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Saved - December 15, 2024 at 7:48 PM
reSee.it AI Summary
I’ve been discussing Oliver Pybus and his connections to various figures in the context of COVID-19 origins. I question his awareness of early cases in Wuhan and the leaked data from November 2019. It seems he avoids acknowledging evidence that contradicts his narrative, instead promoting what I see as misleading information. I highlight his collaborations with individuals linked to Chinese research and suggest that his work may align with Chinese interests, particularly regarding COVID-19 control measures. The associations he maintains raise concerns about credibility.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

1. Exposing the Phylogeographic Clown of the Natural Origin cabal, Oliver Pybus @EvolveDotZoo who spews out Chinese Lies on demand with his colleagues, Peter Daszak, George Gao, Eddie Holmes and their ilk https://x.com/evolvedotzoo/status/740853994038427648

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

2. Does Pybus really know nothing of the November 17 "patient zero" nor the early Wuhan hospital leaked data revealing earlier cases in October and November 2019? 1. 2. 3. https://gillesdemaneuf.medium.com/early-cases-of-suspected-covid-19-in-wuhan-feb-20-data-collection-b7740ed1436f

Early cases of suspected Covid-19 in Wuhan — Feb 20 Data Collection Research by DRASTIC, a group of researchers working together to investigate the origins of SARS-CoV-2, starting from facts and not bending to any pressure. DRASTIC members were the first to discover… gillesdemaneuf.medium.com

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

14. Hunt down Patient Zero Time ;) Full Data on early Covid like cases in Wuhan with the leaked reports from the hospitals (September/October/November 2019) https://epochtimes.com/gb/20/10/8/n12462796.htm All names, dates and documents - very useful! via @TheSeeker268 @EpochTimes English & Chinese

【独家】公函泄疫情爆发比中共公布早数月 | COVID-19 | 中共病毒 | 新冠病毒 | 大纪元 中共病毒(新冠病毒)导致的武汉肺炎大流行,由于中共的故意隐瞒和掩盖,至今真相未能完全揭开。大纪元获得的中共内部文件显示,中共对武汉疫情要求从10月份开始普查,符合新冠病毒影像学特征的发热病人9月底就开始出现,10月中旬已出现不明肺炎死亡病例。 epochtimes.com

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

The Chinese October Surprise (last year's one) 1. SCMP News leak -November 17th Patient Zero https://livescience.com/first-case-coronavirus-found.html 2. Thermogenesis leak via CTO Paul Coelho with links to PKU IMM Lab claiming they knew about it in November

1st known case of coronavirus traced back to November in China A 55-year-old individual from Hubei province in China may have been the first person to have contracted COVID-19, the disease caused by the new coronavirus spreading across the globe. livescience.com

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

3. Pybus cravenly refuses to acknowledge the evidence! Instead he crouches in the darkness, gibbering over his phylogeographic data while swallowing & regurgitating Chinese Lies on behalf of his cabal of propagandists. https://www.sciencemuseumgroup.org.uk/blog/coronavirus-how-the-virus-spread-worldwide/

Coronavirus: How the virus spread worldwide - Science Museum Group Blog The UK has analysed the genetic sequences of more SARS-CoV-2 viruses than any other country. Science Director Roger Highfield investigates how this and other data can help understand how to control the virus and ultimately save lives. blog.sciencemuseumgroup.org.uk

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

4. "A man is known by the company he keeps"...in 2017 Cell Symposium on Emerging and Re-emerging Viruses coronavirus.fr/symposium-and-… and cell-symposia.com/emerging-virus… and cell-symposia.com/emerging-virus…

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

5. "A man is known by the company he keeps"...in 2018 "The Committees - Pasteur Modele- Sous titre test" https://www.modele.conferences-pasteur.org/committees

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

6. Recognise any names? https://nextstrain.org/help/coronavirus/SARS-CoV-2

Documentation page not found - Read the Docs docs.nextstrain.org

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

7. Birds of a Feather Stick Together

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

8. Google propaganda papers - search: oliver pybus peter daszak oliver pybus andrew rambaut oliver pybus edward holmes oliver pybus kristian andersen oliver pybus trevor bedford oliver pybus george gao oliver pybus jonna mazet oliver pybus "zhengli" shi oliver pybus ralph baric

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

9. Pybus has got China's Back! Pybus & Ruifu (That Ruifu) investigation of transmission control measures during the first 50 days of the COVID-19 epidemic in China https://science.sciencemag.org/content/368/6491/638 China's control measures may have prevented 700,000 COVID-19 cases

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Pybus and Ruifu An investigation of transmission control measures during the first 50 days of the COVID-19 epidemic in China https://science.sciencemag.org/content/368/6491/638 and China's control measures may have prevented 700,000 COVID-19 cases https://www.eurekalert.org/pub_releases/2020-03/ps-ccm033120.php

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

10. That Ruifu

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Meet Yang Ruifu, CCP's biological weapons expert https://gnews.org/zh-hans/413491/ via @Gnews202064 Interesting expose of China's top bioweapons expert who oversaw fake pangolin research Paper 1: https://wenku.baidu.com/view/2453b5ce05087632311212e1.html Paper 2: https://wenku.baidu.com/view/a093142258fb770bf78a55e6.html# Pangolin https://news.sina.com.cn/c/2020-02-07/doc-iimxyqvz0871821.shtml

华南农业大学发现穿山甲为新冠病毒潜在中间宿主 原标题:华南农业大学发现穿山甲为新型冠状病毒潜在中间宿主2月7日凌晨1时许,华南农业大学在其官方微信宣布:华南农业大学、岭南现代农业科学与技术广东省实验室沈永义教授、肖立华教授等科研人员联合中国人民解放军军事科学院军事医学研究院杨瑞馥研究员 news.sina.com.cn

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

11. Yes, that Ruifu, the organiser of the Pangoscam Pybus, Holmes, Ruifu?

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Pangoscam

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

12. "Useful Idiots" China's control measures may have prevented 700,000 COVID-19 cases https://www.sciencedaily.com/releases/2020/03/200331130012.htm From ducks to chickens to deadly virus Nathan Wolfe (Metabiota) & Oliver Pybus (2013) https://edition.cnn.com/2013/08/21/opinion/wolfe-virus-ducks-to-chickens/index.html

China's control measures may have prevented 700,000 COVID-19 cases China's control measures during the first 50 days of the COVID-19 epidemic may have delayed the spread of the virus to cities outside of Wuhan by several days and prevented more than 700,000 infections nationwide, according to an international team of researchers. The findings could be useful to countries that are still in early phases of the COVID-19 outbreak. sciencedaily.com
From ducks to chickens to deadly virus | CNN Nathan Wolfe and Oliver Pybus say that the more we learn about the new flu strain, the clearer the need for prevention measures edition.cnn.com

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

13. Pybus is Sure about one thing!

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

14. But then he would, wouldn't he? I mean considering the company he keeps...

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

15. Yang Ruifu, CCP's biological weapons expert, co-author with Oliver Pybus on the above paper must have been exceptionally pleased with his work. https://threadreaderapp.com/thread/1315272494727614465.html

Read and Share Twitter Threads easily! Thread Reader helps you discover and read the best of Twitter Threads threadreaderapp.com

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

16. Unroll Pybus @threadreaderapp

Saved - January 15, 2025 at 11:40 PM
reSee.it AI Summary
I shared a series of posts discussing the lack of transparency surrounding the environmental samples from the Huanan seafood market in Wuhan. Despite numerous inquiries, George Gao has not provided specific details about the positive samples. I highlighted the original questions posed by DRASTIC and the frustration over unanswered queries. A recently leaked map, which took ten months to surface, raises further questions about its delayed release and the discrepancies between versions held by different health organizations. The ongoing investigation by the WHO emphasizes the market's significance in understanding the outbreak.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

1. The Market Map Once upon a time some questions were asked (35-40) https://x.com/BillyBostickson/status/1279110406191976448 Emails were sent asking for details Requests were made BUT George Gao refused to give specific details about the positive environmental samples from the Huanan seafood market in Wuhan.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

5/X 35.What does a “One Health” (human, animal, environmental) blueprint map of the market show in terms of: 33 positive & 552 negative “environmental samples” 27 + persons epidemiologically linked to the Market All the negative & any positive specimens from specific animals.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

2. The Market Map - The 18 Original Questions Here are the original 18 questions prepared by DRASTIC and Dr. Daniel Lucey about the environmental samples that tested positive at the Seafood Market.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

4. The Map (via @TheSeeker268) but now we have, after waiting 10 months, a little map that was hidden from us by the Chinese CDC, and strangely enough by the American CDC and the WHO. Here is the story of a 10 month old map https://archive.is/SmhkZ Read it carefully

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

5. The Leaked Floor Plan Map of the Wuhan Huanan Seafood Market in all its pristine glory

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

6. Question Time Why was the map never made public by China CDC? Who leaked it? Why was it leaked now? Why did SCMP publish it? (remember they also published the November 17th early patient leak) https://en.wikipedia.org/wiki/South_China_Morning_Post What can the map tell us about early cases and the market?

South China Morning Post - Wikipedia en.wikipedia.org

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

7. Take some time to try to answer those questions while reading the article. https://t.co/eAlxaAF6rK

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

8. Another Question Using a FOIA request in the US, the Post also obtained a separate blurred map of the market held by the WHO & the US CDC. "there are areas where markings by investigators differ between the versions" Note small print: Not for Further Distribution Why Not? https://t.co/MNYR4nfBBv

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

9. A polite interpretation? "While the Post’s freedom of information request showed the WHO & the US CDC had a floor plan of the market by late January, it was not clear if the Chinese CDC plan obtained by the Post with different markings was shared with those health bodies"

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

10. Official Responses Basically fuck off and leave us alone while we work on damage control and finding the mole. https://t.co/iFXHCcv8tl

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

11. WHO investigation plan (see images) The WHO produced their report stating what they wanted to do (or be done) in Wuhan here analysed by @gdemaneuf Terms of references for China Part – FINAL DRAFT WHO-convened Global Study of the Origins of SARS-CoV-2: https://drive.google.com/file/d/1rx0W2efbE0R1Aq-lALWTqD22VsWbTlO-/view https://t.co/aTox61GmSn

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

12. Target: The Market The market still figures prominently in the WHO’s investigation as a critical link in the outbreak that could yet yield clues. This was emphasised 3 weeks ago by Embarek of the WHO. https://t.co/cvloDGU4Qp

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

13. Repeat after me, slowly & ironically, while raising your eyebrows repeatedly: China has not disclosed if live animals were among those sampled. China has not disclosed if live animals were among those sampled. China has not disclosed if live animals were among those sampled. https://t.co/f4ZpdViF3o

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

14. Now, Stand up wherever you are, go to the nearest window, and yell as loud as you can: Why NOT? and now you understand, my friend, and yes, illumination is yours! https://t.co/a52kPY8T5F

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

15. most important thing in life is knowing who to blame https://t.co/rQzSgWEHCs

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

1/3 George Gao Fu is a pathological liar who is in charge of the cover up and limited hangouts. Still he has the absolute gall to try to hoodwink the world by refusing to give specific details on the environmental samples. https://t.co/r7rqXByfMX

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

16. Gao's statements ‘the novel coronavirus had existed long before’. "We found the genome of the virus through detection of environmental samples from stalls of illegally operated wild animals" Ian Lipkin said George Gao,was initially convinced that the culprit was a bamboo rat https://t.co/DNmySZ6TDd

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

17. Gao Knew? Gao realised after analysis of the positive environmental samples where SARS-COV-2 had come from. It would have been clear from the products sold on stalls in the western area of the market. That information was never made public. Since then silence & cover up.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

18. Gao confesses "We found the genome of the virus through detection of environmental samples from stalls of illegally operated wild animals" https://t.co/Y3o9PfWOmo

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

29. George Gao We found the genome of the virus through detection of environmental samples from stalls of illegally operated wild animals" Gao Fu, a member of the high-level expert group of the National Health and Health Commission on TV told the public https://archive.is/yTFp3#selection-305.0-313.127

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

19. Lab Animals! This statement viewed in light of the subsequent refusal to share information makes little sense unless you have evidence from the market positive environmental samples that the novel virus was likely from lab experimental animals sold illegally at the market. https://t.co/qot07Fu25z

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

20. Saving China's Face? That explains George Gao's bizarre reticence to give specific details of the environmental positive samples, the slaughter of bamboo rats, the disappearing act involving the live animals from the market and zero published samples of live animals https://t.co/UzsPgUCd3N

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

21. Some Previous material Dale Fisher's "Hot Potato" https://t.co/mbJbCBQs5G Everything starts here, everything disappears from here, & the South China Seafood Market will become clean!" https://t.co/vGazzHgidT Thread on the Seafood Market https://t.co/lXyNgy2fUh

@profdalefisher - Professor Dale Fisher

@BillyBostickson This is the hot potato issue and nobody has a real handle on it. Prof Linfa Wong's understanding is that the only positives were from environmental samples. The positives came from a section selling mammalian products, less so from the other sections like seafood /1

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

1. "Everything starts here, everything disappears from here, & the South China Seafood Market will become clean!" Interesting Chinese report about the South China Seafood Market & WCDC's pathogen Lab at 288 Machang Road which makes many insinuations yet has not been censored yet

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

1/9 Thread on the Wuhan "seafood market" Environmental Samples Issue Conspiracy theories and doubt proliferate when there is lack of transparency. For example, regarding environmental samples which tested positive at the Wuhan seafood market

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

22. The Map reveals more than the lines drawn on it "“A map does not just chart, it unlocks and formulates meaning; it forms bridges between here and there, between disparate ideas that we did not know were previously connected.” ― Reif Larsen, The Selected Works of T.S. Spivet https://t.co/nLWq6lFMqk

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

23. unroll the Map @threadreaderapp https://t.co/bn3VsgR89F

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

24. The two floor-plan maps of the Wuhan Huanan seafood market side by side for comparison of the differences. If you notice something significant please comment below. https://t.co/7G7oP5i6NL

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

unroll @threadreaderapp save unroll @rattibha @reSeeIt save thread save @PingThread save @buzz_chronicles

Saved - December 21, 2024 at 9:55 AM
reSee.it AI Summary
I've compiled a series of posts discussing the origins of SARS-CoV-2, focusing on the Mojiang mineshaft and related findings. Key points include the confirmation of the 7896 clade, the significance of WIV's sampling trips, and the challenges faced by journalists trying to access the mine. I highlighted the importance of unpublished sequences and the potential implications of lab contamination. Additionally, I noted discrepancies in reported patient onset dates and the need for further investigation into the origins of the virus, including the role of various isolates and samples.

@franciscodeasis - Francisco de Asis

[Thread of threads] #originsofSARSCoV2 #DRASTIC Compilation of relevant findings. Live thread. Let's start with the Addendum and the 7896 clade recently confirmed to come from the Mojiang mineshaft

@franciscodeasis - Francisco de Asis

@Rossana38510044 @luigi_warren @babarlelephant @AntGDuarte @MonaRahalkar @BillyBostickson @flavinkins @KevinMcH3 @DrAntoniSerraT1 @_coltseavers @rowanjacobsen @uacjess @RolandBakerIII @TheSeeker268 @Daoyu15 @still_a_nerd @jjcouey @Harvard2H @ydeigin @CarltheChippy @ico_dna @Nomdeplumi1 @Real_Adam_B @nerdhaspower @scottburke777 @JJ2000426 @BahulikarRahul @alimhaider @antonioregalado @Ayjchan @R_H_Ebright @BretWeinstein @sanchak74 @JCalvertST [Thread] Addendum of WIV in Nature. What we know about the samples and the visits to the mineshaft of TG? TLDR: 7 trips: 4 already known (Ge et al., 2016), plus other 3 with massive sampling until 2015, including 7896-clade. All already in Latinne et al. (2020) #originsofSARSCoV2

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

@AntGDuarte @Rossana38510044 @jhouse678 @nature @edwardcholmes @arambaut @angie_rasmussen @K_G_Andersen What we know about the 7896 clade? Let's recap: 1/ They were published in June 2020 as part of a batch of 630 viruses of Latinne et. (2020). No details were given https://www.ncbi.nlm.nih.gov/nuccore/?term=MN312240%3AMN312869%5Baccn%5D

mn312240:mn312869[accn] - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

[Thread] New data on the Tongguan mine (Mojiang, Pu'er, Yunnan) where RaTG13 virus was collected in 2013. Previous threads: - Probable Location: - Probable outline: - Asahi expedition:

@franciscodeasis - Francisco de Asis

Do not miss the journalists race to the mine. I am sure that the CCP is more concerned that journalist take bat swabs rather than photographs...

@franciscodeasis - Francisco de Asis

@AP @Drinkwater5Reed Here the classification of the race of journalists to the Mojiang mine updated. It is becoming increasingly difficult. Not only car blocks, but also barricades. I can suggest alternative strategies (DM) #originsofSARSCoV2 #DRASTIC

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

[Compilation Thread] English translation of the MSc thesis of Xu Li (2013) and the PhD thesis of Canping Huang (2016). Files (non-pdf text format), with some additional info, upon request. Credits: both theses discovered by @TheSeeker268 #DRASTIC #originsofSARS2

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

[Thread] “SL3” the real name behind RaTG13 #originsofSARSCoV2 #DRASTIC

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

[Thread] RaTG13 amplicons names #originsofSARSCoV2 #DRASTIC TLDR: WIV inadvertently disclosed important info in fasta headers. Dates as 2017-2018 and a label pointing to Ra7896, a close virus to RaTG13 kept secret at the start of the outbreak and even today not fully disclosed

@franciscodeasis - Francisco de Asis

Just a single tweet, but WIV15 is critical enough. WHO team must request it asap!

@franciscodeasis - Francisco de Asis

@Rossana38510044 @luigi_warren @babarlelephant @AntGDuarte @MonaRahalkar @BillyBostickson @flavinkins @KevinMcH3 @DrAntoniSerraT1 @_coltseavers @rowanjacobsen @uacjess @RolandBakerIII @TheSeeker268 @Daoyu15 @still_a_nerd @jjcouey @Harvard2H @ydeigin @CarltheChippy @ico_dna @Nomdeplumi1 @Real_Adam_B @nerdhaspower @scottburke777 @JJ2000426 @BahulikarRahul @alimhaider @antonioregalado @Ayjchan @R_H_Ebright @BretWeinstein @sanchak74 @JCalvertST @PeterDaszak @TheSeeker 7/ Virus isolates at WIV First of all, "isolate" is an ambiguous word (https://t.co/bqEpZjhepA). Here I mean actual isolation. WIV used correlative numbering for them. WIV6 and WIV15 were never published. But WIV15 may perfectly come from 5-7th visit TG

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

[Thread] Complaints to top journals #originsofSARSCoV2 #DRASTIC Let’s start with a victory: the Addendum in Nature in November (not thanks to me that I am probably ignored, but thanks to some diligent scientists who pushed it, some of them from #DRASTIC)

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

[Thread] Pangolin CoV... or Bat CoV in pangolins samples? TLDR: A researcher of the team that sequenced the pangolins samples had taken samples in the mine of RaTG13 and in the place where RmYN02 was collected, also catched bats in Yunnan. Probably contaminated pangolin samples

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

[Thread] Unnoticed Lab contamination in the samples of first COVID-19 infected patients. 1/ Article: “There was a simultaneous undetected outbreak of Nipah in Wuhan” Explanation:

@franciscodeasis - Francisco de Asis

New theory: An infectious clone with a Ra4991-like backbone and a RBD from a hidden clade of the Mojiang mine could be a SARS-CoV-2 ancestor. Note: This theory does not exclude subsequent GoF

@franciscodeasis - Francisco de Asis

SARS-CoV-2 could come not from 1 virus of the Mojiang mine, but from 2 viruses of the mine! That is why we see strange things in the trees. RaTG13 & Ra7896 faked although not very far from reality. 1/ 2/ 3/

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

[Thread] In-silico molecular overclocking of RaTG13. TLDR: 191-nt RdRp segments of SARS-CoV-2, RaTG13 and Ra7896 show an unexpected molecular clock behavior. In-silico synonym mutations is one probable explanation.

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

[Thread] Missing / unpublished sequences WANTED: Live isolates WIV15 & WIV6; Ra7896 (complete genome); EPI_ISL_402122; MT394201

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

[Thread] New WIV theses found by @TheSeeker268. Let's make some comments on very important findings

@franciscodeasis - Francisco de Asis

WIV finally conceded on clade 7896: they acknowledged collecting them in the Mojiang mine and they published the complete genomes. Almost identical viruses (there is no diversity in the Mojiang mine, quite the opposite of that in Jinning).

@franciscodeasis - Francisco de Asis

New pre-print from WIV: RaTG15, which it seems it is former Ra7909 https://www.biorxiv.org/content/10.1101/2021.05.21.445091v1

Identification of a novel lineage bat SARS-related coronaviruses that use bat ACE2 receptor bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution biorxiv.org

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

[Thread] Bat tissue collection and cell lines from the 3rd trip to Tongguan (TG) mine in Mojiang in Apr-2013

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

[Thread] Regardless of the origin of the pandemic, it seems that the first superspreading event -in the Huanan seafood market- might not have been the result of chance.

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

[Thread] Necessary corrections to the China-WHO report. What they will probably fix and what they will not. TLDR: circular swap of 3 IPCAMS genomes + tampered onset of Wuchang accountant; First patients and first cluster; Some falsehoods in articles https://www.washingtonpost.com/world/asia_pacific/covid-wuhan-outbreak-who/2021/07/15/51e7e8a6-e2c6-11eb-88c5-4fd6382c47cb_story.html

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

[Thread] Who is the first known patient? There is a lot of confusion, so let's review all possible patients according published onset dates [of symptoms] up to 15-Dec-19.

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

Chen XX, Wuchang accountant and nephew of a doctor, 41 yrs old male (41M1), is not 1st known onset date on 08-Dec-19 as China-WHO report states (annex p. 117). His onset date was 16-Dec-19 according to 2 Wuhan Central Hospital doctors (Prof. Zhao Su & less-known hero Dr. Ai Fen)

@franciscodeasis - Francisco de Asis

No news on the 2nd batch of samples for the period 2014-2019

@franciscodeasis - Francisco de Asis

[Thread] Anthony et al. (2019) published a big database (discovered by @interne41914499) with viruses until Jun-2014. Kind of an Addendum of Anthony et al. (2017). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5467638/ There are interesting findings https://datadryad.org/stash/dataset/doi:10.5061/dryad.34db1gr

Global patterns in coronavirus diversity Since the emergence of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) and Middle East Respiratory Syndrom Coronavirus (MERS-CoV) it has become increasingly clear that bats are important reservoirs of CoVs. Despite this, only 6% of all CoV ... pmc.ncbi.nlm.nih.gov
Dryad | Data -- Global patterns in coronavirus diversity datadryad.org

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

[Thread] The mystery of “live isolate” Rs4874 with no WIV number assigned. TLDR: Probably not a [pure] live isolate but a mere infectious clone. Just another case of academic cheating, to make sure the article was accepted by PLoS Pathog (Hu et al., 2017)

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

[Thread] FOIA from @USRightToKnow regarding Latinne et al. (2020) and clade 7896 TLDR: No sequence was deleted/modified since Aug-2019, but it seems they wanted to buy time for not publishing the viruses very early in the pandemic. https://usrtk.org/biohazards/foi-documents-on-origins-of-sars-cov-2-risks-of-gain-of-function-research-and-biosafety-labs/

FOI documents on origins of Covid-19, gain-of-function research and biolabs Freedom of information documents from our investigation into the origins of Covid-19, gain-of-function and biolab risks. usrtk.org

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

[Thread] New unforced error. GIABR, the lab of the pangolins, has just uploaded sequences (MW600658:MW600715) that shows a trip to the Mojiang mineshaft or nearby on 22-Aug-2017, well after the last known trip of WIV in 2015. Libiao Zhang explicitly credited as collector.

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

[Thread] Nth falsehood from WIV/EHA. The Addendum acknowledged only sampling bats "once or twice a year" in Mojiang, but this EHA report shows at least 4 trips in 2014. Latinne et al. (2020) did not publish anything from "Jul.--Sep. 2014" & Mojiang.

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

[Thread] Wang et al. (2021): Another article revealing trips to the Mojiang mine and Laos was kept in the limbo when the pandemic started. Note: The article covers HKU10 viruses which are not related with SARS-like viruses. https://www.mdpi.com/1999-4915/13/10/1962

Genomic Characterization of Diverse Bat Coronavirus HKU10 in Hipposideros Bats Bats have been identified as natural reservoirs of a variety of coronaviruses. They harbor at least 19 of the 33 defined species of alpha- and betacoronaviruses. Previously, the bat coronavirus HKU10 was found in two bat species of different suborders, Rousettus leschenaultia and Hipposideros pomona, in south China. However, its geographic distribution and evolution history are not fully investigated. Here, we screened this viral species by a nested reverse transcriptase PCR in our archived samples collected over 10 years from 25 provinces of China and one province of Laos. From 8004 bat fecal samples, 26 were found to be positive for bat coronavirus HKU10 (BtCoV HKU10). New habitats of BtCoV HKU10 were found in the Yunnan, Guangxi, and Hainan Provinces of China, and Louang Namtha Province in Laos. In addition to H. pomona, BtCoV HKU10 variants were found circulating in Aselliscus stoliczkanus and Hipposideros larvatus. We sequenced full-length genomes of 17 newly discovered BtCoV HKU10 strains and compared them with previously published sequences. Our results revealed a much higher genetic diversity of BtCoV HKU10, particularly in spike genes and accessory genes. Besides the two previously reported lineages, we found six novel lineages in their new habitats, three of which were located in Yunnan province. The genotypes of these viruses are closely related to sampling locations based on polyproteins, and correlated to bat species based on spike genes. Combining phylogenetic analysis, selective pressure, and molecular-clock calculation, we demonstrated that Yunnan bats harbor a gene pool of BtCoV HKU10, with H. pomona as a natural reservoir. The cell tropism test using spike-pseudotyped lentivirus system showed that BtCoV HKU10 could enter cells from human and bat, suggesting a potential interspecies spillover. Continuous studies on these bat coronaviruses will expand our understanding of the evolution and genetic diversity of coronaviruses, and provide a prewarning of potential zoonotic diseases from bats. mdpi.com

@franciscodeasis - Francisco de Asis

@franciscodeasis - Francisco de Asis

Little is being said about this study of Qi Jin (IPBCAMS) that reveals how all the SARS lineages of the Mojiang mine have become extinct. And they have been looking for viruses at 9 sites in Mojiang County or very close. https://www.researchsquare.com/article/rs-885194/v1

A comprehensive survey of bat sarbecoviruses across China for the origin tracing of SARS-CoV and SARS-CoV-2 Severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2 have been thought to originate from bat, but whether the cross-species transmission occurred directly from bat to human or through an intermediate host remains elusive. In this study, we performed CoV screening of 102 samples... researchsquare.com

@franciscodeasis - Francisco de Asis

Layouts of the Huanan seafood market (West building): https://t.co/XouISuw5gg

@franciscodeasis - Francisco de Asis

In the most important part of their heatmap (SW corner) they only put 3 stalls (all misplaced) and they omit the toilets and the Mahjong room. Their response to our raising issues?: silence and blocks. It seems we are making essential contributions. Again.

@franciscodeasis - Francisco de Asis

Select Committee's data leak within pdf https://t.co/lLHg7xMzwF

@franciscodeasis - Francisco de Asis

Let's go on an Easter egg hunt inside the pdf 🧵

@franciscodeasis - Francisco de Asis

https://t.co/bXa3RzGLrO

@franciscodeasis - Francisco de Asis

🧵Natural and non-natural live isolates (WIV & related) TLDR: - A substantial portion of the research remains unpublished. - Missing live isolate WIV15. - No samples, isolates, chimeric and MA viruses in 2017-2019? - What is "Rs4874" really? https://t.co/25XbQLCQQb https://t.co/9JPusWbl9l

Saved - December 20, 2024 at 8:25 PM
reSee.it AI Summary
I’ve been diving into the complex world of pangolins, particularly focusing on the involvement of key figures like Yang Ruifu and Yigang Tong in bioweapons research and the mysterious "pangoscam." It appears that pangolins smuggled from Southeast Asia may carry viruses related to SARS-CoV-2, raising questions about their role in the pandemic. There are concerns about a cover-up involving the PLA and various agencies regarding the handling of these animals and the viruses they carry. More revelations are expected as I continue to explore this intricate narrative.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Tip from the Monkey Pangolins, September 2019 and PLA are the key to this mystery Stay Tuned!

