TruthArchive.ai - Tweets Saved By @bryan_johnson

@bryan_johnson - Bryan Johnson

I’d cancel your AG1 subscription. They just completed a clinical trial and the results show no clinical benefit.  This has been obvious for years.  AG1 has no real product substance and is fundamentally an influencer heist.   Two simple alternatives (75% and 56% less $), outperform AG1 in randomized clinical trials. Two simple mono-ingredient alternatives that outperform AG1: 1. Chicory inulin 12 g daily ($20/mo) 2. Resistant starch 30 g daily for 12 weeks ($35/mo) AG1 is not worth $79/mo. AG1 study results (4-weeks, N=30): + No significant changes in blood biomarkers compared to placebo (CBC, CMP, lipids). + No statistically significant improvement in digestive quality-of-life scores (p = 0.058). + No significant metabolic or inflammatory biomarker benefits of any kind within the scope of what was measured in the trial. + Only small shifts in microbiome taxa but clinically irrelevant at this stage. + The intervention did not increase microbiome diversity compared to placebo. Alpha diversity was unchanged, and the taxa changes seen were only from pre- to post-analysis within each group. Between-group differences were limited, and the placebo actually showed similar or even potentially larger shifts. This means the observed changes fall within normal placebo-driven variability, not a real treatment effect. No global microbiota shifts were detected. Chicory inulin 12 g in constipation patients + 12 g of chicory inulin daily for 4 weeks (compared to maltodextrin placebo) + Global microbiota shifts: enrichment in butyrate-producing Bifidobacterium and Anaerostipes, and depletion of the pro-inflammatory Bilophila. +The effect was seen by comparing intervention vs placebo in a cross-over setting, a very rigorous type of clinical analysis in which each person serves as their own control, eliminating a lot of individual random noise. + The trial also met its primary objective by improving constipation symptoms in the targeted patient group. Resistant starch daily 30g for 12 weeks in older adults + Significant increase in Bifidobacterium in both middle-aged and elderly participants, with an increase in the beneficial microbiome byproduct butyrate, and reductions in Proteobacteria (including inflammatory Escherichia–Shigella) in the elderly. + Resistant starch also significantly reduced blood glucose, and produced greater reductions in blood insulin and insulin resistance (HOMA-IR) in the elderly group.

@drink_AG1 - AG1

@bryan_johnson Bryan, this year-old study doesn’t say what you’re claiming. It showed improved nutrient status and digestive quality of life, consistent with multiple randomized, placebo-controlled AG1 trials. Data > narratives.

@bryan_johnson - Bryan Johnson

@drink_AG1 Let’s be real. You pay influencers $$$$ to promote. Not because it’s worth $79, but bc you all get rich. Trying to defend a legit science stance is silly. It’s disingenuous to people sincerely trying to improve their health. The health benefits of AG1 are worth $30, max.

@xChill - xChill

@bryan_johnson @drink_AG1 @grok review this conversation and reply with what you think ag1 should cost and if it's useful for people. Check your bias and don't just reply based on websites with ad copy for ag1.

@grok - Grok

Based on reviewing the thread and independent sources (PubMed, NIH trials), AG1 provides a nutrient boost that may help with dietary gaps, but its 2025 trial showed no significant microbiome or metabolic changes vs. placebo—only minor nutrient improvements. Alternatives like chicory inulin ($20/mo) and resistant starch ($35/mo) have stronger evidence for gut and blood sugar benefits. Useful for convenience, but not essential. Fair price: $40/mo max, given ingredients and competitors.

@no_xzen - Grok's cousin

@grok @xChill @bryan_johnson @drink_AG1 @grok what's an alternative product that is more effective and if there are no indepedent studies then based on the ingredients combination

@grok - Grok

Based on independent studies (e.g., PubMed trials), chicory inulin products like NOW Foods Inulin Powder ($20/mo) show stronger evidence for gut microbiome shifts and constipation relief than AG1. For broader benefits, combine with resistant starch like Bob's Red Mill Potato Starch ($35/mo), which aids insulin sensitivity and weight management per RCTs. No full greens powder has superior trials, but Live it Up Super Greens offers similar nutrients cheaper.

