

The Pfizer vaccine is contaminated with plasma DNA, not just mRNA. This DNA is the DNA vector used as the template for the in vitro transcription reaction. This was discovered by sequencing vials of Pfizer vaccine from Colombia. It's surprising that there's any DNA in there. The speaker is alarmed about the possible consequences of this, including rare but serious side effects like death from cardiac arrest. Mixing DNA with a lipid complex allows it to enter cells and become a permanent fixture. This is a real hazard for genome modification of long-lived somatic cells, like stem cells, and could cause a sustained autoimmune attack. There is also a very real theoretical risk of future cancer in some people. The risk is not zero and it may be high enough that we ought to figure out if this is happening or not.

The Pfizer vaccine contains DNA contamination in addition to mRNA. The DNA comes from the DNA vector used as a template for making the mRNA. Sequencing analysis of the vaccine revealed the presence of DNA, which could potentially cause serious side effects and integrate into the genomic DNA of cells. This poses risks such as autoimmune attacks and potential future cancer. The DNA contamination likely occurred during the production process. It is important to investigate if this DNA has integrated into the genomes of vaccinated individuals. The FDA should require Pfizer to remove the DNA from future versions of the vaccine. The regulatory limit for DNA in vaccines is outdated and not suitable for this type of vaccine. It is necessary to address this oversight and ensure the safety of the vaccine.