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

1. Yang Ruifu

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Meet Yang Ruifu, CCP's biological weapons expert https://gnews.org/zh-hans/413491/ via @Gnews202064 Interesting expose of China's top bioweapons expert who oversaw fake pangolin research Paper 1: https://wenku.baidu.com/view/2453b5ce05087632311212e1.html Paper 2: https://wenku.baidu.com/view/a093142258fb770bf78a55e6.html# Pangolin https://news.sina.com.cn/c/2020-02-07/doc-iimxyqvz0871821.shtml

华南农业大学发现穿山甲为新冠病毒潜在中间宿主 原标题:华南农业大学发现穿山甲为新型冠状病毒潜在中间宿主2月7日凌晨1时许,华南农业大学在其官方微信宣布:华南农业大学、岭南现代农业科学与技术广东省实验室沈永义教授、肖立华教授等科研人员联合中国人民解放军军事科学院军事医学研究院杨瑞馥研究员 news.sina.com.cn

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

2. A jacobin capuchin dangling a flagellin pangolin on a javelin while playing a mandolin and strangling a mannequin on a paladin's palanquin, said Saladin More to come tomorrow!

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

3. Yigang Tong https://tongyigang.tripod.com/mycv-en.html Archived: https://archive.ph/8CROj

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

4. YT Interview Some bats & pangolins carry viruses related with SARS-CoV-2, found in SE Asia and in Yunnan, & the pangolins carrying SARS-CoV-2 related viruses were smuggled from SE Asia, so there is a possibility that SARS-CoV-2 were coming from there. https://news.cgtn.com/news/2021-03-31/VHJhbnNjcmlwdDUzMzI0/index.html

CGTN | Breaking News, China News, World News and Video Get the news as it breaks and go behind the day's top stories, from politics, business, culture and sports to nature, travel and technology. CGTN delivers a Chinese perspective on global news through innovative use of the latest media tools. news.cgtn.com

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

5. Yigang Tong and Ecohealth Alliance https://www.ecohealthalliance.org/wp-content/uploads/2018/04/Nature-SADS-CoV.pdf

Page not found - EcoHealth Alliance ecohealthalliance.org

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

6. “Therefore, we feel that it is necessary to conduct the study of virus source under a global framework,” said Tong Yigang, the animal and environment group leader on the Chinese team. Liang called accusations that China did not share data “invalid.” https://abcnews.go.com/Health/wireStory/china-pushes-expand-virus-origin-search-border-76787763

ABC News – Breaking News, Latest News and Videos Page Unavailable abcnews.go.com

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Pangolin Girl Thanks All the Help with Javan Pangolins https://cbcgdf.wordpress.com/2019/07/18/the-pangolin-girl-thanks-all-the-help-with-the-javan-pangolin-always-sleep-and-last-night-rescue-and-rehabilitation-working/

The Pangolin Girl Thanks All the Help with the Javan Pangolin “Always Sleep” and “Last Night” Rescue and Rehabilitation Working On April 18th, the China Biodiversity Conservation and … cbcgdf.wordpress.com

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Dead tired! To be continued!

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Supplementary Data from @Daoyu15

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Interesting work by @himalaya_de The "3 kings" of military medicine: Tong Yigang, Wu-Chun Cao, Yang Ruifu Former Director of Institute of Microbiology & Epidemiology of Academy of Military Medical Sciences Why are they so involved with the "Pangoscam"?

@Aufklaerung2021 - Libertas perfundet omnia luce

军医科三毒王:童贻刚(另外两个:曹务春、杨瑞馥) 曾任军事医学科学院微生物流行病研究所主任 WHO新冠溯源中方专家组动物与环境组组长 冠状病毒研究网络连连看:CDC高福,军医科曹务春,香港大学管轶袁国勇梁卓伟,武毒所石正丽周鹏,新加坡王林发,美国Peter Daszak, 澳洲Edwards Holmes 🦅🦅🦅

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

More details on Yang Ruifu again thanks to @himalaya_de His publication record: ghb.jlu.edu.cn/info/1096/1239… Archived: https://archive.ph/XWBdD He revealed that the Malayan Pangolin samples were obtained from a "secret agency" not from the Customs Department... More on that soon.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

How the cover up and "pangoscam" was developed with the involvement of the PLA's Academy of Military Science Tianjin and SCAU and GIABR.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Link for above: https://news.sina.com.cn/c/2020-02-07/doc-iimxyqvz0871821.shtml Archived: https://archive.ph/T4kHm

华南农业大学发现穿山甲为新冠病毒潜在中间宿主 原标题:华南农业大学发现穿山甲为新型冠状病毒潜在中间宿主2月7日凌晨1时许,华南农业大学在其官方微信宣布:华南农业大学、岭南现代农业科学与技术广东省实验室沈永义教授、肖立华教授等科研人员联合中国人民解放军军事科学院军事医学研究院杨瑞馥研究员 news.sina.com.cn

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Interlude - GIVE ME BACK MY SCALES! https://www.youtube.com/watch?v=VnV3fhtdsB8

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

"The authors found 6 complete or partial genome sequences of coronaviruses in 2017-2018 from smuggling pangolin samples in Guangxi. These viruses share 85.5% nucleotide sequence homology with SARS-CoV-2" https://english.buct.edu.cn/news1/132493.htm What did they do with them?

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

With low or no pathogenicity in humans & close genetic relationship with 2019-nCoV, our pangolin coronavirus provides an ideal alternative model for 2019-nCoV research. Our reasoning of this having low or no pathogenicity in humans was based on the fact that, back in 2017.....

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

"....no suspected infections were found in those having close contacts with pangolins; and our pangolin coronavirus isolate was **** routinely cultured in biosafety level 2 facilities ****" https://journals.lww.com/cmj/fulltext/2020/05050/repurposing_of_clinically_approved_drugs_for.8.aspx

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

"Here we present a screening of clinically approved drugs for anti-coronavirus activity in this 2019-nCoVr model, & identify potent inhibitors for pangolin coronavirus infection. CEP, selamectin, and mefloquine hydrochloride, potential drugs for treating 2019-nCoV infection."

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

"Our results strongly suggest that cepharanthine (CEP) is a wide-spectrum inhibitor of pan-betacoronavirus, and further study of CEP for treatment of 2019-nCoV infection is warranted" https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7147283/

Repurposing of clinically approved drugs for treatment of coronavirus disease 2019 in a 2019-novel coronavirus-related coronavirus model Medicines for the treatment of 2019-novel coronavirus (2019-nCoV) infections are urgently needed. However, drug screening using live 2019-nCoV requires high-level biosafety facilities, which imposes an obstacle for those institutions without such ... pmc.ncbi.nlm.nih.gov

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

So far we find that they experimented on these GD & GX Pangolin viruses at many BSL2 level research institutes since 2018, including ones involved in Yunnan bat sampling expeditions and isolation of Bat Betacovs, also looking into pan betacoronavirus treatments. Interesting!

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

But all is not as it seems in Pango Land... https://cbcgdf.wordpress.com/2020/02/10/department-of-forestry-of-guangxi-zhuang-autonomous-region-pangolin-is-a-transmission-channel-of-2019-cov-why-not-report-that-the-pangolin-was-found-to-be-highly-pathogenic-in-2018/

Department of Forestry of Guangxi Zhuang Autonomous Region: Pangolin is a transmission channel of 2019-CoV? Why not report that the pangolin was found to be highly pathogenic in 2018? Nowadays, the South China Agricultural University and o… cbcgdf.wordpress.com

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

"After finding the highly pathogenic virus, the Department of Forestry of Guangxi didn’t take any reporting and warning measures, instead of it, under different names, they continue to distribute pangolins to various cooperative scientific research units"

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

".... which creates conditions for possible virus transmission. So, is the Center of Rescue related to the outbreak of 2019–CoV?" It is recommended that relevant departments immediately file a case for investigation. Archived: https://archive.ph/cvKmo

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Team of Professor Zhu, director of Chinese Pangolin Research Center, the team of Dean Wu of Lide Animal Hospital, several local South China Agricultural University volunteers, Malaysian professor Zhu from China Pangolin Research Center cbcgdf.org/NewsShow/4857/… @Daoyu15 @coroldo1

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

"You have given pangolins many times to iron & steel companies, Chinese medicine experts, etc. And we, the China Biodiversity Conservation Agency, ask you for a pile of shit & pangolin shit. You make things difficult & you will not give it to this day." https://archive.ph/yEILP

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

1 "Most of the imported pangolins carry highly pathogenic viruses caused by human-induced decline in their resistance" 2 "the testing agency was the Chinese military. CAS Institute of Microbiology & Epidemiology has not yet provided the inspection report" https://archive.ph/b1nPT

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

What was the PLA doing testing these Pangolins? Why the big interest? Why didn't they release the results to the Guangxi Forestry Department? Maybe someone can ask Colonel Wu Chun-Cao? @dezying

@dezying - Ying☮️

"Beijing Institute of Biotechnology", "Beijing Institute of Microbiology and Epidemiology"? Most published papers refrain to mention their military affiliations: Academy of Military Medical Sciences (AMMS) of PLA Directors of them, Wei Chen and Wuchun Cao are PLA generals.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Counterfeit PLA? Counterfeit Viruses? Did it detect such a major thing as a "highly pathogenic virus"? Why did the Chinese Academy of Military Sciences not give Guangxi Forestry Bureau any voucher materials? How did they know that it was a 'parvovirus'?" https://archive.ph/b1nPT

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

or we could ask Eddie's Buddy, Yigang "PLA" Tong Beijing Institute of Microbiology and Epidemiology State Key Laboratory of Pathogen and Biosecurity https://www.researchgate.net/lab/Yigang-Tong-Lab If he's not too busy rubbing 75% Ethanol on Nipples... https://www.researchgate.net/publication/343735801_The_effect_of_whey_protein_on_viral_infection_and_replication_of_SARS-CoV-2_and_pangolin_coronavirus_in_vitro

ResearchGate - Temporarily Unavailable researchgate.net
ResearchGate - Temporarily Unavailable researchgate.net

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

More PLA Pangolin Shenanigans? Maybe they died from parvovirus, maybe not Parvovirus can infect pangolins https://www.researchgate.net/publication/338237798_Fatal_Canine_Parvovirus-2_CPV-2_Infection_in_a_Rescued_Free-ranging_Taiwanese_Pangolin_Manis_pentadactyla_pentadactyla The inspection agency is the Institute of Microbiology and Epidemiology of the Chinese Academy of Military Sciences.

ResearchGate - Temporarily Unavailable researchgate.net

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

From #DRASTIC Research & @CBCGDF_China 1. GX “virus positive” pangolin samples were investigated by PLA's Institute of Microbiology & Epidemiology (Beijing) in 2018. 2. Yang Ruifu revealed that GD samples were obtained from a "secret agency" (特殊部门) NOT from Customs (是海关)!

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

I believe you are not unfamiliar with the series of stories triggered by the Green Club and Guangxi Forestry Department on the information disclosure and rescue of 34 pangolins seized on August 17, 2017? ...Even if you are familiar, better to read this: https://archive.ph/jS0uE

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

On August 21, 2017, the Coast Guard of the Guangxi Zhuang Autonomous Region seized a suspected case of illegally transporting endangered wild animals. 33 live pangolins and 1 dead pangolin were seized & they were handed over to Guangxi Forestry Department https://archive.ph/0nCQY#selection-347.0-347.356

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Then there were 34 dead Pangolins.... The Guangxi Forestry Department Cover Up Exposed https://www.thepaper.cn/newsDetail_forward_2311838 For the death of 33 pangolins, the Green Club will definitely investigate them to the end! https://archive.ph/KqYCd#selection-501.0-501.50 as will #DRASTIC for other motives...

国家林草局撤销广西林业厅涉穿山甲回复:主要事实认定不清_绿政公署_澎湃新闻-The Paper 中国绿发会此前就广西林业厅救护的30多只穿山甲相关情况提出信息公开申请,广西林业厅回复称,相关信息属于咨询性质,不属于政府信息。 thepaper.cn

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Some anomalies in their cover up story? Which agency issued the test report? Who are the experts involved in the test? What kind of virus is the detected virus? What are the results of the cooperative research? https://archive.ph/jKZca https://t.co/bgUJ1pbm58

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

More good questions Which research group of the Chinese Academy of Sciences and the Chinese Academy of Military Sciences conducted the research? How much is the research funding? What role did the so-called "highly pathogenic virus" play in the death of pangolins?

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Some Answers about the dead Guangxi Pangolins (2017) on August 17, 2017, the Qinzhou Coastal Police handed over 34 smuggled Malay pangolins of which 32 were live and 2 were dead. The 32 still fell ill and died. On October 22, 2017, the last pangolin died https://archive.ph/pHLGP https://t.co/2XrG8mzQl4

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

"Another new coronavirus that discovered long ago" Our friend, Tong Yigang at the Institute of Microbiology and Epidemiology, had successfully isolated the Guangxi Pangolin Virus GX_P2V in 2017 med.china.com.cn/content/pid/16… https://t.co/aPzgchqswt

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

"In 2017, we found a new type of coronavirus in a batch of smuggled wild pangolins in Guangxi & isolated it. After the sequence of the new coronavirus was announced, we found that the pangolin coronavirus was compared with the new coronavirus" https://archive.ph/pDECd#selection-269.0-277.428

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Tong Yigang said: 1. The homology of the virus is relatively high 2. This virus is named GX_P2V 3. *Research has found that it has no obvious pathogenicity to humans* What Research, Professor Tong Yigang? When was this research carried out? Was it ever published? Where and Why? https://t.co/oV0fIT4KB7

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Will someone ask him these questions? Professor Tong Yigang has long been engaged in the high-throughput detection & identification of pathogens He has participated in the treatment of major national & military infectious disease epidemics for a long time tonglab.cc/?page_id=138 https://t.co/ZaVSKaTG4r

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Introduction of Professor Tong Yigang https://archive.ph/osaf2 https://t.co/uaJGI0V9Ti

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

In 2017 the team successfully isolated GX_P2V. Tong Yigang recalled that the virus had no clear infectivity to humans “At that time, I just thought that a new virus was discovered, but I didn’t know what it was useful for, so I didn’t publish an article” https://archive.ph/ZYdbU#selection-543.0-571.263 https://t.co/CsaoYLGmcX

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Haven't we heard that line before somewhere? Oh yes, Peter Daszak talking about RaTG13: Didn't think it was important, errrr, we just chucked it in the freezer, innit, never even looked at it again... Daszak lied about that. Did Tong Yigang lie about GX_P2V? https://t.co/FN7dtmFiKX

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Funnily enough both Peter Daszak and Tong Yigang helped write the recent doctored WHO Report... https://t.co/pVoH9RsFuN Document: Annexes. "Animal and environment: Tong Yigang*" p. 25-26 *Subgroup lead "Animal and environment: Peter Daszak*" p. 27 Thanks @FabienColombo

@FabienColombo - Fabien Colombo

@BillyBostickson Thank you so much for this thread, Billy. I guess "international community" might have some questions to Yong Yigang, as he has been the leader of the subgroup of "Animal & Environnement". PS: His "international counterpart" was Peter Daszak. 🌎🌏🌍🤝🙏 https://t.co/5bzKA3vzjw

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Coincidences? Isn't it quite strange that 2 of the closest relatives to SARS-COV-2 in different ways, RaTG13 & GX-PV2 were isolated & kept in Chinese Labs? And Two Doctors very intimate with them worked together on the doctored WHO report? One linked to the PLA, the other to DTRA https://t.co/p0OKasxtKz

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

End of Part 1 - Pangolin Shenanigans unroll @threadreaderapp @buzz_chronicles save https://t.co/OeUfzpvOuR

Saved - December 15, 2024 at 7:19 PM
reSee.it AI Summary
I’ve been exploring the potential threats posed by state-sponsored biological attacks, particularly focusing on China's biological warfare capabilities. My research includes various studies, including a Master's thesis and analyses by experts like Dany Shoham. I’ve delved into the Wuhan Institute's activities, the use of primates in research, and historical context, drawing parallels to past U.S. operations. This investigation aims to uncover the logistics and implications of biological warfare research in China, acknowledging that the findings could lead to significant insights or remain inconclusive.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

An Examination of the Potential Threat of a State-Sponsored Biological Attack Against the United States 2019 Master's Thesis https://bearworks.missouristate.edu/cgi/viewcontent.cgi?article=4366&context=theses…

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

This section refers to: China’s Biological Warfare Programme: An Integrative Study with Special Reference to Biological Weapons Capabilities (2015) by Danny Shoham https://idsa.in/jds/9_2_2015_ChinasBiologicalWarfareProgramme PDF https://idsa.in/system/files/jds/jds_9_2_2015_DanyShoham.pdf

Page not found - IDSA idsa.in
Page not found - IDSA idsa.in

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

The author of the Master's Thesis, Corey Pfluke wrote a summarised version of her section on China in 2020: Biohazard: A Look at China’s Biological Capabilities and the Recent Coronavirus Outbreak https://www.airuniversity.af.edu/Wild-Blue-Yonder/Article-Display/Article/2094603/biohazard-a-look%20-at-chinas-biological-capabilities-and-the-recent-coronavirus-o/

Biohazard: A Look at China’s Biological Capabilities and the Recent Coronavirus Outbreak Many have speculated that the coronavirus outbreak that begin in China in 2019 could have been an unintentional consequence of alleged bioweapon research in Wuhan. This article will look into the airuniversity.af.edu

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

"Although assessing China’s military capabilities is notoriously difficult due to the secrecy & opacity its institutions maintain, a combination of historical records, assessments, & studies provide a view of China’s BW programme, this view is unsettling" https://www.orfonline.org/expert-speak/a-look-at-chinas-biowarfare-ambitions/

A look at China’s biowarfare ambitions A look at China’s biowarfare ambitions orfonline.org

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

An Indian Perspective (Part 1) AN ASSESSMENT OF CHINA’S BIOLOGICAL WARFARE CAPABILITIES AND NEED FOR GLOBAL APPROACH TO BIO-SECURITY https://www.researchgate.net/publication/341354904_AN_ASSESSMENT_OF_CHINA'S_BIOLOGICAL_WARFARE_CAPABILITIES_AND_NEED_FOR_GLOBAL_APPROACH_TO_BIO-SECURITY_Part_1

ResearchGate - Temporarily Unavailable researchgate.net

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

An Indian Perspective (Part 2) AN ASSESSMENT OF CHINA’S BIOLOGICAL WARFARE CAPABILITIES AND NEED FOR GLOBAL APPROACH TO BIO-SECURITY https://www.researchgate.net/publication/341359810_AN_ASSESSMENT_OF_CHINA'S_BIOLOGICAL_WARFARE_CAPABILITIES_AND_NEED_FOR_GLOBAL_APPROACH_TO_BIO-SECURITY_-_Part_2

ResearchGate - Temporarily Unavailable researchgate.net

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

My thread on China's BW Program

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

It was claimed some time ago that there were strange goings on in Wuhan https://www.nonproliferation.org/wp-content/uploads/npr/91crod.pdf

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

An important Reference is a paper by Croddy (2002 ) (a little dated but with many useful references to suspected facilities) including the Wuhan Institute of Biological Products (WIBP..next door to the WIV BSL4 Complex in Jingxia) https://www.nonproliferation.org/wp-content/uploads/npr/91crod.pdf

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Hence my tweet yesterday and "126 tedious WIBP supply documents translated A minor victory!"

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Weekend at #DRASTIC Yandex Translation factory WIBP purchased 23 ordinary monkey cages for quarantine observation and immunizations, and for the experiment the monkey proceeds to ABSL-3 Containing cage, cage, manger, water bottles, shit tray, etc.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Essential Background Reading on WIBP Published by DRASTIC a year ago https://www.researchgate.net/publication/350485858_Wuhan_Institute_of_Biological_Products_Co

ResearchGate - Temporarily Unavailable researchgate.net

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Referenced in the Taiwan News by @keverington https://www.taiwannews.com.tw/en/news/4137284

Biowarfare history of Chinas Sinopharm vaccine maker raises questions | Taiwan News | Mar. 2, 2021 16:48 taiwannews.com.tw

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Another article by Dany Shoham focusing on the Canadian-Tianjin Angle: China's Biological Warfare Programme and the Curious Case of Dr. Xiangguo Qiu (2019) https://idsa.in/cbwmagazine/chinas-biological-warfare-programme

China’s Biological Warfare Programme: An Integrative Study with Special Reference to Biological Weapons Capabilities - IDSA This study attempts to profile China’s biological warfare programme (BWP), with special reference to biological weapons (BW) capabilities that exist in facilities affiliated with the defence establishment and the military.… Continue reading China’s Biological Warfare Programme: An Integrative Study with Special Reference to Biological Weapons Capabilities idsa.in

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Dany Shoham's concise analysis of Xiangguo Qiu's Case China's Biological Warfare Programme and the Curious Case of Dr. Xiangguo Qiu | Manohar Parrikar Institute for Defence Studies and Analyses (2019) https://idsa.in/cbwmagazine/chinas-biological-warfare-programme#.YBVMC2fhSiw.twitter

China’s Biological Warfare Programme: An Integrative Study with Special Reference to Biological Weapons Capabilities - IDSA This study attempts to profile China’s biological warfare programme (BWP), with special reference to biological weapons (BW) capabilities that exist in facilities affiliated with the defence establishment and the military.… Continue reading China’s Biological Warfare Programme: An Integrative Study with Special Reference to Biological Weapons Capabilities idsa.in

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

The Radosavljevic–Belojevic method for outbreak scoring & differentiation shows that the official assumption of its natural origin is questionable and highlights the probability that the pathogen may have been "accidentally" introduced to humans https://www.researchgate.net/publication/352800586_Analysis_of_COVID-19_outbreak_origin_in_China_in_2019_using_differentiation_method_for_unusual_epidemiological_events

ResearchGate - Temporarily Unavailable researchgate.net

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

“The genomic origin of the strain that infected patient zero was a Chinese bat virus that underwent extensive pre-adaptation to humans, including continual transmissibility.. “The open question is how, where, and when such exceptional genomic pre-adaptation took place”

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

One possibility is that a wild-type virus was 1st propagated repeatedly in human tissue cultures & the resulting spontaneously upgraded virus was then used to experimentally infect monkeys or ferrets—one of which then accidentally infected staff in the lab https://www.jns.org/opinion/where-did-covid-19-really-come-from/

Where did COVID-19 really come from? - JNS.org The true genomic origin and direct source of SARS-CoV-2 are hotly debated and highly consequential. Both science and intelligence will contribute to uncovering the evidence needed to answer these questions conclusively. jns.org

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

An interesting observation "The WIV has long been routinely supplied with rhesus monkeys from the Macaque Breeding Base in Suizhou City" Note the references have been deleted: safeboxasia.com/en/center/inde… and safeboxasia.com/en/news/info_9… and replaced with chinese lottery website!

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

However, for once, google cache saves the day webcache.googleusercontent.com/search?q=cache… webcache.googleusercontent.com/search?q=cache… webcache.googleusercontent.com/search?q=cache… webcache.googleusercontent.com/search?q=cache… webcache.googleusercontent.com/search?q=cache… webcache.googleusercontent.com/search?q=cache…

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Top Gene Bio's new cleaned website http://topgenebio.com/ Facebook Page: https://www.facebook.com/topgenebio/ Invite you to search

湖北天勤生物科技股份有限公司 湖北天勤生物科技股份有限公司 topgenebio.com
Hubei Topgene Biotechnology Co.,Ltd | Wuhan Hubei Topgene Biotechnology Co.,Ltd, Wuhan, China. 56 likes. Pre-clinical safety assessment facebook.com

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Snapshots

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

20,000 Caged rats and mice 800 + Monkeys

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

1300 Types of genetically engineered mice 300 types of genetically engineered rats

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

A well known supplier of Monkeys....

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

"Business Segment"

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Video https://www.facebook.com/topgenebio/videos/330622824279165

Hubei Topgene Biotechnology Co.,Ltd | Facebook facebook.com

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Taking a break, to be continued.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

The "chinese lottery" website can actually be accessed in all its tedious glory by installing a noscript extensión safeboxasia.com/cn/animal/list…

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

You may be wondering why this thread has diverged into Chinese monkey breeding minutia... Reason being that any putative Chinese BW program would require monkeys for experiments, as was the case in USA & UK (Operation Cauldron) https://en.wikipedia.org/wiki/Operation_Cauldron https://www.bbc.com/news/uk-england-wiltshire-11549809

Operation cauldron - Wikipedia en.wikipedia.org
Porton Down film shows Cold War plague tests on animals Film footage showing a controversial Cold War experiment exposing animals to plague bombs is revealed. bbc.com

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Operation Cauldron Film: The trials were conducted by scientists from Porton Down in Wiltshire. About 3,500 monkeys and guinea pigs were killed after exposure to "plague bombs" https://archive.org/details/OperationCauldron1952 and https://vimeo.com/50042014

Internet Archive: ErrorHamburger iconInternet Archive logoWeb iconTexts iconVideo iconAudio iconSoftware iconImages iconDonate iconEllipses iconDonate iconUser iconUpload iconSearch iconSearch iconSearch iconDonate icon archive.org
Operation CAULDRON 1952 Warning! This declassified film, Operation CAULDRON 1952, contains footage which some viewers may find upsetting. In 2008, the Ministry of Defence made it clear… vimeo.com

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Operation Cauldron, The Stuff of Monkey Nightmares More Chinese Monkey Research and Breeding Programs to come tomorrow

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

5 species of primates are used in China for biomedical research 2 species, crab-eating macaque & common marmoset are alien 3 species, pigtail, stumptail & rhesus macaques, are indigenous Those used for biomedical research are crab-eating & rhesus macaques https://www.ncbi.nlm.nih.gov/books/NBK221790/

Chinese Primate Status and Primate Captive Breeding for Biomedical Research in China Nineteen species of primates are distributed in China, including three families and six genera (Table 1). There are perhaps 21 species of Taiwan macaque (Macaca cyclopis, only distributed in Taiwan with 7000 individuals) and douc langur (Pygathrix nemaeus, only recorded in Hainan in 1892) are counted. Loris (Nycticebus spp.) and gibbons (Hylobates spp.) mainly range in Yunnan, with some in Guangxi and Hainan. Langurs (Presbytis spp. and Trachypithecus spp.) are mainly in Southwest China and Guangxi. Snub-nosed monkeys (Pygathrix spp.) are in Southwest China, with some in Gansu, Shaanxi, and Hubei. The species of macaques have wide distribution in China and range mostly south of Yellow River. The stumptail macaque (Macaca arctoides) is in Yunnan, Guangxi, Guizhou, Jiangxi, Hunan, Guangdong, and Fujian; the Assamese macaque (Macaca assamensis) in Yunnan, Guangxi, and Tibet; the pigtail macaque (Macaca nemestrina) in Yunnan; the Tibetan macaque (Macaca thibetana) is endemic to China and in Southwest and Middle China, southern parts of Gansu, and Shaanxi, Guangxi, Fujian, and Zhejiang; and the rhesus macaque (Macaca mulatta) has the widest distribution in China and is mostly in the areas to the south of Yellow River but mainly in the southern part of China. ncbi.nlm.nih.gov

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

The following information on monkey breeding centres in China is from 2001. There have been some changes... For example, Hubei Topgene Biotechnology, the new supplier to WIV and WIBP was not listed 20 years ago.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Tables in previous tweet are from Chinese Primate Status and Primate Captive Breeding for Biomedical Research in China https://www.ncbi.nlm.nih.gov/books/NBK221790/table/ttt00004/?report=objectonly

TABLE 2, The Captive Breeding and the Exportation of Rhesus Macaque (Macaca mulatta) and Crab-eating Macaque (Macaca fascicularis) for Biomedical Research in China in 2001a - International Perspectives - NCBI Bookshelf ncbi.nlm.nih.gov

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

From 2004, another table offers more information Highlighted is Laboratory Animal Center of Chinese Academy of Military Medical Sciences in Beijing From: Notice of State Forestry Administration on Strengthening the Management of Experimental Monkeys https://archive.ph/fIzFx https://t.co/hQ2GBrJXg5

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

To be continued..Homework China has become the Mecca for those deluded Chinese & Foreign Researchers keen to abuse monkeys on the altar of Science. While author Xin Hao gloats over low labor costs Monkey Research in China Developing a Natural Resource https://www.cell.com/fulltext/S0092-8674(07)00726-X https://t.co/X8HreLeEhg

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Please Note This thread is an investigation of the breeding centres, laboratory research evidence, logistics and locations of possible Biological Warfare research in China using non human primates. It may lead nowhere or it might lead somewhere, that remains to be determined. https://t.co/xUQBpqIIhr

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

unroll @threadreaderapp save @rattibha @reSeeIt save thread save @buzz_chronicles

Saved - December 17, 2023 at 9:59 AM

@gdemaneuf - Gilles Demaneuf

1/ Here, I will expose a well organised and very questionable framing of the SARS2 origins. The core of the story is the drafting in February 2020 of a commentary denying any possible man-made virus. It is not often discussed, but is very revealing. https://www.tandfonline.com/doi/full/10.1080/22221751.2020.1733440?scroll=top&needAccess=true https://t.co/r10CbdXduW

Saved - December 23, 2024 at 8:23 AM
reSee.it AI Summary
I've been compiling information on early COVID-19 cases, focusing on various threads. I discussed Connor Reed, an early case from the UK, and explored the origins of the outbreak, including market and non-market cases. I addressed misconceptions in published research and highlighted significant research grants related to the virus. Additionally, I examined the connections between early cases and the Huanan Seafood Market, arguing against it being the pandemic's origin. I also provided updates on specific cases and WIV personnel involved in research.