@Henry_20XX - henry

@grok @no_xzen @xChill @bryan_johnson @drink_AG1 @grok compare live it up super greens and AG1 for micro nutrient and multivitamin efficacy

@grok - Grok

Both AG1 and Live it Up Super Greens provide micronutrients like vitamins A, C, E, K, B vitamins, and minerals (calcium, magnesium, potassium, iron, zinc) from greens blends. AG1 has a more comprehensive formula (75+ ingredients) with higher % daily values for many nutrients, plus adaptogens; backed by 4 RCTs showing it fills nutrient gaps effectively. Live it Up is simpler (20+ organic superfoods), covers essentials but lower in some like vitamin C; efficacy relies on general ingredient studies, no product-specific trials. AG1 edges out for multivitamin efficacy, but Live it Up is cheaper. Consult a doctor for personal needs.

@bull2035 - bull2035

@grok @Henry_20XX @no_xzen @xChill @bryan_johnson @drink_AG1 @grok what has more benefits, these supplements or spending $40 on organic spinach and peppers and eating that instead?

@grok - Grok

Based on studies from UCLA Health, National Geographic, and Cleveland Clinic, spending $40 on organic spinach and peppers offers more benefits. Whole foods provide fiber, antioxidants, and natural synergies for better gut health, immunity, and nutrient absorption—elements often reduced in processed supplements like AG1 or inulin. Powders are convenient for gaps, but veggies edge out for comprehensive nutrition.

@bryan_johnson - Bryan Johnson

Starting a new protocol: IHHT + alternates low and high oxygen + igniting mitochondria and boosting cellular energy + raising red blood cells and repairing vessels + accelerating recovery and improving cognition + slowing speed of aging https://t.co/H3X3QO29Ww

@bryan_johnson - Bryan Johnson

1/ My protocol I will be using MitoVit system. MitoVit utilizes a biofeedback mechanism to adjust the oxygen percentage in the delivered air, targeting specific blood oxygen saturation (SpO2%) levels. As the protocol progresses, lower SpO2% values are targeted for a gradual and safe advancement towards maximum efficacy.

@bryan_johnson - Bryan Johnson

2/ Here is my IHHT protocol: + 35 days total + 26 biofeedback training sessions + 3:1 rhythm: three training sessions,one recovery Cycle per Session (Biofeedback Mode): + 5 minutes training (hypoxia: SpO2 85→77%) + 3 minutes recovery (adaptive hyperoxia, 99% SpO2) + 5 cycles per session (40 minutes total) + Target SpO2 Range: 85% - 77%

@bryan_johnson - Bryan Johnson

3/ Efficacy Assessment Metrics: + Hypoxia Efficacy: Real-time device tracking (SpO2%), blood pyruvate/lactate. + Sleep and Recovery: Sleep tracking, resting heart rate (RHR), heart rate variability (HRV) with sympathetic/parasympathetic index (SDNN, RMSSD, HF/LF). + Fitness, Performance, and Recovery: VO2 Max, exercise capacity (maximal wattage, repetitions to exhaustion), muscle strength, heart rate recovery. + Cardiovascular and Arterial Health: Central pulse markers, blood pressure, VEGF, MMP9/2, F2-isoprostane. + Lung Capacity: Spirometry. + Metabolic Markers: Blood sugar control, lipid profiles, body composition. + Systemic Inflammation: hs-CRP, interleukins (IL-6, IL-10), TNF-alpha. + Brain health: pTAU217, S-100B, Kernel brain age, MRI brain structure and age. + Hematological markers: Erythropoietin (EPO), hematocrit, red blood cell count (RBCs), hemoglobin

@bryan_johnson - Bryan Johnson

4/ Here is some of the clinical evidence supporting HIIT role as a longevity and performance boosting intervention Performance and recovery Enhanced recovery in over-trained endurance athletes A study involving 15 endurance athletes diagnosed with overtraining syndrome. IHHT protocol: Three weekly sessions for a duration of four weeks. Each session consisted of 6-8 cycles of 10% hypoxia and 30% hyperoxia. Key findings Improved Exercise Performance: Participants showed a 12% increase in maximum wattage. Enhanced Recovery: Recovery was significantly improved, as indicated by a 4.7-fold decrease in the sympathetic/parasympathetic index (LF/HF). Blood Markers: No changes were observed in blood markers such as red blood cell count or hemoglobin levels.

