TruthArchive.ai - Tweets Saved By @StevePascolo

@StevePascolo - Prof. Dr. Steve Pascolo

Le moyen fiable de quantifier l’ADN dans l’ARN est la spectrométrie de masse qui permet très spécifiquement de quantifier les riboses (ARN) et les deoxyriboses (ADN). Avec cette méthode: les vaccins ARNm contiennent bien 1000 fois moins d’ADN que d’ARNm (moins de 30 ng d’ADN par injection) comme stipulé dans les spécifications. Les incompétents (e.g. @raoult_didier) qui ont quantifié l’ADN avec le Qubit et obtenu des quantités énormes ne savent juste pas que Qubit est faussé par l’ARN et les lipides et que donc leurs résultats Qubit ADN sur les vaccins sont erronés. https://www.sciencedirect.com/science/article/pii/S0264410X25003196

Quantification of objective concentrations of DNA impurities in mRNA vaccines The COVID-19 pandemic has demonstrated the benefits and advantages of mRNA technologies in combination with lipid nanoparticle (LNP) delivery systems … sciencedirect.com

@ZatAwel - zat awel

@StevePascolo @russeurope Le monsieur qui se prétend le vrai scientifique en insultant ses collègues largement aussi compétents que lui, ça pue exactement comme le scientifique de plateau qui venait vomir pendant la période covid ses débilités quotidiennes.

@russeurope - Jacques Sapir

@ZatAwel @StevePascolo Sauf que lui est un spécialiste de la question de l'ARNm, avec une longue antériorité des travaux, et pas Raoult Donc, c'est nettement plus sérieux

@DjaonBea - ✨DJΛӨП BΣΛ✨ 🐿️ (🌿🌺🍀🌳)❤️CO2

@russeurope @ZatAwel @StevePascolo Vous devriez vous en tenir à l'économie...

@russeurope - Jacques Sapir

@DjaonBea @ZatAwel @StevePascolo Et vous, vous devriez vous taire

@StevePascolo - Prof. Dr. Steve Pascolo

Le moyen fiable de quantifier l’ADN dans l’ARN est la spectrométrie de masse qui permet très spécifiquement de quantifier les riboses (ARN) et les deoxyriboses (ADN). Avec cette méthode: les vaccins ARNm contiennent bien 1000 fois moins d’ADN que d’ARNm (moins de 30 ng d’ADN par injection) comme stipulé dans les spécifications. Les incompétents (e.g. @raoult_didier) qui ont quantifié l’ADN avec le Qubit et obtenu des quantités énormes ne savent juste pas que Qubit est faussé par l’ARN et les lipides et que donc leurs résultats Qubit ADN sur les vaccins sont erronés. https://www.sciencedirect.com/science/article/pii/S0264410X25003196

Quantification of objective concentrations of DNA impurities in mRNA vaccines The COVID-19 pandemic has demonstrated the benefits and advantages of mRNA technologies in combination with lipid nanoparticle (LNP) delivery systems … sciencedirect.com

@ZatAwel - zat awel

@StevePascolo @russeurope Le monsieur qui se prétend le vrai scientifique en insultant ses collègues largement aussi compétents que lui, ça pue exactement comme le scientifique de plateau qui venait vomir pendant la période covid ses débilités quotidiennes.

@russeurope - Jacques Sapir

@ZatAwel @StevePascolo Sauf que lui est un spécialiste de la question de l'ARNm, avec une longue antériorité des travaux, et pas Raoult Donc, c'est nettement plus sérieux

@DjaonBea - ✨DJΛӨП BΣΛ✨ 🐿️ (🌿🌺🍀🌳)❤️CO2

@russeurope @ZatAwel @StevePascolo Vous devriez vous en tenir à l'économie...