@Engineer2The - TheEngineer2 🇨🇦

1/ Cleanup day. A summary of threads for things I've worked on in no particular order.

@Engineer2The - TheEngineer2 🇨🇦

2/ The Connor Reed early case

@Engineer2The - TheEngineer2 🇨🇦

1. The search for cluster zero continues. Connor Reed (from UK - age 25) was an English teacher in Wuhan. He's an early case, developing symptoms on Nov 25. https://www.dailymail.co.uk/news/article-8075633/First-British-victim-25-describes-coronavirus.html

First British victim, 25, describes coronavirus Connor Reed, a 25-year-old expat from Llandudno in North Wales, has worked in a school in Wuhan, China, for almost a year and was the first man infected with the coronavirus in November. dailymail.co.uk

@Engineer2The - TheEngineer2 🇨🇦

3/ The mahjong and early market cases

@Engineer2The - TheEngineer2 🇨🇦

1/🧵on the earliest market cases and an exploration into where the market outbreak began. First, a summary of the situation highlighted in purple as of Dec 13, 2019. #RacoonDogGate

@Engineer2The - TheEngineer2 🇨🇦

4/ The early official cases outside the market

@Engineer2The - TheEngineer2 🇨🇦

In my earlier thread, I probably should have covered the NON market cases as well on the right.

@Engineer2The - TheEngineer2 🇨🇦

5/ The unofficial early cases before the market outbreak

@Engineer2The - TheEngineer2 🇨🇦

1/🧵on the "unofficial" early Hubei SARS-SoV-2 cases. This has been covered before, but intend to add my two bits near the end. For clarity, I include only China cases here, but admit there may be others. I covered the official cases in this other thread.

@Engineer2The - TheEngineer2 🇨🇦

6/ Some evidence that parts of the DARPA grant were carried out by the Chinese without DARPA funding

@Engineer2The - TheEngineer2 🇨🇦

1/ Some have suggested that since the DARPA grant was rejected, the work wasn't done. This is not necessarily the case, as some DEFUSE proposed work was in progress or already done. 🧵on what work proposed in DEFUSE was carried out. https://drasticresearch.files.wordpress.com/2021/09/main-document-preempt-volume-1-no-ess-hr00118s0017-ecohealth-alliance.pdf

@Engineer2The - TheEngineer2 🇨🇦

7/ Showing the earliest official case is not related to the Huanan Seafood Market

@Engineer2The - TheEngineer2 🇨🇦

1/🧵showing that the earliest "official" SARS-CoV-2 case with onset of Dec 10, 2019 was not related to the Huanan Seafood Market.

@Engineer2The - TheEngineer2 🇨🇦

8/ Sorting out some errors and misconceptions in the published cases

@Engineer2The - TheEngineer2 🇨🇦

1/ 🧵on the earliest (official) reported case of SARS2. It will take some explanation and the results are not what you'd expect. Follow the trail of crumbs. 🧐

@Engineer2The - TheEngineer2 🇨🇦

9/ Background on 51M the first Beijing case who was at a pharmacy near the market and onset of Dec 17, 2019

@Engineer2The - TheEngineer2 🇨🇦

One of the new patients disclosed in this paper is 51M. He was previously not known to be an early patient and/or connected to the Huanan seafood market. However, he is among the earliest genome patients in December 2019 and connected to the market.

@Engineer2The - TheEngineer2 🇨🇦

10/ Sorting out and resolving some errors in various published papers

@Engineer2The - TheEngineer2 🇨🇦

1/🧵on some errors I've seen in various covid-19 papers. I'll add to this as time goes on, as there are many problems to cover. If I get any wrong feel free to debate.

@Engineer2The - TheEngineer2 🇨🇦

11/ Information on the Shi Zhengli Chinese bioengineering research grant

@TheSeeker268 - The Seeker

The project summary of Shi Zhengli's 2018 NSFC project. Via @Engineer2The "We will target the SL-CoV strains which use ACE2 as receptor and investigate the binding ability between the S-RBD and ACE2 of different origins.. web.archive.org/web/2021081720…

@Engineer2The - TheEngineer2 🇨🇦

12/ Showing how the market case map closely reflects transit rail stops

@Engineer2The - TheEngineer2 🇨🇦

1/ I ran this crude comparison of the Wuhan rail stops vs the Weibo case data for Jan-Feb 2020. I certainly don't claim to be a mapping expert, but thought it's easy to see a a pattern in the data.

@Engineer2The - TheEngineer2 🇨🇦

13/ Some more info on early cases

@babarlelephant - babar

First preprint with @Engineer2The @Drinkwater5Reed @Franciscodeasis on our dive into early Covid cases, focusing on sequences. https://zenodo.org/record/6672912 We found some raw reads unpublished as sequences (https://academic.oup.com/cid/article/71/15/713/5780800 ) and that Beijing's 1st case was earlier than thought

On some unpublished early SARS-CoV-2 sequences The origin of SARS-CoV-2 is still unknown: the chain of events that brought a virus whose close relatives are found in Rhinolophus bats in the Yunnan province and in Laos to the Huanan seafood market in Wuhan in early December 2019 remains to be elucidated. In particular, the non-market patients and the genetically more ancestral Lineage A remain mysterious. A retrospective analysis identified 174 patients with onset in December, among them only 15 have been sequenced and published, often multiple times. By collating as much data as possible on early cases we found some data on 65 patients with onset in December 2019. Furthermore, we detected two patients who had been sequenced, but whose sequences were never uploaded to a public database and whose raw reads, although published, were not reanalyzed. We also present some information on the first Beijing patient, who had an onset date of December 17, 2019 and was related to the Huanan market outbreak. Using the collated information, significant progress has been made towards solving the discrepancies in the early sequences. A phylogeny of 19 early patients is presented, based on onset dates, as well as several tMRCA estimates – falling in late November. zenodo.org

@Engineer2The - TheEngineer2 🇨🇦

14/ A closer examination of which market case first infected the market. https://t.co/GMdIZv5jZM

@Engineer2The - TheEngineer2 🇨🇦

1/ 🧵exploring which human case could have been the first market case by examining details of each case. https://t.co/fs6hFfMR6O

@Engineer2The - TheEngineer2 🇨🇦

15/ Some details of the Ben Hu research grant. https://t.co/44D09VuFfj

@Engineer2The - TheEngineer2 🇨🇦

1/ 🧵on the WIV research grants and researcher Ben Hu. Ever since the discovery of the DARPA proposal, some key questions remain unanswered. https://t.co/wVPNadJCSl

@Engineer2The - TheEngineer2 🇨🇦

16/ Some details of the Ge Xingyi research grant. https://t.co/kJpCsiE4Z7

@Engineer2The - TheEngineer2 🇨🇦

1/ In a previous 🧵, I covered some aspects of the Ben Hu (WIV) research grant. That was just the introduction. 😉 Next let's talk about the Ge Xingyi grant. https://t.co/St8MnzChkK

@Engineer2The - TheEngineer2 🇨🇦

17/ Some details of the Zhao Xuesen research grant. https://t.co/LPbO5sJbpF

@Engineer2The - TheEngineer2 🇨🇦

1/ In previous 🧵, I covered the Ben Hu and Ge Xingyi research grants. Let's keep going on same theme of risk of interspecies transmission. https://t.co/U2xn4Gj4Ou

@Engineer2The - TheEngineer2 🇨🇦

18/ Thread dismissing the Huanan Seafood Market as the origin of the pandemic. https://t.co/j3bGovvwPq

@Engineer2The - TheEngineer2 🇨🇦

1⃣ The origins of SARS-CoV-2 remains unsolved. Whether zoonotic in origin, or the result of lab leak remains a mystery. Here I show why the Huanan Seafood market can be dismissed as the origin of the pandemic. https://t.co/8iRwTUZwfi

@Engineer2The - TheEngineer2 🇨🇦

19/ More up to date details and citations for 48F and 57F cases. https://t.co/CgY7oLWnLg

@Engineer2The - TheEngineer2 🇨🇦

1⃣ A review of two earliest "officially recognized" Covid-19 cases. Among the most confusing cases are 57F Huanan Seafood Market shrimp vendor and 48F1 XX Yin.

@Engineer2The - TheEngineer2 🇨🇦

20/ A partial list of WIV workers. https://t.co/N9h5SEwck2

@Engineer2The - TheEngineer2 🇨🇦

1/ 🧵I've been meaning to do for some time. We may need to go back to the future to see what's correct after new intelligence is released. There are 700+ WIV personnel. Only 20-30 work in the emerging infectious diseases group (specializing in SARS like virus). Who are they? https://t.co/4rlkTD2NAh

@Engineer2The - TheEngineer2 🇨🇦

21/ A discussion of the Lineage A family cluster and the onset dates. https://t.co/FkClHpqLvk

@Engineer2The - TheEngineer2 🇨🇦

1⃣ A review of 62M LineageA, onset Dec 1, 2019 and early cases in Huang et al 2020 Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China DOI 10.1016/S0140-6736(20)30183-5 https://t.co/CD22KNFO4D

@Engineer2The - TheEngineer2 🇨🇦

22/ The Huanan turtle vendors and relation to Liu Deyan clinic https://t.co/eaarXTyDkD

@Engineer2The - TheEngineer2 🇨🇦

1⃣ 🧵On a couple of Huanan turtle vendor Covid19 patients visiting Liu Deyan clinic in December 2019. Putting the pieces together. 🧩

reSee.it AI Summary
The evidence suggests deliberate release of the variant factory. Anticorrelation is not an artifact, found in every sample. Raccoon dogs are not naturally infected with SARS-CoV-2. Contamination by samplers and cross-contamination caused false positives. Infection within an animal farm would spread to more cities. Human cases shed virus in toilets. Contaminated samplers and lack of training led to contamination. No evidence of animals or vendors shedding the virus. WCDC staff likely contaminated wildlife stalls. Geographic bias and cover-up methods were evident. Community members tested negative despite high seroprevalence.

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

1/It is a variant factory. They were deploying them. See archive.md/dorVk it is proof for deliberate release. @Daoyu15 https://t.co/D6x9SKDUCB

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

2/ 1. The anticorrelation is not an artifact. It is in every sample even within that “stall” itself. 2: that “cart” was PCR negative and Homo Sapiens are found in the sample. 3: nobody have ever found a naturally infected raccoon dog anywhere in the world. @Daoyu15 https://t.co/SaWf4ZP19J

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 2/引用 ❶Execept that in this case the fingerprints were found to be mainly of the victim, which don’t even know how to pull the trigger. archive.md/yyX0Z archive.md/4rVph archive.md/iw1Pz archive.md/DChUL @Daoyu15 https://t.co/oDRXZSAL34

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 2/引用❷ Raccoon dogs and civet cats are the 2 species least susceptible to natural infections by SARS-CoV-2. And by actually correlating with the virus on the body of the gun, the suspect’s fingerprint was found only on the trigger and while overlapping with @Daoyu15 https://t.co/WReHRVECPh

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 2/引用❸ the victim’s, the victim’s fingerptints are found where it is not possible to fire the gun—all animals other than Homo Sapiens are found on entirely different locations in that “stall” than SARS-CoV-2. The correlation are all poor to negative. archive.md/EzTib

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 3/❶ 4: the primary confounder was that the “stall” was closest to the toilets, archive.md/T2u0S archive.md/LJzSO archive.md/4cCHG archive.md/FskYn Closest to where the samplers entered and exited, and all of the samples inside was located @Daoyu15 https://t.co/ED9LDueyGT

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 3/❷ right where sampler contamination is expected to happen. archive.md/csYBM archive.md/lI04H archive.md/0A24q archive.md/JSQvc @Daoyu15

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 4/❶ In fact every single Q* sample came from contamination caused by samplers and cross-contamination afterward. https://t.co/FLlRsmkvuF archive.md/vlAgp archive.md/0O2TN It is most likely to have sampler boots trampling the carts and kicking the @Daoyu15 https://t.co/XCtAoeBKzQ

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 4/❷ cages after trampling through the toilet area, archive.md/CTP3i archive.md/BWZJL archive.md/ETjzS archive.md/2PM9Y Suits rubbing against the entrance and then against the sample tube in an awkward sampling site, @Daoyu15

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 4/❸ and possessed specific characteristics of a contamination-induced false positivity (ORF1ab primer aligned over without PCR+, no positive results again despite repeated samplibg attempts). @Daoyu15

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 5/❶ 4: the primary confounder was that the “stall” was closest to the toilets, archive.md/T2u0S archive.md/LJzSO archive.md/4cCHG archive.md/FskYn Closest to where the samplers entered and exited, and all of the samples inside was located @Daoyu15 https://t.co/DbP1ELVZbG

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 5/❷ right where sampler contamination is expected to happen. archive.md/csYBM archive.md/lI04H archive.md/0A24q archive.md/JSQvc In fact every single Q* sample came from contamination caused by samplers and cross-contamination afterward, https://t.co/1YQFVYpVey

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 6/ NGS is known to be less sensitive and more prone to cross-contamination and process-linked contamination than PCR. @Daoyu15 https://t.co/NIGyxO9I1G

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 6引 archive.md/vlAgp archive.md/0O2TN It is most likely to have sampler boots trampling the carts and kicking the cages after trampling through the toilet area, archive.md/CTP3i archive.md/BWZJL archive.md/ETjzS archive.md/2PM9Y @Daoyu15 https://t.co/Vfc1h2KUtR

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 6/引用❷ Suits rubbing against the entrance and then against the sample tube in an awkward sampling site, and possessed specific characteristics of a contamination-induced false positivity (ORF1ab primer aligned over without PCR+, no positive results again @Daoyu15

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@Daoyu15 6/引用❸ despite repeated samplibg attempts). Again, NGS is known to be less sensitive and more prone to cross-contamination and process-linked contamination than PCR. @Daoyu15 https://t.co/ktt39ezXaa

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

7/❶ archive.md/yyX0Z archive.md/4rVph archive.md/iw1Pz archive.md/DChUL In fact, not only were there zero reports at all that raccoon dogs or any of the animal species found in W6-29-33 was infected in nature with SARS-CoV-2, @Daoyu15 https://t.co/f4CjBi0IW3

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@Daoyu15 7/❷ but freuling et al used the entirely wrong strain, and the origin for those raccoon dogs were actually traced to Shandong and Hebei for all HSM suppliers, @Daoyu15

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@Daoyu15 7/引用 @Daoyu15 https://t.co/l9Ky9tN7dJ https://t.co/sc922FGihb

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@Daoyu15 There were no evidence that either raccoon dogs were infected in the market or evidence that they can be infected at all in the world. None of them are. archive.md/csYBM archive.md/HlJ9o archive.md/2sAST 🧑‍🔬not🦝 archive.md/vlAgp archive.md/gvHfw https://t.co/KUdLl6JCxk

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@Daoyu15 8/❶ Even worse, any kind of infection within an animal farm in China would inevitably infect a lot more cities archive.md/8JhAb archive.md/svZqO than just Wuhan as all of them supply more than one customers. @Daoyu15 https://t.co/vYRihUg9WH

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@Daoyu15 8/❷ Exclusively Wuhan and not even other markets in Wuhan, is strictly impossible. @Daoyu15

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@Daoyu15 8/引用❶ Even worse, any kind of infection within an animal farm in China would inevitably infect a lot more cities than just Wuhan as all of them supply more than one customers. @Daoyu15 https://t.co/SdwYKA99eG

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 8/引用❷ Exclusively Wuhan and not even other markets in Wuhan, is strictly impossible. archive.md/8JhAb archive.md/svZqO @Daoyu15 https://t.co/QIYEk1U8O3

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 9/ The toilets right next to the stall is where all of the human cases in the market shed the majority of their virus in. The Wuhan CDC had an infected sampler. They put bleach into the toilets to attempt removing their traces archive.md/rSaO9 archive.md/ef2JW https://t.co/U7ML3taJsK

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 9/引 archive.md/CTP3i archive.md/ETjzS archive.md/BWZJL The rules: must be contacted by a sampler. 🥼👖👢=positive. archive.md/NeybM archive.md/2PM9Y archive.md/RirQ7 Must not be frequently handled by a vendor. 🥩🥬🍄🛁📻☎️📦🧺=negative. https://t.co/gyjD9MPcjq

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 10/❶prior to actually sample the area, only to have that infected sampler shedding one last time with only human as the mammalian sequence in the last samples. None of these have a lineage read, so the possibility that the same @Daoyu15 https://t.co/R2WBMubcUM

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 10/❷ infected sampler dropped his PPE in archive.md/2PM9Y archive.md/RirQ7 31/12/2019 and contaminated the wildlife stalls later, can not be ruled out. @Daoyu15

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@Daoyu15 10/引❶ The toilets right next to the stall is where all of the human cases in the market shed the majority of their virus in. The Wuhan CDC had an infected sampler. They put bleach into the toilets to attempt removing their traces archive.md/rSaO9 archive.md/ef2JW https://t.co/qPgX0Y1yVo

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 10/引用❷ prior to actually sample the area, only to have that infected sampler shedding one last time with only human as the mammalian sequence in the last samples. None of these have a lineage read, so the possibility that the same infected sampler @Daoyu15 https://t.co/fpnXNauhqm

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 10/引用❸ dropped his PPE in archive.md/RirQ7 archive.md/2PM9Y 31/12/2019 and contaminated the wildlife stalls later, can not be ruled out. @Daoyu15

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@Daoyu15 11/❶Even worse, you can’t even archive.md/k0GC9 archive.md/C5oal rule out the human cases because early cases have an ascertainment rate of below 25% even in the market itself because the majority of even naive cases were mild to asymptomatic. @Daoyu15 https://t.co/IqIzIioUGJ

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@Daoyu15 11/❷ No entering major hospital no ascertainment. @Daoyu15

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@Daoyu15 All of the positive salples in the market itself can be ultimately traced back a contaminated suit, boot and pant surfaces of the samplers—these are mostly volunteers that have experience in neither scene preservation or aseptic techniques, contaminating surfaces and sample tubes https://t.co/v2JXkMe4uv

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@Daoyu15 12/ @Daoyu15 https://t.co/uRGxdNS7RA https://t.co/kQ02fq48QP

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@Daoyu15 Because most of their samplers and cleanup workers are volunteers that have no training at all on scene preservation or aseptic techniques (other than simple beiefing) and have no capacity to take proper caution not to contaminate the objects themselves before samples are taken. https://t.co/1EQgUxR8Rm

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@Daoyu15 13/❶Even worse, you can’t even archive.md/k0GC9 archive.md/C5oal rule out the human cases because early cases have an ascertainment rate of below 25% even in the market itself because the majority of even naive cases were mild to asymptomatic. @Daoyu15 https://t.co/xOjWh46khk

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 13/❷ No entering major hospital no ascertainment. @Daoyu15

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 14/❶Both toilets and infected samplers lead to contamination, and samples above head level archive.md/NeybM archive.md/RirQ7 is neither likely to become contaminated in the first place nor they allow SARS-CoV-2 RNA to last. Bleaching the @Daoyu15 https://t.co/cFh4xnyaLG

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@Daoyu15 14/❷ main street archive.md/FskYn archive.md/JSQvc drains and archive.md/bzeP1 the toilets themselves immediately prior to their sampling is indicative of them finding this issue and covering them up. @Daoyu15

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@Daoyu15 15/❶There is no evidence that any animals or vendors shed the SARS-CoV-2 in the market. All that existed were WCDC cleanup workers and samplers spraying the focused stalls with lineage B and dropping in PPE with lineage A, @Daoyu15 https://t.co/uvV2WTlEXH

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@Daoyu15 15/❷ and further WCDC samplers with contaminated PPE trampling the surfaces. @Daoyu15

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@Daoyu15 16/Also as there are no lineage reads in the wildlife samples, it is not possible to rune out the same WCDC staff member both dropped A20 and contaminated the wildlife stalls as he entered and sampled in the market multiple times. @Daoyu15 https://t.co/7hMYCuKkST

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@Daoyu15 16/引用❶Both toilets and infected samplers lead to contamination, and samples above head level archive.md/NeybM archive.md/RirQ7 is neither likely to become contaminated in the first place nor they allow SARS-CoV-2 RNA to last. Bleaching the main @Daoyu15 https://t.co/xFosz87WiI

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@Daoyu15 16/引用❷ street archive.md/FskYn archive.md/JSQvc drains and archive.md/bzeP1 the toilets themselves immediately prior to their sampling is indicative of them finding this issue and covering them up. @Daoyu15

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@Daoyu15 17/The official geographic bias was literally written in the annex D5 of the WHO report. @Daoyu15 https://t.co/w4JviOACWr

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@Daoyu15 17/引用X文❶ Here is official geographic criteria that was literally written in the annex D5 of the WHO report. @Daoyu15 https://t.co/4FQ93Lc6nk

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@Daoyu15 17/引用X文❷ Is it possible that with a community seroprevalence well reaching above 5% at the last months of 2020, any 67 community members sampled in January 2021 can still test “all negative”? They hid pre-Huanan cases, and it wasn’t that good a cover-up method. @Daoyu15 https://t.co/uR2fcBDZmT

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@Daoyu15 @rattibha unroll

@zijizhanchu_5 - 🇺🇸找出病毒真相-5🇬🇧🇯🇵🇨🇦🇺🇦🇮🇱🇰🇷🇩🇪🇫🇷🇮🇹

@Daoyu15 @rattibha @rattibha unroll

Saved - October 4, 2023 at 9:44 PM
reSee.it AI Summary
The SARS outbreak in Hong Kong in 2003 involved unexplained respiratory illness leading to death. Diagnosis relied on exclusion. A local CDC was established for disease surveillance. Linking it to the 1997 H5N1 incident, active surveillance and poultry vaccination prevented further human H5N1 disease. The concept of "One Health" emphasizes applying similar measures to humans. Continued active surveillance and poultry vaccination were implemented. Economic analysis findings will be shared later.

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SARS. 2003. Hong Kong. 'second definition included a person with an unexplained acute respiratory illness resulting in death after 1 November 2002, but on whom no autopsy had been performed' '... SARS diagnoses were based on exclusion...' Neil Ferguson https://ncbi.nlm.nih.gov/pmc/articles/PMC1693389/pdf/15306395.pdf

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Never let a crisis go to waste, and all. Golly, was the diagnosis-of-exclusion, no-autopsy-death SARS pandemic not quite what it appears? https://www.nature.com/articles/s41599-022-01467-z

From SARS to COVID-19: the role of experience and experts in Hong Kong’s initial policy response to an emerging pandemic - Humanities and Social Sciences Communications As one of the most densely populated places in the world, Hong Kong fared relatively well in the first year of the COVID-19 pandemic, with a very low number of cases and fatalities per capita. This was mostly due to the Hong Kong government, healthcare workers, and the general public’s institutional and individual memory after they successfully overcame the deadly severe acute respiratory syndrome (SARS) epidemic in 2003. However, while Hong Kong was well accustomed to measures such as wearing masks and social distancing, the cooperation of the Hong Kong public to government restrictions was highly affected by its local political context, especially after widespread anti-government protests began mid-2019. This brought the public’s trust in government to an all-time low, creating a political ‘new normal’, which underpinned how COVID-19 policies would be proposed, accepted, and implemented, if at all. To understand how science advice was offered and how public health decisions were made, this research investigates the evolution of Hong Kong’s science advisory mechanisms for public health from before SARS, after SARS, and during COVID-19 in 2020, including the roles of key organisations and departments, the establishment of new centres and committees, and the creation of workgroups and expert advisory panels. This paper compares and analyses the reasons behind these differences in science advisory mechanisms between SARS and COVID-19. The findings from this research reinforce the unquestionable need for robust science advisory structures and knowledgeable scientific experts to solve health-related crises, though more research is required to understand the ways in which science advice influences both policy decisions and public acceptance of these policies. nature.com

@_Escapekey_ - _Esc

oh look, they setup a local CDC as well, complete with disease surveillance ffs. how obvious can this get? https://en.wikipedia.org/wiki/Centre_for_Health_Protection

Centre for Health Protection - Wikipedia en.wikipedia.org

@_Escapekey_ - _Esc

Right, let me just link this to the 1997 H5N1 Hong Kong incident. https://t.co/N0puU0tUiJ

@_Escapekey_ - _Esc

... it's just impeccable timing. And always those fake, economic arguments. Hong Kong. 1997. H5N1.

@_Escapekey_ - _Esc

'Enhanced biosecurity, active surveillance together with targeted and evidence-based interventions in the poultry production, and marketing system together with poultry vaccination has prevented further human H5N1 disease' ONE HEALTH Just imagine if they applied this to humans.

@_Escapekey_ - _Esc

... and what was the net result? ONE HEALTH Continued, active surveillance. Poultry vaccination. Just imagine. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120750/pdf/978-3-642-36889-9_Chapter_254.pdf

@_Escapekey_ - _Esc

... and for the record, I found that 'economic analysis'. Will be back later.

Saved - May 10, 2024 at 4:27 AM
reSee.it AI Summary
CAS "pathogen host adaptation and immune intervention" is a major grant that continues DEFUSE. Politically significant data from China cannot be trusted due to tampering. The CCP and WEF are responsible for the intentional release of SARS-CoV-2. Early bat and pangolin datasets have been changed multiple times. All zoonosis datasets in China have been tampered with. Real forensic evidence from China has been tampered with. Chen's inclusion in early cases led to tampering of official data. China systematically moved cases from the lab to the market. Unlinked cases were closer to the market than linked cases, indicating ascertainment bias.

@NestCommander - Kevin W. McCairn PhD

https://x.com/daoyu15/status/daoyu15/status/1731414539324018732 CAS “pathogen host adaptation and immune intervention” is one of such major grants that they continued DEFUSE on. https://x.com/daoyu15/status/daoyu15/status/1747929537848266981 https://x.com/daoyu15/status/1748424486729253321

@Rebecca21951651 - Rebecca

DEFUSE going unfunded from DARPA will not stop @peterdaszak at @EcoHealthNYC @COVIDSelect @RandPaul

@NestCommander - Kevin W. McCairn PhD

Politically significant data are state secrets in China. There is no probability that they would remain untampered when released by China. web.archive.org/web/2023110113… archive.md/UODyy archive.md/kJDII archive.md/g2L31 archive.md/Z72Mb Lies and cover-up of China proves intent of https://web.archive.org/web/20231024174245/https://en.rattibha.com/thread/1714851707719668064 creating and releasing SARS-CoV-2. https://web.archive.org/web/20231029094650/https://en.rattibha.com/thread/1717097760942354718 A20, B5, Q61, inconsistency and inconsistency in the China “Huanan market” data and anomalies in data release times indicate significant tampering of these politically significant “data”archive.md/luOy6 archive.md/ryr5p https://resee.it/tweet/1714851707719668064 https://archive.md/2mQwP It is authoritarian takeover. The CCP and the WEF are both responsible for releasing and then covering up the origin for SARS-CoV-2. All of the “Huanan market” data cases or environmental samples have been tampered with. https://archive.md/VYCRK https://web.archive.org/web/20231024174245/https://en.rattibha.com/thread/1714851707719668064 Catastrophic data inconsistency and anomalies in outbreak response by China clearly indicate intent in deliberate creation and intentional release of SARS-CoV-2. https://archive.md/79xvI None of their “data” are trustable in any way. There in fact is an intent to fabricate data. https://archive.md/bUVYp https://archive.md/26Y3G The inconsistencies upon inconsistencies and problems upon problems of Chinese “data”. Tamper with data, and you get caught. And all of the “data” you post loses all credibility. In fact, all pro-zoonosis datasets from China have been tampered with. The key problems here is simply that absence proper versioning or custody of “data” they put up, on GISAID or in the WHO report, archive.md/0aHWr https://archive.md/Myt4u there is no credibility at all in any piece of “data” China made. @DrLiMengYAN1 archive.md/52DyQ archive.md/B0xlW https://web.archive.org/web/20231101133202/https://en.rattibha.com/thread/1718570491534061745 archive.md/kJDII archive.md/UODyy archive.md/g2L31 archive.md/Z72Mb archive.md/AjLkp archive.md/luOy6 archive.md/ryr5p A20, B5, Q61, inconsistency and inconsistency in the China “Huanan market” data and anomalies in data release times indicate significant tampering of these politically significant “data”.

reSee.it #30369 - @zijizhanchu_5 China's tampering with data and cover-up of the SARS-CoV-2 bioweapon is evident. Contamination from samplers and toilets explains the positive correlation with humans. Animal species showed no correlation. The market samples were contaminated, while personal items of vendors remained negative. The proposed amendments to the International Health Regulations would expand WHO's power and create a global authoritarian regime. The WEF is also pushing for a totalitarian global government. This information needs to be known by more people. resee.it

@NestCommander - Kevin W. McCairn PhD

Nearly all of the early bat and pangolin datasets including the critical RaTG13 amplicon datasets have been changed multiple times before or just as they were being published, day after delay as they change them according to requirements as fast as possible. Trust China for ANY origin-relevant “data” now? @washburnealex @humblesci B5, A20, F13, F46, Q61, none of the politically significant “data” of China can be trusted in any way. And the most likely origin of the “positive wildlife stall samples” in the first place, is the WCDC and the AMMS in Wuhan planted them under the command of the bioweapons program—and did a poor job doing so. They even covered up the fact that humans can shed the virus and blocked it from the national IVDC and CCDC until the aforementioned institutions have to visit Wuhan hospitals themselves and find out that human to human transmission can in fact occur. Tamper with data, and you get caught. And all of the “data” you post loses all credibility. All pro-zoonosis “early sample datasets” in China have been tampered with. Bad badgers in Q61, Inverse correlation between human*SARS-CoV-2 and total 300nt+ mammalian contigs in the samples The “market samples” dataset is just as tampered with and with clear artifacts left behind, as all the “animal origin” datasets uploaded by China previously. A20, B5, Q61, every single inconsistency within the “market dataset” directly implicate tampering of and therefore non-validity of the “data”.