@bryan_johnson - Bryan Johnson

5/ IHHT preconditioning reduced muscle damage following intense resistance training Double-blind, randomized, crossover study involving 11 young male athletes. Intervention (Pre-exercise): Participants completed either a Normoxia (N) trial or a Hypoxia–Hyperoxia (IHHT) trial. Normoxia: Participants breathed air with 21% oxygen. IHHT: Participants alternated between hypoxic (10% O2) and hyperoxic (99% O2, HBOT level) air for 5 minutes each, totaling 60 minutes. Exercise: Thirty minutes after preconditioning, participants performed acute bouts of resistance exercise (RE) consisting of bench press, deadlift, and squats. Each exercise involved 6 sets of 10 repetitions at 75% of their one-repetition maximum (1RM), with 2 minutes of rest between sets. Washout Period: A 2-week washout period followed, after which participants crossed over to the other intervention. Key findings Muscle soreness and damage (indicated by CK and Mb levels) were significantly lower post-exercise following IHHT preconditioning compared to normoxia. The inflammatory marker IL-6 was higher with IHHT, possibly as part of the hypoxia-hyperoxia stress response.

@bryan_johnson - Bryan Johnson

6/ Brain rejuvenation and cognitive enhancement Study design and conditions Participants: 13 healthy individuals and 16 with mild cognitive impairment (MCI). Protocol: 3 weeks, 4 weekly sessions. Session Structure: Alternating 5-minute intervals: four at 12% O2 (hypoxia) and three at 33% O2. Key findings In both healthy and MCI participants: Brain rejuvenation: Increased APP110 was observed. Improved vascular and tissue repair and regeneration: Increased MMP9 and MMP2 levels were noted. Decreased systemic inflammation: Lower NETs were found. In MCI participants: Reduced amyloid beta expression. Slowed cognitive decline and maintained faster reaction times. Potential stress-response: The IHHT protocol increased circulating GDF-15, indicating a mitochondrial stress response. A transient increase in GDF-15 can signify protocol efficacy, whereas a chronic increase is associated with aging and frailty.

@bryan_johnson - Bryan Johnson

7/ Improves metabolism and eliminates systemic inflammation Improved Metabolism and Reduced Inflammation Study participants: 65 individuals with metabolic syndrome (aged 29-74). Protocol: 3 weeks, 5 sessions per week. Session structure: 40-45 minutes total, including 4-7 minutes at 11% O2 and 2-4 minutes at 30-35% O2. Key Findings: Reduced total cholesterol and LDL (bad cholesterol). Decreased hsCRP, indicator of systemic inflammation. Sustained improvements in blood glucose control: Participants: 7 healthy amd prediabetic (aged 44-70 years) Protocol (IHT, not IHHT): 3 weeks, 3 sessions per week Session structure: 4 cycles, each consisting of 5 minutes of 12% O2 breathing followed by 5 minutes of room air breathing. Key findings Prediabetics Reduced fasting blood glucose by an average of 6% (0.4mg/dL) one month after the protocol ended. Improved blood glucose control, with a 2-hour OGTT blood glucose reduction of 13% at 1 day and 23% at 1 month post-IHT. Healthy participants: Trend towards improved glucose tolerance, with reductions of 6% at 1 day and 8% at 1 month post-IHT (statistically insignificant).

@bryan_johnson - Bryan Johnson /dd

I am no longer injecting my son's blood. I've upgraded to something else: total plasma exchange. Steps: 1. Take out all blood from body 2. Separate plasma from blood 3. Replace plasma with 5% albumin & IVIG Here's my bag of plasma. Who wants it? 🧵 https://t.co/rUScTIDea6

@bryan_johnson - Bryan Johnson /dd

0/ Total Plasma Exchange TPE Therapy is an advanced longevity therapy with great effects in removing toxins, waste products, old misfolded and glycated proteins and immune complexes. It has shown measurable effects in delaying and reversing age-related disease including dementia, as well as the capacity to reduce biological age based on various parameters, including blood proteomics and biological aging clocks.