@russeurope - Jacques Sapir

@DjaonBea @ZatAwel @StevePascolo Et vous, vous devriez vous taire

@DjaonBea - ✨DJΛӨП BΣΛ✨ 🐿️ (🌿🌺🍀🌳)❤️CO2

@russeurope @ZatAwel @StevePascolo J'ai bien archivé pour la postérité vos réponses

@BanounHelene - Hélène Banoun

Merci de vous renseigner et de reproduire les manip faites pas ces équipes indépendantes qui retrouvent toutes la contamination ADN Avez-vous réalisé ces dosages? Kevin McKernan at the FDA :Food and Drug Administration (FDA), Center for Biologics Evaluation and Research (CBER), 182nd Meeting of the Vaccines and Related Biological Products Advisory Committee (VRBPAC), Zoom Video Conference, June 15, 2023 https://www.fda.gov/media/169804/download Kevin McKernan et al., 2023 Sequencing of bivalent Moderna and Pfizer mRNA vaccines reveals nanogram to microgram quantities of expression vector dsDNA per dose https://osf.io/preprints/osf/b9t7m_v1 Speicher et al :DNA fragments detected in monovalent and bivalent Pfizer/BioNTech and Moderna modRNA COVID-19 vaccines from Ontario, Canada: Exploratory dose response relationship with serious adverse eventsnts. https://osf.io/preprints/osf/mjc97_v1 David Speicher at the TGA : DNA contamination in mRNA vaccines, document 19, consulté le 3 avril 2025, https://www.tga.gov.au/sites/default/files/2024-12/FOI%2025-0070.pdf David Speicher (Ontario Veterinary College) at TGA : DNA contamination in mRNA vaccines, 9 septembre 2024, https://russellbroadbent.com.au/wp-content/uploads/David-Speicher-Report-2.pdf Pr Phillip Buckhaults, Professor of Cancer Molecular Genetics, University of South Carolina, at the South Carolina Senate, 16 octobre 2023, https://www.scstatehouse.gov/CommitteeInfo/SenateMedicalAffairsCommittee/PandemicPreparedness/PandemicPreparedness.phpconsulté le 3 avril 2025 https://www.scstatehouse.gov/CommitteeInfo/SenateMedicalAffairsCommittee/PandemicPreparedness/Phillip-Buckhaults-SC-Senate-09122023-final.pdf König, B.; Kirchner, J.O. Methodological Considerations Regarding the Quantification of DNA Impurities in the COVID-19 mRNA Vaccine Comirnaty®. Methods Protoc. 2024, 7, 41. https://doi.org/10.3390/mps7030041 Pr Didier Raoult : Didier Raoult, Confirmation of the presence of vaccine DNA in the Pfizer anti-COVID-19 vaccine 2024. ffhal-04778576f https://hal.science/hal-04778576v1/document Kämmerer U, Schulz V, Steger K. BioNTech RNA-Based COVID-19 Injections Contain Large Amounts Of Residual DNA Including An SV40 Promoter/Enhancer Sequence. Science, Public Health Policy and the Law. 2024 Dec 03; v5.2019-2024 https://publichealthpolicyjournal.com/biontech-rna-based-covid-19-injections-contain-large-amounts-of-residual-dna-including-an-sv40-promoter-enhancer-sequence/ Wang, Tyler J, Alex Kim, and Kevin Kim. 2024. “A Rapid Detection Method of Replication-Competent Plasmid DNA from COVID-19 mRNA Vaccines for Quality Control.” Journal of High School Science 8 (4): 427–39. https://jhss.scholasticahq.com/article/127890-a-rapid-detection-method-of-replication-competent-plasmid-dna-from-covid-19-mrna-vaccines-for-quality-control Sonia Pekova : Quantitative Multiplex Real-Time PCR analysis of Moderna (Spikevax) and Pfizer (BNT162b2) vaccines, Sona Pekova, MD, PhD., TILIA LABORATORIES s.r.o., Laboratory for molecular diagnostics, Pchery, Czech Republic 08.03.2025 https://www.10letters.org/CzechResearch.pdf