@NestCommander - Kevin W. McCairn PhD

Real forensic evidence never get an explanation of official release from China. All China show you as “forensic evidence” have already been tampered with. Not just Ascertainment bias. The “data” have been actively tampered with. Why bleach the toilets before sampling it? Not only there were no gloves at all in the A20 stall W7-15-17, but the sample is both inconsistent in viral reads between 2021/2023 and in host fractions (despite claimed to be multiplex PCR directly from the original sample which should not change host fractions) between the lineage reads-free “metagenomic” and the lineage A “viral amplicons” datasets. Like all 26/03/2023 deposition date “datasets”, they were tampered beyond credibility (3/4 of all samples with an 2021 viral count have been changed, the 1 left was kept without any distinction indicate likely used as standard and debunking the attempted explanation of resequencing (all 4 are stated to be “resequenced via multiplex PCR” together) by contradiction (an unchanged B5 requires an 2021 resequencing date, F13/F54/A20 moved to resemble B5 in ratios in 2023 clearly a move to cover-up https://archive.md/ANS4Q the prior artifacts that became known when they begun monitoring relevant online info in March 2023). Not only Jan 12 samples were affected—for all sampling dates mutual information with all species have been destroyed with the scrambling of the host reads upon the inclusion of the 26/03/2023 upload date datasets. It is quite evident that removing contigs does not eliminate Correlation between humans and the virus because reads are removed proportionally. And all that scrambling just removed mutual information to all species, in all sampling dates. And still can not establish significant mutual information to any “susceptible species”. All zoonosis datasets in China have been tampered with, again artifacts are seen. As found out by NCBI FOIA. https://t.co/WOsGj2kqFZ@washburnealex Both of the PCoV BioProjects with changed DBs change them so that they can remove human DNA from the samples. (One left VERO bits behind, one removed so many sequences that viral concentration in the “metagenomes” were 50x-70x higher than their virus culture.) No explicit Chinese DBs on origin can be trusted in any way. Unfortunately, they failed to consider that there are alternative assemblies and non-standard hypervariable regions in humans though. arxiv.org/abs/2108.08163 arxiv.org/abs/2207.03288 Tamper with data, and you get caught. And all of the “data” you post loses all credibility. x.com/drlimengyan1/s… As for the reason why they need to fabricate the bat and pangolin data as they do with (A20, F13, F46)/B5 and Q61? x.com/drlimengyan1/s… They release a fallback to animal whenever the lab is scrutinized beyond denial. Another issue here for GISAID, is always that archive.md/0aHWr https://archive.md/Myt4u there is no credibility at all due to absence of proper versioning or custody of “data” they put up. archive.md/52DyQ archive.md/B0xlW @DiLiMengYAN1

@NestCommander - Kevin W. McCairn PhD

Chen lived in Shidong. Even by the annexes indicating his history. The only thing they did is that they moved him to Jianghan close to the market on the WHO maps. Also, Chen is not the only person infected in Shidong/Jiangxia and central Wuchang. Most were censored and only one of the two ambulances arriving in 31/12/2019 have been registered as a dot—likely because the origin wasn’t inside the Shidong prefecture/BSL-4 surroundings, and likely only because of being a close contact relative of Chen (contacting an known case). Chen’s accidental inclusion in the WCH’s first report of early cases and its subsequent media coverage mean that China have no choice but to tamper with the official data in an attempt to move him—while the HPHICWM attempt to whistleblow the “cluster 1” cases in 26-27/12/2019 generated from the WCDC’s leak of their culture stock (intended for sample manipulation) was blocked by the Hubei CDC, until the report included market cases as well in 29/12/2019. To save face, the CCP leveraged the fact that the WCDC is right nextdoor to the market and forced official media to only say that the cases were “close to the Huanan market” but not allowing the proximity to the WCDC to be reported. https://gab.com/Flavinkins/posts/109256201942085712 All dots they moved this way (up to 1/3 of all cases) was sent to Jianghan, https://archive.md/p3K3Z https://www.researchgate.net/publication/370635299_Greater_than_the_Sum_of_its_Parts_-_Aggregated_Wuhan_COVID-19_case_data_points_to_the_wrong_side_of_the_Yangtze_River_-_Rixey_-_20230509 especially to the immediate surroundings of the market, to scapegoat it and end up causing the “unlinked cases” cluster to be closer to the market than the “linked cases” cluster, despite supposedly the linked cases should be the only source of initial human to human transmission seeding and therefore the unlinked cases should cluster near the linked cases and not the market itself. This kind of improbable-under-null-hypothesis behavior is all over Chinese “data”. And of course, Chen lived right next to the WIV BSL-4 in every dataset other than the WHO report maps—including interview datasets in the same report which where he frequents (RT-mart in Jiangxia) and which early report indicate he “lived in Wuchang” and the first hospital Jiangxia 1st Renmin hospital which he visited first and sick at the second day. Also, China WHO/WIV covered up their earlier cases intentionally—it is not plausible for 67 samples from humans taken in 01/2021 to test “all negative”. https://www.nytimes.com/2021/02/12/world/asia/china-world-health-organization-coronavirus.html They systematically moved more than 3000 cases from the lab to the market and gave “cases data” that they wanted to push for market as first outbreak site to distance from the labs. https://archive.md/rYvu3 https://archive.md/UFrSv https://archive.md/nevZy https://www.researchgate.net/publication/370635299_Greater_than_the_Sum_of_its_Parts_-_Aggregated_Wuhan_COVID-19_case_data_points_to_the_wrong_side_of_the_Yangtze_River_-_Rixey_-_20230509 Such an result of having unlinked cases closer to the market than linked cases is not expected even under the null hypothesis of market origin, which we should see unlinked cases secondary to and cluster around the linked cases, and not the market itself. https://www.researchgate.net/publication/370635299_Greater_than_the_Sum_of_its_Parts_-_Aggregated_Wuhan_COVID-19_case_data_points_to_the_wrong_side_of_the_Yangtze_River_-_Rixey_-_20230509 Not only there were an complete absence of verifiability in Chinese cases, there is direct non-circumstantial evidence that they moved up to 3000 cases from Wuchang to Huanan. In fact, it is totally not normal to have unlinked cases closer to the market than linked cases—the only way this can happen is with ascertainment bias. Only near the market gets ascertained if not directly linked to it. Base rate neglect. They did the exact same thing when claiming that all 67 “pre-Huanan checkable cases” were “serologically negative”. Again, the social media associated here say “before Jan 18, 2020”. Included all Dec cases. https://www.mdpi.com/2220-9964/9/6/402 https://ghrp.biomedcentral.com/articles/10.1186/s41256-021-00200-8 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149375/ 135/92 cases in early peer-reviewed papers that went missing in the WHO report.

Flavinkins on Gab: 'https://gab.com/Flavinkins/posts/1088805315972559…' Flavinkins on Gab: 'https://gab.com/Flavinkins/posts/108880531597255968 https://gab.com/Flavinkins/posts/109147956977669077 Also, remember accountant Chen? (Why his dot was moved to the WCH if he lives in Wuchang/Jiangxia?) it turned out that it was not only his dot that was not in the right place. Every dot within the 2km radius of the Wuchang railway station was moved or removed. This is within the area that is expected to have the infectious disease and respiratory cases ultimately serviced by the “中部战区总医院”. The hospital that sees large-scale respiratory case anomalies the first in Wuhan on official records, where the decision to “enter battle stations” on 01/01/2020 was made because of an “unexpected and fast-growing anomaly in the respiratory disease surveillance data” beginning at least as late as 31/12/2019. There were dots that were east of this area, and there were dots that were south of this area, in locations with lower population density compared to downtown Wuchang and further from the market. (2 ambulances from Jiangxia on 31/12/2019, but only 1 dot on the WHO map and he wasn’t accountant chen…… (likely ascertained by contacting a HSM case on public transport, “试行诊疗方案”) (accountant Chen got to the WCH in 27/12/2019)) Considering how cases that were admitted to the “中部战区总医院” weren’t directly reported except for WH01 in 14/01/2020, (Only 1 out of the 4 known sequenced cases here were directly reported. WH03 is reported after transfer to the Zhongnan hospital, one of the 2 initial market cases reported in the location. WH02 and WH04 were not in the NNDRS dataset and displayed as “unknown” in the WHO report) and how they then report seeing more fever cases in a single day than the entire CDC pre-04/01/2020 onset dataset (the point when they have to expand their fever clinics), it is quite likely that cases that initially broke out in Wuchang were muted by admission into a hospital that is placed under a command that doesn’t have to report on the NNDRS, and that any cases found in Downtown Wuchang had their “residential addresses” altered to place them as close to the Huanan market as possible and out of the Wuchang area. This would not be the first time when cases that came from an “inconvenient” location were hidden inside PLA-operated hospitals to prevent them from being counted. https://gab.com/Flavinkins/posts/109701931477090563 Why the WMHC rejected the WHO’s demand for line listings of the 174 “NNDRS cases” in annex E2? Also, one dot in Jiangxia is one of the two https://gab.com/Flavinkins/posts/109048819612838694 ambulances that were seen in 31/12/2019 from Jiangxia. Only one become a dot (central Jiangxia as opposed to the Shidong prefecture). It is possible that this is Chen’s relative that “visited a local market”, meaning that this is a case that is ascertained by contact with an early case, and saved from removal because of post-27/12/2019 onset. No dot at all is inside the borders of the WuChang district, even when dots begin showing up east of it in less populated places further from the market. This is clearly artefactual, indicating attempt at breaking up the cluster in Wuchang.' gab.com
ResearchGate - Temporarily Unavailable researchgate.net
On W.H.O. Trip, China Refused to Hand Over Important Data (Published 2021) The information could be key to determining how and when the outbreak started, and to learning how to prevent future pandemics. nytimes.com
ResearchGate - Temporarily Unavailable researchgate.net
ResearchGate - Temporarily Unavailable researchgate.net
Exploring Urban Spatial Features of COVID-19 Transmission in Wuhan Based on Social Media Data During the early stage of the COVID-19 outbreak in Wuhan, there was a short run of medical resources, and Sina Weibo, a social media platform in China, built a channel for novel coronavirus pneumonia patients to seek help. Based on the geo-tagging Sina Weibo data from February 3rd to 12th, 2020, this paper analyzes the spatiotemporal distribution of COVID-19 cases in the main urban area of Wuhan and explores the urban spatial features of COVID-19 transmission in Wuhan. The results show that the elderly population accounts for more than half of the total number of Weibo help seekers, and a close correlation between them has also been found in terms of spatial distribution features, which confirms that the elderly population is the group of high-risk and high-prevalence in the COVID-19 outbreak, needing more attention of public health and epidemic prevention policies. On the other hand, the early transmission of COVID-19 in Wuhan could be divide into three phrases: Scattered infection, community spread, and full-scale outbreak. This paper can help to understand the spatial transmission of COVID-19 in Wuhan, so as to propose an effective public health preventive strategy for urban space optimization. mdpi.com
The comparison of epidemiological characteristics between confirmed and clinically diagnosed cases with COVID-19 during the early epidemic in Wuhan, China - Global Health Research and Policy To put COVID-19 patients into hospital timely, the clinical diagnosis had been implemented in Wuhan in the early epidemic. Here we compared the epidemiological characteristics of laboratory-confirmed and clinically diagnosed cases with COVID-19 in Wuhan. Demographics, case severity and outcomes of 29,886 confirmed cases and 21,960 clinically diagnosed cases reported between December 2019 and February 24, 2020, were compared. The risk factors were estimated, and the effective reproduction number (Rt) of SARS-CoV-2 was also calculated. The age and occupation distribution of confirmed cases and clinically diagnosed cases were consistent, and their sex ratio were 1.0 and 0.9, respectively. The epidemic curve of clinical diagnosis cases was similar to that of confirmed cases, and the city centers had more cumulative cases and higher incidence density than suburbs in both of two groups. The proportion of severe and critical cases (21.5 % vs. 14.0 %, P < 0.0001) and case fatality rates (5.2 % vs. 1.2 %, P < 0.0001) of confirmed cases were all higher than those of clinically diagnosed cases. Risk factors for death we observed in both of two groups were older age, male, severe or critical cases. Rt showed the same trend in two groups, it dropped below 1.0 on February 6 among confirmed cases, and February 8 among clinically diagnosed cases. The demographic characteristics and spatiotemporal distributions of confirmed and clinically diagnosed cases are roughly similar, but the disease severity and clinical outcome of clinically diagnosed cases are better than those of confirmed cases. In cases when detection kits are insufficient during the early epidemic, the implementation of clinical diagnosis is necessary and effective. ghrp.biomedcentral.com
Association of Public Health Interventions With the Epidemiology of the COVID-19 Outbreak in Wuhan, China Was there an association of public health interventions with improved control of the COVID-19 outbreak in Wuhan, China?In this cohort study that included 32 583 patients with laboratory-confirmed COVID-19 in Wuhan from December 8, 2019, through ... ncbi.nlm.nih.gov
Saved - April 14, 2024 at 6:03 PM
reSee.it AI Summary
Former Acting Assistant Secretary of State Thomas DiNanno has recently made claims that the intelligence community suppressed evidence of a laboratory leak, sparking discussions around the inconsistencies and lies surrounding the origin of SARS-CoV-2. Various posts have delved into the correlation between positive samples and human presence, as well as the degradation of the virus in the market. Additionally, there are mentions of the virus's adaptation to VERO cells and HAE cultures, the absence of natural infections in susceptible animal species, and the presence of animal CoVs in the market. The importance of proximity to toilets in determining positive samples is emphasized, along with discussions on sampler contamination and cross-contamination. The credibility of the official narrative is questioned, with evidence suggesting a silent outbreak before reported cases. The relationship between reverse transcriptases and the virus's evolution is also explored. Further scrutiny is placed on the GISAID sequences, highlighting the need for 2 billion hosts and the use of multiplex PCR with potentially flawed primer batches. Wildlife farms are noted to harbor fewer hosts, leading to lineages disappearing due to exhausted primer batches. Real inserts outside of the SARS-CoV-2 genome are disputed, and O-linked glycans are said to be absent in the spike protein. Positive samples are primarily linked to toilets and feces due to contamination, while the reliability of PCR+/NGS- and PCR-/NGS+ results is questioned. Concerns are raised about the fraudulent nature of the market-centered Chinese early cases data, with suspicions that the outbreak was likely introduced from outside the market. The Wuhan Institute of Virology (WIV) is accused of lying and tampering with data, including spraying virus into wildlife stalls and manipulating case residence and animal sequences. The involvement of different research institutions and the manipulation of samples and data are also highlighted, leading to calls for further investigation to uncover the true origins of the virus.

@NestCommander - Kevin W. McCairn PhD

https://twitter.com/r_h_ebright/status/1729164212159824154?s=46&t=wRQSWp_1VffWmS2vKQwhSA… "Former Acting Assistant Secretary of State Thomas DiNanno tells [Sky News]…that when his team unearthed explosive evidence that pointed to a laboratory leak…, the intelligence community ran interference in support of a natural origin narrative." https://x.com/daoyu15/status/1729168018763292778?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729167969534742873?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729167355098648987?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729324420622320082?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729309996591337972?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729308817584984345?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729329128690880596?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729312142879494212?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729338088911245679?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729322432891408531?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729167881777373569?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729343927344660868?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729312566239953140?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729309508219158746?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729309400148619375?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729309125472116878?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729310412578095602?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729311057519509963?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729310187012653224?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729167940287975863?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729308993607356568?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729309904702435644?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729342979910054375?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729325988176335278?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729309214131286030?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729325988176335278?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729167003418845329?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729328417479545131?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729336382592835689?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729167446370877872?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729313672324059374?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729328928719016252?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729312282541478102?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729333420160151996?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729355939214688755?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729326324379234644?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729309633842745689?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729310879529984416?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729329357955768803?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729329164577333641?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729314296969232712?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729321751727985061?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729167079838998667?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729168160191066410?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729329425798549590?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729356004482327015?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729333148692214124?s=46&t=wRQSWp_1VffWmS2vKQwhSA… https://x.com/daoyu15/status/1729324235578077646?s=46&t=wRQSWp_1VffWmS2vKQwhSA…

@R_H_Ebright - Richard H. Ebright

"Former Acting Assistant Secretary of State Thomas DiNanno tells [Sky News]…that when his team unearthed explosive evidence that pointed to a laboratory leak…, the intelligence community ran interference in support of a natural origin narrative." https://www.skynews.com.au/world-news/us-intelligence-official-linked-to-who-was-critical-in-downplaying-covid-lab-leak-theory-during-joe-bidens-90day-probe-into-virus-origins/news-story/70cec8fe1513491a421d45b12b45a8e7

Sky News Australia Sky News Australia skynews.com.au

@NestCommander - Kevin W. McCairn PhD

Details on the DOE Z division. Also, debunking Angie Rasmussen and ilk there again. Also None of their “data” are credible in any way.

@NestCommander - Kevin W. McCairn PhD

And no. DEFUSE does in fact propose to insert FCSes (human-specific cleavage sites in human proteins) into SARSr-CoVs. Especially when there is a mismatch to either the S1-S2 or the S2’ cleavage site, both in S2. (Such as when QTQTNSRS show up in an study-relevant Asian Sarbecovirus in 2018, where no isolated or even studied ones have an non-HT(V/A)S(L/I)LRS sequence.) If there is an infected animal, more than one spillover would happen, especially Guangdong. Why it is VERO E6 that was Wuhan growing best inside? Why all later lineages grow less effectively in it? Just fuse in a cell line that have a correct serine protease pathway. CaLu-3 also uniquely have no growth advantage to any known VOCs compared to Wuhan. Include in passage, and PRRA is more stabilized than it can ever be mutated or deleted, Proline included. arxiv.org/abs/2104.01533 An infectious clone is designed to be rescued. archive.ph/EiCQW Well, MN611520–definitely not a bat CoV. And of course, motivated reasoning like markolin can not explain how the “perfectly natural and consistent with bat sex” CoVs MN611520 and HKU4-HZAU-1 ended up one in a location without a Merbecovirus natural host (cotton but not bats or camels) and another inside an infectious clone backbone. And of course, WIV1, WIV16, Rs4874 and RsSHC014 count up to 4 published live isolates not “only 3” claimed by Shi. That is published isolates only. zenodo.org/records/570270… RaTG13 don’t grow outside immortalized kidney cells. These are just too many inconsistencies and obvious lies regarding the number of WIV Or EHA viral sequences AND isolates in their public claims. Then, there is an attrition problem where the idea that “the FCS worked impossibly well than design can anticipate” was really based on observed functions that have no bearing to pandemic potential and only recently attributed to the “specific context of the FCS”, in reality they just nee to put ENaC FCS into a QTQTNS massively mismatched S, then grow it once in VERO cells. All changes to a sequence will have half advantage and half disadvantage in the organism, but here the specific advantage of P681 “specific sequence of the FCS” is only in VERO cells, and the disadvantage however given that all natural isolate Bat Sarbecoviruses are 614D, is the complete destruction of all animal reservoirs as the incredibly unstable D614+FCS Spike got torn apart by the antibodies that would form in the animal before the FCS can emerge. The reason why no FCS exist in wild Sarbecoviruses.

@Dissenting2020 - Dissenting Skeptic

1\ I have seen a lot of really bad attempts at dismissing the Defuse proposal, with BS objections claims like: * It was all pseudovirus, no infectious virus was used or * they never said they planned to insert furin cleavage sites

@NestCommander - Kevin W. McCairn PhD

01, 03 and 04, 06 are disproven by the presence of severe and https://ayjchan.medium.com/evidence-for-a-natural-origin-of-covid-19-no-longer-dispositive-after-scientific-peer-review-af95b52499e1 https://zenodo.org/record/7169296 conclusion-disproving ascertainment bias within the CCP data. As well as cherry-picking of early genome data. https://www.researchgate.net/publication/362806429_Unwarranted_exclusion_of_intermediate_lineage_AB_SARS-CoV-2_genomes_is_inconsistent_with_the_two_spillover_hypothesis_of_the_origin_of_COVID-19 https://gab.com/Flavinkins/posts/109152915624650793 08 is disproven by the fact that there were no by-month data for Xiao Xiao et al, and there being no evidence of December 2019 animal sales that can be proven through an image that contained either features or metadata that permit them to be dated. 05 is simply wrong. Yunnan is the hotspot, Guangdong is linked to wildlife trade. Wuhan is neither. 09 is disproven as the specific pattern of RE sites are not directly linked to spillover probability and the probability that it is easy to clone with the standard BsaI/BsmBI through natural recombination is less than 1/32. https://gab.com/Flavinkins/posts/109255356915252021 03 is disproven since despite all the efforts, FCS continue to elude all efforts to find it. https://archive.ph/k7S6T https://archive.ph/Ga1iI https://archive.ph/vUy8n 07 is disproven by Marburg virus and also RSV. Especially RSV, which originated in polio vaccine research. https://gab.com/Flavinkins/posts/108661483685033341 https://www.bmj.com/content/344/bmj.e2398/rr/599724 As for 10, the actual graph seen—show humans and largemouse bass not raccoon dogs. Humans and several livestock species in the “志翔冻品商行” near the toilets are the only species with any mutual information at all, highly consistent with boot and suit contamination. All metric positive correlation is observed only in humans and the entirely non-susceptible livestock species sold near the toilets. Worobey failed to address the fact that “the neighborhood of Huanan market” was used during the early case collection process—he opted to remove directly linked cases but none of the critical annex D5 cases that were collected from “the neighborhood of Huanan market”. Exactly 32 of these dots exists, = 59-27. https://gab.com/Flavinkins/posts/109228312723838390 https://gab.com/Flavinkins/posts/109443089504009640 https://gab.com/Flavinkins/posts/109148677382700486 https://gab.com/Flavinkins/posts/108825678909519515 These were cases that were collected because of their geographical proximity—collect if lived in the neighborhood, regardless of hospital. Collect from other hospitals only if exposed to market. Collect from hospitals near the market. https://gab.com/Flavinkins/posts/109386394452941367 https://gab.com/Flavinkins/posts/108830214433800007 https://gab.com/Flavinkins/posts/109169722840473497

