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We received four Pfizer vaccines, and the one we sequenced is different from any previously analyzed. This may be an earlier version that Pfizer modified later. The vaccine contains a common plasmid used in coronavirus research, which raises questions about its origin. While Pfizer is the likely source, it's possible it leaked from a lab. We found similarities and differences in the components of the vaccines. The contamination hypothesis is unlikely since the plasmids have never been handled in our labs, and formalin fixation prevents spike protein production. Pfizer could have multiple undisclosed plasmids, which would be concerning, especially since we found one in a colon cancer sample that produces spike protein at high levels, indicating it could be replication competent and potentially transmissible. Transmissible cancer is a significant issue.

Quan et al demonstrated that the introduction of DNA into a cell, even without integration, can trigger the oncogenic cGAS-STING pathway. The speaker claims that the presence of an SV40 origin of replication, a mammalian origin, in a vaccine grown in E. coli is reckless because it allows the plasmid DNA to replicate episomally in the host. The speaker alleges evidence suggests Pfizer, unlike Moderna, may have included this origin of replication due to carelessness. The speaker highlights concerns about nucleic acid persistence, noting that RT-PCR methods used in studies like Krausson and Rolchen may have amplified both DNA and RNA. The speaker suggests that prior studies assumed detected nucleic acids were RNA, but that further investigation using primers specific to the plasmid backbone might reveal the presence of residual plasmid DNA. The Krausson paper found nucleic acids present for thirty days in heart tissues, and the Rolchen paper found them for sixty days.

SV40 promoter sequences are still detectable 48 hours later, despite being wrapped in LNPs and using methods aimed at eliminating them. This raises urgent concerns about how long this DNA persists without DNA depletion protocols. The purification methods used to capture this DNA require thorough examination, as techniques like ethanol precipitation and mini DNA purification kits may not effectively eliminate small DNA fragments or could further degrade them.

Moderna holds a patent for using RNA in vaccines, acknowledging that RNA is superior to DNA due to concerns about DNA-related problems like insertional immunogenesis and genotoxicity. The FDA claims to be unaware of any issues, but Moderna's own patent raises the same concerns about DNA. It appears that DNA is present in the RNA preparation as a contaminant, as it is used in the process of making RNA. Recent findings by scientists revealed large numbers of DNA fragments in the RNA preparation, including sequences that are not normally allowed in human use, such as an antibiotic resistance gene and sequences from simian virus 40. These DNA fragments can potentially lead to DNA damage, birth defects, and cancer.

Moderna holds a patent for using RNA in vaccines, acknowledging that RNA is better than DNA due to concerns about DNA-related problems like insertional immunogenesis and genotoxicity. The FDA claims to be unaware of these concerns, but Moderna's own patent highlights them. The presence of DNA in the vaccines is considered a contaminant, as it is used in the process of making RNA. Recent findings by scientists in the US and Canada revealed large amounts of DNA fragments in the RNA preparation, including sequences not allowed for human use, such as an antibiotic resistance gene and sequences from simian virus 40. These DNA fragments pose risks of DNA damage, including birth defects and cancer.

Many labs, including Medicinal Genomics, found DNA contamination in Pfizer and Moderna mRNA vaccines. Regulators like the FDA and EMA admitted to this, but downplayed its significance. The SP 40 sequences omitted by Pfizer are crucial. DNA contamination can cause insertional mutagenesis, as stated in Moderna's patents. Regulatory agencies were deceived and failed to properly address the issue. This poses a serious risk that cannot be ignored.

The speaker claims sequences found in vaccines are now also being found in people. According to the speaker, five peer-reviewed studies involving RNA sequencing of vaccinated and unvaccinated individuals show DNA from vaccine manufacturers in patients' blood. The speaker cites studies by Ryan et al., Chakrabarty, Rhine, and Odek as evidence of Moderna and Pfizer vaccine sequences in patients' blood, suggesting blood supplies are contaminated. The speaker mentions three additional papers (Lee, Navel, and Krauszik) that, while not explicitly looking for it, also confirm residual plasmid sequences in recipients. One study, which investigated a particular disease, revealed differential gene expression in the cGAS-STING and interferon pathways, indicating a potential DNA stimulatory response. The speaker suggests this RNA sequencing data reveals the nature of the contaminant, with gene expression changes indicating a cGAS-STING-like event potentially induced by the DNA.