Sequencing of bivalent Moderna and Pfizer mRNA vaccines reveals nanogram to microgram quantities of expression vector dsDNA per dose Several methods were deployed to assess the nucleic acid composition of four vials of the Moderna and Pfizer bivalent mRNA vaccines. Two vials from each vendor were evaluated with Illumina sequencing, qPCR, RT-qPCR, Qubit™ 3 fluorometry and Agilent Tape Station™ electrophoresis. Multiple assays support DNA contamination that exceeds the European Medicines Agency (EMA) 330ng/mg requirement and the FDAs 10ng/dose requirements. These data may impact the surveillance of vaccine mRNA in breast milk or plasma as RT-qPCR assays targeting the vaccine mRNA cannot discern DNA from RNA without RNase or DNase nuclease treatments. Likewise, studies evaluating the reverse transcriptase activity of LINE-1 and vaccine mRNA will need to account for the high levels of DNA contamination in the vaccines. The exact ratio of linear fragmented DNA versus intact circular plasmid DNA is still being investigated. Quantitative PCR assays used to track the DNA contamination are described. osf.io

DNA fragments detected in monovalent and bivalent Pfizer/BioNTech and Moderna modRNA COVID-19 vaccines from Ontario, Canada: Exploratory dose response relationship with serious adverse events. Background: In vitro transcription (IVT) reactions used to generate nucleoside modified RNA (modRNA) for SARS-CoV-2 vaccines currently rely on an RNA polymerase transcribing from a DNA template. Production of modRNA used in the original Pfizer randomized clinical trial (RCT) utilized a PCR-generated DNA template (Process 1). To generate billions of vaccine doses, this DNA was cloned into a bacterial plasmid vector for amplification in Escherichia coli before linearization (Process 2), expanding the size and complexity of potential residual DNA and introducing sequences not present in the Process 1 template. It appears that Moderna used a similar plasmid-based process for both clinical trial and post-trial use vaccines. Recently, DNA sequencing studies have revealed this plasmid DNA at significant levels in both Pfizer-BioNTech and Moderna modRNA vaccines. These studies surveyed a limited number of lots and questions remain regarding the variance in residual DNA observed internationally. Methods: Using previously published primer and probe sequences, quantitative polymerase chain reaction (qPCR) and Qubit® fluorometry was performed on an additional 27 mRNA vials obtained in Canada and drawn from 12 unique lots (5 lots of Moderna child/adult monovalent, 1 lot of Moderna adult bivalent BA.4/5, 1 lot of Moderna child/adult bivalent BA.1, 1 lot of Moderna XBB.1.5 monovalent, 3 lots of Pfizer adult monovalent, and 1 lot of Pfizer adult bivalent BA.4/5). The Vaccine Adverse Events Reporting System (VAERS) database was queried for the number and categorization of adverse events (AEs) reported for each of the lots tested. The content of one previously studied vial of Pfizer COVID-19 vaccine was examined by Oxford Nanopore sequencing to determine the size distribution of DNA fragments. This sample was also used to determine if the residual DNA is packaged in the lipid nanoparticles (LNPs) and thus resistant to DNaseI or if the DNA resides outside of the LNP and is DNaseI labile.  Results: Quantification cycle (Cq) values (1:10 dilution) for the plasmid origin of replication (ori) and spike sequences ranged from 18.44 - 24.87 and 18.03 - 23.83 and for Pfizer, and 22.52 – 24.53 and 25.24 – 30.10 for Moderna, respectively. These values correspond to 0.28 – 4.27 ng/dose and 0.22 - 2.43 ng/dose (Pfizer), and 0.01 -0.34 ng/dose and 0.25 – 0.78 ng/dose (Moderna), for ori and spike respectively measured by qPCR, and 1,896 – 3,720 ng/dose and 3,270 – 5,100 ng/dose measured by Qubit® fluorometry for Pfizer and Moderna, respectfully. The SV40 promoter-enhancer-ori was only detected in Pfizer vials with Cq scores ranging from 16.64 – 22.59. In an exploratory analysis, we found preliminary evidence of a dose response relationship of the amount of DNA per dose and the frequency of serious adverse events (SAEs). This relationship was different for the Pfizer and Moderna products. Size distribution analysis found mean and maximum DNA fragment lengths of 214 base pairs (bp) and 3.5 kb, respectively. The plasmid DNA is likely inside the LNPs and is protected from nucleases. Conclusion: These data demonstrate the presence of billions to hundreds of billions of DNA molecules per dose in these vaccines. Using fluorometry, all vaccines exceed the guidelines for residual DNA set by FDA and WHO of 10 ng/dose by 188 – 509-fold. However, qPCR residual DNA content in all vaccines were below these guidelines emphasizing the importance of methodological clarity and consistency when interpreting quantitative guidelines. The preliminary evidence of a dose-response effect of residual DNA measured with qPCR and SAEs warrant confirmation and further investigation. Our findings extend existing concerns about vaccine safety and call into question the relevance of guidelines conceived before the introduction of efficient transfection using LNPs. With several obvious limitations, we urge that our work is replicated under forensic conditions and that guidelines be revised to account for highly efficient DNA transfection and cumulative dosing. osf.io