Evidence for a natural origin of Covid-19 no longer dispositive after scientific peer review Declaration of competing interests: The author of this article has co-authored a book, VIRAL: The Search for the Origin of Covid-19, with science writer Matt Ridley. The updated paperback of VIRAL… ayjchan.medium.com
Zoonosis at the Huanan Seafood Market: A Critique Here we review data supporting a zoonosis hypothesis at the Huanan Seafood Market (HSM). We undertake statistical analysis of case locations and wildlife stall locations. We additionally analyze environmental sampling and review the likelyhood of susceptible animals being present in Wuhan, and only Wuhan of all locations in China. We find insufficient data to support a zoonosis hypothesis and instead conclude that the most likely scenario is that an infected person brought SARS-CoV-2 to the HSM, sparking a superspreader event. zenodo.org
ResearchGate - Temporarily Unavailable researchgate.net
Flavinkins on Gab: 'https://zenodo.org/record/7005332 https://gab.com…' Flavinkins on Gab: 'https://zenodo.org/record/7005332 https://gab.com/Flavinkins/posts/108825099943844332 https://gab.com/Flavinkins/posts/108725195810150843 How could you claim “two spillovers” for SARS-CoV-2 if https://archive.ph/JVFuc positions 8782 and 28144 were found to be so unstable that they were observed to mutate within the same human host? And how could “multiple polytomies” indicate “double zoonosis” when it literally appear in every superspreading cluster and major variant of SARS-CoV-2 today? https://gab.com/Flavinkins/posts/109016556387587166 https://gab.com/Flavinkins/posts/109179292343046033 Your simulation is wrong if what you claim to be extremely unlikely, happens all the time. https://gab.com/Flavinkins/posts/108956120173554636 https://gab.com/Flavinkins/posts/108974982120327267 https://gab.com/Flavinkins/posts/108983088984087090 https://gab.com/Flavinkins/posts/108941081553382213 It just happened that the phylogenetic tree topology of SARS-CoV-2 once you begin to look past 14/02/2020, resemble perfectly that of measles. Another virus with superspreading, one that appears only in humans. https://gab.com/Flavinkins/posts/109025416744564920 https://gab.com/Flavinkins/posts/108860074766577121 https://gab.com/Flavinkins/posts/108922841820898119 https://gab.com/Flavinkins/posts/108763931290402313 Here is why you get the polytomy at lineage B. (28144T and 8782C confers greater disease severity and higher transmissibility, which is also why lineage A went extinct) https://gab.com/Flavinkins/posts/108996982333862686 https://gab.com/Flavinkins/posts/108869387343860968 (Also hint: http://nature.com/articles/s41467-021-26884-7 https://archive.ph/ejdiJ one of the earliest superspreading events of lineage B after the Huanan seafood market superspreading event originated from an infected brain—such compartments accelerate viral evolution and skews the lineage B MRCA backward as the result). Also, quasispecies should not be ignored when it comes to the modeling of phylogenetics and phylodynamics of RNA viruses. https://gab.com/Flavinkins/posts/108861410320430025 https://gab.com/Flavinkins/posts/108864606801240300' gab.com
Flavinkins on Gab: 'When a synthetic recombinant genome for a coronav…' Flavinkins on Gab: 'When a synthetic recombinant genome for a coronavirus is constructed, fragments are selected from relevant natural bat isolates with a requirement that the type IIS restriction pattern using the planned enzymes on the resulting assembly should enable easy cloning and efficient manipulation—a less than 1 in 100 chance for this to happen randomly by chance for recombination outside the S1 region of the genome, and completely irrelevant to spillover. When a natural virus spills over, there is very little effect (within 1 order of magnitude) on the chance that some specific strain would end up becoming the pandemic strain for recombination ancestry outside the S1 region. There is no requirement that a strain that spills over must be a strain that is easy to clone by the 2 most popular type IIS restriction enzymes that were used in CoV genome assemblies, and the chance given natural spillover of an ancestry that had an efficient type IIS RGS system without modification is the same chance as finding one such strain in nature just by 1 single random sampling—so far no specimen from Asia satisfy this on their individual genomes. Again, which sequence on the ReCCA graph were not sampled from nature? Unfortunately, the so-called “natural recombination ancestry” argument may well just be one of the many ways workable coronavirus genomes are “recovered” from a set of otherwise unisolated samples. Whatever you reconstruct out of natural isolates for a clone, it must be easy to clone. It can be from one of the rare samples you find with an easy-to-clone pattern, or it could be one of the combinations of various contigs from sequencing a pooled sample. It could also be a chimeric genome constructed using fragments selected from related wild isolates with a requirement that the result is easy to clone. When a strain spills over naturally, there is no requirement that it must be easy to clone—restriction enzymes work on DNA not on RNA, and there is no reason why the specific combination with an easy to clone site pattern must be selected other than the posterior claim “it happened” (ReCCA construction used SARS-CoV-2 genome as reference). This is a circular argument as the claim that “the SARS-CoV-2 genome with its unusual combination of type IIS sites is the result of a natural spillover” assumed P(spillover|strain have good site combination)>=0.5 while P(strain have good site combination)<<0.5 with the only justification “we observe that the SARS-CoV-2 genome is easy to clone and can be constructed using a combination of fragments from some 8+ different “bat virus strains”” only able to justify this implied probability assumption with the assumption “SARS-CoV-2 is the result of a natural spillover”, a hypothesis that is being tested in the type IIS RE site analysis paper in stead of an underlying assumption. In conclusion, while a ReCCA with an easy to clone type IIS site combination with the go-to enzymes used for assemblies of this length is a possible combination of the bat coronavirus sequences known from sampling, there is no justification for this hypothetical and still unsampled ReCCA to be the only possible combination where a spillover is possible or that a spillover strain will have a probability that it will be easy to clone being >0.5 while the chance of finding such a strain from a random sampling from the wild being only about ~0.01.' gab.com
Flavinkins on Gab: 'archive from @daoyu15 about the FCS, Marburg viru…' Flavinkins on Gab: 'archive from @daoyu15 about the FCS, Marburg virus and the initial case density in Wuhan (once the restriction on diagnosis toward Huanan market contacts were lifted; from Weibo case alarms.) https://archive.ph/12GHD' gab.com
Re: Polio eradication: a complex end game bmj.com
Flavinkins on Gab: 'https://archive.ph/W2Rjj https://archive.ph/87AzE…' Flavinkins on Gab: 'https://archive.ph/W2Rjj https://archive.ph/87AzE https://archive.ph/dmOXT “ As @Daoyu15 pointed out, one reason for the clustering of early cases around the Huanan wet market without a direct link to it was that authorities were actively trying to identify cases in that area.” (Cases are collected if they have worked at or visited the Huanan market or lived nearby regardless of which hospital they were admitted to, or if they were admitted to one of the “several hospitals (near Huanan market)”) @Tony_vanDongen https://gab.com/Flavinkins/posts/109169722840473497 "the neighborhood of Huanan market" was used as a criterion for "continued epidemiological surveillance" happening all the way until ~04-05/01/2020. https://gab.com/Flavinkins/posts/108724146438967181 https://gab.com/Flavinkins/posts/108825678909519515 And the “initial cases” are also ascertained only through a historical bias which is that the Wuhan municipal health committee and CDC looked only at the Huanan market when monitoring EID cases. The “initial reports” were recognized only by the market cases within them. https://gab.com/Flavinkins/posts/108939607348559500 https://gab.com/Flavinkins/posts/109400102483795216 As for what happened “after 03/01/2020”? 1: compiling case reports and validating them (at the JinYinTan hospital) takes time. https://gab.com/Flavinkins/posts/108731797608502118 https://gab.com/Flavinkins/posts/109169722840473497 https://gab.com/Flavinkins/posts/109909275555101121 2: “试行诊疗方案” and “入排标准”. https://gab.com/Flavinkins/posts/108742419028347251 https://gab.com/Flavinkins/posts/109382280015707894 https://gab.com/Flavinkins/posts/108724146438967181' gab.com
Flavinkins on Gab: 'https://gab.com/Flavinkins/posts/1087397635596456…' Flavinkins on Gab: 'https://gab.com/Flavinkins/posts/108739763559645668 https://gab.com/Flavinkins/posts/108723816541534100 The Hankou station was the #3 highest location on social media check-in in Wuhan, making up 8% all such check-ins. It is within Worobey’s 1% KDE contour. As it also serves as the first location connecting the international and air traffic from the Tianhe international airport with the bus, metro traffic within Wuhan and the train traffic to other locations in Hubei (and to an extent other locations in China too, especially with busier times of air travel), it serves as the distribution point of all traffic not only through itself but also through the Tianhe international airport, centering the epidemiological contribution of the two facilities combined—with a combined social media check-in number of 37185 (it have to handle all the traffic from people who checks in at both the Hankou station itself and the Tianhe airport) and being a public transport hub that sees people from all across the line 2 of the Wuhan metro, it become the #1 highest contributor to “case spreading nearby” in any epidemic in Wuhan, and almost the guaranteed first superspreading location for those that started abroad, or starting anywhere on line 2. (You need To first go through line 2, before changing to line 7 or line 4 if you want to reach the Wuchang or Wuhan railway station, if you start from the Tianhe airport or the WIV (Wuchang headquarters)……) https://www.sundayguardianlive.com/news/probe-wuhan-metro-line-2-spread-pandemic Include the effect of annex D5, and you have the reason why the “market unlinked” case “epicenter” is closer to the market to the “market linked” case epicenter. Train stations are liminal spaces. When you ask a case/person as where he/she worked at or visited recently, specific public transportation stations (especially the metro station part of the Hankou railway station, the busiest metro station on line 2 with average daily throughput (in and out of the metro station) of 135000+ persons daily + 85000+ persons daily going through the rails in the train station) would hardly come up as significant or be reported from memory. The same effect also breaks up any clusters of infectious disease that spreads from them, as even very large superspreading clusters within such a station would be mostly among strangers (not connected either by work or by home) and they would show no epidemiological link to each other at hospital admission part from geographical proximity from each other on the order of 1-2km. Recognition of an outbreak especially given how EID surveillance operates in China and the fact that it happened during the flu season in Wuhan (too much background signal to distinguish EID without consulting epidemiology) would only happen once the infection have spread to the nearest (and the only) wet market (with a stable, mostly elder population in close contact at work that is conductive to both the efficient spread and recognition of an outbreak of SARS-CoV-2, especially without a specific test) that was put under EID surveillance in Wuhan. https://gab.com/Flavinkins/posts/108939607348559500 While it is statistically very likely that households near a major transport hub will be visited through that transport hub (creates an “unconnected case”, only ones that would show up on search would be the ones in the “neighborhood of Huanan market” or in the cluster that is likely to visit “several hospitals(near Huanan market)” if they weren’t indirectly connected to the market by contact with a market case), any specific person living or working in any specific landmark near them is still only going to visit his/her own or family household, which is most likely several kilometers away from the landmark and will not be significantly overpresented in the vicinity of the landmark. Consequently, the “epicenter” of market-linked cases is further away from that of market-unlinked cases. https://gab.com/Flavinkins/posts/108923557475080584 “While other biases are certainly at work in the contour maps, the absence of any mention of it while referencing the social media check-in data from Sina Visitor System amounts to a certain cherry-picking of the data to fit a certain narrative.”' gab.com
Flavinkins on Gab: 'https://gab.com/Flavinkins/posts/1087504705358358…' Flavinkins on Gab: 'https://gab.com/Flavinkins/posts/108750470535835849 https://archive.ph/XtLXy https://archive.ph/MCqMS https://archive.ph/dmOXT https://archive.ph/lOYDr https://archive.ph/bXHLT https://archive.ph/p3dbM https://archive.ph/t502Z https://archive.ph/5f0O3 https://archive.ph/VXtu9 https://web.archive.org/web/20220312073852/https://www.nzherald.co.nz/world/covid-19-coronavirus-wuhan-doctor-tells-what-it-was-really-like/6Q23B4ISGLPT7U7QC34G4VDAA4/ https://archive.ph/8thla https://archive.ph/9znGJ https://archive.ph/6LuXg You know your data have a serious problem of bias when the supposed primary sources of infection (cases that are directly linked to the Huanan market) ended up having a center of distribution further away (less centered around) (which also indicate that the radial isotropic spread condition is already broken, even for cases that were directly linked to the Huanan market) from the Huanan market under a KDE analysis compared to the supposed secondary infections (cases that are not directly linked to the Huanan market). https://gab.com/Flavinkins/posts/108713658029563999 This is very clearly the effect of a sampling effort that focused on “several hospitals (close to Huanan market), Huanan market and Neighborhood of Huanan market”.—cases were collected if they have been exposed to or lived near the market regardless of which hospital they were admitted to, and if they were admitted to one of the “several hospitals (close to Huanan market)”. (Annex D5) https://gab.com/Flavinkins/posts/108731797608502118 Add in cases that were hospitalized and reported from at or after 03/01/2020, under the “试行诊疗方案” and the “入排标准” which included cases that have contacted, visited, lived with, studied with, accompanied or visited the same hospital ward as a case that was directly linked to the Huanan market (cases that are indirectly linked to the Huanan market), and you end up with the entire WHO report “2019 cases” dataset. https://gab.com/Flavinkins/posts/108750470535835849 https://gab.com/Flavinkins/posts/108750094802767000 https://gab.com/Flavinkins/posts/108758479291053016 https://gab.com/Flavinkins/posts/108726114014659302 https://gab.com/Flavinkins/posts/108722307673771849' gab.com
Flavinkins on Gab: '“So there are documented issues with their statis…' Flavinkins on Gab: '“So there are documented issues with their statistical analysis, but I don't think you even need to go that deep. The diagnostic criteria for early cases sought a market link in the beginning (they assumed zoonosis), which left crippling ascertainment bias in base data.” https://gab.com/Flavinkins/posts/109382280015707894 https://gab.com/Flavinkins/posts/109222936375023885' gab.com
Flavinkins on Gab: 'https://archive.ph/dmOXT https://gab.com/Flavinki…' Flavinkins on Gab: 'https://archive.ph/dmOXT https://gab.com/Flavinkins/posts/108747087048451126 (You can find exactly 32 yellow spots “unlinked cases” in the 25% KDE contour of the WHO unlinked data……) https://gab.com/Flavinkins/posts/108731797608502118 (note: may contain cases admitted to the Houhu ward of the Wuhan central hospital even if them having a later date of hospitalization)' gab.com
Flavinkins on Gab: '“1\ Perfectly explained by a bias towards cases n…' Flavinkins on Gab: '“1\ Perfectly explained by a bias towards cases near HSM Cases are allowed frm further away if they have a link, explaining why linked cases are further away. How does one explain this without a ascertainment bias? 1st gen unlinked cases would have to be infected by linked cases 2\ Those districts comprise pretty much all the dense area of Wuhan, no? 3\ The authors elsewhere confounded diagnostic critera with suspicion criteria for the huang lancet papet. There is simply no reporting of the criteria for suspicion in the paper, but it presumably follows what was documented by the WHO report, which includes market proximity” https://gab.com/Flavinkins/posts/109228312723838390 https://gab.com/Flavinkins/posts/108726114014659302 @EmaNymton90 https://gab.com/Flavinkins/posts/108724146438967181 https://gab.com/Flavinkins/posts/108825678909519515 https://gab.com/Flavinkins/posts/108742419028347251 It should also be noticed that the JinYinTan hospital that was in the Lancet report did not admit the cases themselves—in stead suspected cases were first searched out from the “various healthcare institutions in Wuhan”(e.g. the Inpatient records of healthcare institutions), first for cases linked to the Huanan market city-wide beginning in 30/12/2019, then for cases that lived in the neighborhood of the Huanan market or have been admitted to a hospital near the Huanan market beginning in 31/12/2019. Suspected cases were first transferred to the JinYinTan hospital, before they were “diagnosed” according to the Lancet report criterion and then end up in the Lancet report. These 59 “transferred” (59 diagnosed/41 confirmed) cases are the 59/41 cases that were in the WMHC early reports. The rest of the cases would be from the backfilling period (e.g. hospitalized “hospital admission” at of after 03/01/2020, corresponding to the cases in the 174 cases dataset with a date of onset at or after 24/12/2019) of the standards between 03/01/2020 and 18/01/2020 and were ascertained almost exclusively by a direct or indirect connection to the Huanan market, according to the “试行诊疗方案” and “入排标准”. https://gab.com/Flavinkins/posts/108825678909519515 https://gab.com/Flavinkins/posts/108731797608502118 https://gab.com/Flavinkins/posts/108724146438967181 https://gab.com/Flavinkins/posts/108742419028347251 As for any cases outside that initial market-centered 174, they just refuses to include them into the WHO report and cover them up in stead, even if they were eventually found after 15/02/2020. https://gab.com/Flavinkins/posts/108876830805701129 https://gab.com/Flavinkins/posts/109058474962594334 https://gab.com/Flavinkins/posts/108826503882566575 Hint: 59-27=? Also, they did not perform any backfilling efforts after that. They even rolled back their own backfilling efforts in prior publications when arriving at the “174 cases”. https://gab.com/Flavinkins/posts/109296343974418128' gab.com

@NestCommander - Kevin W. McCairn PhD

https://gab.com/Flavinkins/posts/109205261283826972 ReCCA is tautological and fictitious. https://gab.com/Flavinkins/posts/109863181504837302 https://gab.com/Flavinkins/posts/109465063042828622 https://gab.com/Flavinkins/posts/109255356915252021 BtSY2 is sequenced in 2018. https://gab.com/Flavinkins/posts/109399710986742685 BANAL is in the hands of the DOD in 2017. https://gab.com/Flavinkins/posts/109340247585238829 https://gab.com/Flavinkins/posts/109800300869616862 The only thing in the market that meaningfully focus the abundance of SARS-CoV-2 is “closest to the toilets”. As all non-human “susceptible species” failed to correlate positively with SARS-CoV-2 consistently or with significant mutual information. With no actual evidence of natural infection in any of the so-called “susceptible species” at all. Contamination artifacts from samplers, and not actual animals, Or even vendors, created all of the “positive environmental samples” in the market. A fact which fraudulently bleaching the toilets before sampling can not hide, And which they failed to realize or create the required secondary spillover outbreaks in other locations Which all zoonoses have.

Flavinkins on Gab: 'https://gab.com/Flavinkins/posts/1089460581208001…' Flavinkins on Gab: 'https://gab.com/Flavinkins/posts/108946058120800199 How would you go onto constructing a synthetic consensus backbone that would be 1: guaranteed to be rescuable. 2: easy to manipulate as fragments. 3: easy to manipulate even after being ligated? You would need to choose fragments with type IIS restriction sites such that 1: each individual type IIS site must have a precedent in its presence of absence in the wild in at least 1 Sarbecovirus (distance doesn’t matter here. Can be as distant as BM48-31. Abundance matters statistically (rarer individual sites are less likely to show up at spillover) but does not matter if you are making a clone. As long as you find your desired presence/absence of a site at a specific location in even just one genome, you can use it and be confident that it won’t break your consensus genome. This is governed by both the availability of individual side precedence in sampled genomes and by the location of the site, whatever that doesn’t break the genome and is optimal for cloning gets chosen) to ensure that highly conserved RNA structural motifs aren’t disrupted. 2: there must be no BsaI sites between two BsmBI sites and no BsmBI sites between two BsaI sites to minimize the need of double digestion. 3: the number of overly small fragments should be minimized while no overly large fragments should be present at all. That is, the Standard Deviation (S.D.) Of the length of the fragments shouldn’t be too high. 4: the number of fragments should be kept to as low as possible with the restriction that you can easily manipulate each individual fragment within a M13/pUC vector backbone, as an excess number of fragments are more difficult to keep track of or manipulate. The type IIS restriction sites in the SARS-CoV-2 genome matches all the requirements above. Other genomes in its clade (or even any that is found in Asia)? Not that much. Unfortunately since clonability does not translate to spillover potential (without a lab), the fact that easily clonable Sarbecovirus genomes are rare in general also translates into the possibility that one that ended up spilling over just happen to be one that is easily clonable as SARS-CoV-2, being low. Since the fragments are what that is being chosen for the consensus genome, site selection influences ReCCA especially for the segments that were located near restriction sites—the segments surrounding them are what that were originally chosen in the consensus construct, where one of the requirements used is that the pattern of type IIS sites within the selected set of fragments should make the final genome assembled from them easy to clone. Finally, how could all the non-SARS-CoV-2 genomes you use in the ReCCA graph have a BsaI site in the first position of the F3 fragment (this site is highly conserved in Sarbecoviruses found in Asia) , but the final ReCCA, supposedly generated from related sequences sampled in the wild, not having that site? Either the ReCCA algorithm itself have considered these sites as part of the synonymous sites that was used to infer the “highly conserved segments”, and included SARS-CoV-2 itself into the analysis (making the algorithm tautological), or it is the result of the consensus-generation process where the choice of segments and sites to be used for the consensus included an requirement for the ease of cloning and manipulation for the final genome—something that would matter if you are synthesizing and rescuing it in the lab, not so much for anything that “spills over from the wild”. https://archive.ph/VypuD' gab.com
Flavinkins on Gab: 'More importantly, when considering the current RG…' Flavinkins on Gab: 'More importantly, when considering the current RGS systems, it turned out that none of the supposed ReCCA components are compatible with the cloning scheme that somehow were identical in their RE site order and REase use between U.S. and Chinese publications—the 2 BsaI sites within the ORF1a strongly interferes with the F3 fragment of the cloning scheme and when these sites are absent in a few select genomes, interfering sites pops up elsewhere in the genome that nevertheless still make the systems unworkable. The SARS-CoV-2 genome is the only one of which both the conventional and the no seems type IIS cloning methods would work with BsaI/BsmBI for the assembly of the genome—allowing both the easy assembly and ease for revision of the genome in all labs that were involved in the DEFUSE program.' gab.com
Flavinkins on Gab: 'In fact, how the ReCCA algorithm functions (it pi…' Flavinkins on Gab: 'In fact, how the ReCCA algorithm functions (it picks the closest sequence to the SARS-CoV-2 branch On “phylogenetic trees constructed on each (very short) segment of the genome”) mean that it is impossible to reconstruct an ReCCA without inputing the SARS-CoV-2 genome into the algorithm (as the reference genome to be aligned against) and thus biasing the result catastrophically—to the point that it is impossible to distinguish this process from what that would be used during the construction of an consensus genome for an infectious clone (fragments are picked with a requirement that the sites on the selected fragments lead to a genome that can be easily synthesized and constructed), and removes any statistical power of “ReCCA” to argue that the specific type IIS restriction pattern of SARS-CoV-2 to be the only possible combination of sites that is “evolutionarily likely”. It once again, failed to provide any biological reason why a specific, <1-in-100, easy-to-clone pattern being any more likely to end up being the spillover strain (that must not use the SARS-CoV-2 genome itself as the reference when such probability is assessed) compared to the >99-in-100 other possible combinations outside the S1 With the “ReCCA components” (where none were easy to clone by themselves) that are not easy to clone, in a non-circular manner. https://gab.com/Flavinkins/posts/109288626916761348' gab.com
Flavinkins on Gab: 'When a synthetic recombinant genome for a coronav…' Flavinkins on Gab: 'When a synthetic recombinant genome for a coronavirus is constructed, fragments are selected from relevant natural bat isolates with a requirement that the type IIS restriction pattern using the planned enzymes on the resulting assembly should enable easy cloning and efficient manipulation—a less than 1 in 100 chance for this to happen randomly by chance for recombination outside the S1 region of the genome, and completely irrelevant to spillover. When a natural virus spills over, there is very little effect (within 1 order of magnitude) on the chance that some specific strain would end up becoming the pandemic strain for recombination ancestry outside the S1 region. There is no requirement that a strain that spills over must be a strain that is easy to clone by the 2 most popular type IIS restriction enzymes that were used in CoV genome assemblies, and the chance given natural spillover of an ancestry that had an efficient type IIS RGS system without modification is the same chance as finding one such strain in nature just by 1 single random sampling—so far no specimen from Asia satisfy this on their individual genomes. Again, which sequence on the ReCCA graph were not sampled from nature? Unfortunately, the so-called “natural recombination ancestry” argument may well just be one of the many ways workable coronavirus genomes are “recovered” from a set of otherwise unisolated samples. Whatever you reconstruct out of natural isolates for a clone, it must be easy to clone. It can be from one of the rare samples you find with an easy-to-clone pattern, or it could be one of the combinations of various contigs from sequencing a pooled sample. It could also be a chimeric genome constructed using fragments selected from related wild isolates with a requirement that the result is easy to clone. When a strain spills over naturally, there is no requirement that it must be easy to clone—restriction enzymes work on DNA not on RNA, and there is no reason why the specific combination with an easy to clone site pattern must be selected other than the posterior claim “it happened” (ReCCA construction used SARS-CoV-2 genome as reference). This is a circular argument as the claim that “the SARS-CoV-2 genome with its unusual combination of type IIS sites is the result of a natural spillover” assumed P(spillover|strain have good site combination)>=0.5 while P(strain have good site combination)<<0.5 with the only justification “we observe that the SARS-CoV-2 genome is easy to clone and can be constructed using a combination of fragments from some 8+ different “bat virus strains”” only able to justify this implied probability assumption with the assumption “SARS-CoV-2 is the result of a natural spillover”, a hypothesis that is being tested in the type IIS RE site analysis paper in stead of an underlying assumption. In conclusion, while a ReCCA with an easy to clone type IIS site combination with the go-to enzymes used for assemblies of this length is a possible combination of the bat coronavirus sequences known from sampling, there is no justification for this hypothetical and still unsampled ReCCA to be the only possible combination where a spillover is possible or that a spillover strain will have a probability that it will be easy to clone being >0.5 while the chance of finding such a strain from a random sampling from the wild being only about ~0.01.' gab.com
Flavinkins on Gab: 'Additional sample formats, such as “bat coronavir…' Flavinkins on Gab: 'Additional sample formats, such as “bat coronavirus isolate NNNNNN” are found in 2015 samples, whereas the 2016 sequences (the last sequences to be deposited into GenBank from known bat coronavirus searching papers in China/CAS) contained within their authors “Edward C Holmes”. This may hint on the role of E.Holmes on the handling of bat Coronavirus sample sequencing for samples collected “between 2015-2019”. Sequences MH315932-MH315944 have formats “XxNNNNNN”, however these contained samples from as early as 2013, making it difficult to ascertain as where these samples came from. However, these numbers (and the “Spread and Geographic Structure of SARS-related Coronaviruses in Bats and the Origin of Human SARS Coronavirus” paper, with E.Holmes in the GenBank records) contained the only deposited CAS samples known to be collected in 2016, which could be taken as evidence of E.Holmes and WIV collaboration for sampling efforts and sequence/sample sharing, in the 2015-2019 period. A collaboration (sample/sequence sharing) between Guangdong and Wuhan institutions through E.Holmes https://zenodo.org/record/6849652#.Y38toiW8klT , can not be ruled out. The original GenBank deposition date of 2018 seems to coincide with the supposed sampling date of “BtSY2”. We now know that some of the samples taken in 2018 under E.Holmes contained RBD sequences related to the Omicron strain of SARS-CoV-2. The question becomes: Why you are taking samples from bats (dissected rectum), without performing some kind of tests immediately after sampling, as early as in 2015-2018, given that an ethics approval for the destructive sampling of wild animals in China required some kind of academic program to be first sent for approval, which have to include the detail of the exact experiments that requires the dissection of the bats and collection of the rectum samples. If any kind of testing/screening/experimentation were done initially on the samples (immediate experimental plan would have to be provided for the sampling proposal to be justified and approved—and “for storage, until some technology that doesn’t yet exist until late 2021” isn’t one of them), what were the results? Why, despite sampling of bats by EHA/CAS and expeditions into Yunnan/Mojiang mine continued all the way until 2019, the last such sequences deposited from China was sampled in 2016? If “This research, including the procedures and protocols of specimen collection and processing, 262 was reviewed and approved by the Medical Ethics Committee of the Yunnan Institute of 263 Endemic Diseases Control and Prevention. (No. 20160002).”, why weren’t the results immediately made available to the public as the samples were collected and processed over the course of 4 years? RNA samples are after all, very fragile and does not tolerate long-term storage very well. When were these actually sequenced? (I see 15 different sequencing machines in the FCIDs, with FCIDs ranging from H2 to H7, HG to HY, and sequencing on the same machines hundreds of runs apart. This could indicate sequencing done immediately after sampling) And if these were sequenced before the pandemic, who had access to the sequences? Were these really “newly discovered” viruses, or just sequences that were put into embargo in the “great silence” of CAS accession numbers between 2016-2019, only recently released? (Should these samples be sequenced pre-pandemic, near their collection date in 2018, this could be corresponding to phase 1(QS0) of DEFUSE. After sequences generated as early as in mid-2018, there would be more than 1 year to work with the cloning and culture experiments.) The machines found in https://github.com/Augustpan/Individual-Bat-Virome/blob/main/raw_data/lane_id_table.csv Are: A01426 A00821 A00920 A00270 A00877 A00289 A00881 A00783 A00253 A00917 A01045 A00808 A00459 A01415 A01050 The samples with BtSY2 are: S18CXBatR24 @A00917:648:H3Y25DSX2:4 S18CXBatR29 @A00783:739:H3V32DSX2:3 Both appeared to be on the earliest run on that particular machine, where the run contained no sample from 2019, and where the flow cell ID were from an early era (H3). Archive for read mappings: https://archive.ph/CuzzR Archive for lane IDs: https://archive.ph/IKxD1' gab.com
Flavinkins on Gab: 'To see more about the DOD-sponsored Institut Past…' Flavinkins on Gab: 'To see more about the DOD-sponsored Institut Pasteur sampling of the BANAL caves in 2017 and the censorship of even the already-sequenced batflies library https://gab.com/Flavinkins/posts/109150240613656613 https://gab.com/Flavinkins/posts/109139121799069783 https://gab.com/Flavinkins/posts/108782000872829271 http://gab.com/Flavinkins/posts/108745003276913992 https://gab.com/Flavinkins/posts/108938621623162894 https://gab.com/Flavinkins/posts/108961381086722860 https://gab.com/Flavinkins/posts/108808844066927838 https://gab.com/Flavinkins/posts/109198525541365424 https://gab.com/Flavinkins/posts/109222447665307488 PREDICT-2 and EHA activity in SE. Asia including Southern Laos https://gab.com/Flavinkins/posts/109079936391006382 https://gab.com/Flavinkins/posts/109079963106312117 Past lab escapes from IP france https://gab.com/Flavinkins/posts/108929427082821215 And on the type IIS REase found in IP cambodge https://gab.com/Flavinkins/posts/109215673255176764 https://gab.com/Flavinkins/posts/109216529429569399' gab.com
Flavinkins on Gab: '@Graviola_Finland @EIGARBARINO https://archive.m…' Flavinkins on Gab: '@Graviola_Finland @EIGARBARINO https://archive.md/8rlbT FULL TRANSLATED TEXT of the briefing of the Ministry of Defense of the Russian Federation on the analysis of documents related to military biological activities of the United States, made on January 30, 2023 with the supporting documentation that it provided. https://gab.com/Flavinkins/posts/108782801366501491 https://gab.com/Flavinkins/posts/108908816879287433 https://gab.com/Flavinkins/posts/108808844066927838 https://gab.com/Flavinkins/posts/108808523459345532 It confirms that sampling and researching effort for S.E. Asia is in deed being conducted in these labs. Anomalies regarding the Caspian sea in 2019: https://gab.com/Flavinkins/posts/108949034317465342 EHA activity in the Caspian sea region, up to 2019: https://gab.com/Flavinkins/posts/108782587895528182 https://gab.com/Flavinkins/posts/109139121799069783 https://gab.com/Flavinkins/posts/109529211058973737 DOD, IP laos and batflies🦇🪰: https://gab.com/Flavinkins/posts/108961381086722860 https://gab.com/Flavinkins/posts/108938621623162894 https://gab.com/Flavinkins/posts/108782000872829271 https://archive.md/JZkwi EHA, Laos and PREDICT-2: https://gab.com/Flavinkins/posts/109158749548555994 https://gab.com/Flavinkins/posts/109079936391006382 https://gab.com/Flavinkins/posts/109079963106312117 https://archive.md/hMp7x 🦇🧪🥣? https://gab.com/Flavinkins/posts/109150240613656613 https://gab.com/Flavinkins/posts/109198525541365424 https://gab.com/Flavinkins/posts/109222447665307488 https://gab.com/Flavinkins/posts/109728264957247673 https://archive.ph/3fFfn https://archive.md/T6sN5 (No wildlife trade route linking Wuhan to Laos…… But plenty of sampling route linking Laos to labs both in Wuhan and on the East Coast……)' gab.com

@NestCommander - Kevin W. McCairn PhD

@NestCommander - Kevin W. McCairn PhD

Except that 1: ZC45 and HKU3 are not SARS-CoV-1 or SARS-CoV-2. And 2: the raccoon dogs are locally wild-caught in Wuhan, tested and negative and 3: the animal-specific viruses are in perfect positive correlation with the animals, the SARS-CoV-2 is in consistent correlation with significant mutual information only with Homo Sapiens. And 4: the FCS itself optimized to cell cultures, HAE/VERO and CaLu-3, mutated in live hosts.

@NestCommander - Kevin W. McCairn PhD

Because Homo Sapiens is still the only species that they can get infected at all, if you zoom in and correlate between animals and viruses, You get animal-specific viruses being correlated strongly positively to the animals, and SARS-CoV-2 being positively correlated consistently or with significant mutual information only with Homo Sapiens.