- The mRNA on plasmids was produced, and after processing, much DNA from plasmids remained; Kevin MacKinnon found that vials were full of plasmid DNA, the whole plasmid and parts of it, and this was published. Authorities claimed it doesn’t matter and that vaccines have saved millions of lives, so why not have some DNA in them. - The DNA in the vaccine vials was packaged in lipid nanoparticles and was shown by colleagues last year (the INMODIA publication) to enter human cells in culture and remain stable in cells for days, as did the mRNA. Despite this, the message given was to poof, never mind, don’t worry, be happy. - A radical change occurred due to a discovery by Kevin MacKinnon three weeks ago: during transcription on the chromosome, byproducts are generated; some mRNA strands do not detach from the DNA where they’re formed, creating hybrids of DNA and RNA that come off together. These hybrids are dangerous. - In cells, an enzyme called RNase H takes care of these sparks and extinguishes them immediately; otherwise they can cause damage to the chromosome, potentially lighting “fires” on the chromosomes. If not extinguished, the fires can cause diverse damage depending on where they occur, potentially leading to illnesses described in medical textbooks, including tumors (neoplastic disease), autoimmune disease, developmental impairment, birth defects, or death. - The speaker asserts these hybrids and their mishandling could lead to a broad range of illnesses, and emphasizes that this situation is not limited to the COVID vaccine but applies to all Moderna RNA vaccines, including new Moderna RNA vaccines entering the market, such as a flu vaccine, and mentions veterinary RNA vaccines as well. - The claim is made that these vaccines will be heavily contaminated with deadly dangerous hybrids, and it is the duty of authorities and controlling authorities to stop proceeding and not turn away; otherwise they will face court for not fulfilling their duties. The speaker has been giving interviews and asserts this narrative is spreading worldwide, framing it as akin to attempted murder and urging physicians to refuse vaccination.

Kevin McKernan, an experienced genomic expert, discusses his findings regarding mRNA vaccines. He discovered that the expression vectors for the vaccines, which are used to produce the spike protein, are still present in the vials. These vectors exceed the guidelines set by the EMA and FDA. McKernan explains the two different processes used in the clinical trials and how residual plasmids from E. coli amplification remain in the vaccines. He specifically highlights the presence of the SV40 promoter in Pfizer vaccines, which was not disclosed to the regulatory agencies. McKernan's team conducted quantitative PCR assays and found high levels of vector DNA in the vials. He emphasizes the importance of understanding the potential risks associated with this contamination and encourages others to replicate his work.

Key points: "the vials that were in fact approved are not the vials that were given to the public." The clinical trial used "process one that used PCR to make the DNA that was going to then turn into the RNA to make the spike protein." After the trial, "they switched" to a production process that "manufactured this DNA in E. Coli," introducing endotoxin risk. "There are these plasmids that have additional DNA that were not present in the actual clinical trial." Sequencing found mixtures, including "expired" and samples that had "been tapped into." Regarding Pfizer, "the Pfizer vaccines actually had a component that was not disclosed to the regulators." "The plasmid map on the right is what was disclosed to the EMA" with "no mention of the SV40 components" now "inside this DNA sequence." "The plasma on the left is what we actually found," with components not disclosed to regulators nor to patients. Monovalent Pfizer; prior ones were the bivalent vaccines from Moderna and Pfizer.

The speaker discusses the finding of plasmids in Pfizer vaccines, referencing a widely read paper that initiated inquiry into the consequences of this residual contaminant. David Speaker replicated this work in Canada, finding it in every one of over 30 vials. Philip Buchholz replicated this in South Carolina, and Dr. Sid Lee replicated the work using different primers and Sanger sequencing. Bridget Koning has replicated this in Germany, and several federal agencies have admitted the presence of DNA, though disagreeing on clinical implications. Ulrich Kammerer's lab replicated the work, transfecting plasmids from the vaccines into cell lines, where they persisted for several cycles of cell division. High schoolers interning at the FDA White Oak facility also measured it and found it to be significantly over the limit. Numerous studies have replicated these findings, with most finding levels over the limit. One individual with ties to Moderna claims it is not over the limit. Kaiser et al. claimed to have found it slightly over the limit but deemed it inconsequential; however, their DNA isolation method has been refuted by Kommer and Konig.

