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We received four Pfizer vaccines, and the one we sequenced is different from any previously analyzed. This may be an earlier version that Pfizer modified later. The vaccine contains a common plasmid used in coronavirus research, which raises questions about its origin. While Pfizer is the likely source, it's possible it leaked from a lab. We found similarities and differences in the components of the vaccines. The contamination hypothesis is unlikely since the plasmids have never been handled in our labs, and formalin fixation prevents spike protein production. Pfizer could have multiple undisclosed plasmids, which would be concerning, especially since we found one in a colon cancer sample that produces spike protein at high levels, indicating it could be replication competent and potentially transmissible. Transmissible cancer is a significant issue.

Quan et al demonstrated that the introduction of DNA into a cell, even without integration, can trigger the oncogenic cGAS-STING pathway. The speaker claims that the presence of an SV40 origin of replication, a mammalian origin, in a vaccine grown in E. coli is reckless because it allows the plasmid DNA to replicate episomally in the host. The speaker alleges evidence suggests Pfizer, unlike Moderna, may have included this origin of replication due to carelessness. The speaker highlights concerns about nucleic acid persistence, noting that RT-PCR methods used in studies like Krausson and Rolchen may have amplified both DNA and RNA. The speaker suggests that prior studies assumed detected nucleic acids were RNA, but that further investigation using primers specific to the plasmid backbone might reveal the presence of residual plasmid DNA. The Krausson paper found nucleic acids present for thirty days in heart tissues, and the Rolchen paper found them for sixty days.

The SB40 sequence was not declared to regulators and poses a potential cancer risk because any DNA that promotes cell growth can lead to unregulated cell growth, which is cancer. Concerns have been raised about the link between the vaccine and cancer. It is crucial to study and sequence cancers that have developed after vaccination to determine if there is a connection. This remains an important unknown that needs urgent investigation.

The Pfizer and Moderna vaccines contain fragments of DNA, which can integrate into the genomic DNA of cells and become a permanent part of the cell. This poses a potential risk of autoimmune attacks and future cancer. The DNA contamination occurred during the production process, where a plasmid vector was used to scale up the production of the RNA template. The regulatory threshold for DNA in vaccines is outdated and not suitable for this new type of vaccine. The speaker believes that DNA sequencing should be done on vaccinated individuals' stem cells to determine if this theoretical risk has occurred. Informed consent is necessary, and the lack of transparency regarding the DNA contamination is concerning.

The presence of DNA plasmids and undisclosed proteins in the Pfizer COVID-19 vaccine has raised concerns. The DNA plasmids, originating from E. Coli, were not properly removed during manufacturing, resulting in contamination. Additionally, two proteins from the simian virus 40 (SV40) were found in the vaccine, which is associated with certain cancers. SV40 was present in polio vaccines administered to millions of Americans in the past. Injecting these proteins and DNA into the body can potentially lead to mutations and increased risk of cancer. This discovery suggests a higher chance of mutation from the Pfizer COVID-19 vaccine.

An article in Spectator Australia reported that DNA plasmids are contaminated with SV40 promoter sequences, which are oncogenic and used in cancer development in mice. The speaker questions why this is in an RNA vaccine, as the presence of DNA means it can integrate. Despite claims that the spike protein stays at the injection site, it has been identified everywhere, including in metastases from a colorectal cancer case where spike messenger RNA from the vaccine insertion was confirmed. The speaker was refused autopsies, even with consent from relatives, including a case of explosive melanoma after a booster shot. A report in The Lancet about deaths after vaccines was pulled, allegedly due to improper peer review. The speaker claims a media channel warned them that the government had made it a crime to criticize the vaccine program on the media.

Chakrabarty reviewed the Ryan et al and Odek studies, mapping reads to plasmids and finding significant spike sequence from RNA, and less from plasmid DNA, which is expected. RNA sequencing protocols suppress DNA, yet DNA is still present. The Odek study shows the entire vector backbone covered with sequencing reads, indicating heavy contamination and the presence of SV40 promoters in patients. This is evidenced across multiple studies. The Novel study had a lighter density of reads, but some plasmid DNA was detectable. The Lee et al study also showed some SV40 reads. These are more apparent in samples taken closer to vaccination, despite DNA suppression methods. A mice study on vaccine redentilation showed poly A tails regenerate, potentially lengthening RNA lifespan, but DNA contamination was also present.

