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Quan et al demonstrated that the introduction of DNA into a cell, even without integration, can trigger the oncogenic cGAS-STING pathway. The speaker claims that the presence of an SV40 origin of replication, a mammalian origin, in a vaccine grown in E. coli is reckless because it allows the plasmid DNA to replicate episomally in the host. The speaker alleges evidence suggests Pfizer, unlike Moderna, may have included this origin of replication due to carelessness. The speaker highlights concerns about nucleic acid persistence, noting that RT-PCR methods used in studies like Krausson and Rolchen may have amplified both DNA and RNA. The speaker suggests that prior studies assumed detected nucleic acids were RNA, but that further investigation using primers specific to the plasmid backbone might reveal the presence of residual plasmid DNA. The Krausson paper found nucleic acids present for thirty days in heart tissues, and the Rolchen paper found them for sixty days.

SV40 promoter sequences are still detectable 48 hours later, despite being wrapped in LNPs and using methods aimed at eliminating them. This raises urgent concerns about how long this DNA persists without DNA depletion protocols. The purification methods used to capture this DNA require thorough examination, as techniques like ethanol precipitation and mini DNA purification kits may not effectively eliminate small DNA fragments or could further degrade them.

Moderna holds a patent for using RNA in vaccines, acknowledging that RNA is superior to DNA due to concerns about DNA-related problems like insertional immunogenesis and genotoxicity. The FDA claims to be unaware of any issues, but Moderna's own patent raises the same concerns about DNA. It appears that DNA is present in the RNA preparation as a contaminant, as it is used in the process of making RNA. Recent findings by scientists revealed large numbers of DNA fragments in the RNA preparation, including sequences that are not normally allowed in human use, such as an antibiotic resistance gene and sequences from simian virus 40. These DNA fragments can potentially lead to DNA damage, birth defects, and cancer.

The Pfizer and Moderna vaccines contain fragments of DNA, which can integrate into the genomic DNA of cells and become a permanent part of the cell. This poses a potential risk of autoimmune attacks and future cancer. The DNA contamination occurred during the production process, where a plasmid vector was used to scale up the production of the RNA template. The regulatory threshold for DNA in vaccines is outdated and not suitable for this new type of vaccine. The speaker believes that DNA sequencing should be done on vaccinated individuals' stem cells to determine if this theoretical risk has occurred. Informed consent is necessary, and the lack of transparency regarding the DNA contamination is concerning.

When COVID-19 vaccines were sequenced, commercial annotation software highlighted functional parts of the plasmids, including antibiotic resistance genes and SV40 components. The speaker claims that Pfizer had to manually remove these annotations before submitting the plasmid map to regulators. According to the speaker, regulators received the DNA sequence, but the sponsor is obligated to annotate every open reading frame and promoter, even if their function is unknown. The speaker alleges that Pfizer intentionally removed annotations, hiding them from the FDA, which the speaker believes is a violation of guidelines. The speaker suggests the reason for hiding SV40 components is due to SV40 virus contamination in polio vaccines and its debated link to cancer. The speaker asserts that while epidemiological data is confounded by vaccine shedding, laboratory studies show SV40 is a potent oncogenic virus. The speaker claims that the vaccines contain some of the more carcinogenic components of that virus, and that these sequences are functional and have consequences.

Many labs, including Medicinal Genomics, found DNA contamination in Pfizer and Moderna mRNA vaccines. Regulators like the FDA and EMA admitted to this, but downplayed its significance. The SP 40 sequences omitted by Pfizer are crucial. DNA contamination can cause insertional mutagenesis, as stated in Moderna's patents. Regulatory agencies were deceived and failed to properly address the issue. This poses a serious risk that cannot be ignored.

Chakrabarty reviewed the Ryan et al and Odek studies, mapping reads to plasmids and finding significant spike sequence from RNA, and less from plasmid DNA, which is expected. RNA sequencing protocols suppress DNA, yet DNA is still present. The Odek study shows the entire vector backbone covered with sequencing reads, indicating heavy contamination and the presence of SV40 promoters in patients. This is evidenced across multiple studies. The Novel study had a lighter density of reads, but some plasmid DNA was detectable. The Lee et al study also showed some SV40 reads. These are more apparent in samples taken closer to vaccination, despite DNA suppression methods. A mice study on vaccine redentilation showed poly A tails regenerate, potentially lengthening RNA lifespan, but DNA contamination was also present.

