TruthArchive.ai - Tweets Saved By @franciscodeasis

@franciscodeasis - Francisco de Asis

@Rossana38510044 @luigi_warren @babarlelephant @AntGDuarte @MonaRahalkar @BillyBostickson @flavinkins @KevinMcH3 @DrAntoniSerraT1 @_coltseavers @rowanjacobsen @uacjess @RolandBakerIII @TheSeeker268 @Daoyu15 @still_a_nerd @jjcouey @Harvard2H @ydeigin @CarltheChippy @ico_dna @Nomdeplumi1 @Real_Adam_B @nerdhaspower @scottburke777 @JJ2000426 @BahulikarRahul @alimhaider @antonioregalado @Ayjchan @R_H_Ebright @BretWeinstein @sanchak74 @JCalvertST [Thread] Addendum of WIV in Nature. What we know about the samples and the visits to the mineshaft of TG? TLDR: 7 trips: 4 already known (Ge et al., 2016), plus other 3 with massive sampling until 2015, including 7896-clade. All already in Latinne et al. (2020) #originsofSARSCoV2

@franciscodeasis - Francisco de Asis

@AntGDuarte @Rossana38510044 @jhouse678 @nature @edwardcholmes @arambaut @angie_rasmussen @K_G_Andersen What we know about the 7896 clade? Let's recap: 1/ They were published in June 2020 as part of a batch of 630 viruses of Latinne et. (2020). No details were given https://www.ncbi.nlm.nih.gov/nuccore/?term=MN312240%3AMN312869%5Baccn%5D

mn312240:mn312869[accn] - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov

@franciscodeasis - Francisco de Asis

[Thread] New data on the Tongguan mine (Mojiang, Pu'er, Yunnan) where RaTG13 virus was collected in 2013. Previous threads: - Probable Location: - Probable outline: - Asahi expedition:

@franciscodeasis - Francisco de Asis

@AP @Drinkwater5Reed Here the classification of the race of journalists to the Mojiang mine updated. It is becoming increasingly difficult. Not only car blocks, but also barricades. I can suggest alternative strategies (DM) #originsofSARSCoV2 #DRASTIC

@franciscodeasis - Francisco de Asis

[Compilation Thread] English translation of the MSc thesis of Xu Li (2013) and the PhD thesis of Canping Huang (2016). Files (non-pdf text format), with some additional info, upon request. Credits: both theses discovered by @TheSeeker268 #DRASTIC #originsofSARS2

@franciscodeasis - Francisco de Asis

[Thread] “SL3” the real name behind RaTG13 #originsofSARSCoV2 #DRASTIC

@franciscodeasis - Francisco de Asis

[Thread] RaTG13 amplicons names #originsofSARSCoV2 #DRASTIC TLDR: WIV inadvertently disclosed important info in fasta headers. Dates as 2017-2018 and a label pointing to Ra7896, a close virus to RaTG13 kept secret at the start of the outbreak and even today not fully disclosed

@franciscodeasis - Francisco de Asis

@Rossana38510044 @luigi_warren @babarlelephant @AntGDuarte @MonaRahalkar @BillyBostickson @flavinkins @KevinMcH3 @DrAntoniSerraT1 @_coltseavers @rowanjacobsen @uacjess @RolandBakerIII @TheSeeker268 @Daoyu15 @still_a_nerd @jjcouey @Harvard2H @ydeigin @CarltheChippy @ico_dna @Nomdeplumi1 @Real_Adam_B @nerdhaspower @scottburke777 @JJ2000426 @BahulikarRahul @alimhaider @antonioregalado @Ayjchan @R_H_Ebright @BretWeinstein @sanchak74 @JCalvertST @PeterDaszak @TheSeeker 7/ Virus isolates at WIV First of all, "isolate" is an ambiguous word (https://t.co/bqEpZjhepA). Here I mean actual isolation. WIV used correlative numbering for them. WIV6 and WIV15 were never published. But WIV15 may perfectly come from 5-7th visit TG

@franciscodeasis - Francisco de Asis

[Thread] Complaints to top journals #originsofSARSCoV2 #DRASTIC Let’s start with a victory: the Addendum in Nature in November (not thanks to me that I am probably ignored, but thanks to some diligent scientists who pushed it, some of them from #DRASTIC)