The Pfizer vaccine may contain DNA in addition to mRNA, according to a scientist who sequenced the vaccine in their lab. The DNA is a vector used in the production of the mRNA. The scientist examined vials of the vaccine from a local vaccination program and found DNA in them. This DNA could potentially be linked to rare but serious side effects, such as death from cardiac arrest. It has the ability to integrate into the genomic DNA of cells, potentially causing long-term effects. The presence of this DNA raises concerns about the regulatory process that allowed it to be included in the vaccine. There is a theoretical risk of autoimmune reactions and future cancer development. Further investigation is needed to determine the extent of these risks.

I'm Philippe Boucalt, a cancer genomics researcher at the University of South Carolina. I've sequenced the DNA in the Pfizer vaccine and found that it contains fragments of DNA. This DNA could potentially cause rare but serious side effects, such as cardiac arrest and future cancer risks. The regulatory process that allowed this contamination is concerning. The DNA could integrate into long-lived somatic cells and potentially cause autoimmune attacks or disrupt tumor suppressors. To produce the vaccine, they cloned the PCR product into a plasmid vector, which led to the contamination. We can easily measure the amount of this substance in the vaccine and should conduct further studies to understand its implications. The FDA should require Pfizer to remove the DNA from the vaccine.

Key points: "the vials that were in fact approved are not the vials that were given to the public." The clinical trial used "process one that used PCR to make the DNA that was going to then turn into the RNA to make the spike protein." After the trial, "they switched" to a production process that "manufactured this DNA in E. Coli," introducing endotoxin risk. "There are these plasmids that have additional DNA that were not present in the actual clinical trial." Sequencing found mixtures, including "expired" and samples that had "been tapped into." Regarding Pfizer, "the Pfizer vaccines actually had a component that was not disclosed to the regulators." "The plasmid map on the right is what was disclosed to the EMA" with "no mention of the SV40 components" now "inside this DNA sequence." "The plasma on the left is what we actually found," with components not disclosed to regulators nor to patients. Monovalent Pfizer; prior ones were the bivalent vaccines from Moderna and Pfizer.

The speaker discusses the finding of plasmids in Pfizer vaccines, referencing a widely read paper that initiated inquiry into the consequences of this residual contaminant. David Speaker replicated this work in Canada, finding it in every one of over 30 vials. Philip Buchholz replicated this in South Carolina, and Dr. Sid Lee replicated the work using different primers and Sanger sequencing. Bridget Koning has replicated this in Germany, and several federal agencies have admitted the presence of DNA, though disagreeing on clinical implications. Ulrich Kammerer's lab replicated the work, transfecting plasmids from the vaccines into cell lines, where they persisted for several cycles of cell division. High schoolers interning at the FDA White Oak facility also measured it and found it to be significantly over the limit. Numerous studies have replicated these findings, with most finding levels over the limit. One individual with ties to Moderna claims it is not over the limit. Kaiser et al. claimed to have found it slightly over the limit but deemed it inconsequential; however, their DNA isolation method has been refuted by Kommer and Konig.

Pfizer's use of the RiboGreen technique to measure DNA in their vaccines has raised concerns about deceptive practices. The presence of billions of DNA fragments in each dose, some of which are small and more likely to integrate into the genome, is worrying. Preliminary data suggests a correlation between adverse events and contaminated Pfizer vaccines, but more research is needed. The DNA in the vaccines is different from previous contamination and carries a higher risk of integration. The FDA acknowledges the integration risk and the need for lower limits on DNA when copy numbers are high. The DNA is encapsulated in lipid nanoparticles, making it prothrombotic and potentially oncogenic. The presence of endotoxin and the spike protein in the vaccines further complicates the situation. The vaccines have been found in various tissues and can lead to prolonged expression of the spike protein. Insertional mutagenesis and cancer risk are concerns, especially for individuals with weakened immune systems. Regulatory bodies have confirmed the presence of the SV40 sequence in the vaccines, but the clinical implications are still unclear.