- The mRNA on plasmids was produced, and after processing, much DNA from plasmids remained; Kevin MacKinnon found that vials were full of plasmid DNA, the whole plasmid and parts of it, and this was published. Authorities claimed it doesn’t matter and that vaccines have saved millions of lives, so why not have some DNA in them. - The DNA in the vaccine vials was packaged in lipid nanoparticles and was shown by colleagues last year (the INMODIA publication) to enter human cells in culture and remain stable in cells for days, as did the mRNA. Despite this, the message given was to poof, never mind, don’t worry, be happy. - A radical change occurred due to a discovery by Kevin MacKinnon three weeks ago: during transcription on the chromosome, byproducts are generated; some mRNA strands do not detach from the DNA where they’re formed, creating hybrids of DNA and RNA that come off together. These hybrids are dangerous. - In cells, an enzyme called RNase H takes care of these sparks and extinguishes them immediately; otherwise they can cause damage to the chromosome, potentially lighting “fires” on the chromosomes. If not extinguished, the fires can cause diverse damage depending on where they occur, potentially leading to illnesses described in medical textbooks, including tumors (neoplastic disease), autoimmune disease, developmental impairment, birth defects, or death. - The speaker asserts these hybrids and their mishandling could lead to a broad range of illnesses, and emphasizes that this situation is not limited to the COVID vaccine but applies to all Moderna RNA vaccines, including new Moderna RNA vaccines entering the market, such as a flu vaccine, and mentions veterinary RNA vaccines as well. - The claim is made that these vaccines will be heavily contaminated with deadly dangerous hybrids, and it is the duty of authorities and controlling authorities to stop proceeding and not turn away; otherwise they will face court for not fulfilling their duties. The speaker has been giving interviews and asserts this narrative is spreading worldwide, framing it as akin to attempted murder and urging physicians to refuse vaccination.

The amount of DNA allowed in vaccines was loosened a thousandfold after liability waivers were granted. These limits assumed naked DNA injection, which degrades quickly. However, mRNA vaccines use lipid nanoparticles (LNPs) that also coat contaminating DNA, invalidating the old limits. Studies show DNA levels in the shots are 10 to 100 times higher than the already obsolete limits. The LNPs deliver this DNA directly into cells, changing its persistence and biological impact. Pfizer used a different, more purified product in its trials than what was injected into billions of people. The initial process included a PCR amplification step to reduce DNA background, but this was dropped for mass production due to cost. This resulted in higher levels of background plasmid DNA and potentially E. coli components like endotoxin in the shots, possibly causing anaphylactic reactions. The presence of plasmids has been confirmed, and this process change, documented in the BMJ, is a major violation of manufacturing standards. The EMA requested a new trial after the process change, but the data was never delivered, rendering the original trial data irrelevant.

There is no question that the inclusion of DNA was not an accident, and the 1/3 DNA, 2/3 RNA ratio was intentional. The long-term effects of both DNA and RNA are influencing the ability to fight cancer. The key question is what effect the DNA has on the cell nucleus, and how to differentiate its damage from that of mRNA. mRNA is connected to ACE 2 receptors and causes damage throughout the reproductive and cardio systems. mRNA creates a spike protein flag, which the immune system attacks, leading to organ damage. The presence of DNA makes the process last longer. While mRNA would create spike for 8-12 months, DNA can influence the nucleus for years, producing mRNA near the nucleus. This extends the process from months to 8-10 years due to the decision to include DNA.

Key points: "the vials that were in fact approved are not the vials that were given to the public." The clinical trial used "process one that used PCR to make the DNA that was going to then turn into the RNA to make the spike protein." After the trial, "they switched" to a production process that "manufactured this DNA in E. Coli," introducing endotoxin risk. "There are these plasmids that have additional DNA that were not present in the actual clinical trial." Sequencing found mixtures, including "expired" and samples that had "been tapped into." Regarding Pfizer, "the Pfizer vaccines actually had a component that was not disclosed to the regulators." "The plasmid map on the right is what was disclosed to the EMA" with "no mention of the SV40 components" now "inside this DNA sequence." "The plasma on the left is what we actually found," with components not disclosed to regulators nor to patients. Monovalent Pfizer; prior ones were the bivalent vaccines from Moderna and Pfizer.