@franciscodeasis - Francisco de Asis

[Thread] Pangolin CoV... or Bat CoV in pangolins samples? TLDR: A researcher of the team that sequenced the pangolins samples had taken samples in the mine of RaTG13 and in the place where RmYN02 was collected, also catched bats in Yunnan. Probably contaminated pangolin samples

@franciscodeasis - Francisco de Asis

[Thread] Unnoticed Lab contamination in the samples of first COVID-19 infected patients. 1/ Article: “There was a simultaneous undetected outbreak of Nipah in Wuhan” Explanation:

@franciscodeasis - Francisco de Asis

SARS-CoV-2 could come not from 1 virus of the Mojiang mine, but from 2 viruses of the mine! That is why we see strange things in the trees. RaTG13 & Ra7896 faked although not very far from reality. 1/ 2/ 3/

@franciscodeasis - Francisco de Asis

[Thread] In-silico molecular overclocking of RaTG13. TLDR: 191-nt RdRp segments of SARS-CoV-2, RaTG13 and Ra7896 show an unexpected molecular clock behavior. In-silico synonym mutations is one probable explanation.

@franciscodeasis - Francisco de Asis

[Thread] Missing / unpublished sequences WANTED: Live isolates WIV15 & WIV6; Ra7896 (complete genome); EPI_ISL_402122; MT394201

@franciscodeasis - Francisco de Asis

[Thread] New WIV theses found by @TheSeeker268. Let's make some comments on very important findings

@franciscodeasis - Francisco de Asis

New pre-print from WIV: RaTG15, which it seems it is former Ra7909 https://www.biorxiv.org/content/10.1101/2021.05.21.445091v1

Identification of a novel lineage bat SARS-related coronaviruses that use bat ACE2 receptor bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution biorxiv.org

@franciscodeasis - Francisco de Asis

[Thread] Bat tissue collection and cell lines from the 3rd trip to Tongguan (TG) mine in Mojiang in Apr-2013

@franciscodeasis - Francisco de Asis

[Thread] Regardless of the origin of the pandemic, it seems that the first superspreading event -in the Huanan seafood market- might not have been the result of chance.

@franciscodeasis - Francisco de Asis

[Thread] Necessary corrections to the China-WHO report. What they will probably fix and what they will not. TLDR: circular swap of 3 IPCAMS genomes + tampered onset of Wuchang accountant; First patients and first cluster; Some falsehoods in articles https://www.washingtonpost.com/world/asia_pacific/covid-wuhan-outbreak-who/2021/07/15/51e7e8a6-e2c6-11eb-88c5-4fd6382c47cb_story.html

@franciscodeasis - Francisco de Asis

[Thread] Who is the first known patient? There is a lot of confusion, so let's review all possible patients according published onset dates [of symptoms] up to 15-Dec-19.

@franciscodeasis - Francisco de Asis

Chen XX, Wuchang accountant and nephew of a doctor, 41 yrs old male (41M1), is not 1st known onset date on 08-Dec-19 as China-WHO report states (annex p. 117). His onset date was 16-Dec-19 according to 2 Wuhan Central Hospital doctors (Prof. Zhao Su & less-known hero Dr. Ai Fen)

@franciscodeasis - Francisco de Asis

[Thread] Anthony et al. (2019) published a big database (discovered by @interne41914499) with viruses until Jun-2014. Kind of an Addendum of Anthony et al. (2017). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5467638/ There are interesting findings https://datadryad.org/stash/dataset/doi:10.5061/dryad.34db1gr

Global patterns in coronavirus diversity Since the emergence of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) and Middle East Respiratory Syndrom Coronavirus (MERS-CoV) it has become increasingly clear that bats are important reservoirs of CoVs. Despite this, only 6% of all CoV ... pmc.ncbi.nlm.nih.gov

Dryad | Data -- Global patterns in coronavirus diversity datadryad.org

@franciscodeasis - Francisco de Asis

[Thread] The mystery of “live isolate” Rs4874 with no WIV number assigned. TLDR: Probably not a [pure] live isolate but a mere infectious clone. Just another case of academic cheating, to make sure the article was accepted by PLoS Pathog (Hu et al., 2017)

@franciscodeasis - Francisco de Asis

[Thread] FOIA from @USRightToKnow regarding Latinne et al. (2020) and clade 7896 TLDR: No sequence was deleted/modified since Aug-2019, but it seems they wanted to buy time for not publishing the viruses very early in the pandemic. https://usrtk.org/biohazards/foi-documents-on-origins-of-sars-cov-2-risks-of-gain-of-function-research-and-biosafety-labs/

FOI documents on origins of Covid-19, gain-of-function research and biolabs Freedom of information documents from our investigation into the origins of Covid-19, gain-of-function and biolab risks. usrtk.org

@franciscodeasis - Francisco de Asis

[Thread] New unforced error. GIABR, the lab of the pangolins, has just uploaded sequences (MW600658:MW600715) that shows a trip to the Mojiang mineshaft or nearby on 22-Aug-2017, well after the last known trip of WIV in 2015. Libiao Zhang explicitly credited as collector.