Page not found - Russell Broadbent MP russellbroadbent.com.au

South Carolina Legislature Online - Error scstatehouse.gov

South Carolina Legislature Online - Error scstatehouse.gov

BioNTech RNA-Based COVID-19 Injections Contain Large Amounts Of Residual DNA Including An SV40 Promoter/Enhancer Sequence - Science, Public Health Policy and the Law Background: BNT162b2 RNA-based COVID-19 injections are specified to transfect human cells to efficiently produce spike proteins for an immune response. publichealthpolicyjournal.com

A rapid detection method of replication-competent plasmid DNA from COVID-19 mRNA vaccines for quality control | Published in Journal of High School Science By Tyler J Wang, Alex Kim & 1 more. DNA contamination is the primary reason that undermines public trust in the quality of mRNA vaccines. We report a method to detect residual replication-competent plasmid DNA present in mRNA vaccines. jhss.scholasticahq.com

@StevePascolo - Prof. Dr. Steve Pascolo

Le moyen fiable de quantifier l’ADN dans l’ARN est la spectrométrie de masse qui permet très spécifiquement de quantifier les riboses (ARN) et les deoxyriboses (ADN). Avec cette méthode: les vaccins ARNm contiennent bien 1000 fois moins d’ADN que d’ARNm (moins de 30 ng d’ADN par injection) comme stipulé dans les spécifications. Les incompétents (e.g. @raoult_didier) qui ont quantifié l’ADN avec le Qubit et obtenu des quantités énormes ne savent juste pas que Qubit est faussé par l’ARN et les lipides et que donc leurs résultats Qubit ADN sur les vaccins sont erronés. https://www.sciencedirect.com/science/article/pii/S0264410X25003196

Quantification of objective concentrations of DNA impurities in mRNA vaccines The COVID-19 pandemic has demonstrated the benefits and advantages of mRNA technologies in combination with lipid nanoparticle (LNP) delivery systems … sciencedirect.com