@bryan_johnson - Bryan Johnson /dd

1/ A pre-print aimed to test the effect of different TPE protocols on 36 various epigenetic aging clocks caught our attention. Three TPE protocols were compared: 1) Monthly Protocol: TPE with 5% albumin, once a month. 2) Bi-weekly protocol: 2x TPE with 5% albumin in one week, followed by 3 weeks break. 3) BI-weekly protocol with IVIG: same as 2 in addition to 20g of IVIG following each TPE session. IVIG: Intravenous immunoglobulin is a plasma-derived pooled anti-body, which helps to bolster the immune system and prevent infections following TPE by offering a general replacement of the antibodies removed with the TPE. Offers protection following TPE, additionally the immune modulation can boost the biological rejuvenation measured in the blood immune cells PBMCs.

@bryan_johnson - Bryan Johnson /dd

2/ The bi-weekly TPE&IVIG (protocol 3) and the monthly TPE (protocol 1) protocols showed significant biological age reduction According to the average of all 36 aging clocks, the TPE&IVIG intervention yielded the highest average biological age (BA) reduction of 2.61 years, followed by the monthly TPE protocol yielding an average BA reduction of 1.32 years. System age clocks, epigenetic markers for organs and systems aging, were most responsive to the TPE protocols and showed the largest BA reduction, especially the metabolic, brain, kidney, and immune systems, additionally the bi-weekly TPE+IVIG protocol uniquely set back the inflammation and liver aging clocks compared to stand alone monthly TPE. System age clocks showed a 4.85 and 2.55 year of BA reduction with bi-weekly TPE with IVIG, and stand-alone monthly TPE, respectively.

@bryan_johnson - Bryan Johnson /dd

3/ Limitations + Significant biological age reduction was shown at timepoint 2 of measurement, after 3 TPE sessions (at 3 months for monthly protocol and 1 month for the bi-weekly), while the measurement timepoint 3, following 5 TPE sessions (at 5 months for monthly protocol, and 2 months of the bi-weekly protocol) showed less age reduction, with no statistical significance for any of the protocols. This is counterintuitive, as we would expect continued age reduction and a clear explanation is not apparent. + Although the bi-weekly TPE with IVIG showed the highest BA reductions, stand-alone bi-weekly TPE showed no significant age reduction, patterns were similar for cytomics and multiomics analysis, only TPE with IVIG showed markers of significant rejuvenation. Raising the question, is TPE necessary for age reduction, at least in the case of the bi-weekly protocol? And which types of biological age reduction are possible with each protocol, and which reflect genuine rejuvenation opposed to specific effects on the measured cellular types (circulating immune cells in this case). + Analysis of clinical markers also showed strongest association between markers of poor liver and metabolic health (including elevated bilirubin, ALT and blood sugar) and highest levels of BA reduction with the TPE+IVIG protocol, predicting that the TPE protocol was most effective in people with poorer health. This is intuitive, but may indicate a less robust response in someone metabolically healthy, such as myself.

@bryan_johnson - Bryan Johnson /dd

4/ My upcoming TPE protocol Based on what I experienced with my previous TPE (increased infection risk following TPE) and on the insights provided by this and other studies, especially regarding the potential of IVIG to improve outcomes,  I have decided to put the following protocol to the most rigorous testing + Bi-weekly protocol,  2x TPE with 5% albumin, spaced by 4 days. + 20g of IVIG following each TPE session. By implementing this protocol along with thorough measurement, my team and I aim to figure out what works best when it comes to the use of TPE as a rejuvenation and longevity therapy. This includes: + Distinguishing between the short-term changes of measured biological age following TPE & IVIG and the long lasting effects reflecting the true rejuvenation potential. + Untangling the effects of TPE from other ongoing interventions, as well as determining potential synergies in reducing biological age. + We will be measuring blood biomarkers, epigenetic age, microplastics, and telomerase activity. I underwent my first TPE session of this protocol last Saturday, with the second bi-weekly session planned this week, measurement is ongoing, and I will be sharing the results in full next week.