Pfizer's use of the RiboGreen technique to measure DNA in their vaccines has raised concerns about deceptive practices. The presence of billions of DNA fragments in each dose, some of which are small and more likely to integrate into the genome, is worrying. Preliminary data suggests a correlation between adverse events and contaminated Pfizer vaccines, but more research is needed. The DNA in the vaccines is different from previous contamination and carries a higher risk of integration. The FDA acknowledges the integration risk and the need for lower limits on DNA when copy numbers are high. The DNA is encapsulated in lipid nanoparticles, making it prothrombotic and potentially oncogenic. The presence of endotoxin and the spike protein in the vaccines further complicates the situation. The vaccines have been found in various tissues and can lead to prolonged expression of the spike protein. Insertional mutagenesis and cancer risk are concerns, especially for individuals with weakened immune systems. Regulatory bodies have confirmed the presence of the SV40 sequence in the vaccines, but the clinical implications are still unclear.

"Pfizer vaccine is contaminated with plasma DNA. It's not just mRNA." "This DNA is the DNA vector that was used as the template for the in vitro transcription reaction when they made the mRNA." "I sequenced it in my own lab." "The vials of Pfizer vaccine that were given out here in Colombia, one of my colleagues was in charge of that vaccination program in the College of Pharmacy." "And for reasons that I still don't understand, he kept every single vial." "So he had a whole freezer full of the empty vials." "And I checked these two batches, and I checked them by sequencing." "It's surprising that there's any DNA in there." "This DNA, in my view, it could be causing some of the rare but serious side effects like death from cardiac arrest."

We were surprised by the findings from a patient vaccinated four times with Pfizer. A year post-vaccination, tumors developed, and the patient died within a month. Biopsies revealed SV40 in the vaccine's origin of replication. Preliminary sequencing confirmed the presence of the Pfizer vaccine's DNA, including sequences from Spike protein. Unexpectedly, the DNA copy number in the tumors exceeded that of the human genome, indicating a significant insertional mutagenesis event. Instead of the anticipated lower mutation rates, our PCR results showed signals as strong as the original vaccine vial, suggesting the DNA is replicating within the patient. This indicates that the mammalian origin of replication in Pfizer's vaccine is active in human tumors.

A colon biopsy from a Pfizer-vaccinated individual who died a month after tumor emergence revealed SV40 in the origin of replication from the Pfizer vaccine. Preliminary sequencing suggests the presence of Pfizer's vaccine. The vaccine DNA copy number was greater than the human genome, indicating replication. PCR signals suggest the vaccine concentration is similar to or higher than the original vial, despite dilution in the body. This indicates the mammalian origin of replication in Pfizer's vaccine is active in human tumors. This finding may explain shedding, which is not supposed to occur because the COVID vaccine is not intended to alter DNA. However, evidence suggests that the vaccine may be altering DNA, making it a VGBT (genome altering) product. This could mean that spike proteins are continually created, and shedding is real.

Health Canada initially claimed the Pfizer vaccine did not contain SV40, but later acknowledged its presence, stating it has no functional role. Despite this, they sought clarification from Pfizer about the residual fragments. When asked for information, Health Canada provided redacted documents, raising questions about transparency. A technique called fluorometry, which labels DNA with fluorescent markers, revealed that some RNA vaccines, particularly Moderna's, could contain up to 10 trillion copies of DNA fragments per dose. This is concerning because just 3 to 10 copies can facilitate the incorporation of SV40 DNA into a cell's nuclear genome, potentially leading to mutations associated with cancer.

A peer-reviewed paper confirms Pfizer's mRNA vaccine is contaminated with DNA and SV40 enhancers, considered dangerous. Pfizer used bacterial plasma DNA during mass production, leading to contamination with spike protein genes, antibiotic resistance markers, and SV40 enhancers. Researchers found 4-5 times more DNA than the safe limit. The DNA fragments could integrate into the human genome due to the SV40 enhancer. Injected vaccine samples caused super strong kidney cells to produce spike protein, excreted via exosomes, potentially spreading body-wide. The vaccines were toxic to these cells, causing pathological changes. The DNA, tucked into lipid nanoparticles, could integrate into the human genome, turning the jab into accidental gene therapy. The SV40 enhancer drags DNA into the nucleus. The study suggests the inclusion of SV40 enhancers was deliberate, not accidental, and scrubbed from regulatory paperwork. Experts theorize this contamination could be linked to an explosion of turbo cancers. The study concludes mRNA shots should be suspended until safety is determined. Another study found vaccine spike protein expressed in cerebral arteries of stroke patients for up to 17 months, accompanied by an autoimmune response.