"Pfizer vaccine is contaminated with plasma DNA. It's not just mRNA." "This DNA is the DNA vector that was used as the template for the in vitro transcription reaction when they made the mRNA." "I sequenced it in my own lab." "The vials of Pfizer vaccine that were given out here in Colombia, one of my colleagues was in charge of that vaccination program in the College of Pharmacy." "And for reasons that I still don't understand, he kept every single vial." "So he had a whole freezer full of the empty vials." "And I checked these two batches, and I checked them by sequencing." "It's surprising that there's any DNA in there." "This DNA, in my view, it could be causing some of the rare but serious side effects like death from cardiac arrest."

We were surprised by the findings from a patient vaccinated four times with Pfizer. A year post-vaccination, tumors developed, and the patient died within a month. Biopsies revealed SV40 in the vaccine's origin of replication. Preliminary sequencing confirmed the presence of the Pfizer vaccine's DNA, including sequences from Spike protein. Unexpectedly, the DNA copy number in the tumors exceeded that of the human genome, indicating a significant insertional mutagenesis event. Instead of the anticipated lower mutation rates, our PCR results showed signals as strong as the original vaccine vial, suggesting the DNA is replicating within the patient. This indicates that the mammalian origin of replication in Pfizer's vaccine is active in human tumors.

A colon biopsy from a Pfizer-vaccinated individual who died a month after tumor emergence revealed SV40 in the origin of replication from the Pfizer vaccine. Preliminary sequencing suggests the presence of Pfizer's vaccine. The vaccine DNA copy number was greater than the human genome, indicating replication. PCR signals suggest the vaccine concentration is similar to or higher than the original vial, despite dilution in the body. This indicates the mammalian origin of replication in Pfizer's vaccine is active in human tumors. This finding may explain shedding, which is not supposed to occur because the COVID vaccine is not intended to alter DNA. However, evidence suggests that the vaccine may be altering DNA, making it a VGBT (genome altering) product. This could mean that spike proteins are continually created, and shedding is real.

Recent findings suggest that DNA contamination in COVID jabs may act as a self-replicating infectious agent. Initially believed to be RNA, evidence shows billions of DNA copies coding for the spike protein in the vials. If this DNA integrates into bacteria or human cells, it could replicate and spread, potentially causing mutations linked to cancer. A case study revealed a patient, who had received four jabs, developed cancer with high levels of plasmid DNA in their tumor. This raises concerns about gut health and the inflammatory nature of the spike protein. It’s crucial to be proactive about detoxifying the gut and maintaining health. Resources and protocols for addressing these issues are available on my website.

Human cells immersed in vaccines immediately took up the vaccines, DNA, and bacterial chromosomes, and started producing spike protein in large quantities. The spike protein is stable, shed into the blood, and measurable. Plasmid and spike production continues for many days. Uptake of a foreign chromosome equates to genetic modification, and an instant moratorium on vaccines is demanded until manufacturers and regulatory authorities can guarantee they will not genetically modify people. Modified RNAs and plasmids don't need to integrate into human chromosomes to genetically modify cells; functioning within the cell for extended periods is sufficient. Chromosomal integration of fragments into the "book of life" can smear pages, preventing them from being read, which could explain the worldwide explosion of tumors. Human cells transfected were sent to Kevin McKernan, who found chromosomal integration of bacterial DNA in the human cells' chromosomes. This information can be used to demand action, including from governments.

The Pfizer vaccine may contain DNA in addition to mRNA, according to a scientist who sequenced the vaccine in his lab. He obtained empty vials from a colleague and found DNA in them. This DNA could potentially cause serious side effects and integrate into the genomic DNA of cells, leading to long-term effects. The scientist is concerned about the regulatory process that allowed this to happen and warns of the risks of genome modification and autoimmune attacks. While the risk of cancer is believed to be rare, it is not zero. Further investigation is needed to determine the extent of these risks.