@BanounHelene - Hélène Banoun

Merci de vous renseigner et de reproduire les manip faites pas ces équipes indépendantes qui retrouvent toutes la contamination ADN Avez-vous réalisé ces dosages? Kevin McKernan at the FDA :Food and Drug Administration (FDA), Center for Biologics Evaluation and Research (CBER), 182nd Meeting of the Vaccines and Related Biological Products Advisory Committee (VRBPAC), Zoom Video Conference, June 15, 2023 https://www.fda.gov/media/169804/download Kevin McKernan et al., 2023 Sequencing of bivalent Moderna and Pfizer mRNA vaccines reveals nanogram to microgram quantities of expression vector dsDNA per dose https://osf.io/preprints/osf/b9t7m_v1 Speicher et al :DNA fragments detected in monovalent and bivalent Pfizer/BioNTech and Moderna modRNA COVID-19 vaccines from Ontario, Canada: Exploratory dose response relationship with serious adverse eventsnts. https://osf.io/preprints/osf/mjc97_v1 David Speicher at the TGA : DNA contamination in mRNA vaccines, document 19, consulté le 3 avril 2025, https://www.tga.gov.au/sites/default/files/2024-12/FOI%2025-0070.pdf David Speicher (Ontario Veterinary College) at TGA : DNA contamination in mRNA vaccines, 9 septembre 2024, https://russellbroadbent.com.au/wp-content/uploads/David-Speicher-Report-2.pdf Pr Phillip Buckhaults, Professor of Cancer Molecular Genetics, University of South Carolina, at the South Carolina Senate, 16 octobre 2023, https://www.scstatehouse.gov/CommitteeInfo/SenateMedicalAffairsCommittee/PandemicPreparedness/PandemicPreparedness.phpconsulté le 3 avril 2025 https://www.scstatehouse.gov/CommitteeInfo/SenateMedicalAffairsCommittee/PandemicPreparedness/Phillip-Buckhaults-SC-Senate-09122023-final.pdf König, B.; Kirchner, J.O. Methodological Considerations Regarding the Quantification of DNA Impurities in the COVID-19 mRNA Vaccine Comirnaty®. Methods Protoc. 2024, 7, 41. https://doi.org/10.3390/mps7030041 Pr Didier Raoult : Didier Raoult, Confirmation of the presence of vaccine DNA in the Pfizer anti-COVID-19 vaccine 2024. ffhal-04778576f https://hal.science/hal-04778576v1/document Kämmerer U, Schulz V, Steger K. BioNTech RNA-Based COVID-19 Injections Contain Large Amounts Of Residual DNA Including An SV40 Promoter/Enhancer Sequence. Science, Public Health Policy and the Law. 2024 Dec 03; v5.2019-2024 https://publichealthpolicyjournal.com/biontech-rna-based-covid-19-injections-contain-large-amounts-of-residual-dna-including-an-sv40-promoter-enhancer-sequence/ Wang, Tyler J, Alex Kim, and Kevin Kim. 2024. “A Rapid Detection Method of Replication-Competent Plasmid DNA from COVID-19 mRNA Vaccines for Quality Control.” Journal of High School Science 8 (4): 427–39. https://jhss.scholasticahq.com/article/127890-a-rapid-detection-method-of-replication-competent-plasmid-dna-from-covid-19-mrna-vaccines-for-quality-control Sonia Pekova : Quantitative Multiplex Real-Time PCR analysis of Moderna (Spikevax) and Pfizer (BNT162b2) vaccines, Sona Pekova, MD, PhD., TILIA LABORATORIES s.r.o., Laboratory for molecular diagnostics, Pchery, Czech Republic 08.03.2025 https://www.10letters.org/CzechResearch.pdf

Sequencing of bivalent Moderna and Pfizer mRNA vaccines reveals nanogram to microgram quantities of expression vector dsDNA per dose Several methods were deployed to assess the nucleic acid composition of four vials of the Moderna and Pfizer bivalent mRNA vaccines. Two vials from each vendor were evaluated with Illumina sequencing, qPCR, RT-qPCR, Qubit™ 3 fluorometry and Agilent Tape Station™ electrophoresis. Multiple assays support DNA contamination that exceeds the European Medicines Agency (EMA) 330ng/mg requirement and the FDAs 10ng/dose requirements. These data may impact the surveillance of vaccine mRNA in breast milk or plasma as RT-qPCR assays targeting the vaccine mRNA cannot discern DNA from RNA without RNase or DNase nuclease treatments. Likewise, studies evaluating the reverse transcriptase activity of LINE-1 and vaccine mRNA will need to account for the high levels of DNA contamination in the vaccines. The exact ratio of linear fragmented DNA versus intact circular plasmid DNA is still being investigated. Quantitative PCR assays used to track the DNA contamination are described. osf.io

