TruthArchive.ai - Tweets Saved By @CoyoteSanctuary

@CoyoteSanctuary — CoyoteSanctuary🐺🐭🦝🐿️😼🐾🐾

Posted - February 8, 2025 at 7:58 PM

Saved - February 9, 2025 at 11:21 AM

reSee.it AI Summary

I explored the mechanisms behind fibrinogen's autofluorescence, particularly its relevance to amyloid studies in neurodegenerative diseases. I noted the need for further research on amyloid specificity, especially using tools like Amytracker. I shared concerns about unusual arterial clots I've observed, linking them to the spike protein from COVID-19 and mRNA vaccines. The rising death rates and acknowledgment of strange clots by embalmers raise urgent questions. Collaborative research is ongoing, but it's not enough to address these critical issues.

@CoyoteSanctuary - CoyoteSanctuary🐺🐭🦝🐿️😼🐾🐾

Credit @NestCommander Why would fibrinogen be auto fluorescing , explain the mechanisms in necessary detail for our discussions on amyloid. 3/3 ChatGPT said: Fibrinogen exhibits autofluorescence under certain conditions due to its structural and biochemical properties. In the context of amyloid discussions, understanding fibrinogen autofluorescence is important because fibrinogen can be involved in amyloid formation and aggregation, particularly in neurodegenerative diseases and coagulopathies. Below are the key mechanisms explaining why fibrinogen might autofluoresce: 1. Intrinsic Fluorophores in Fibrinogen Fibrinogen contains aromatic amino acids that exhibit intrinsic fluorescence: Tryptophan (Trp) (~340 nm excitation, ~350 nm emission) Tyrosine (Tyr) (~275 nm excitation, ~303 nm emission) Phenylalanine (Phe) (~258 nm excitation, weak emission at ~282 nm) These residues contribute to fibrinogen’s natural fluorescence, with tryptophan being the dominant fluorophore. The fluorescence properties can change depending on fibrinogen’s structural state or interactions with other molecules. 2. Structural Perturbations and Amyloid Formation When fibrinogen undergoes misfolding or forms amyloid-like aggregates, its intrinsic fluorescence properties can be altered due to: Changes in aromatic residue microenvironments – As fibrinogen aggregates, tryptophan and tyrosine residues may be exposed or buried differently, leading to shifts in fluorescence emission. Enhanced beta-sheet content – Amyloid fibrils are rich in beta-sheet structures, which can alter the electronic environment of aromatic residues, modifying fluorescence characteristics. 3. Post-Translational Modifications Oxidation & Glycation – Oxidative stress or non-enzymatic glycation can introduce fluorescent adducts to fibrinogen. Advanced Glycation End-products (AGEs) – These accumulate in amyloid-associated conditions and exhibit strong autofluorescence in the 350–450 nm range upon UV excitation. 4. Interaction with Amyloid-Specific Dyes Amyloid dyes like Thioflavin T (ThT) or Congo Red (CR) bind to fibrinogen amyloid-like structures and exhibit fluorescence enhancement. This suggests that fibrinogen, when adopting amyloid-like conformations, may have similar autofluorescent properties. Relevance to Amyloid Studies Fibrinogen’s autofluorescence is particularly significant in: Neurodegeneration (e.g., Alzheimer’s Disease) – Fibrinogen interacts with amyloid-beta (Aβ) and contributes to fibrillar deposits. Hypercoagulability & Systemic Amyloidosis – Altered fibrin clot structures in amyloid-prone conditions may show enhanced fluorescence due to misfolding and aggregation. Thus, fibrinogen's autofluorescence is a useful property when studying its role in amyloid diseases, allowing for non-invasive detection and structural analysis of its pathological aggregates.

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Specificity not nailed down for amyloids. It may exist. = Research needed for existing studies using Amytracker 480 et al looking for specificity confirmed with SEM/EDX near 1700 frequency peaks ( Fibrinogen ) Amytracker ™ https://www.ebbabiotech.com/collections/amytracker

Amytracker Optotracers for labeling amyloids and other protein aggregates Amytracker allow high quality visualisation of protein aggregates and detect prefibrillar- and fibrillar states of amyoids originating from a variety of amyloidogenic proteins or peptides. Amytracker can be used in tissue sections, live cells and can be app ebbabiotech.com

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Excitation and Emission ranges Match With a minimum of 8 alpha beta sheets aggregated into an Amyloid Fibrilla form

@CoyoteSanctuary - CoyoteSanctuary🐺🐭🦝🐿️😼🐾🐾

The End Result. And why time is so critical.