We have sequenced samples from a colon biopsy of a patient who was vaccinated four times and developed colon cancer a year later. The sequencing revealed plasmids, with about 100 copies per cell, which differ from Pfizer's. This raises questions about potential variations in manufacturing or contamination. Preliminary data suggests these plasmids may integrate into the genome, with one integration observed on chromosome 21 affecting a cancer-related gene. The high copy number indicates replication, as the expected dilution from vaccination would not account for such levels. The formalin-fixed tissue confirms these plasmids were present while the patient was alive, but the source remains unknown.

A peer-reviewed paper confirms Pfizer's mRNA vaccine is contaminated with dangerous DNA and SV40 enhancers. During production, bacterial plasmid DNA led to contamination with spike protein genes, antibiotic resistance markers, and SV40 enhancers. Researchers found four to five times more DNA than the safe limit; these DNA fragments could integrate into the human genome due to the SV40 enhancer. Experiments showed the vaccines were toxic to cells, causing pathological changes and spike protein production, potentially spreading body-wide via exosomes. The DNA contamination, tucked into lipid nanoparticles, could lead to unintended gene therapy. The SV40 enhancer, deliberately added but removed from regulatory paperwork, raises questions about Pfizer's intentions. Experts theorize this contamination could be linked to an increase in "turbo cancers," coinciding with Pfizer's acquisition of a cancer drugmaker. An epidemiologist noted the study found DNA from the manufacturing process way over regulatory limits, including the cancer-promoting SV40 promoter enhancer and spike-producing DNA. A separate study found vaccine spike protein expressed in cerebral arteries of stroke patients for up to seventeen months, potentially contributing to autoimmune responses. There are now 11 independent reports that have found, DNA contamination within these shots of up to 60000% above regulatory limits.

There is significant DNA contamination found in vaccines, with evidence from multiple researchers in Germany, Japan, and the U.S. Regulatory bodies like the FDA and EMA acknowledge this contamination but downplay its significance, relying on Pfizer's assurances. The clinical trials used cleaner DNA, but the mass-produced vaccines did not undergo the same purification, leading to increased background DNA and endotoxin levels. Regulators received a plasmid map missing crucial annotations, suggesting manipulation. Claims about expired vials and PCR methods used to measure contamination have been challenged, with evidence showing that Moderna's vaccines are cleaner. Regulators are allowing different measurement standards for RNA and DNA, raising concerns about transparency and integrity in the regulatory process.

The Pfizer vaccine contains not only mRNA but also plasma DNA from the vector used in its production. I sequenced samples from two batches of the vaccine in Colombia and found this DNA, which raises concerns about potential health risks. This DNA could integrate into the genomic DNA of cells, leading to permanent changes. Such integration poses theoretical risks, including autoimmune responses and cancer, depending on where the DNA inserts itself in the genome. While these risks may be rare, they warrant investigation to understand their implications better.