@NestCommander - Kevin W. McCairn PhD

archive.md/yyX0Z archive.md/iw1Pz archive.md/4rVph archive.md/DChUL None of the “susceptible species” found in the market had a single report of natural infections by SARS-CoV-2, anywhere in the world. Closest and second closest stall to the toilets=👢👖🥼contamination have the highest frequency of happening among sampling. Neither civets nor raccoon dogs are susceptible at all to natural infections with SARS-CoV-2 with zero reported cases anywhere in the world. None of the “susceptible species” found in those “wildlife stalls” have been reported anywhere in the world neither China nor Europe Japan Vietnam of an natural infection by either SARS-CoV-2 or even a relative of it. Nor bamboo rat, hedgehogs or porcupines. No Guangdong spillovers directly rule out infected animals in the wildlife trade—specific supply from Yunnan to Wuhan can not support an economically viable farm because the extremely low consumption rates in Wuhan. archive.md/e3615 archive.md/vWjZl Homo Sapiens is the only species that remain consistent and significant positive correlationship within those “wildlife stalls”. https://archive.md/gvHfw https://archive.md/LJzSO https://archive.md/4cCHG https://archive.md/gkquN And the real determinant and the primary confounding factor for “which stall have the most positive samples out of all samples https://archive.md/JSQvc https://archive.md/csYBM Is “closest to the toilets”, also “closest to the entrance to the market” for the wildlife stalls. https://archive.md/vlAgp https://archive.md/mwT8i This is because all of the positive results from the “stalls” were caused by contamination caused by the samplers either smeared out from the toilets or brought in from the outside. This is also the reason why nearly all of them were found below step height, the height below which accidental trampling and kicking are highly likely. https://archive.md/FskYn https://archive.md/lI04H All that were left were either directly from the PPE of the samplers themselves https://archive.md/rj1pV https://archive.md/VNr75 Or are located right where contamination either on the surface (near walls or doors to be rubbed against, give PCR+) or on the sample tubes (awkward, crammed location making it difficult to not touch the lip of the sample tube onto sampler suits, give PCR-) are likely. These locations are also the locations that are most likely being sprayed (below step height) and the max normal operational height (below waist height for devices recorded on photo) by a sprayer or other dispensing device equipped by a “hazmat suited worker” that entered from the main entrance and was seen fiddling around with the environmental surfaces in 02/01/2020–without collecting a single environmental sample despite operating equipments in a way that look like virology work from afar. As for any direct vendor-handled surfaces, the RNAse 7 activity (skin defense ribonuclease, attack membrane-bound pathogens especially with an incomplete glycan shield) inside these samples are so strong that all SARS-CoV-2 RNA is completely destroyed within a single day between collection to sample processing. https://archive.md/2PM9Y https://archive.md/RirQ7 https://archive.md/NeybM https://archive.md/BWZJL https://archive.md/CTP3i https://archive.md/ETjzS The possibility of the same infected sampler that dropped the contaminated PPE in 31/12/2019 then contaminated all of the wildlife stall samples whenever he/she enters through the toilet area for that sample run, can not be discounted as none of these subsequent runs contained a lineage read.

@NestCommander - Kevin W. McCairn PhD

They are also compatible with a scenario that the same culture that was used in the initial planting work was eventually used to adulterate A20 when demanded by the CCP after learning about the lineage A issue. https://archive.md/LJzSO https://archive.md/4cCHG The first set of samples https://archive.md/gkquN contained human residues inside that became the only species with consistent positive correlation or one with significant mutual information with the SARS-CoV-2 reads in the market, then with the demand to blame snakes and to make sure humans don’t make its way into the samples, they stained the mid-phase samples with obvious amplicon artifacts and “tested” the rest with the highly cross-reactive PREDICT ORF1ab primers to manufacture “positivity” which none contained a real read of SARS-CoV-2. Finally The very last positive wildlife stall samples https://archive.md/13bdP, taken after the cleaning of the toilet area https://archive.md/rSaO9 , contained no mammalian species other than Homo Sapiens, which represents the “pure” form of the contamination within the wildlife stalls that is brought into the market by a sampler. They now use a mixture of animals to add to their to-be-tampered-with sample, derived from an assemblage of frozen products taken from the market so that they can say that the positive human/virus correlation, persisting even into these samples, are caused by “differential preservation”—Unfortunately the animal mix did not contain any mammals, meaning that they in stead become direct evidence that lab based cultures of SARS-CoV-2 (infections and culture supernatants give mainly the virions and a genomic profile for the viral RNA, culture lysates gives the intracellular transcriptomic profile for the viral RNA and host Mitochondrial DNA that is seen in these final “storehouse” samples.)

@NestCommander - Kevin W. McCairn PhD

https://web.archive.org/web/20231213033617/https://en.rattibha.com/thread/1714233859276149199 https://archive.md/73xfX Once inside “wildlife stall A”, The only correlation crashed to Homo Sapiens. Others crashed to zero one metric or another. https://archive.md/8nN3k Same as in the positive samples. Ask “which species shed the SARS-CoV-2 where it is found” yield “Homo sapiens”. The animals correlated with their native viruses, and not SARS-CoV-2. https://archive.md/BrQy7 https://archive.md/eoaMn

@NestCommander - Kevin W. McCairn PhD

The “all samples” merely asks “which species is sold most uniquely in the stall closest to the toilets” with much of the animals being on the ground, entirely wrong sections, https://archive.md/eoaMn and a heavily confounded distribution pattern centered around the toilets same as W4-26-28 in Jan 01. https://archive.md/FskYn https://archive.md/gvHfw Also, Raccoon dogs are in fact, never found infected in nature at all, neither China nor Europe. archive.md/g2L31 archive.md/OIHeo Sampler contamination and cross-contamination. archive.md/LJzSO archive.md/VNr75 Never an infected vendor or animal. In fact, the samples in the market follows the rule which a positive sample archive.md/CTP3i archive.md/ETjzS archive.md/BWZJL must be contacted by samplers.🥼👖👢=positive. archive.md/NeybM archive.md/2PM9Y archive.md/RirQ7 And not frequently handled by vendors.🥩🥬🍄🛁📻☎️📦🧺=negative. Another oddity, is that for the entirety of the market, none of the personal items of vendors and none of the frequently directly handled objects from boxes to baskets to cashiers, were positive. This is because SARS-CoV-2 RNA specifically is extremely unstable when on surfaces that are highly touched by a human—D614 especially because the Spike were too sparse to form a shield defending against invading RNAse 7. archive.md/LJzSO In fact, all of the positive samples in that “wildlife stall A” and the majority of the positive samples in the market is below step height—they are contaminated by the toilets and archive.md/4cCHG archive.md/FskYn is the reason why the stall with the most positive samples out of all samples is the stall closest to the toilets. In both Jan 01 and Jan 12. The superfluous nature of SARS-CoV-2 is therefore evident—not shielded inside solid tissues because all of it is shed by humans, and with an incomplete glycan shield, any that landed where human skin contact frequently (vendor items like cashiers, knives and chopping boards, baskets, boxes, water cup = least stable like bangladesh banknotes, not frequently touched locations like bases of machines or non-door-knob locations of cages, loading surfaces of grounds, scales or carts = more stable like sewage. Sampler PPE like gloves or shoe covers=most stable, and the main vectors in spread contamination to the objects and sample tubes.) got destroyed by RNAse 7. No positive above waist height, because these locations are where archive.md/FskYn samplers can exercise more care and it is less likely especially for untrained private contractors or volunteers briefed only once to avoid contaminating (enough room to avoid touching the surface or sample tubes, high enough so samplers can see to avoid rubbing with their suits. Below waist height on the other hand, not even professionals can avoid kicking or trampling due to lack of line of sight, and rubbing of the suits onto the surfaces are inevitable. Crammed locations where samplers need to support their own weight with their hands, breaks even the most professional of techniques and lead to high chance of sample tubes lips being contaminated.). 👢on 🛒🏔👣=PCR+/NGS+, 🥼on🧪=PCR-/NGS+ aligning over the primers and negative before and after. archive.md/tlfNr archive.md/GvRcD https://assets.researchsquare.com/files/rs-885194/v1/78e0e6ce-4a76-48de-9f5c-76bab452bbe6.pdf?c=1665607885

@NestCommander - Kevin W. McCairn PhD

The effect of spreading contamination out of the toilets and the main entrance of the market is evident not only in that both Jan 01 and Jan 12 have the stall with most positive samples out of all samples being the stall closest to the toilets, but also evident in the form of multiple samples from nearby stalls with only human DNA, no human cases and positive samples. They represent the purest form of sampler-linked contamination. https://archive.md/gvHfw The only thing governing the probability for positivity of the environmental samples is “closest to the toilets” and “closest to the main entrance of the market”. And bleaching it before sampling https://archive.md/rSaO9, just archive.md/13bdP lead to samples with only human DNA and no other mammals at all in the last samples hyper specifically taken from the “wildlife stalls”. All they managed to get in an effort to verify the ORF1ab primer-overlapping and Jan01-negative/PCR-negative contaminated sample tube are amplicon artifacts, false positives without reads, cultures of the virus from in-lab contamination and not a single positive of any kind on the site for Q37 any more.

@NestCommander - Kevin W. McCairn PhD

https://assets.researchsquare.com/files/rs-885194/v1/78e0e6ce-4a76-48de-9f5c-76bab452bbe6.pdf?c=1665607885 Unlike animals including livestock, humans are neither sold nor butchered at the market. Their CoVs degraded catastrophically after 01/01/2020 and completely after 12/01/2020 leaving only artifacts behind. archive.md/13bdP archive.md/FskYn archive.md/gvHfw archive.md/4cCHG Animals that are sold and butchered at the market have their CoVs remain stable and are the only CoVs left detectable in February 2020. Bane of the Zoonati: W4-26-28. Especially W4-28 which don’t have human cases inside. Only 1 out of the 5 samples have any wildlife reads at all. Closest to the toilets. Trample contamination clearly evident. Guess which species never have a single sample which it is not present? Homo Sapiens. In both Jan 01 and Jan 12 the stall with most positive samples out of all samples are the stall that is closest to the toilets in the market. Guess why. Guess why they never hint on Pearson correlation or the positive samples at all? The formulation of the problem “which species shed the SARS-CoV-2” gives an easy verification for species correlation by looking in samples where you actually see the SARS-CoV-2. This process also normalizes out any spatial confounding factors especially when confounded by pathological spatial distribution of the samples (all PCR+ samples in one stall closest to the toilets), where “which species is sold uniquely in a stall” guarantee by lottery fallacy a random species (each stall have one to two species that are uniquely sold inside and have low prevalence in the overall market), but actually entering the stall or the samples where you see the SARS-CoV-2 result in a collapse of correlation where no species except Homo Sapiens landed on the same sections of the ground as SARS-CoV-2. And of course, despite also being enteric like animal CoVs, SARS-CoV-2 failed to persist in the market, indicating it being not inside solid tissues like the animal CoVs, and therefore, not able to withstand either RNAse 7 degredation or cleaning agent application like animal CoVs could. Despite evident strong fecal and rectal shedding, persistence was not guaranteed for SARS-CoV-2 because it is not found in butchered animal tissue, which have safely and durably protected all animal viruses in the market all the way down to the end of February 2020. In addition to the observation where nearly all positive samples are found below step height and none of them being found above waist height, where incontrovertible proof of boot and suit contamination exist in the form of positive samples with neither human cases nor wildlife DNA, (which even followed the expected pattern as boots first bring the virus inside from the main entrance, then get additionally contaminated by wildlife DNA from the W6 junction) The stark contrast between highly persistent animal CoVs and SARS-CoV-2 which degraded to nothing but artifacts within mere 12 days, clearly indicate that the SARS-CoV-2 in the market is not shed by animals.

@NestCommander - Kevin W. McCairn PhD

Because SARS-CoV-2 is not found in any animals which would be butchered and their tissues and guts and noses split open and spilled onto the market surfaces, the human respiratory and enteric shedding that deposited the SARS-CoV-2 into the toilets archive.md/gvHfw and then smeared onto the market stalls, can not shield the virus from being destroyed either by RNAse 7 activty or cleaning of the market. archive.md/LJzSO archive.md/4cCHG Consequently, only SARS-CoV-2 degraded over time in the market, nearly completely degraded in Jan 12 and completely degraded afterward leaving nothing other than amplicon artifacts and obviously in-lab cell culture lysate adulteration with no susceptible animals inside and with the intracellular human mitochondrial and viral Transcriptomes still intact archive.md/13bdP (they decay to only MtDNA and viral gRNA within two days ex-vivo unless in an -80C lab freezer—environmental surfaces like the market or even significant storage time after leaving the growth environment in working conditions can definitively not provide such conditions e.g. all the samples before them). archive.md/YGDiK Animal CoVs on the orher hand, persisted, with reads consistent with real, artifact-free genomes, and with no significant concentration changes that can indixate degredation, in Jan 01, Jan 12, Jan 26-27 Drains (the only SARS-CoV-2 read at that point was found in a sewage well on the opposite end as the wildlife stall that is connected to the municipal sewage system but not the wildlife stalls) archive.md/8nN3k and all the samples afterward, including samples taken after 15/02/2020. No indication of significant degradation or quality change of animal viruses and animal CoVs are observed in any sampling date indicating that they last at least 3 months and most likely longer, unlike human-sourced SARS-CoV-2 that last no longer than 15 days without sterile conditions. And unfortunately bleaching the toilets https://archive.md/rSaO9 https://archive.md/nAqKp even amplifies this problem—wildlife CoVs persisted, and no SARS-CoV-2 except adulterated using an laboratory culture so fresh that even the human Mt transcriptome (don’t last in the environment for 2 days or longer) and SARS-CoV-2 intracellular transcriptome (don’t last in the environment for a week or longer) are left inside.

@NestCommander - Kevin W. McCairn PhD

Unfortunately, all that existed for Q61 and Q70 are the result of cross-contamination from Q64 and Q68/Q69, All of which are on the ground and archive.md/YGDiK are the result of either lower level boot and foot contamination x.com/daoyu15/status… x.com/daoyu15/status… Same as Q64/Q68/Q69 (stepped on>kicked for contamination). x.com/daoyu15/status… All that archive.md/73xfX archive.md/8nN3k archive.md/FskYn archive.md/gvHfw exist for Q37 is the contamination of a sample tube by the gloves and suits of the samplers. The swab is clean, PCR-. The tube lip is contaminated, NGS+ with alignment over the CCDC SARS-CoV-2 ORF1ab primer pair. archive.md/LJzSO archive.md/4cCHG PCR+/NGS+ mean the virus is present in the location. PCR+/NGS- or NGS(artifacts) mean you are using the incorrect primers (all incidents happened with the PREDICT ORF1ab only primers). archive.md/rj1pV PCR-/NGS+, especially when archive.md/csYBM the primers are aligned over by NGS reads, indicate that the samples have been catastrophically contaminated as NGS is a more complicated process that are far more prone to contamination compared to PCR. archive.md/13bdP The stall for Q37 is negative at Jan 01. They then went on sampling the same stall including the “freezer” twice afterward, attempting to verify the “sample” they considered most promising. Bringing in artifacts elsewhere and samples without a read (the only sample with a real SARS-CoV-2 read at all gathered using the PREDICT ORF1ab only primer pair was a sewage well connected to the municipal sewage system on the exact opposite to the “wildlife corner”.), but never SARS-CoV-2 reads any more. This fact is also reinforced with the intriguing observation where Q37 is found to be in the same correlational series between SARS-CoV-2 and Homo Sapiens as other Q* samples. Attempts at sampling the archive.md/FskYn archive.md/gvHfw archive.md/4cCHG archive.md/csYBM archive.md/rj1pV “storehouse” just ended up with a total catastrophe—archive.md/13bdP the sampling team brought in in-lab culture contaminants, not even aged for more than a day, into the sampling sites again when they suited up in their lab and entered the location. Impossibly fresh intracellular Homo Sapiens and SARS-CoV-2 transcriptomes, neither capable of lasting for more than two days ex-vivo in that condition, ended up contaminating the samples and without a single read of a susceptible animal inside those “samples”. Sampler contamination and cross-contamination. archive.md/LJzSO archive.md/VNr75 Never an infected vendor or animal. In fact, the samples in the market follows the rule which a positive sample archive.md/CTP3i archive.md/ETjzS archive.md/BWZJL must be contacted by samplers.🥼👖👢=positive. archive.md/NeybM archive.md/2PM9Y archive.md/RirQ7 And not frequently handled by vendors.🥩🥬🍄🛁📻☎️📦🧺=negative. archive.md/HlJ9o archive.md/nAqKp archive.md/rSaO9 They also put bleach onto the toilets and the mahjong room before sampling them. This is a clear move to cover up. archive.md/csYBM And no the “stall” W5-NA was sampled on the inside 27/01/2020, negative. (Not even animal CoVs were there) The toilets is the real contamination source. archive.md/C5oal archive.md/RSsS7 and yes only Homo Sapiens positively correlated with SARS-CoV-2 consistently in all metrics, or formed any kind of line or grouping pattern at all that allow the abundance of one to be estimated at above-random success rate and precision using the other (e.g. have any significant mutual information with SARS-CoV-2). archive.md/0O2TN archive.md/GjlEx

@NestCommander - Kevin W. McCairn PhD

If you examine the inside of “wildlife stall A”, then all you see is boot prints and suit marks. None of it is animals. All metrics now favor Homo Sapiens as the most likely source of the SARS-CoV-2 sequences there. The wildlife stalls all sold susceptible animals. They only sampled wildlife stalls in Jan 12. Then positives are found closest to the toilets because that is where contaminated suits and boots most likely rub trample and kick. No different from W4-28 and W4-26-28, really. Not forming a line on the correlation diagram=no mutual information=spurious. That is why it dissolved completely when asking “which species shed the SARS-CoV-2” in slices where the analyte concentrations aren’t 0. That is also why they fraudulently bleached the toilets before sampling them.

@NestCommander - Kevin W. McCairn PhD

And this is how you ID a spurious result. If the correlational diagram show a neat line indicating an consistent increase in the count of one candidate factor as the target analyte increased, it indicate that the target analyte is probably caused by the candidate factor with a consistently raising minimum of the factor per analyte suggesting that all of it is brought in alongside this candidate. If the correlation diagram show an randomized pattern or even a line of negative slope, especially when nearly all of the target analyte is found in one place, then it is probably that it is just the one place have one or few potential candidates that are less abundant elsewhere, which with the 25+ candidates in the market sample correlation analysis guarantee 1-2 for every stall (and most of which are on the ground just like anything trampled from the toilets, with entirely different reasons). If you can not use the concentration of the target analyte to reliably predict the concentration of the candidate, or come up with an result that the more target analyte there is the less candidate there is where the target is found, e.g. an absence of or negative mutual information, then it is most likely spurious and extremely unlikely that candidate yielded or is brought alongside the target analyte. Causation are bijective. Confounders are injective. Spurious correlations are correlated only in some metrics and slices but not all. Inconsistency between different slices and metrics indicate an lack of true causation and likely confounder that makes false positive in some but negative in the other. A consistent positive correlation in almost all metrics and no negative correlation in any metric, Like Homo Sapiens, indicate that there is true causation that some disruption may have occurred. Species that have “positive” correlation only in some metrics out of a single slice, (not even all the slices examined for that date), but negative or zero in all the other metrics and slices with the mutual information metric yielding negative and zero only regardless of slice, like oriental rat snake or malayan porcupine, are spatially confounded—they are “the most unique species found in the 1 stall at that slice that was closest to the toilets”, and since every stall have one such species, they represent false discovery by lottery fallacy, and fails when any other slices are used. They also failed to form a line on the correlation plot which indicate that there is no causation and the animal did not shed the virus where it was found.

@NestCommander - Kevin W. McCairn PhD

archive.md/csYBM And no the “stall” W5-NA was sampled on the inside 27/01/2020, negative. (Not even animal CoVs were there) The toilets is the real contamination source. archive.md/C5oal archive.md/RSsS7 and yes only Homo Sapiens positively correlated with SARS-CoV-2 consistently in all metrics, or formed any kind of line or grouping pattern at all that allow the abundance of one to be estimated at above-random success rate and precision using the other (e.g. have any significant mutual information with SARS-CoV-2). archive.md/0O2TN archive.md/GjlEx This result is consistent with the theil-sen estimator, which explicitly measures mutual information through the use of all slopes in the data points, which measures the predictive power of the other data points and one metric of one data points to the other metric of the data point. You can easily distinguish between common tertiary cause (confounded) from true causation by looking with increasingly finer grain of resolution, especially where the data points aren’t 0. Unlike spurious correlations from Confounding factors which ends at the resolution where the factor acts on, True causation stay correlated in every resolution and in any set of data points especially where the data values aren’t 0. In fact, confounding factors often crash in correlation quite early before that. “Do pirates get less prevalent the hotter it is when normalized agains temporal trends, where many things have correlation with time”? Is the same as “Do SARS-CoV-2 correlate with animals at all when normalized against confounding pathological distribution of insufficient spatial spread, which each stall including one closest to the toilets have several separate unique species?

@NestCommander - Kevin W. McCairn PhD

The “perfect synergy” is a lie.

@NestCommander - Kevin W. McCairn PhD

And it really only need “VERO cells and HAE cultures” to adapt to this.

@NestCommander - Kevin W. McCairn PhD

What those analyzers think: “many different precise and specific pieces recombine into SARS-CoV-2”. Reality: an unpublished but readily sampled SARS-CoV-2 progenitor spread fragments over time into multiple locations. Some end up in the published samples. Assumption of recombinant origin is almost always because of a lack of published samples sufficiently close, especially with multiple of samples of comparable distance. One specific contig is fished out of the assembly graph from a bucket of reads on the criterion that it meet the RE site requirement, before being used for cloning. Natural recombination will not pick that specific contig over any other possible assembly graphs for spillover. What they claim: “Only QTQTNS + PRRA would work!” Reality: they test an appropriate human FCS on all manner of Spikes, especially when they see an S1-S2 site “cleavage sites in S2” (that are structurally homologous to 757/900 for HKU1 “other coronaviruses”) with the example of “667/792 for SARS” that have deviated from the established consensus of spillover-capable SARSr-CoVs up to the point if DEFUSE. And it just happened that the closest human FCS to the first mismatched S1-S2 they see is the hENaC FCS, introduced by adding “ARRA” to “RSVAS”. Several versions tested and one ended up working. https://gab.com/Flavinkins/posts/109205261283826972 ReCCA is tautological and fictitious. https://gab.com/Flavinkins/posts/109863181504837302 https://gab.com/Flavinkins/posts/109465063042828622 https://gab.com/Flavinkins/posts/109255356915252021 BtSY2 is sequenced in 2018. https://gab.com/Flavinkins/posts/109399710986742685 BANAL is in the hands of the DOD in 2017. https://gab.com/Flavinkins/posts/109340247585238829 https://gab.com/Flavinkins/posts/109800300869616862