Pfizer and Moderna used two processes to create their vaccines. Initially, they used PCR to amplify and create the DNA for clinical trials. However, when they received approval, they needed to produce billions of copies, so they used circular bacterial DNA plasmids. Unfortunately, this led to contamination with junk DNA. Researchers in Ontario, Canada tested 27 mRNA vials from 12 different lots and found billions to hundreds of billions of DNA molecules per dose, exceeding FDA and WHO guidelines by 188 to 509 times. This is a significant amount, far beyond what is considered acceptable.

The Pfizer vaccine contains not only mRNA but also plasma DNA from the vector used in its production. I sequenced samples from two batches of the vaccine in Colombia and found this DNA, which raises concerns about potential health risks. This DNA could integrate into the genomic DNA of cells, leading to permanent changes. Such integration poses theoretical risks, including autoimmune responses and cancer, depending on where the DNA inserts itself in the genome. While these risks may be rare, they warrant investigation to understand their implications better.

Spike protein and multi-organ inflammation were detected in vaccinees, associated with severe illness. The discovery of contaminating plasmid DNA in the vaccines provides a possible explanation. Kevin McKernan found that the crucial step of removing plasmid DNA had not been properly done, and his work has been replicated. Cell uptake of plasmids alters DNA instructions, equating to genome alteration. Foreign DNA can become encased with chromosomes in newly formed nuclei. Continued production of non-self protein provokes long-term inflammation and organ damage, including heart damage and neurological/psychiatric issues. Vaccine-induced heart disease is increasingly common. Autoimmune diseases can develop in different organs simultaneously. Vaccines accumulate in reproductive organs, potentially impairing fertility. Placental damage may enable genes to enter fetal circulation. Stem cells in umbilical cord blood are reduced and impaired following vaccination. Breastfeeding may result in direct passage of vaccines into the baby's blood. Packaged DNA fragments found as vaccine contaminants can insert into chromosomal DNA, potentially leading to cancer, developmental defects, and inheritable mutations. Contamination of RNA vaccines with plasmid DNA must be expected.

The panel discusses replication (replicon) vaccines and their potential dangers, focusing on how they differ from conventional messenger RNA (mRNA) vaccines and what new risks might emerge as this technology develops. Key points and concerns raised - Replicon vaccines concept and fundamental differences - Replicon vaccines use replication-capable genetic material, so the embedded genetic information not only makes antigen proteins but also multiplies inside the cell. They are described as having both constitutive function (the ability to make proteins) and, crucially, the capacity to replicate, which distinguishes them from traditional, non-replicating mRNA vaccines. - It is explained that replication introduces additional mutation and recombination opportunities, because the RNA genome is copied more than once, and the process can produce variants that differ from the original design. - Central dogma exceptions and viral biology - The speakers explain that while the central dogma (DNA → RNA → protein) generally governs biology, some viruses violate this, with RNA viruses that replicate via RNA-dependent replication and even some reverse-transcribing retroviruses that convert RNA to DNA and integrate into genomes. This context is used to frame why replicon vaccines could behave unpredictably. - Potential risks of replication and spread - A core concern is that the replicon approach might allow the vaccine genome to spread beyond the initial target cells, potentially reaching other cells and tissues, or even spreading to other people via exosomes or other means. Exosomes can transport DNA, RNA, and proteins between cells; thus, the replicon genome could in theory be disseminated. - The possibility of homologous or heterologous recombination between replicon genomes and wild-type viruses could yield new variants. The panel emphasizes the difficulty of controlling such recombination in a living system. - Specific material and design considerations - The use of viral components like spike protein genes in replicon vaccines raises concerns about how these proteins might mutate or recombine during replication, potentially altering antigen presentation or safety. - A concern is raised about the lack of repair mechanisms in RNA replication (as opposed to DNA replication), which could make error rates higher and lead to unpredictable changes. - The panel notes that current replicon vaccine designs (including those using alphavirus backbones) inherently carry high mutation and recombination risk, and that the replicating systems may encounter unpredictable evolutionary dynamics inside the human body. - Safety signals and clinical anecdotes - The speakers cite cases of adverse events temporally associated with vaccines, including vascular inflammation and thrombosis, stroke-like events, and myocarditis, to illustrate that immune responses to vaccines can be complex and occasionally severe. They emphasize that such observations do not establish causality, but argue they warrant careful scrutiny. - There are references to cases of acute vascular and neural complications following repeated vaccination, and to broader immune dysregulation phenomena, including IGG4-related disease and immune dysregulation syndromes that can involve multiple organs. - One example concerns a patient who developed sudden limb problems after the third dose, requiring surgery; another describes myocardial involvement after multiple doses and subsequent inflammatory sequelae. - DNA contamination and analytical findings - Kevin McKernan’s analysis of certain Japanese CoronaVac vaccines is cited: both DNA contamination and the presence of SV40 promoter elements were detected in some vaccine lots, with DNA amounts exceeding some regulatory benchmarks in at least one case. The concern is that DNA contamination, or the presence of promoter sequences, could influence integration or expression in unintended ways. - It is noted that vaccines using lipid nanoparticles can potentially deliver nucleic acids into cells; in the presence of exons or promoter sequences, there could be unintended cellular uptake and expression. - Implications for public health and policy - The panel underscores the need for caution, thorough investigation, and long-term observation of any replication-based vaccine platform before broad deployment. There is a call to evaluate risks, monitor long-term outcomes, and consider the possibility that replication-competent constructs could drive unforeseen evolutionary dynamics within hosts or communities. - There is contention about how information is communicated to the public, with particular emphasis on avoiding misinformation while ensuring that scientific uncertainties are transparently discussed. - Broader scientific context and forward-looking stance - The speakers discuss how the field’s approach to gene-based vaccines is evolving rapidly, and they stress that the compatibility of replicon systems with human biology is not yet fully understood. - They frame their discussion as not merely about current vaccines but about the trajectory of vaccine platforms: if replication-based or self-dispersing systems prove too risky or unpredictable, the prudent path might be to favor conventional, non-replicating strategies until safety, efficacy, and containment of unintended spread are more firmly established. Closing and takeaways - The session closes with emphasis on careful evaluation of replicon vaccines, awareness that viral genetics can behave differently in humans than in theory, and a call for continued discussion, independent verification, and transparent communication as the technology develops. - Throughout, speakers acknowledge the complexity of immune responses to vaccines, the potential for unexpected adverse events, and the importance of safeguarding public health while advancing vaccine science.