@r_hirschman - Richard Hirschman

Today is February 7th, here's a few pictures from this month of the strange clots that I have been seeing since early 2021. I believe it is related to the spike protein, either from the virus or the mRNA VACCINE which makes your body create the spike protein which causes the body create abnormal clots. You might think it is from chemicals in our foods or bad diet, maybe even toxins sprayed from the sky! Whatever is causing it, we need to figure it out! I had 3 bodies in one day this week that had clots in the arteries! According to a Google search. 25% of the population may have arterial thrombosis, that's like 1 in 400! How Is it statistically possible that I see it in 3 corpses in one day? According to Tom Haviland and his latest survey of Embalmers, 83% of them are acknowledging the strange fibrous clots! When will this end? When enough people demand answers. Is this why so many politicians are against Robert F Kennedy Jr? 🤔 Remember who pushed this experimental mRNA vaccine platform with mandates for you but exemptions for themselves. Where were the politicians defending your freedom from experimental vaccine mandates!

@CoyoteSanctuary - CoyoteSanctuary🐺🐭🦝🐿️😼🐾🐾

@HouseLyndseyRN - Lyndsey, RN 💜🐭

Arterial Blood clots YALL MAKE IT STOP NOW

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@r_hirschman - Richard Hirschman

@aerynjurfgh

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@CharlesRixey - Charles Rixey, MA MBA (c) 🐭

Deaths in Western Australia were 20% higher in 2024 than in 2020 - and rising. Yet USAID - I mean, the media - is baffled and silent

@CoyoteSanctuary - CoyoteSanctuary🐺🐭🦝🐿️😼🐾🐾

@UveBeenNudged_ - UveBeenNudged memes

@NestCommander @CharlesRixey @BillyBostickson @still_a_nerd @Doctor_I_am_The @pizzapicklespur @Jikkyleaks @NameIsSpartacus

@CoyoteSanctuary - CoyoteSanctuary🐺🐭🦝🐿️😼🐾🐾

Live Embalmer Clot Analysis: 🦝🔦 https://rumble.com/v6gyzq4-vaccine-injuredlong-covid-clots-and-a-first-look-at-cadaver-calamari-clots.html

@CoyoteSanctuary - CoyoteSanctuary🐺🐭🦝🐿️😼🐾🐾

Discussions of experiments performed this morning, some running through Monday morning. Spike Protein S1-SUBUNIT reactions with human blood drawn on stream, looking for reactions with Thivoflavin T, RT-QUIC + PBS solution Discussions: Replication of Professors Kell, Pretorius

Video Transcript AI Summary

Our study investigates the impact of the SARS-CoV-2 spike protein on blood hypercoagulation. We used microscopy and mass spectrometry to examine the spike protein's interaction with platelets and fibrinogen. Results using platelet-poor plasma showed the spike protein may disrupt blood flow. Mass spectrometry revealed structural changes in beta and gamma fibrinogen, complement, and prothrombin after adding spike protein S1. These changes, similar to those observed in COVID-19 patient blood clots, showed resistance to trypsinization. We propose that the spike protein's presence in the bloodstream contributes to hypercoagulation in COVID-19 patients, potentially causing impaired fibrinolysis and persistent microclots. This finding has significant clinical implications. Our goal was to determine the spike protein's effects, as its interaction with these blood components warrants further investigation.

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Speaker 0: Yes. It's good. Okay. So we'll just go through that abstract real quick. So SARS CoV-two induced infection that cause coronavirus is characterized by unprecedented clinical pathologies. The most important pathologies is hypercoagulation of microclots in the lungs of patients. Here we study the effect of isolated SARS CoV-two spike protein subunit as potential in flammagene suiogenesis. Using scanning electron and fluorescent microscopy as well as mass spectrometry, we investigate the potential of this flammagene to interact with platelets and fibrinogen directly to cause blood hypercoagulation. Using platelet poor plasma, we show that spike protein may interfere with blood flow. Mass spectrometry also showed that when spike protein S1 is added to healthy platelet poor plasma, it results in structural changes to beta and gamma fibrinogen, complement free and prothrombin. And that kind of, lines up with the data that has been retrieved with mass spec on the calamari clots. It's beta. So in the calamari clots, it's beta fibrinogen, which, seems to be in excess when, as I understand it, they should basically all be in sort of like a one:one ratio. So it results in structural changes to beta and gamma fibrinogen, complementary and prothrombin. These proteins were substantially resistant to trypsinization and the presence of spike protein S1. Here, we suggest that in part, the presence of spike protein in circulation may contribute to hypercoagulation in in COVID-nineteen positive patients and may cause substantial impairment of fibrinolysis. Such lytic impairment may result in persistent large microclots we have noted here and previously in plasma samples of COVID-nineteen patients. It's observation that may have important clinical relevance. And so, what I'm interested in because I want to know if this spike protein that we have is doing anything, right? Because this should be, if we look at their methods,