DNA fragments detected in monovalent and bivalent Pfizer/BioNTech and Moderna modRNA COVID-19 vaccines from Ontario, Canada: Exploratory dose response relationship with serious adverse events. Background: In vitro transcription (IVT) reactions used to generate nucleoside modified RNA (modRNA) for SARS-CoV-2 vaccines currently rely on an RNA polymerase transcribing from a DNA template. Production of modRNA used in the original Pfizer randomized clinical trial (RCT) utilized a PCR-generated DNA template (Process 1). To generate billions of vaccine doses, this DNA was cloned into a bacterial plasmid vector for amplification in Escherichia coli before linearization (Process 2), expanding the size and complexity of potential residual DNA and introducing sequences not present in the Process 1 template. It appears that Moderna used a similar plasmid-based process for both clinical trial and post-trial use vaccines. Recently, DNA sequencing studies have revealed this plasmid DNA at significant levels in both Pfizer-BioNTech and Moderna modRNA vaccines. These studies surveyed a limited number of lots and questions remain regarding the variance in residual DNA observed internationally. Methods: Using previously published primer and probe sequences, quantitative polymerase chain reaction (qPCR) and Qubit® fluorometry was performed on an additional 27 mRNA vials obtained in Canada and drawn from 12 unique lots (5 lots of Moderna child/adult monovalent, 1 lot of Moderna adult bivalent BA.4/5, 1 lot of Moderna child/adult bivalent BA.1, 1 lot of Moderna XBB.1.5 monovalent, 3 lots of Pfizer adult monovalent, and 1 lot of Pfizer adult bivalent BA.4/5). The Vaccine Adverse Events Reporting System (VAERS) database was queried for the number and categorization of adverse events (AEs) reported for each of the lots tested. The content of one previously studied vial of Pfizer COVID-19 vaccine was examined by Oxford Nanopore sequencing to determine the size distribution of DNA fragments. This sample was also used to determine if the residual DNA is packaged in the lipid nanoparticles (LNPs) and thus resistant to DNaseI or if the DNA resides outside of the LNP and is DNaseI labile.  Results: Quantification cycle (Cq) values (1:10 dilution) for the plasmid origin of replication (ori) and spike sequences ranged from 18.44 - 24.87 and 18.03 - 23.83 and for Pfizer, and 22.52 – 24.53 and 25.24 – 30.10 for Moderna, respectively. These values correspond to 0.28 – 4.27 ng/dose and 0.22 - 2.43 ng/dose (Pfizer), and 0.01 -0.34 ng/dose and 0.25 – 0.78 ng/dose (Moderna), for ori and spike respectively measured by qPCR, and 1,896 – 3,720 ng/dose and 3,270 – 5,100 ng/dose measured by Qubit® fluorometry for Pfizer and Moderna, respectfully. The SV40 promoter-enhancer-ori was only detected in Pfizer vials with Cq scores ranging from 16.64 – 22.59. In an exploratory analysis, we found preliminary evidence of a dose response relationship of the amount of DNA per dose and the frequency of serious adverse events (SAEs). This relationship was different for the Pfizer and Moderna products. Size distribution analysis found mean and maximum DNA fragment lengths of 214 base pairs (bp) and 3.5 kb, respectively. The plasmid DNA is likely inside the LNPs and is protected from nucleases. Conclusion: These data demonstrate the presence of billions to hundreds of billions of DNA molecules per dose in these vaccines. Using fluorometry, all vaccines exceed the guidelines for residual DNA set by FDA and WHO of 10 ng/dose by 188 – 509-fold. However, qPCR residual DNA content in all vaccines were below these guidelines emphasizing the importance of methodological clarity and consistency when interpreting quantitative guidelines. The preliminary evidence of a dose-response effect of residual DNA measured with qPCR and SAEs warrant confirmation and further investigation. Our findings extend existing concerns about vaccine safety and call into question the relevance of guidelines conceived before the introduction of efficient transfection using LNPs. With several obvious limitations, we urge that our work is replicated under forensic conditions and that guidelines be revised to account for highly efficient DNA transfection and cumulative dosing. osf.io

Page not found - Russell Broadbent MP russellbroadbent.com.au

South Carolina Legislature Online - Error scstatehouse.gov

South Carolina Legislature Online - Error scstatehouse.gov

BioNTech RNA-Based COVID-19 Injections Contain Large Amounts Of Residual DNA Including An SV40 Promoter/Enhancer Sequence - Science, Public Health Policy and the Law Background: BNT162b2 RNA-based COVID-19 injections are specified to transfect human cells to efficiently produce spike proteins for an immune response. publichealthpolicyjournal.com

A rapid detection method of replication-competent plasmid DNA from COVID-19 mRNA vaccines for quality control | Published in Journal of High School Science By Tyler J Wang, Alex Kim & 1 more. DNA contamination is the primary reason that undermines public trust in the quality of mRNA vaccines. We report a method to detect residual replication-competent plasmid DNA present in mRNA vaccines. jhss.scholasticahq.com