Pfizer and Moderna vaccines use two processes. The first process involves using PCR to amplify and create DNA for clinical trials. Once approved, they use circular bacterial DNA plasmid to replicate billions of mRNA DNA sample copies. However, this resulted in contaminated vaccines with junk DNA. A study found DNA fragments in Pfizer and Moderna vaccines in Ontario, Canada. Researchers tested 27 mRNA vials from 12 different lots and discovered billions to 100 billions of DNA molecules per dose, exceeding FDA and WHO guidelines by 188 to 509 times. This is a significant amount, far beyond what is acceptable.

The Pfizer vaccine is contaminated with plasma DNA, not just mRNA. This DNA is the DNA vector used as the template for the in vitro transcription reaction. This was discovered by sequencing vials of Pfizer vaccine from Colombia. It's surprising that there's any DNA in there. The speaker is alarmed about the possible consequences of this, including rare but serious side effects like death from cardiac arrest. Mixing DNA with a lipid complex allows it to enter cells and become a permanent fixture. This is a real hazard for genome modification of long-lived somatic cells, like stem cells, and could cause a sustained autoimmune attack. There is also a very real theoretical risk of future cancer in some people. The risk is not zero and it may be high enough that we ought to figure out if this is happening or not.

The Pfizer vaccine contains DNA contamination in addition to mRNA. The DNA comes from the DNA vector used as a template for making the mRNA. Sequencing analysis of the vaccine revealed the presence of DNA, which could potentially cause serious side effects and integrate into the genomic DNA of cells. This poses risks such as autoimmune attacks and potential future cancer. The DNA contamination likely occurred during the production process. It is important to investigate if this DNA has integrated into the genomes of vaccinated individuals. The FDA should require Pfizer to remove the DNA from future versions of the vaccine. The regulatory limit for DNA in vaccines is outdated and not suitable for this type of vaccine. It is necessary to address this oversight and ensure the safety of the vaccine.