The panel discusses replication (replicon) vaccines and their potential dangers, focusing on how they differ from conventional messenger RNA (mRNA) vaccines and what new risks might emerge as this technology develops. Key points and concerns raised - Replicon vaccines concept and fundamental differences - Replicon vaccines use replication-capable genetic material, so the embedded genetic information not only makes antigen proteins but also multiplies inside the cell. They are described as having both constitutive function (the ability to make proteins) and, crucially, the capacity to replicate, which distinguishes them from traditional, non-replicating mRNA vaccines. - It is explained that replication introduces additional mutation and recombination opportunities, because the RNA genome is copied more than once, and the process can produce variants that differ from the original design. - Central dogma exceptions and viral biology - The speakers explain that while the central dogma (DNA → RNA → protein) generally governs biology, some viruses violate this, with RNA viruses that replicate via RNA-dependent replication and even some reverse-transcribing retroviruses that convert RNA to DNA and integrate into genomes. This context is used to frame why replicon vaccines could behave unpredictably. - Potential risks of replication and spread - A core concern is that the replicon approach might allow the vaccine genome to spread beyond the initial target cells, potentially reaching other cells and tissues, or even spreading to other people via exosomes or other means. Exosomes can transport DNA, RNA, and proteins between cells; thus, the replicon genome could in theory be disseminated. - The possibility of homologous or heterologous recombination between replicon genomes and wild-type viruses could yield new variants. The panel emphasizes the difficulty of controlling such recombination in a living system. - Specific material and design considerations - The use of viral components like spike protein genes in replicon vaccines raises concerns about how these proteins might mutate or recombine during replication, potentially altering antigen presentation or safety. - A concern is raised about the lack of repair mechanisms in RNA replication (as opposed to DNA replication), which could make error rates higher and lead to unpredictable changes. - The panel notes that current replicon vaccine designs (including those using alphavirus backbones) inherently carry high mutation and recombination risk, and that the replicating systems may encounter unpredictable evolutionary dynamics inside the human body. - Safety signals and clinical anecdotes - The speakers cite cases of adverse events temporally associated with vaccines, including vascular inflammation and thrombosis, stroke-like events, and myocarditis, to illustrate that immune responses to vaccines can be complex and occasionally severe. They emphasize that such observations do not establish causality, but argue they warrant careful scrutiny. - There are references to cases of acute vascular and neural complications following repeated vaccination, and to broader immune dysregulation phenomena, including IGG4-related disease and immune dysregulation syndromes that can involve multiple organs. - One example concerns a patient who developed sudden limb problems after the third dose, requiring surgery; another describes myocardial involvement after multiple doses and subsequent inflammatory sequelae. - DNA contamination and analytical findings - Kevin McKernan’s analysis of certain Japanese CoronaVac vaccines is cited: both DNA contamination and the presence of SV40 promoter elements were detected in some vaccine lots, with DNA amounts exceeding some regulatory benchmarks in at least one case. The concern is that DNA contamination, or the presence of promoter sequences, could influence integration or expression in unintended ways. - It is noted that vaccines using lipid nanoparticles can potentially deliver nucleic acids into cells; in the presence of exons or promoter sequences, there could be unintended cellular uptake and expression. - Implications for public health and policy - The panel underscores the need for caution, thorough investigation, and long-term observation of any replication-based vaccine platform before broad deployment. There is a call to evaluate risks, monitor long-term outcomes, and consider the possibility that replication-competent constructs could drive unforeseen evolutionary dynamics within hosts or communities. - There is contention about how information is communicated to the public, with particular emphasis on avoiding misinformation while ensuring that scientific uncertainties are transparently discussed. - Broader scientific context and forward-looking stance - The speakers discuss how the field’s approach to gene-based vaccines is evolving rapidly, and they stress that the compatibility of replicon systems with human biology is not yet fully understood. - They frame their discussion as not merely about current vaccines but about the trajectory of vaccine platforms: if replication-based or self-dispersing systems prove too risky or unpredictable, the prudent path might be to favor conventional, non-replicating strategies until safety, efficacy, and containment of unintended spread are more firmly established. Closing and takeaways - The session closes with emphasis on careful evaluation of replicon vaccines, awareness that viral genetics can behave differently in humans than in theory, and a call for continued discussion, independent verification, and transparent communication as the technology develops. - Throughout, speakers acknowledge the complexity of immune responses to vaccines, the potential for unexpected adverse events, and the importance of safeguarding public health while advancing vaccine science.

Pfizer and Moderna vaccines use two processes. The first process involves using PCR to amplify and create DNA for clinical trials. Once approved, they use circular bacterial DNA plasmid to replicate billions of mRNA DNA sample copies. However, this resulted in contaminated vaccines with junk DNA. A study found DNA fragments in Pfizer and Moderna vaccines in Ontario, Canada. Researchers tested 27 mRNA vials from 12 different lots and discovered billions to 100 billions of DNA molecules per dose, exceeding FDA and WHO guidelines by 188 to 509 times. This is a significant amount, far beyond what is acceptable.

The Pfizer vaccine contains DNA contamination in addition to mRNA. The DNA comes from the DNA vector used as a template for making the mRNA. Sequencing analysis of the vaccine revealed the presence of DNA, which could potentially cause serious side effects and integrate into the genomic DNA of cells. This poses risks such as autoimmune attacks and potential future cancer. The DNA contamination likely occurred during the production process. It is important to investigate if this DNA has integrated into the genomes of vaccinated individuals. The FDA should require Pfizer to remove the DNA from future versions of the vaccine. The regulatory limit for DNA in vaccines is outdated and not suitable for this type of vaccine. It is necessary to address this oversight and ensure the safety of the vaccine.

RNA sequencing of the Moderna vaccine's three prime ends suggests a possible mechanism for RNA persistence: in vivo re-adenylation. The data indicates plasmid DNA contamination despite efforts to reduce it. The data also reveals contamination from other mRNA vaccines in Moderna's pipeline. The speaker suggests that with widespread DNA sequencing capabilities, tolerating incorrect RNA sizes in vaccines is irresponsible. Sequencing before approval would have allowed for a better understanding of low RNA scores before global administration.