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Continuing 2/4

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We purchased spike protein subunit MFC tag from Sino Biological and prepared it using doubly distilled water. A stock solution (0.25 mg/ml) was created by adding 400 µl of diluent to 100 µg of spike protein. This was diluted to working solutions. We assessed different spike protein concentrations in platelet-poor plasma using fluorescence microscopy. A healthy blood sample was divided into four tubes with varying spike protein concentrations (1000, 100, 50, and 1 ng/ml). Samples were incubated for 30 minutes at room temperature. We're replicating this experiment. I'll extract blood, add PBS buffer, and the spike protein. Then we'll look at the fluorescence.

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Speaker 0: If we look at their methods, aerials and methods. Put some collection. Spike protein. So spike protein subunit MFC tag was purchased from Sino Biological and prepared using doubly distilled water following the instructions. Four hundred microliter diluent endotoxin free water was added to one hundred microgram spike to create a stock solution of zero point two five mgs per ml. This stock solution was diluted to working solutions, determining the concentration of spike protein needed to result in significant but yet high enough concentration to cause physiological effects in viscoelasticity properties of blood, different concentrations of spike protein in platelet poor plasma assessed, fluorescence microscopy. Healthy controlled blood sample was separated into four one point five ml Eppendorf tubes with different final exposure concentrations of spike protein, 1,000 nanograms 100 per ml, which is what we're using, fifty nanograms per ml, ten nanograms per ml and one nanogram per ml. Bladder port samples were incubated with various spike concentrations for thirty minutes at room temperature, All right? So we're basically gonna just try and replicate that experiment right now with the spike protein that we have here. And we're gonna spin down my blood and, we'll add the buffer solution. So, in this case, the PBS that I used to make up the, the reactants for the RT Quick. And, of course, this spike protein. And, yeah, I suppose that means I've gotta jam myself with a needle right now. So I need to find, blood extraction materials. So just, just bear with me. Here, you can look at the fluorescence whilst we're here. If someone could post the link to this, cow and, praetorian.

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3/4

Video Transcript AI Summary

Okay, let's get started. I need to find the right tools to draw blood, so please be patient. I'll put the scope back on so we can watch. Here are some micrographs: healthy predlopod plasma, then the same plasma with spike protein added. We want to see if adding spike protein directly to healthy blood creates larger microclots than we see in the samples with the spike protein already present. We'll compare the images to see the effects.

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Speaker 0: Thank you. Where is the chat? Speaker 1: Main voice channel. There it Speaker 0: that should be in the chat. Alright. I gotta I gotta fish around for, stuff to pull blood, so just bear with me. I'll put the scope back on. Yeah. You see the scope? Yeah. We'll watch the border. So oh, no. Before, let's just go down and, Alright. So figure four. Representative fluorescent micrographs of predlopod plasma from healthy individuals after addition of fireflavine. PPP smear, PPP smear with spike protein. C is with from been added. We can't do that. And, PPP exposed to spike protein. So, basically, what I would like to do, is, Speaker 1: add just see if Speaker 0: we can get much bigger microclots than we do with my healthy blood with directly adding spike protein. Okay? Speaker 1: And that's that's the image. Oh, you don't see that image, but

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4/4 End Discussions Before Blood Draw of Doctor McCairn 🦝 PDF download link for Professors Kell and Pretorius manuscript being replicated : https://www.medrxiv.org/content/10.1101/2021.03.05.21252960v1

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Here's a shorter version of the transcript: We're examining fluorescent micrographs of plasma from healthy individuals. We're looking at a PPP smear, a smear with added spike protein, and plasma exposed to spike protein. The goal is to see if adding spike protein creates larger microclots than in healthy blood. We'll be conducting an experiment to investigate this. A question was raised about whether blood type matters, specifically if O positive individuals have fewer reactions to COVID. While I'm not certain, it's something to consider.