Pfizer's use of the RiboGreen technique to measure DNA in their vaccines has raised concerns about deceptive practices. The presence of billions of DNA fragments in each dose, some of which are small and more likely to integrate into the genome, is worrying. Preliminary data suggests a correlation between adverse events and contaminated Pfizer vaccines, but more research is needed. The DNA in the vaccines is different from previous contamination and carries a higher risk of integration. The FDA acknowledges the integration risk and the need for lower limits on DNA when copy numbers are high. The DNA is encapsulated in lipid nanoparticles, making it prothrombotic and potentially oncogenic. The presence of endotoxin and the spike protein in the vaccines further complicates the situation. The vaccines have been found in various tissues and can lead to prolonged expression of the spike protein. Insertional mutagenesis and cancer risk are concerns, especially for individuals with weakened immune systems. Regulatory bodies have confirmed the presence of the SV40 sequence in the vaccines, but the clinical implications are still unclear.

The initial regulatory response claimed the DNA fragment was too small to matter, but this was based on an assumption without measurement. The quantity of DNA is now shown to be over the limit, especially considering lipid nanoparticles, even if the limit were justifiable. Claims that the DNA is non-functional are also incorrect. The DNA sequence includes the SV40 promoter and enhancer region, as well as an SV40 origin of replication.

"Pfizer vaccine is contaminated with plasma DNA. It's not just mRNA." "This DNA is the DNA vector that was used as the template for the in vitro transcription reaction when they made the mRNA." "I sequenced it in my own lab." "The vials of Pfizer vaccine that were given out here in Colombia, one of my colleagues was in charge of that vaccination program in the College of Pharmacy." "And for reasons that I still don't understand, he kept every single vial." "So he had a whole freezer full of the empty vials." "And I checked these two batches, and I checked them by sequencing." "It's surprising that there's any DNA in there." "This DNA, in my view, it could be causing some of the rare but serious side effects like death from cardiac arrest."

Pfizer and Moderna used two processes to create their vaccines. Initially, they used PCR to amplify and create the DNA for clinical trials. However, when they received approval, they needed to produce billions of copies, so they used circular bacterial DNA plasmids. Unfortunately, this led to contamination with junk DNA. Researchers in Ontario, Canada tested 27 mRNA vials from 12 different lots and found billions to hundreds of billions of DNA molecules per dose, exceeding FDA and WHO guidelines by 188 to 509 times. This is a significant amount, far beyond what is considered acceptable.

There is significant DNA contamination found in vaccines, with evidence from multiple researchers in Germany, Japan, and the U.S. Regulatory bodies like the FDA and EMA acknowledge this contamination but downplay its significance, relying on Pfizer's assurances. The clinical trials used cleaner DNA, but the mass-produced vaccines did not undergo the same purification, leading to increased background DNA and endotoxin levels. Regulators received a plasmid map missing crucial annotations, suggesting manipulation. Claims about expired vials and PCR methods used to measure contamination have been challenged, with evidence showing that Moderna's vaccines are cleaner. Regulators are allowing different measurement standards for RNA and DNA, raising concerns about transparency and integrity in the regulatory process.

The Pfizer vaccine contains not only mRNA but also plasma DNA from the vector used in its production. I sequenced samples from two batches of the vaccine in Colombia and found this DNA, which raises concerns about potential health risks. This DNA could integrate into the genomic DNA of cells, leading to permanent changes. Such integration poses theoretical risks, including autoimmune responses and cancer, depending on where the DNA inserts itself in the genome. While these risks may be rare, they warrant investigation to understand their implications better.