Flavinkins on Gab: 'https://gab.com/Flavinkins/posts/1089460581208001…' Flavinkins on Gab: 'https://gab.com/Flavinkins/posts/108946058120800199 How would you go onto constructing a synthetic consensus backbone that would be 1: guaranteed to be rescuable. 2: easy to manipulate as fragments. 3: easy to manipulate even after being ligated? You would need to choose fragments with type IIS restriction sites such that 1: each individual type IIS site must have a precedent in its presence of absence in the wild in at least 1 Sarbecovirus (distance doesn’t matter here. Can be as distant as BM48-31. Abundance matters statistically (rarer individual sites are less likely to show up at spillover) but does not matter if you are making a clone. As long as you find your desired presence/absence of a site at a specific location in even just one genome, you can use it and be confident that it won’t break your consensus genome. This is governed by both the availability of individual side precedence in sampled genomes and by the location of the site, whatever that doesn’t break the genome and is optimal for cloning gets chosen) to ensure that highly conserved RNA structural motifs aren’t disrupted. 2: there must be no BsaI sites between two BsmBI sites and no BsmBI sites between two BsaI sites to minimize the need of double digestion. 3: the number of overly small fragments should be minimized while no overly large fragments should be present at all. That is, the Standard Deviation (S.D.) Of the length of the fragments shouldn’t be too high. 4: the number of fragments should be kept to as low as possible with the restriction that you can easily manipulate each individual fragment within a M13/pUC vector backbone, as an excess number of fragments are more difficult to keep track of or manipulate. The type IIS restriction sites in the SARS-CoV-2 genome matches all the requirements above. Other genomes in its clade (or even any that is found in Asia)? Not that much. Unfortunately since clonability does not translate to spillover potential (without a lab), the fact that easily clonable Sarbecovirus genomes are rare in general also translates into the possibility that one that ended up spilling over just happen to be one that is easily clonable as SARS-CoV-2, being low. Since the fragments are what that is being chosen for the consensus genome, site selection influences ReCCA especially for the segments that were located near restriction sites—the segments surrounding them are what that were originally chosen in the consensus construct, where one of the requirements used is that the pattern of type IIS sites within the selected set of fragments should make the final genome assembled from them easy to clone. Finally, how could all the non-SARS-CoV-2 genomes you use in the ReCCA graph have a BsaI site in the first position of the F3 fragment (this site is highly conserved in Sarbecoviruses found in Asia) , but the final ReCCA, supposedly generated from related sequences sampled in the wild, not having that site? Either the ReCCA algorithm itself have considered these sites as part of the synonymous sites that was used to infer the “highly conserved segments”, and included SARS-CoV-2 itself into the analysis (making the algorithm tautological), or it is the result of the consensus-generation process where the choice of segments and sites to be used for the consensus included an requirement for the ease of cloning and manipulation for the final genome—something that would matter if you are synthesizing and rescuing it in the lab, not so much for anything that “spills over from the wild”. https://archive.ph/VypuD' gab.com
Flavinkins on Gab: 'More importantly, when considering the current RG…' Flavinkins on Gab: 'More importantly, when considering the current RGS systems, it turned out that none of the supposed ReCCA components are compatible with the cloning scheme that somehow were identical in their RE site order and REase use between U.S. and Chinese publications—the 2 BsaI sites within the ORF1a strongly interferes with the F3 fragment of the cloning scheme and when these sites are absent in a few select genomes, interfering sites pops up elsewhere in the genome that nevertheless still make the systems unworkable. The SARS-CoV-2 genome is the only one of which both the conventional and the no seems type IIS cloning methods would work with BsaI/BsmBI for the assembly of the genome—allowing both the easy assembly and ease for revision of the genome in all labs that were involved in the DEFUSE program.' gab.com
Flavinkins on Gab: 'In fact, how the ReCCA algorithm functions (it pi…' Flavinkins on Gab: 'In fact, how the ReCCA algorithm functions (it picks the closest sequence to the SARS-CoV-2 branch On “phylogenetic trees constructed on each (very short) segment of the genome”) mean that it is impossible to reconstruct an ReCCA without inputing the SARS-CoV-2 genome into the algorithm (as the reference genome to be aligned against) and thus biasing the result catastrophically—to the point that it is impossible to distinguish this process from what that would be used during the construction of an consensus genome for an infectious clone (fragments are picked with a requirement that the sites on the selected fragments lead to a genome that can be easily synthesized and constructed), and removes any statistical power of “ReCCA” to argue that the specific type IIS restriction pattern of SARS-CoV-2 to be the only possible combination of sites that is “evolutionarily likely”. It once again, failed to provide any biological reason why a specific, <1-in-100, easy-to-clone pattern being any more likely to end up being the spillover strain (that must not use the SARS-CoV-2 genome itself as the reference when such probability is assessed) compared to the >99-in-100 other possible combinations outside the S1 With the “ReCCA components” (where none were easy to clone by themselves) that are not easy to clone, in a non-circular manner. https://gab.com/Flavinkins/posts/109288626916761348' gab.com
Flavinkins on Gab: 'When a synthetic recombinant genome for a coronav…' Flavinkins on Gab: 'When a synthetic recombinant genome for a coronavirus is constructed, fragments are selected from relevant natural bat isolates with a requirement that the type IIS restriction pattern using the planned enzymes on the resulting assembly should enable easy cloning and efficient manipulation—a less than 1 in 100 chance for this to happen randomly by chance for recombination outside the S1 region of the genome, and completely irrelevant to spillover. When a natural virus spills over, there is very little effect (within 1 order of magnitude) on the chance that some specific strain would end up becoming the pandemic strain for recombination ancestry outside the S1 region. There is no requirement that a strain that spills over must be a strain that is easy to clone by the 2 most popular type IIS restriction enzymes that were used in CoV genome assemblies, and the chance given natural spillover of an ancestry that had an efficient type IIS RGS system without modification is the same chance as finding one such strain in nature just by 1 single random sampling—so far no specimen from Asia satisfy this on their individual genomes. Again, which sequence on the ReCCA graph were not sampled from nature? Unfortunately, the so-called “natural recombination ancestry” argument may well just be one of the many ways workable coronavirus genomes are “recovered” from a set of otherwise unisolated samples. Whatever you reconstruct out of natural isolates for a clone, it must be easy to clone. It can be from one of the rare samples you find with an easy-to-clone pattern, or it could be one of the combinations of various contigs from sequencing a pooled sample. It could also be a chimeric genome constructed using fragments selected from related wild isolates with a requirement that the result is easy to clone. When a strain spills over naturally, there is no requirement that it must be easy to clone—restriction enzymes work on DNA not on RNA, and there is no reason why the specific combination with an easy to clone site pattern must be selected other than the posterior claim “it happened” (ReCCA construction used SARS-CoV-2 genome as reference). This is a circular argument as the claim that “the SARS-CoV-2 genome with its unusual combination of type IIS sites is the result of a natural spillover” assumed P(spillover|strain have good site combination)>=0.5 while P(strain have good site combination)<<0.5 with the only justification “we observe that the SARS-CoV-2 genome is easy to clone and can be constructed using a combination of fragments from some 8+ different “bat virus strains”” only able to justify this implied probability assumption with the assumption “SARS-CoV-2 is the result of a natural spillover”, a hypothesis that is being tested in the type IIS RE site analysis paper in stead of an underlying assumption. In conclusion, while a ReCCA with an easy to clone type IIS site combination with the go-to enzymes used for assemblies of this length is a possible combination of the bat coronavirus sequences known from sampling, there is no justification for this hypothetical and still unsampled ReCCA to be the only possible combination where a spillover is possible or that a spillover strain will have a probability that it will be easy to clone being >0.5 while the chance of finding such a strain from a random sampling from the wild being only about ~0.01.' gab.com
Flavinkins on Gab: 'Additional sample formats, such as “bat coronavir…' Flavinkins on Gab: 'Additional sample formats, such as “bat coronavirus isolate NNNNNN” are found in 2015 samples, whereas the 2016 sequences (the last sequences to be deposited into GenBank from known bat coronavirus searching papers in China/CAS) contained within their authors “Edward C Holmes”. This may hint on the role of E.Holmes on the handling of bat Coronavirus sample sequencing for samples collected “between 2015-2019”. Sequences MH315932-MH315944 have formats “XxNNNNNN”, however these contained samples from as early as 2013, making it difficult to ascertain as where these samples came from. However, these numbers (and the “Spread and Geographic Structure of SARS-related Coronaviruses in Bats and the Origin of Human SARS Coronavirus” paper, with E.Holmes in the GenBank records) contained the only deposited CAS samples known to be collected in 2016, which could be taken as evidence of E.Holmes and WIV collaboration for sampling efforts and sequence/sample sharing, in the 2015-2019 period. A collaboration (sample/sequence sharing) between Guangdong and Wuhan institutions through E.Holmes https://zenodo.org/record/6849652#.Y38toiW8klT , can not be ruled out. The original GenBank deposition date of 2018 seems to coincide with the supposed sampling date of “BtSY2”. We now know that some of the samples taken in 2018 under E.Holmes contained RBD sequences related to the Omicron strain of SARS-CoV-2. The question becomes: Why you are taking samples from bats (dissected rectum), without performing some kind of tests immediately after sampling, as early as in 2015-2018, given that an ethics approval for the destructive sampling of wild animals in China required some kind of academic program to be first sent for approval, which have to include the detail of the exact experiments that requires the dissection of the bats and collection of the rectum samples. If any kind of testing/screening/experimentation were done initially on the samples (immediate experimental plan would have to be provided for the sampling proposal to be justified and approved—and “for storage, until some technology that doesn’t yet exist until late 2021” isn’t one of them), what were the results? Why, despite sampling of bats by EHA/CAS and expeditions into Yunnan/Mojiang mine continued all the way until 2019, the last such sequences deposited from China was sampled in 2016? If “This research, including the procedures and protocols of specimen collection and processing, 262 was reviewed and approved by the Medical Ethics Committee of the Yunnan Institute of 263 Endemic Diseases Control and Prevention. (No. 20160002).”, why weren’t the results immediately made available to the public as the samples were collected and processed over the course of 4 years? RNA samples are after all, very fragile and does not tolerate long-term storage very well. When were these actually sequenced? (I see 15 different sequencing machines in the FCIDs, with FCIDs ranging from H2 to H7, HG to HY, and sequencing on the same machines hundreds of runs apart. This could indicate sequencing done immediately after sampling) And if these were sequenced before the pandemic, who had access to the sequences? Were these really “newly discovered” viruses, or just sequences that were put into embargo in the “great silence” of CAS accession numbers between 2016-2019, only recently released? (Should these samples be sequenced pre-pandemic, near their collection date in 2018, this could be corresponding to phase 1(QS0) of DEFUSE. After sequences generated as early as in mid-2018, there would be more than 1 year to work with the cloning and culture experiments.) The machines found in https://github.com/Augustpan/Individual-Bat-Virome/blob/main/raw_data/lane_id_table.csv Are: A01426 A00821 A00920 A00270 A00877 A00289 A00881 A00783 A00253 A00917 A01045 A00808 A00459 A01415 A01050 The samples with BtSY2 are: S18CXBatR24 @A00917:648:H3Y25DSX2:4 S18CXBatR29 @A00783:739:H3V32DSX2:3 Both appeared to be on the earliest run on that particular machine, where the run contained no sample from 2019, and where the flow cell ID were from an early era (H3). Archive for read mappings: https://archive.ph/CuzzR Archive for lane IDs: https://archive.ph/IKxD1' gab.com
Flavinkins on Gab: 'To see more about the DOD-sponsored Institut Past…' Flavinkins on Gab: 'To see more about the DOD-sponsored Institut Pasteur sampling of the BANAL caves in 2017 and the censorship of even the already-sequenced batflies library https://gab.com/Flavinkins/posts/109150240613656613 https://gab.com/Flavinkins/posts/109139121799069783 https://gab.com/Flavinkins/posts/108782000872829271 http://gab.com/Flavinkins/posts/108745003276913992 https://gab.com/Flavinkins/posts/108938621623162894 https://gab.com/Flavinkins/posts/108961381086722860 https://gab.com/Flavinkins/posts/108808844066927838 https://gab.com/Flavinkins/posts/109198525541365424 https://gab.com/Flavinkins/posts/109222447665307488 PREDICT-2 and EHA activity in SE. Asia including Southern Laos https://gab.com/Flavinkins/posts/109079936391006382 https://gab.com/Flavinkins/posts/109079963106312117 Past lab escapes from IP france https://gab.com/Flavinkins/posts/108929427082821215 And on the type IIS REase found in IP cambodge https://gab.com/Flavinkins/posts/109215673255176764 https://gab.com/Flavinkins/posts/109216529429569399' gab.com
Flavinkins on Gab: '@Graviola_Finland @EIGARBARINO https://archive.m…' Flavinkins on Gab: '@Graviola_Finland @EIGARBARINO https://archive.md/8rlbT FULL TRANSLATED TEXT of the briefing of the Ministry of Defense of the Russian Federation on the analysis of documents related to military biological activities of the United States, made on January 30, 2023 with the supporting documentation that it provided. https://gab.com/Flavinkins/posts/108782801366501491 https://gab.com/Flavinkins/posts/108908816879287433 https://gab.com/Flavinkins/posts/108808844066927838 https://gab.com/Flavinkins/posts/108808523459345532 It confirms that sampling and researching effort for S.E. Asia is in deed being conducted in these labs. Anomalies regarding the Caspian sea in 2019: https://gab.com/Flavinkins/posts/108949034317465342 EHA activity in the Caspian sea region, up to 2019: https://gab.com/Flavinkins/posts/108782587895528182 https://gab.com/Flavinkins/posts/109139121799069783 https://gab.com/Flavinkins/posts/109529211058973737 DOD, IP laos and batflies🦇🪰: https://gab.com/Flavinkins/posts/108961381086722860 https://gab.com/Flavinkins/posts/108938621623162894 https://gab.com/Flavinkins/posts/108782000872829271 https://archive.md/JZkwi EHA, Laos and PREDICT-2: https://gab.com/Flavinkins/posts/109158749548555994 https://gab.com/Flavinkins/posts/109079936391006382 https://gab.com/Flavinkins/posts/109079963106312117 https://archive.md/hMp7x 🦇🧪🥣? https://gab.com/Flavinkins/posts/109150240613656613 https://gab.com/Flavinkins/posts/109198525541365424 https://gab.com/Flavinkins/posts/109222447665307488 https://gab.com/Flavinkins/posts/109728264957247673 https://archive.ph/3fFfn https://archive.md/T6sN5 (No wildlife trade route linking Wuhan to Laos…… But plenty of sampling route linking Laos to labs both in Wuhan and on the East Coast……)' gab.com

@NestCommander - Kevin W. McCairn PhD

Remember that time you say it must be S2’?

@NestCommander - Kevin W. McCairn PhD

It is not just that SARS-CoV-2 Wuhan grows best in VERO cells out of all variants.

@NestCommander - Kevin W. McCairn PhD

Some earliest patients harbored inside their QS specific S1-S2 deletions that can form only in VERO E6. https://gab.com/Flavinkins/posts/109640519028841414

Flavinkins on Gab: 'https://journals.asm.org/doi/10.1128/JVI.00790-20…' Flavinkins on Gab: 'https://journals.asm.org/doi/10.1128/JVI.00790-20 This is not the only cell passage specific deletion that would end up in some of the very earliest patients. https://academic.oup.com/cid/article/73/2/e437/5869859 DelQTQTN, a variant that emerges during VERO cell passage, is found specifically within at least 3 patients arriving from Wuhan in Guangdong, and variations at the “upstream probe binding site” (where closest related QTQTNS-bearing genomes contained only one transition at this probe, insufficient to prevent its binding), which also emerges at cell culture passage of SARS-CoV-2 in VERO cells, are found within patient samples taken from the Central Theater Command Hospital in Wuhan, alongside with variants that had deletions in the SPRRARS site, del-mut-1. DelQTQTN(which is specifically found within the patient samples and where the deletion is exactly 1AA longer than those claimed to be in RmYN02/RacCS203/Banal-247) and variations in the upstream probe binding sites are unique to VERO cell cultured strains of SARS-CoV-2. Their presence within the earliest patient samples within China implies that a VERO cultured stock was the proximal inoculum of many of the earliest patients within China. This directly point toward the proximal origin of the Chinese outbreak being associated with virology research and culturing of viruses.' gab.com

@NestCommander - Kevin W. McCairn PhD

archive.md/HlJ9o https://archive.md/rSaO9 https://archive.md/13bdP https://archive.md/nAqKp They also put bleach onto the toilets and the mahjong room before sampling them. This is a clear move to cover up. https://archive.md/rj1pV https://archive.md/FskYn archive.md/csYBM And no the “stall” W5-NA was sampled on the inside 27/01/2020, negative. The toilets is the real contamination source. https://archive.md/LJzSO https://archive.md/4cCHG archive.md/C5oal archive.md/RSsS7 and yes only Homo Sapiens positively correlated with SARS-CoV-2 consistently in all metrics, or formed any kind of line or grouping pattern at all that allow the abundance of one to be estimated at above-random success rate and precision using the other (e.g. have any significant mutual information with SARS-CoV-2). archive.md/0O2TN archive.md/GjlEx What they tried to hide with this: The fact that “closest to the toilets” is the only factor that governs where you are going to see positive samples the most in the market—there is no difference between W4-28 and W4-26-28 in 01/01/2020 and W6-29-33 in 12/01/2020 in term of where the virus came from and why they have the highest positive sample count out of all sample counts in their respective sampling runs. Sampler contamination and cross-contamination. archive.md/LJzSO archive.md/VNr75 Never an infected vendor or animal. In fact, the samples in the market follows the rule which a positive sample archive.md/CTP3i archive.md/ETjzS archive.md/BWZJL must be contacted by samplers.🥼👖👢=positive. archive.md/NeybM archive.md/2PM9Y archive.md/RirQ7 And not frequently handled by vendors.🥩🥬🍄🛁📻☎️📦🧺=negative.

@NestCommander - Kevin W. McCairn PhD

Unfortunately, none of the “drain” samples contained actual SARS-CoV-2 reads. They have animal CoVs, demonstrating that the SARS-CoV-2 in the market isn’t present in animal tissues and does not last past 12/01/2020 as legitimate reads, and that are also happens to be exceptionally cross-reactive to the ORF1ab only tests used.

@NestCommander - Kevin W. McCairn PhD

Unfortunately: The actual samples from the “drain systems of the Huanan market”—included not even a single read of SARS-CoV-2, but only animal CoVs like HKU14 or HKU31 which are known to cross-react with the PREDICT primers. These were also the only CoVs identified by independent sampling in 02/2020. The only read of SARS-CoV-2 from these drains/sewage wells—is found on the opposite corner of the market and is in the main system where the toilet flushings meets the drainage system in the pipes (note: any spillage or other exit path of the “overflowing sewage” from the toilets into the market, as specified by descriptions in media reports, are still going to leave in the area of the toilets.), and where other sources of the virus in the communal sewage system enters the sewage wells connected to the market through the sewage pipes themselves. https://assets.researchsquare.com/files/rs-885194/v1/78e0e6ce-4a76-48de-9f5c-76bab452bbe6.pdf?c=1665607885 in fact the nature of SARS-CoV-2 here being superfluous contamination that have an origin in human secretions and cell culture supernatants brought into the stalls by the samplers and on boots, shoes and suits is obvious here. Independent sampling indicate that only the animal viruses highly correlated with the animals were left in February. The SARS-CoV-2? Superfluous, in human metabolic products and secretions, and not in butchered tissues. They are cleaned off and degraded efficiently. The animal native viruses are inside the tissues and are highly persistent—class of contaminant type different and SARS-CoV-2 is in the class that is inconsistent with the behavior when pitched against cleaning than animal CoVs (which unlike SARS-CoV-2, the animal viruses including CoVs persisted in tissue fragments of animals generated from butchering and animals banging against cages for more than one month, whereas all the “SARS-CoV-2” after 12/01/2020 are either amplicon artifacts, PREDICT primer false positivities (no reads at all), or evidently freshly prepared cell cultures which even the fragile mitochondrial transcripts of the Homo Sapiens and intracellular viral transcripts of the SARS-CoV-2 have been preserved (they decay within a day after loss of cell viability) and where no non-human mammals at all were present. (Clearly a recent cell culture added into these “storehouse” samples). https://archive.md/13bdP archive.md/FskYn archive.md/gvHfw archive.md/4cCHG archive.md/csYBM archive.md/rj1pV

@NestCommander - Kevin W. McCairn PhD

Cold hard reality: there is nothing special to W6-29-33 compared to W4-26-28. Closest to the toilets is what that govern where the virus would be found. Fact: w6-29-33 and the market supply chain did not get shut down at all. The former operated as “金秀山家禽批发商行” up to the end of 2021 and the latter operate still to supply other markets in Hubei, archive.md/yyX0Z archive.md/iw1Pz archive.md/4rVph archive.md/DChUL evidenced by the fact that these suppliers including the exact animals sold were readily sampled in Jan-feb 2020. This included also specific sampling of several species inside the stall itself, also negative. Their animals tested negative and no outbreaks happened. They represent the full inventory of raccoon dogs and siberian weasels in the market. The Hubei supply farms then represent the full inventory of many other species. The only Yunnan supplied animals all landed on the wrong stalls, testing negative even when sampled as is in the market. archive.md/e3615 archive.md/vWjZl

@NestCommander - Kevin W. McCairn PhD

This is the reality: VERO cell adaptation is max in Wuhan compared to bat or VOCs.

@NestCommander - Kevin W. McCairn PhD

And this picture is worth a thousand words—“VERO cells and HAE cultures” adapt the virus like this exactly.

@NestCommander - Kevin W. McCairn PhD

And also fact: you really can not 1: ignore the 50+ strains of bat SARSr-CoVs identified by the EHA, still not published—anything sufficiently close won’t be “mosaic recombinant”. And 2: the China claim that there were no cases before the market got completely self-debunked when they then went on saying that 67 samples in an 2021 Wuhan of >4% seroprevalence “tested all negative”. Numerous anomalies leaked in regard to early detections that is incompatible with a market origin. Sinterklaas/Faucier: Wei Jingsheng: WHU student reports: First public Wechat Spike in “SARS”: Wuhan lab banned from being mentioned the first official declaration(nobody is allowed to doubt the constructed official narrative): Callahan: Murdered Zhou Yusen with body bags found outside the WIV: It is impossible to have 67 wuhan residents sampled in 2021 to test all negative for SARS-CoV-2 antibodies. Blatant ben HU lie on “not working with SARSr-CoVs” Still operating illegal labs in the U.S. They screwed up with the military games it seems. And it is not unusual to see samples in China being sequenced only months to years after initial collection and culturing. Hong kong suspend Wuhan mail over Nov-Dec 2019. https://gab.com/Flavinkins/posts/108890415550666278 Chinese communities in Italy show evidence of prior immunity. https://gab.com/Flavinkins/posts/108952233844548674 Mean time of earlier SARS-CoV-2 related discoveries=mid November 2019. Clinics—even in mid December 2019 in central Wuhan—got 10 times more cases than usual in mid November and only worsens through December and January. https://archive.md/VXtu9 Cases begun to take off and it have reached “more than 600 a day” just before the start of 2020 that they completed all of their preparation job and got all their PPE they can get before 01/01/2020. Then ascertained cases alone flooded the hospital just about 10/01/2020. https://gab.com/Flavinkins/posts/109248812361151175 https://archive.md/VXtu9 Here is evidence that widespread silent outbreak have already happened inside Wuhan before it reached the HSM, with the Tongji and Union hospitals already seeing novel coronavirus inside the Wuhan CBD in 6-8 Dec 2019. Of course this is again never official. Why trust only the official cases data with a known bias and severe censorship? https://archive.md/UIBkB

Flavinkins on Gab: 'https://archive.ph/teJqh Archive from @florin_unc…' Flavinkins on Gab: 'https://archive.ph/teJqh Archive from @florin_uncovers More and more: Hong kong mail suspended all express mail services to Wuhan in 15/11/2019 due to “air transportation arrangements” This is then brought out by a news article in 02/01/2020 denoting “multiple cases of unknown pneumonia in Wuhan” (and the first patient returning from Wuhan after the first official reports from the city). https://web.archive.org/web/20200913130923/https://topick.hket.com/article/2532398/香港郵政:往武漢特快專遞航班去年11月16日起取消%E3%80%80故暫停服務 https://archive.ph/dsJ15 https://archive.ph/6LuXg https://archive.ph/Vt4mQ What this may have resembled? Also Wuhan doctors. https://archive.ph/VXtu9 https://web.archive.org/web/20220312073852/https://www.nzherald.co.nz/world/covid-19-coronavirus-wuhan-doctor-tells-what-it-was-really-like/6Q23B4ISGLPT7U7QC34G4VDAA4/' gab.com
Flavinkins on Gab: '@Biorealism “During an extraordinary period in De…' Flavinkins on Gab: '@Biorealism “During an extraordinary period in December, it was necessary to place deceased individuals outside of the designated morgue, in a secure room, for a short period of time,” it read. “The Ottawa Hospital has converted spaces, formerly used for autopsies, within the morgue to manage unexpected surges in demand. Conference rooms and ward beds are not used for housing deceased individuals,” the statement continued.“ https://www.ctvnews.ca/canada/ottawa-hospital-denies-bodies-were-left-in-conference-rooms-as-morgues-overflow-1.4756984 https://web.archive.org/web/20200108025449/https://www.ctvnews.ca/mobile/canada/ottawa-hospital-denies-bodies-were-left-in-conference-rooms-as-morgues-overflow-1.4756984 If you are to check the date for this, the announcement was in January 2020. https://genomemedicine.biomedcentral.com/articles/10.1186/s13073-021-00986-9 Note that there were no introductions from China traced for the later (recognized) Montreal and Quebec outbreaks. What could these excess bodies be? https://academic.oup.com/cid/article/72/12/e1004/6012472 Table 1 in this paper should be considered important.' gab.com
Flavinkins on Gab: 'https://archive.ph/yu2Jg Now, see this. Remember …' Flavinkins on Gab: 'https://archive.ph/yu2Jg Now, see this. Remember that “中部战区总医院”? “ 2019年12月31日,武汉市卫健委首次公开发布通报,确认多例肺炎病例。   与此同时,中部战区总医院发热门诊人数陡增,最高时一天超过600人。江晓静、王琼书、刘孟丽等一批中部战区总医院专家,感觉到病情凶险。   作为全军急性呼吸道传染病病原监测参比实验室,中部战区总医院负压实验室是驻汉部队唯一带有负压功能的实验室。从2015年起,为监测流感、呼吸道腺体和其他传染病,他们每年冬春季都会紧密监测发热患者情况,并进行跟踪和采样。   但今年的数据变化太快太突然,没有任何征兆。   经中部战区总医院感染科、疾控科等科室专家和医护人员共同讨论研究,提议马上启动《呼吸道传染病防控方案》。中部战区总医院党委专题召开分析会,形成共识。   医院成立领导小组,主官亲自挂帅;抽调精兵强将,核心部门全员参与;对发热门诊、传染科等关键环节开展防护培训,采取隔离措施,提高防护等级;加紧储备口罩、防护服、隔离衣等防护器材,紧急采购30个正压呼吸器和60个备用滤芯。同时,向驻汉驻鄂部队进行传染病防护提醒。   今年元旦,中部战区总医院进入临战状态。   1月4日,医院调整扩大发热门诊,全院提高一级防护等级。   1月6日,开始收治第一例患者。几天后,传染科床位告急。   1月15日,医院决定火速扩建传染病区。   1月16日,向武汉市卫健委送检第一例样本病例。   1月17日,抢建的传染2病区、3病区开放。   1月19日,医院提升疫情防控指挥等级,成立一线指挥部,党委成员集体住进办公楼;机关各部门重新进行人员编组和任务分工;专家组、医疗组、保障组以及各预备队抽组完毕;全院进行传染病防治专业培训考核。” Then “新冠肺炎被纳入乙类传染病、采取甲类措施严格管理。而中部战区总医院发热门诊从一开始就采取了高一级的防护措施,严格按照甲类传染病进行处置和管理。   随后,疑似病例数、确诊病例数、死亡病例数不断攀升,治愈人数却始终显示着“0”。” Keep in mind, the listing of “Novel coronavirus infected pneumonia(NCIP)” as a “class B infectious disease” is in 20/01/2020. Standard 1, from 15-17/01/2020, and the “试行诊疗方案” before it, require “unsuccessful antibiotics treatment” and “unsuccessful treatment using a panel of “standard antibiotics”” for cases that didn’t have exposure history to the Huanan market. This mean that most if not all the cases that can be ascertained as being “NCIP” or “VPUE” by the standards at that time have to be in the severe non-self-limiting group. 06/01/2020 is exactly 3 days after they begin case ascertainment according to the “试行诊疗方案” they received in 03/01/2020, through the use and monitoring of antibiotics treatment on fever patients. Just after “a few days” (“3-5 days”) of they begin to ascertain cases for isolation, “the infectious disease wards begin to run out of beds”. So many fever patients floods the hospital that they begin to “expand the fever clinics” at 04/01/2020. And what caused them to begin “今年元旦,中部战区总医院进入临战状态”? “Enter battle stations at 01/01/2020”? “On December 31, 2019, the Wuhan Municipal Health Commission issued a public notice for the first time, confirming a number of pneumonia cases.   At the same time, the number of fever outpatients in the General Hospital of the Central Theater has increased sharply, with more than 600 people a day at its peak.A group of experts from the General Hospital of the Central Theater, including Jiang Xiaojing, Wang Qiongshu, and Liu Mengli, felt that their condition was dangerous.   As a reference laboratory for the monitoring of the pathogen of acute respiratory infectious diseases throughout the army, the negative pressure laboratory of the Central Theater General Hospital is the only laboratory with negative pressure function of the troops stationed in Han.Since 2015, in order to monitor influenza, respiratory glands and other infectious diseases, they have closely monitored patients with fever every winter and spring, and followed up and sampled them.   But this year's data changes too quickly and suddenly, without any signs.” Indicated by the ENA reservation dates, this begun at least on 10/12/2019. Out of all the cases that they accept into their isolation wards, “suspected cases, confirmed cases and deaths keeps raising up, but “recovered cases” remained 0”. All isolation ward/infectious disease ward cases were ascertained according to the standards that were then official in Wuhan. Self-limiting cases were excluded. This is also how WH01-WH04 were sent to the BGI. Samples were saved from all fever and respiratory cases admitted to the hospital, and when they received a command for “continued epidemiological investigation in several hospitals (near Huanan market), Huanan market and the neighborhood of Huanan market” in 31/12/2019, 4 cases from the hospital that satisfied the “from or near the market” criterion were selected, and samples sent for analysis at availability (when BALF sampling from bronchoscopy is performed). Only WH01 with a sample that is available in 26/12/2019 would be reported to the WMHC and enter the WHO dataset, as the military commanded General Hospital of the Central War Zone seems to be only disclosing case data to other institutions after they have a result on the cases first (where the first case ascertained with the “试行诊疗方案”, sometimes in 06/01/2020, only had the sample sent to and reported to the WMHC in 16/01/2020.). WH03 reported in Zhongnan. Confusion on the identity and nature of WH01-WH04 would continue even until today. https://zenodo.org/record/4119263/#.Y1yAtCW8klQ At its peak, the General Hospital of the Central War Zone were receiving 600 cases a day from its fever clinics—more than twice the total reported cases by onset (CDC) at the time when so many cases were flooding the fever clinics that they have to “expand the fever clinics in 04/01/2020”. This is consistent with the eyewitness report on dozens of times 🚑, at least 2 from Jiangxia (remember only 1 dot on the WHO map was from Jiangxia) rushing across the ShiPaiLing road leading to the “中部战区总医院” in 31/12/2019.' gab.com

@NestCommander - Kevin W. McCairn PhD

The only thing governing the probability for positivity of the environmental samples is “closest to the toilets” and “closest to the main entrance of the market”. archive.md/yyX0Z archive.md/iw1Pz archive.md/4rVph archive.md/DChUL Also here is a result on the raccoon dogs and the inability for the species to become infected in nature. archive.md/n9o0f All non-human mammals archive.md/7doR8 archive.md/0A24q at most landed on different sections of the ground and correlation fails upon entry to that “raccoon dog stall”. archive.md/Ttn5P archive.md/JSQvc Coincidence caused by pathological spatial distribution on the most uniquely found species in the stall closest to the toilets archive.md/gvHfw have high R^2–all landed on different sections of the ground and fails upon entry into the stall. archive.md/0A24q True causation remain positively correlated when looking at the positive samples or when you enter the site of the pathological spatial distribution. archive.md/csYBM Also, in order to test positive in Gao et al, a sample archive.md/CTP3i archive.md/ETjzS archive.md/BWZJL must be contacted by a sampler. 🥼👖👢=positive. archive.md/NeybM archive.md/2PM9Y archive.md/RirQ7 Must not be frequently handled by a vendor. 🥩🥬🍄🛁📻☎️📦🧺=negative. There is a reason why the theil-sen correlation, a quantifier of mutual information, show Homo Sapiens as max correlated wherever any species in the “susceptible mammals” category (wildlife and humans) show correlation at all.

@NestCommander - Kevin W. McCairn PhD

And of course, Polymerase stuttering is exclusive to Influenza and other negative ssRNA/DNA viruses (requires an “unzipping last” transcription mode which the nascent transcript is unbound from the template immediately after synthesis) and in fact, the S1-S2 is a cold spot of recombination because CoV template switching depends on TRS-B binding to Nsp7 and not by “stem loops”.