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Speaker 0: I'll put the scope back on. Yeah. You see the scope? Watch the border. So oh, now before, let's just go down and, Alright. So figure four. Representative fluorescent micrographs of plasma from healthy individuals of tradition of fireflavine. PPP smear, PPP smear with spike protein. C is with from been added. We can't do that. And, PPP exposed to spike protein. So, basically, what I would like to do, is add to see if we can get much bigger microclots than we do with my healthy blood with directly adding spike protein. Okay? And that's that's the image. Oh, you don't see that image, but That right. So this we're gonna do for experiment. Speaker 1: Kev, do you also think it matters the type of blood? So say if you're, o positive, I believe that they had the least amount of reactions to to COVID. Speaker 0: I I'm sure they're

SARS-CoV-2 spike protein S1 induces fibrin(ogen) resistant to fibrinolysis: Implications for microclot formation in COVID-19 medRxiv - The Preprint Server for Health Sciences medrxiv.org

@CoyoteSanctuary - CoyoteSanctuary🐺🐭🦝🐿️😼🐾🐾

More Video clips to be uploaded of Lab work+ Fluorescent microscopy & RT-QUIC as four more hours of recordings are reviewed What we're looking at becomes these Embalmer clots. By analyzing their composition+determination of how they form, We go forward ⚕️ https://t.co/YnEBSmThIB

@r_hirschman - Richard Hirschman

Thrombosis is a blood clot. Embolus or embolism is a foreign object that is moving in the vascular system, such as a piece of a blood clot that breaks free and is no longer stationary. Does this explain the strange fibrous clots that I have been seeing since early 2021? My guess is YES! Why? Because the spike protein causes abnormal clots and therefore the virus itself can cause clotting, but the mRNA vaccine makes your body create the spike protein that causes abnormal clotting. Do these clots look normal to you? Blood clots should be red! Only red!

@CoyoteSanctuary - CoyoteSanctuary🐺🐭🦝🐿️😼🐾🐾

https://t.wtyl.live/w/mPLvAmHnUKtbcRQkSVNCx4 The Prion Project

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https://t.co/X9moY0CoWo

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https://t.co/H9gyF3hDZQ

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https://t.co/ntiXLhDhmh

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https://t.co/EwfCeFm6td

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Thankfully Parallel research and collaboration IS Occurring Albeit Not nearly enough. https://t.co/RpyJ01tBEL

@Greg21143362 - Greg

@Kevin_McKernan Meanwhile, others keep digging to identify the remaining ‘elephant in the room’… https://t.co/7A1FJQ0trU

@CoyoteSanctuary — CoyoteSanctuary🐺🐭🦝🐿️😼🐾🐾

Posted - December 20, 2024 at 6:39 AM

Saved - December 20, 2024 at 6:54 AM

reSee.it AI Summary

I found the discussion intriguing, especially regarding the contents of vaccine vials and human blood samples. There's a focus on the need for advanced analysis, specifically using a Thermo Fischer Raman Spectrometer, which costs $30,000. If we were to analyze the chemical compounds and structures of amyloids in blood and vaccine particles, it would only cost $300 per person for 100 people. Understanding these elements could lead to effective mitigation methods.

@CoyoteSanctuary - CoyoteSanctuary🐺🐭🦝🐿️😼🐾🐾

That was interesting 🤔

@CoyoteSanctuary - CoyoteSanctuary🐺🐭🦝🐿️😼🐾🐾

https://t.co/dIOLsw5Auv

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https://t.co/l3Zch0A8B6

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https://t.co/9TJaF5mpIk

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https://t.co/OES4ASjFQk

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https://t.co/370Dkcm2kO

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https://t.co/P2eRORLuA9

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Why it's needed: This is in the Vaccine Vials https://t.co/6Zv7QRf803

@CoyoteSanctuary - CoyoteSanctuary🐺🐭🦝🐿️😼🐾🐾

This is in a human blood sample, not a sample that's been observed on video before (live tonight) https://t.co/lZ9jIl2Mnk

@CoyoteSanctuary - CoyoteSanctuary🐺🐭🦝🐿️😼🐾🐾

The Cost for a Thermo Fischer Raman Spectrometer Is 30 k. For 100 people on the planet That's 300.00 each to analyze the chemical compounds and structure of the Amyloid found in the blood and the particles found in the Vaccines. Once that's known Mitigation methods can 💡