The panel discusses replication (replicon) vaccines and their potential dangers, focusing on how they differ from conventional messenger RNA (mRNA) vaccines and what new risks might emerge as this technology develops. Key points and concerns raised - Replicon vaccines concept and fundamental differences - Replicon vaccines use replication-capable genetic material, so the embedded genetic information not only makes antigen proteins but also multiplies inside the cell. They are described as having both constitutive function (the ability to make proteins) and, crucially, the capacity to replicate, which distinguishes them from traditional, non-replicating mRNA vaccines. - It is explained that replication introduces additional mutation and recombination opportunities, because the RNA genome is copied more than once, and the process can produce variants that differ from the original design. - Central dogma exceptions and viral biology - The speakers explain that while the central dogma (DNA → RNA → protein) generally governs biology, some viruses violate this, with RNA viruses that replicate via RNA-dependent replication and even some reverse-transcribing retroviruses that convert RNA to DNA and integrate into genomes. This context is used to frame why replicon vaccines could behave unpredictably. - Potential risks of replication and spread - A core concern is that the replicon approach might allow the vaccine genome to spread beyond the initial target cells, potentially reaching other cells and tissues, or even spreading to other people via exosomes or other means. Exosomes can transport DNA, RNA, and proteins between cells; thus, the replicon genome could in theory be disseminated. - The possibility of homologous or heterologous recombination between replicon genomes and wild-type viruses could yield new variants. The panel emphasizes the difficulty of controlling such recombination in a living system. - Specific material and design considerations - The use of viral components like spike protein genes in replicon vaccines raises concerns about how these proteins might mutate or recombine during replication, potentially altering antigen presentation or safety. - A concern is raised about the lack of repair mechanisms in RNA replication (as opposed to DNA replication), which could make error rates higher and lead to unpredictable changes. - The panel notes that current replicon vaccine designs (including those using alphavirus backbones) inherently carry high mutation and recombination risk, and that the replicating systems may encounter unpredictable evolutionary dynamics inside the human body. - Safety signals and clinical anecdotes - The speakers cite cases of adverse events temporally associated with vaccines, including vascular inflammation and thrombosis, stroke-like events, and myocarditis, to illustrate that immune responses to vaccines can be complex and occasionally severe. They emphasize that such observations do not establish causality, but argue they warrant careful scrutiny. - There are references to cases of acute vascular and neural complications following repeated vaccination, and to broader immune dysregulation phenomena, including IGG4-related disease and immune dysregulation syndromes that can involve multiple organs. - One example concerns a patient who developed sudden limb problems after the third dose, requiring surgery; another describes myocardial involvement after multiple doses and subsequent inflammatory sequelae. - DNA contamination and analytical findings - Kevin McKernan’s analysis of certain Japanese CoronaVac vaccines is cited: both DNA contamination and the presence of SV40 promoter elements were detected in some vaccine lots, with DNA amounts exceeding some regulatory benchmarks in at least one case. The concern is that DNA contamination, or the presence of promoter sequences, could influence integration or expression in unintended ways. - It is noted that vaccines using lipid nanoparticles can potentially deliver nucleic acids into cells; in the presence of exons or promoter sequences, there could be unintended cellular uptake and expression. - Implications for public health and policy - The panel underscores the need for caution, thorough investigation, and long-term observation of any replication-based vaccine platform before broad deployment. There is a call to evaluate risks, monitor long-term outcomes, and consider the possibility that replication-competent constructs could drive unforeseen evolutionary dynamics within hosts or communities. - There is contention about how information is communicated to the public, with particular emphasis on avoiding misinformation while ensuring that scientific uncertainties are transparently discussed. - Broader scientific context and forward-looking stance - The speakers discuss how the field’s approach to gene-based vaccines is evolving rapidly, and they stress that the compatibility of replicon systems with human biology is not yet fully understood. - They frame their discussion as not merely about current vaccines but about the trajectory of vaccine platforms: if replication-based or self-dispersing systems prove too risky or unpredictable, the prudent path might be to favor conventional, non-replicating strategies until safety, efficacy, and containment of unintended spread are more firmly established. Closing and takeaways - The session closes with emphasis on careful evaluation of replicon vaccines, awareness that viral genetics can behave differently in humans than in theory, and a call for continued discussion, independent verification, and transparent communication as the technology develops. - Throughout, speakers acknowledge the complexity of immune responses to vaccines, the potential for unexpected adverse events, and the importance of safeguarding public health while advancing vaccine science.

Pfizer and Moderna vaccines use two processes. The first process involves using PCR to amplify and create DNA for clinical trials. Once approved, they use circular bacterial DNA plasmid to replicate billions of mRNA DNA sample copies. However, this resulted in contaminated vaccines with junk DNA. A study found DNA fragments in Pfizer and Moderna vaccines in Ontario, Canada. Researchers tested 27 mRNA vials from 12 different lots and discovered billions to 100 billions of DNA molecules per dose, exceeding FDA and WHO guidelines by 188 to 509 times. This is a significant amount, far beyond what is acceptable.

Kevin discovered that the vials used for vaccines are contaminated with bacterial DNA. This is concerning because the modified RNA used in these vaccines creates unusual genetic structures that don't occur naturally. Normally, DNA is in a double helix form, but with modified RNA, there are three strands attached to the DNA. The enzyme used to remove DNA, called DNase, cannot digest these triple-stranded genetic constructs, resulting in DNA contamination in the shots. Pfizer and Moderna should have addressed this issue during the manufacturing process by using different enzymes. This shows that assumptions cannot be made when working with new, unnatural products. The DNA used to manufacture the modified RNA was not properly removed, leading to multiple scary aspects of contamination.