@franciscodeasis - Francisco de Asis

[Thread] Nth falsehood from WIV/EHA. The Addendum acknowledged only sampling bats "once or twice a year" in Mojiang, but this EHA report shows at least 4 trips in 2014. Latinne et al. (2020) did not publish anything from "Jul.--Sep. 2014" & Mojiang.

@franciscodeasis - Francisco de Asis

[Thread] Wang et al. (2021): Another article revealing trips to the Mojiang mine and Laos was kept in the limbo when the pandemic started. Note: The article covers HKU10 viruses which are not related with SARS-like viruses. https://www.mdpi.com/1999-4915/13/10/1962

Genomic Characterization of Diverse Bat Coronavirus HKU10 in Hipposideros Bats Bats have been identified as natural reservoirs of a variety of coronaviruses. They harbor at least 19 of the 33 defined species of alpha- and betacoronaviruses. Previously, the bat coronavirus HKU10 was found in two bat species of different suborders, Rousettus leschenaultia and Hipposideros pomona, in south China. However, its geographic distribution and evolution history are not fully investigated. Here, we screened this viral species by a nested reverse transcriptase PCR in our archived samples collected over 10 years from 25 provinces of China and one province of Laos. From 8004 bat fecal samples, 26 were found to be positive for bat coronavirus HKU10 (BtCoV HKU10). New habitats of BtCoV HKU10 were found in the Yunnan, Guangxi, and Hainan Provinces of China, and Louang Namtha Province in Laos. In addition to H. pomona, BtCoV HKU10 variants were found circulating in Aselliscus stoliczkanus and Hipposideros larvatus. We sequenced full-length genomes of 17 newly discovered BtCoV HKU10 strains and compared them with previously published sequences. Our results revealed a much higher genetic diversity of BtCoV HKU10, particularly in spike genes and accessory genes. Besides the two previously reported lineages, we found six novel lineages in their new habitats, three of which were located in Yunnan province. The genotypes of these viruses are closely related to sampling locations based on polyproteins, and correlated to bat species based on spike genes. Combining phylogenetic analysis, selective pressure, and molecular-clock calculation, we demonstrated that Yunnan bats harbor a gene pool of BtCoV HKU10, with H. pomona as a natural reservoir. The cell tropism test using spike-pseudotyped lentivirus system showed that BtCoV HKU10 could enter cells from human and bat, suggesting a potential interspecies spillover. Continuous studies on these bat coronaviruses will expand our understanding of the evolution and genetic diversity of coronaviruses, and provide a prewarning of potential zoonotic diseases from bats. mdpi.com

@franciscodeasis - Francisco de Asis

Little is being said about this study of Qi Jin (IPBCAMS) that reveals how all the SARS lineages of the Mojiang mine have become extinct. And they have been looking for viruses at 9 sites in Mojiang County or very close. https://www.researchsquare.com/article/rs-885194/v1

A comprehensive survey of bat sarbecoviruses across China for the origin tracing of SARS-CoV and SARS-CoV-2 Severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2 have been thought to originate from bat, but whether the cross-species transmission occurred directly from bat to human or through an intermediate host remains elusive. In this study, we performed CoV screening of 102 samples... researchsquare.com

@franciscodeasis - Francisco de Asis

In the most important part of their heatmap (SW corner) they only put 3 stalls (all misplaced) and they omit the toilets and the Mahjong room. Their response to our raising issues?: silence and blocks. It seems we are making essential contributions. Again.

@franciscodeasis - Francisco de Asis

Let's go on an Easter egg hunt inside the pdf 🧵

@franciscodeasis - Francisco de Asis

🧵Natural and non-natural live isolates (WIV & related) TLDR: - A substantial portion of the research remains unpublished. - Missing live isolate WIV15. - No samples, isolates, chimeric and MA viruses in 2017-2019? - What is "Rs4874" really? https://t.co/25XbQLCQQb https://t.co/9JPusWbl9l