The Pfizer vaccine is contaminated with plasma DNA, not just mRNA. This DNA is the DNA vector used as the template for the in vitro transcription reaction. This was discovered by sequencing vials of Pfizer vaccine from Colombia. It's surprising that there's any DNA in there. The speaker is alarmed about the possible consequences of this, including rare but serious side effects like death from cardiac arrest. Mixing DNA with a lipid complex allows it to enter cells and become a permanent fixture. This is a real hazard for genome modification of long-lived somatic cells, like stem cells, and could cause a sustained autoimmune attack. There is also a very real theoretical risk of future cancer in some people. The risk is not zero and it may be high enough that we ought to figure out if this is happening or not.

The Pfizer vaccine contains DNA contamination in addition to mRNA. The DNA comes from the DNA vector used as a template for making the mRNA. Sequencing analysis of the vaccine revealed the presence of DNA, which could potentially cause serious side effects and integrate into the genomic DNA of cells. This poses risks such as autoimmune attacks and potential future cancer. The DNA contamination likely occurred during the production process. It is important to investigate if this DNA has integrated into the genomes of vaccinated individuals. The FDA should require Pfizer to remove the DNA from future versions of the vaccine. The regulatory limit for DNA in vaccines is outdated and not suitable for this type of vaccine. It is necessary to address this oversight and ensure the safety of the vaccine.

The speaker discusses claims about modified RNA (MOD RNA) vaccines and DNA contamination in plasmids. They state that after creating MOD RNA on plasmids, the plasmid DNA remained and much of it could not be destroyed. They reference Kevin MacKurn’s discovery three years ago that vials were full of plasmid DNA, the whole plasmid and parts of it, and note that authorities allegedly minimized the issue, arguing that it doesn’t matter and that vaccines have saved millions of lives. The speaker asserts that the DNA in the vaccine vials was packaged in lipid nanoparticles and that this DNA would enter human cells. They reference colleagues’ publication last year (the INMODEO publication) showing that the DNA in the vaccine vials entered cells in culture and remained stable in cells for days, just as the MOD RNA did. Despite this, they say authorities dismissed the concerns with reassurance that nothing would happen. A pivotal point is attributed to a recent discovery by Kevin MacKinnon, claimed to be three weeks old, about what happens during transcription in the chromosome. The speaker explains that during production, byproducts occur and some mRNA strands do not detach from the DNA where they are formed, resulting in hybrids of DNA and RNA that come off together. The hybrids are described as dangerous, akin to “sparks of a sparkler,” and the speaker emphasizes that RNase H is an enzyme in the cell that takes care of these sparks and extinguishes them immediately. The speaker states that normal physicians don’t know about this, and they had to read up on RNase H after Jessica urged them to. The claimed consequence of failing to extinguish these hybrids is damage to the chromosome, with the metaphor that fires could light up and damage where they occur. The speaker asserts this could lead to “any illness that you see in the textbooks of medicine,” including tumors, neoplastic disease, autoimmune disease, developmental impairment, and death. They warn that the book of life—the genes and chromosomes—could be set on fire if these hybrids are not neutralized. The speaker says they have given interviews weekly, including one with Gary Null, and allege that this information is spreading worldwide. They claim that this situation is akin to attempted murder and exhort physicians globally not to participate, promising that those who do will be charged. They claim this issue is not limited to COVID vaccines but applies to all MOD RNA vaccines, including a flu MOD RNA vaccine now in use, and possibly veterinary vaccines, which they claim will be heavily contaminated with deadly dangerous hybrids. They urge authorities and controlling bodies to act, warning that they will face court if they fail to address the issue.

RNA sequencing of the Moderna vaccine's three prime ends suggests a possible mechanism for RNA persistence: in vivo re-adenylation. The data indicates plasmid DNA contamination despite efforts to reduce it. The data also reveals contamination from other mRNA vaccines in Moderna's pipeline. The speaker suggests that with widespread DNA sequencing capabilities, tolerating incorrect RNA sizes in vaccines is irresponsible. Sequencing before approval would have allowed for a better understanding of low RNA scores before global administration.

Kevin discovered that the vials used for vaccines are contaminated with bacterial DNA. This is concerning because the modified RNA used in these vaccines creates unusual genetic structures that don't occur naturally. Normally, DNA is in a double helix form, but with modified RNA, it forms triple strands that can't be easily removed with DNase. This contamination is a result of corners being cut during the manufacturing process. Enzymes that could have eliminated the DNA were not used. This shows that assumptions cannot be made when working with new, unnatural products. The DNA contamination comes from using it to manufacture the modified RNAs.