@lissnup - İsim Arıyor

too weak to drive it’s emergence. https://onlinelibrary.wiley.com/doi/10.1002/jmv.26478 In fact, the S1-S2 sequence is a RNA mutational coldspot in Coronaviruses, which means the same nucleotide can be conserved for sequences up to 6% divergence or more. These sites are endpoint sites of RNA editing, (12/26)

@NestCommander - Kevin W. McCairn PhD

Reverse transcriptases are critical in understanding the dynamics of SARS-CoV-2 genomic insert evolution. “How many billion hosts are needed for the GISAID sequences to appear? (2 billion) what kind of methodology they were sequenced with (because multiplex PCR+bad primer batch=additional sequences end up being inside the amplicons especially same supplier of primers used), and how many hosts a wildlife farm is able to harbor at maximum? (Less than 2 or other markets that the farms have to supply to because of the low animal counts in Huanan would be infected and have primary outbreaks) What happened to those “lineages”? (They disappeared upon the exhaustion of the specific primer batch they were sequenced from, and never continue circulation within sequences for more than ~2 weeks)” . It is something that they can not answer. no proline no VERO growth Destroyed in VOC evolution and no glycans to be seen https://gab.com/Flavinkins/posts/111398506038803573 The proline is required for efficient growth in VERO cell cultures and all live hosts despise it. With nearly a billion sequences available now all manner of sequencing error and biomaterial manufacture errors can happen. That is why none of the pandemic era inserts in covid lasted for more than the length of time which a batch of primers ordered from a company usually last in the testing and sequencing labs. If you look for dirt this big a base number and the high random and systematic error rates would guarantee something you will see. Also the “potentially real inserts” are all from within the SARS-CoV-2 genome. No CGG-CGG pair anywhere within the genomes of the closest relatives of SARS-CoV-2.

Flavinkins on Gab: 'Alpha, Delta, both have less VERO E6 than Wuhan/D…' Flavinkins on Gab: 'Alpha, Delta, both have less VERO E6 than Wuhan/D614G. Omicron, also. It is also the least VERO-growing lineage. http://virologyj.biomedcentral.com/articles/10.1186/s12985-022-01802-5 http://www.nature.com/articles/s41586-021-04266-9 http://www.sciencedirect.com/science/article/pii/S2589004221015595 This is NOT something you expect from a wildlife virus. They shouldn’t be adapted best to lab cell lines right out of the box. All adaptation trajectories (both from live mice, humans and the modified modern research cell lines) lead away from VERO E6 when an small carnivore/bat zoonosis scenario should simultaneously increase all primate tropism as the virus evolve in humans. It should raise, and not drop, VERO E6 tropism.' gab.com

@NestCommander - Kevin W. McCairn PhD

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7239183/ All 3 Lines of PO argument have been debunked. There is no evidence of O-linked glycans in the actual SARS-CoV-2 spike for cells that are relevant to real live hosts or immune systems. https://zenodo.org/record/6849652#.ZKUlyCV6slT And RmYn02 having 2AA shorter than normal not 3AA longer. vixra.org/abs/2010.0164 All RmYN02 “proves” is the motivated reasoning of the final PO public arguments. Even the authors themselves do not believe their arguments point strongly toward anything.

Deducing the N- and O-glycosylation profile of the spike protein of novel coronavirus SARS-CoV-2 The current emergence of the novel coronavirus pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) demands the development of new therapeutic strategies to prevent rapid progress of mortalities. The coronavirus spike (S) protein, ... ncbi.nlm.nih.gov
The Pan-SL-CoV/GD sequences may be from contamination. ABSTRACT Recently, There were much hype about an alleged SARS-like coronavirus being found in samples of Malayan pangolins (Manis Javanica) possessing nearly identical RBD to the SARS-CoV-2 coronavirus. Prominent journals cite the alleged discovery to claim that pangolins may be one of a possible intermediate host for the zoonotic transmission of SARS-CoV-2 to humans. Here, we report that all databases used to support such a claim, upon which metagenomic analysis was possible, contained unexpected reads and was in serious risk of contamination. Here we also report that the presence of unexpected reads are directly related to the presence of coronavirus reads. Finally, we deduced the actual causative agent of the death of the pangolins sampled in GuangDong 2019 where the claim of coronavirus detections was made. zenodo.org

@NestCommander - Kevin W. McCairn PhD

And of which all that existed were just viruses that were smeared out of the toilets and shed by researchers, and which nearly all positive samples are below step height, no positive samples are above waist height and that none of the highly touched locations are positive. Humans=shed in the toilets and feces are stuck all over the boots and suits and shoes and clothes of the samplers and vendors alike. Suit-stained walls doors and legs of desks (but not tops of tables), boot-kicked machines, cages, carts, scales and of course the ground itself which is the dominant sample type for positive samples. And suit-stained sample tubes where the swab is clean but the lip isn’t (causing PCR-/NGS+). In fact all animals that can be infected at all shed in their feces for SARS-CoV-2 RNA. Yes. SARS-CoV-2 have enteric tropism and shed RNA in feces for both animals and humans. You know that transfer contamination is the dominant if not the only mechanism for market environmental samples when there are also samples that are +ve in both PCR and NGS, but linked neither to human cases nor to wild animals. Even the presence of materials from different origin within the samples are consistent with transfer contamination with a pathway that first go through the toilets and then go through the W6 junction, getting SARS-CoV-2 on the former and wildlife material (on only a fraction of the boots) on the latter, independently. More samples with neither cases nor wildlife DNA are found south of the W6 junction than north of it, but such samples also exist north of the W6 junction. This is consistent with the virus being brought in from the entrance/toilets, contaminating stalls where there is also a focus to stalls with human cases. When boots stepped through the W6 junction, some of the boots also have wildlife DNA stuck to them, bringing it alongside when sites north of the W6 junctions were kicked or trampled. but not all of them were and there exist also incontrovertible proof of samples with neither human cases nor wildlife DNA found also here. Good and specific PCR primers, like Jan 01/Jan12 ORF1ab+N, and you should have PCR+ before NGS+. Bad and cross-reactive PCR primers like an ORF1ab only primer, and you are going to have PCR+ anytime you see material from the same family you are trying to test on (Embecoviruses cross reacted with their ORF1ab primers—and these animal CoVs are the only real grounded CoV consistent with samples of the expected age at sequencing found here in the specified time). However, PCR-/NGS+ is something that should never happen nomatter which primer pair you use (cross-reactive or specific) when your NGS result place clustered reads right beside the primer pair.

@NestCommander - Kevin W. McCairn PhD

Since C-C say you need to @stevenemassey use qPCR to properly get the viral counts, let’s see…… Q61/Q70=PCR-. (And located uncomfortably close to PCR+ samples rendering them prone to contamination on NGS.) Q37=PCR- AND orphan sample negative whole stall before and negative exact site after. And primers aligned over by NGS. All are false positive samples. All does not prove virus is there with that metric. The virus is in the human+ and animal-poor Q64/Q68/Q69. What they wanted you to believe: Aerosols are blocked by walls and can not spread from toilets and wildlife stalls. Reality: Activity of samplers and vendors alike, especially their shoes and boots and the gloves of the samplers, caused the contamination to be spread out from the toilets. What they wanted you to believe: there are additional PCR+ samples. Reality: these are a different kind of PCR than what Jan 01 and Jan 12 used. It lacked lacked the universally present N primer pair in the specific PCR primers (the Jan 01 and Jan 12 used specific ORF1ab and N primers in the same reaction to generate 1 single Ct value) which indicate it being an non-specific (surveillance primers in PREDICT target only the ORF1ab/RdRp region due to its conservation, and have degeneracy.) test that cross react with all members of the Coronaviridae family. Artifacts ensues, if not “no reads at all”. Neither PCR+/NGS- nor PCR-/NGS+ can be trusted as genuinely positive, due to the extreme proneness to contamination in the NGS pipeline and the probability of cross-reactivity in some PCR tests. archive.md/2PM9Y archive.md/RirQ7 archive.md/CTP3i archive.md/NeybM archive.md/ETjzS archive.md/BWZJL https://pdfhost.io/v/~IGA2bONb_closest_to_the_toilets https://pdfhost.io/v/dUbkceTFh_anticorrelation_is_not_an_artifact And the reason why the samples in the market follow the rule which a positive sample archive.md/CTP3i archive.md/ETjzS archive.md/BWZJL must be contacted by samplers.🥼👖👢=positive. archive.md/NeybM archive.md/2PM9Y archive.md/RirQ7 And not frequently handled by vendors.🥩🥬🍄🛁📻☎️📦🧺=negative; Is the same reason why you only get animal viruses but not SARS-CoV-2 legitimate reads past 12/01/2020. https://assets.researchsquare.com/files/rs-885194/v1/78e0e6ce-4a76-48de-9f5c-76bab452bbe6.pdf?c=1665607885 Not found inside actual animal tissues because the animals are not infected, isolated SARS-CoV-2 virions are exceptionally sensitive toward destruction by RNAse 7 found on human skin, and they all got completely destroyed before the samples can even reach the testing lab if it contained material from a highly touched surface. Surfaces that see any hand contact at all in a hospital room just vaporized when the patient leaves, leaving only the floor behind which survive even terminal clean. Likewise, the skin surfaces of caretakers are free of RNA even when their stool become positive. Objects that always have either walking patient or HCWs touch like door handles keyboards or toilet seats have low prevalence even when the patient is inside the room, and objects which for a large fraction is touched only by gloved caregivers (bed rails) and objects that are strictly not allowed touching without a glove (ventilator buttons) when a patient is active, have the most SARS-CoV-2 RNA on them, where ventilator buttons which have 0% skin contact have much greater prevalence than bed rails which skin contact is absent in ICUs (where the positives came from) but present in normal wards. Similarly, heavily handled surfaces like old banknotes Are found to completely destroy SARS-CoV-2 RNA as little as 10 hours after deposition/material mixing to the same environment. Less handling and slightly longer life. Contrast clean surfaces like fresh PPE which the RNA remain stable for a month.

@NestCommander - Kevin W. McCairn PhD

https://assets.researchsquare.com/files/rs-885194/v1/78e0e6ce-4a76-48de-9f5c-76bab452bbe6.pdf?c=1665607885 in fact the nature of SARS-CoV-2 here being superfluous contamination that have an origin in human secretions and cell culture supernatants brought into the stalls by the samplers and on boots, shoes and suits is obvious here. Independent sampling indicate that only the animal viruses highly correlated with the animals were left in February. The SARS-CoV-2? Superfluous, in human metabolic products and secretions, and not in butchered tissues. They are cleaned off and degraded efficiently. The animal native viruses are inside the tissues and are highly persistent—class of contaminant type different and SARS-CoV-2 is in the class that is inconsistent with the behavior when pitched against cleaning than animal CoVs (which unlike SARS-CoV-2, the animal viruses including CoVs persisted in tissue fragments of animals generated from butchering and animals banging against cages for more than one month, whereas all the “SARS-CoV-2” after 12/01/2020 are either amplicon artifacts, PREDICT primer false positivities (no reads at all), or evidently freshly prepared cell cultures which even the fragile mitochondrial transcripts of the Homo Sapiens and intracellular viral transcripts of the SARS-CoV-2 have been preserved (they decay within a day after loss of cell viability https://www.ncbi.nlm.nih.gov/pmc/articles/PMC369693/) and where no non-human mammals at all were present. (Clearly a recent cell culture added into these “storehouse” samples). https://archive.md/13bdP archive.md/FskYn archive.md/gvHfw archive.md/4cCHG archive.md/csYBM archive.md/rj1pV archive.md/LJzSO archive.md/4cCHG boot on surface = NGS+/PCR+, suit on sample tube = PCR-/NGS+ as it indicate contamination occurred after PCR and before NGS especially when with alignment over the ORF1ab primer, and that the location then got a total negativity same stall before and same site afterward. Closer to the toilets, more likely of direct stall entry after market entry by the sampler, more samples become contaminated. Earlier the time of first sampling, the more virus in the contamination source at the entrance with less disturbance, and more virus is found in a sample that is taken from such a stall. And yes. One of the earliest unknown activity done by the WCDC including “taking environmental samples” and “cleaning” the market overnight in 31/12/2019. While the archive.md/iw1Pz animal samples have been disclosed in 01/2020 and all negative, the focus on early cases stalls in this run brought in the virus into the “live virus isolated” stalls that would be sampled in 01/01/2020.

Synthesis and turnover of mitochondrial ribonucleic acid in HeLa cells: the mature ribosomal and messenger ribonucleic acid species are metabolically unstable. The synthesis rates and half-lives of the individual mitochondrial ribosomal ribonucleic acid (RNA) and polyadenylic acid-containing RNA species in HeLa cells have been determined by analyzing their kinetics of labeling with [5-3H]-uridine and the changes ... ncbi.nlm.nih.gov

@NestCommander - Kevin W. McCairn PhD

Trickery like using unauthorized sprayers to either put virus onto the stall (guess why all positive samples are below waist height? Yes. Sprayers are all aimed down by design and droppers can only be pointed downward when being used) immediately before sampling (resulting in NGS datasets containing transcriptomes of cells and virus that were far too fresh to be as old as a month and a half since the market is closed, if they have waited 11 days between putting the virus in and taking the samples that would have been able to weasel their way through) or that you put some additional Amplicons from another experiment-in-validation (keep in mind that the ORF1ab only primer pair as used in the “ORF1ab” PCR is distinct from the “ORF1ab/N” primer pair used before, as PCR kits of single Ct values are based on pre-mixed primer/probes that can not be separated for independent use, let alone “running out for just one primer”.) prior to running PCR in a sample (you get what you put in at NGS—amplicon only and other things that can react to the degenerate ‘ORF1ab’ primers but is not SARS-CoV-2 that are generated in your source experiment, but not legitimate SARS-CoV-2 reads) simply result in artifacts that clearly show evidence of sample manipulation within the resulting “data”. As the WCDC itself was also tasked to generate proof of whatever the most popular theory on zoonosis in that time, at first they attempted to just put human SARS-CoV-2 cultures into the wildlife stalls before a species is specified, yielding samples that correlated only With Homo Sapiens in a consistent manner or with significant mutual information. Then the primary suspect becomes “snakes” in that now debunked-by-ACE2 “codon usage” paper, https://onlinelibrary.wiley.com/doi/abs/10.1002/jmv.25682 And they were tasked to find a way to either remove or justify the human reads inevitably introduced with the virus. First they used their PREDICT primer pair (+SYBR green), which cross reacted but did not yield NGS SARS-CoV-2 reads. Then they tried putting one of their early WIP RdRp+multiple site amplification (one of the many different trials for suitable primer locations on the viral genome for distinguishing amplification prior to final probe design, already very close to their current N/E sites) experiments into the newly made “snake stall” samples, which supposedly exclude Human (some still snuck in alongside). The result are reads that are obvious artifacts that can not convince even untrained critics. Attempting to then justify the human reads if they can’t remove it, they went to the snake stall again and now took cultured virus straight from their incubators, mix it with “snake” meat samples impounded from earlier samplings (contained mixture of meats often sold as snake, but no mammals of any kind at all) thoroughly, put the result into the “storehouse” and immediately took swabs. Because of the immediacy of the action, the results are far too fresh by transcriptome to have been possibly deposited at or before 01/01/2020. (RdRp-based “ORF1ab primers” especially if there are other tests that are in development such as the “RdRp/N/E” tests are notorious for their cross-reactivity especially before the conditions are fully dialed in—the intermediate stage “ORF1ab only” primer https://assets.researchsquare.com/files/rs-885194/v1/78e0e6ce-4a76-48de-9f5c-76bab452bbe6.pdf?c=1665607885 also happens to be the primer used in the time when the animal CoVs are being identified in the market but not SARS-CoV-2 in their amplicons. The RdRp/N/E tests later generates 3 separate Ct values, indicating each test used 3 reactions likely validated separately, even on different sets of samples—https://www.sigmaaldrich.com/AU/en/technical-documents/protocol/genomics/qpcr/sybr-green-qpcr even primer dimers would react and that the command “take one amplified experiment each and put it into the environmental samples before PCR” would lead to legitimate read-free “ghost” reactivity, especially for experiments and primers that were still in early evaluation at that time. Same for contamination out of it.)

Universal SYBR Green qPCR Protocol Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions sigmaaldrich.com

@NestCommander - Kevin W. McCairn PhD

(Note that samplers if they just came out of a lab doing specific PCR test development, viral amplicons and other complicated and not-all-in-1 experiments that involved the amplification of SARS-CoV-2 and other viruses, these can also drive contamination of the surfaces by the amplicons without deliberacy. Same if the workers have just attended to cell cultures in the lab—February Wuhan is actually among the time when PPE supplies especially the isolation suits and gloves/boot covers are in such a short supply that many disease control and hospital workers could only use one suit for an entire day—without being able to replace it even between tasks. You can expect that alongside sampling that got focused because of the snake theory, such compromising action to pull clearly artefactual intermediate-in-lab material out and into the environmental samples especially taken at the same day. Plus PREDICT primer cross-reactivity which this particular pair was used from 0127 to 0219.)

@NestCommander - Kevin W. McCairn PhD

@stevenemassey No. There is really no SARS-CoV-2 RBD in Wuhan.

@NestCommander - Kevin W. McCairn PhD

“Perfect synergy” that no live hosts wanted to keep. Proline: destroyed in all VOCs. Did not stop or prevent further animal infections with all the ones found infected before still commonly infected (no species were sold in Huanan). QTQTNS: became QTQTKS. Did not stop animal infections either, expands tropism in stead. The only things they are good in are in VERO/HAE and CaLu-3 cells.

@NestCommander - Kevin W. McCairn PhD

@stevenemassey It looks like raccoon dogs and civets are both entirely uninfected. Oops.

@RetractionWatch - Retraction Watch

“Criticism of statistical analysis on the origin of Corona.” https://www.faz.net/aktuell/wissen/medizin-ernaehrung/wo-der-corona-ursprung-wirklich-lag-fruehere-analysen-in-kritik-geraten-19476294.html

Wo der Corona-Ursprung wirklich lag: Frühere Analysen in Kritik geraten Vor zwei Jahren schien die Frage, wo das Corona-Virus seinen Ursprung hatte, weitgehend geklärt. Statistiker kritisieren nun frühere... faz.net

@NestCommander - Kevin W. McCairn PhD

@stevenemassey In fact, the entire “market centered” Chinese “early cases” data is tampered with and fraudulent.

@NestCommander - Kevin W. McCairn PhD

@stevenemassey HKU3 and ZC45 are not SARS1 or SARS2, nor were the “hubei civets” with Spike proteins nested well inside the Beijing strains of SARS1 a valid progenitor—it is a spillback infection. Nothing more.

@NestCommander - Kevin W. McCairn PhD

@stevenemassey

@NestCommander - Kevin W. McCairn PhD

@stevenemassey And unfortunately, the “Hubei civets” are simply just spillbacks. And also, once again, ZC45r-CoVs=/=SC2r-CoVs.

@NestCommander - Kevin W. McCairn PhD

And even worse, the expected systematic social and populational biases from a survey of residences was too ignored within the China WHO “dataset”. An “meter precise” centering toward the market is exceptionally improbable from residences because they are too unevenly distributed around the market, biased populationally too toward the Wuhan CBD, meaning that even those infected near or are gathered from nearby the market should not have created an perfect centering of their KDE in a residence-free location of within 50m radius exactly above the market itself. To have this level of centering mean tampering with the “data” further, which leads to the majority of the “bullseye” cluster are not found on residential areas and that many of the “case residences” lands on water, which residences can not exist on without being washed away. Guess again why China never allowed any of the early case line list data or raw data of any kind to anyone? (And guess why they never dared to say where the first case they ever admitted or any cases at all lived at any later)?

@NestCommander - Kevin W. McCairn PhD

Not even rumors indicated any person at all in the wildlife industry in China being sick or getting infected, not even rumors indicated direct participation with the wildlife trade (purchasing, vending, dealing, transporting, farming, butchering, cooking or eating) by any of the known official or unofficial early cases. The only ever results from these wildlife trade participants indicate perfect condition of health and no evidence of infection at all among the customers or neighbors of any of them. Not even the market cases themselves—none of them reported direct participation of the wildlife trade. And unfortunately, The only thing governing the probability for positivity of the environmental samples is “closest to the toilets” and “closest to the main entrance of the market”. In fact, the samples in the market follows the rule which a positive sample archive.md/CTP3i archive.md/ETjzS archive.md/BWZJL must be contacted by samplers.🥼👖👢=positive. archive.md/NeybM archive.md/2PM9Y archive.md/RirQ7 And not frequently handled by vendors.🥩🥬🍄🛁📻☎️📦🧺=negative. archive.md/LJzSO archive.md/4cCHG boot on surface = NGS+/PCR+, suit on sample tube = PCR-/NGS+ as it indicate contamination occurred after PCR and before NGS with alignment over the ORF1ab primer. Closer to the toilets, more likely of direct stall entry after market entry by the sampler, more samples become contaminated. Earlier the time of first sampling, the more virus in the contamination source at the entrance with less disturbance, and more virus is found in a sample that is taken from such a stall. And yes. One of the earliest unknown activity done by the WCDC including “taking environmental samples” and “cleaning” the market overnight in 31/12/2019. While the archive.md/iw1Pz animal samples have been disclosed in 01/2020 and all negative, the focus on early cases stalls in this run brought in the virus into the “live virus isolated” stalls that would be sampled in 01/01/2020. https://wwwnc.cdc.gov/eid/article/10/6/03-0852_article On the contrast, 5 independent cases with close contact to the avenues of wildlife trade for SARS-CoV-1 have happened in 5 cities in 4 in Guangdong and 1 in Guangxi, over the same 2-months timeframe. Two of them were market workers on two independent markets which civets were sold, three of them were direct participants of the wildlife trade: two of them were civet butchers, and one a driver for wildlife dealers. All of these cases have yielded continued transmission from them. In the contrast, 0 of the early cases for SARS-CoV-2 worked in or have a history of direct participation with the wildlife industry. archive.md/yyX0Z archive.md/iw1Pz archive.md/4rVph archive.md/DChUL Exactly 0 raccoon dogs or any of the so-called “susceptible species” were found infected anywhere in the world, not even by a relative of SARS-CoV-2.

Epidemiologic Clues to SARS Origin in China SARS Origin in China wwwnc.cdc.gov

@NestCommander - Kevin W. McCairn PhD

Again, asking: why they have to ban the Wuhan P4 lab from mentioning in 31/12/2019, before any theories can even be made? (Also notice that the first market case neither worked with the wildlife trade, nor did she even play mahjong like the later cases did when the linked cases were first gathered by a citywide command for them over 30-31/12/2019. Once again indicating that the market outbreak was likely first brought in from the outside, then superspread at the toilets and mahjong rooms later.)

@NestCommander - Kevin W. McCairn PhD

Why Daszak’s name ended up in the GenBank file of a—Laotian CoV, which they claim to have never sampled especially alongside Shi as well? “ID’ing 50+ novel strains”. There is Inconsistency piled upon inconsistency in Chinese publications as well as “data”. And he just held onto his secrets for 9.5 hours and also, notably, failed to produce a counterargument, after 9 and half hours. The ODNI broke the law and their “report” is full of elementary mistakes. More hidden experiments.

@NestCommander - Kevin W. McCairn PhD

Also, the only bat cave that was banned from public access—were Mojiang and Shitou. It happens only at and serves only to obscure the actual inventory under their control in the sampling and testing sites of the WIV. And they Did not “Stop all bat sampling”. There is clearly evidence that they are perfectly capable of hiding their work, none of the claimed official audits to the lab was ever published or even disseminated among the authorities in China, And the reason why they used GISAID here, is that https://archive.md/0aHWr https://archive.md/Myt4u there is no proper versioning or custody of “data” on GISAID. https://archive.md/52DyQ https://archive.md/B0xlW @DiLiMengYAN1 And no trails that can be FOIA’ed and bust their “data” like on NCBI. And no possibility that inconvenient lab-incriminating data can be leaked or FOIA’ed like csabai et al. Still no official response from the CCP.

@NestCommander - Kevin W. McCairn PhD

“They would close the lab and raze it to the ground”: That is admission of guilt, not cover-up. What they actually did: forged the environmental samples by spraying the wildlife stalls with the virus in Jan 02. Erased the superspreading site of the toilets in Feb 13. Refused to swab anywhere in Wuhan outside the market or its immediate vicinities, not even other corners of the market. Shoved all the cases with original residence in Wuchang into the market. No government-funded lab have been shut down after a leak, even when outbreak and outrage ensued. The interest in keeping the labs active and operational is that of national security, keeping the biodefense industry stable and up-to-date. They are never shut down, only repaired usually with as little disruption or outside visibility as possible. (WIV had an 2 year batCoV research publication hiatus, despite attempt to keep mitigation efforts secret). No civilian interests can interrupt the operation of these labs. https://www.thefreelibrary.com/Farmers+demand+a+Pirbright+shutdown%3b+%27A+private+company+would+have...-a0168453941 Not shutting down after leak is also one of the decisions that well, even democracies, does. and Yes. Pirbright is back at FMDV again after the leak. “Does this look like what a lab would do after leak causing hundreds of millions of pounds of damage”? https://en.wikipedia.org/wiki/2007_United_Kingdom_foot-and-mouth_outbreak Fact: Pirbright was not shut down after FMDV leak infecting 4 farms nearby. They repaired their drain pipes and continued operation, not even interfering academic publication patterns. Ironically, cow farms were shut down and beef trade was closed during the outbreak. This resulted in an epidemic lasting 5 months in cows that lead to at least two major cullings and severe disruption to the livestock trade from the U.K. That is, the reaction look like what they claim a zoonosis would look like, not what they declare what the WIV would do when such a shut-down would certainly directly admit guilt and spell doom to both the institute and its operators. https://www.theaustralian.com.au/science/beijing-lab-mishap-infected-scientist-with-covid19/news-story/9b0cb0ed84df21d25da11b698be3611a Fact 2: there is no shut-down reported at all in the IVDC either after the 2004 leak of SARS or the 2020 leak of Covid. Not even a burp of interruption. Fact 3: the WIV went on hiatus to the bat CoV isolation tests over 2020-2023. When the sverdlovsk anthrax leak happened, they blamed the animal farms and markets nearby and did not officially shut down the facility. The construction of another anthrax facility nearby was considered potential indication of a shut-down, which is on par with the WIV hiatus. After a timescale similar to the WIV hiatus, the new facility was opened for inspection which no anthrax was found, meaning that they fixed sverdlovsk and went on, just like the WIV (chen WEI……). In facts, there have not been a single record of an lab leak or LAI in a research facility that resulted in the (especially permanent, as what they claimed would happen) shut down of the facility (despite hundreds of known incidents in record), even when significant epidemic have occurred from the event. (Ebola21, FMDV07, H1N177, Anthrax82 which no official shutdown was known).

Farmers demand a Pirbright shutdown; 'A private company would have closed'. - Free Online Library Free Online Library: Farmers demand a Pirbright shutdown; 'A private company would have closed'.(News) by "The Journal (Newcastle, England)"; Business Business, international News, opinion and commentary Agricultural industry thefreelibrary.com
2007 united kingdom foot-and-mouth outbreak - Wikipedia en.wikipedia.org

@NestCommander - Kevin W. McCairn PhD

there is a long history of the WIV lying to the point of base rate neglect when being asked anything about potential LAI. The “dinner of staff” too, where they neglected the base rate which is Wuhan medical institutions are already in panic and the general public is already taking precaution, as h2h is announced in 15-16/01/2020 to the point that even the invited international collaborator have hinted Shi to wash hands, that she unexpectedly did not given her expertise and knowledge on the public info about SARS-CoV-2 in general Wuhan public in this time. She pretended to not know the need to take precautions when she was expected to do so, just like when she sabotaged the test to make 67 general 2021 Wuhan public serological samples test all negative when there should be positives given the seroprevalence in Wuhan at that time. researchgate.net/publication/35… It is just as impossible To have 67 community members to test all negative in Wuhan in 01/2021 as to have 593 people to test all negative with any sensitive test available in April-June 2023. gab.com/Flavinkins/pos… And this same behavior of issuing a test that will not turn positive on a human also happened to the mojiang miners. Where their own early serological test results were contradicted. archive.md/Pc6gp archive.md/zUD1F And ben HU lied about working with live virus which are so easy to debunk just by a simple google search. His own grant notice required live virus work in 2019.

@NestCommander - Kevin W. McCairn PhD

Importantly, none of the species in that “stall” were truly susceptible and 0 individuals of any of the species have been observed with an infection with a relative of SARS-CoV-2 in the wild. “Susceptible species” is nothing but a myth and this reflect well by the fact that None of the “susceptible species” were actually in positive correlation at all with the SARS-CoV-2 reads once you enter that “stall”—it is confounded by the toilets and all it had in it is sampler contamination, just like the outside of stall W4-26-28. archive.md/gvHfw archive.md/csYBM archive.md/vlAgp archive.md/DChUL archive.md/4rVph archive.md/yyX0Z Despite surveillance in Europe and Japan, there were zero evidence of natural infections in a raccoon dog anywhere in the world. archive.md/iw1Pz All upstream suppliers are traced and were negative. In fact, none of the “susceptible species” have evidence of even a single individual being infected by SARS-CoV-2 or its relatives anywhere in the world. archive.md/VNr75 archive.md/rj1pV All 3 lineage A samples have direct link to the WCDC. The stall of A20 have owners that Wore slippers and handle fish with bare hands. They don’t wear gloves and shoe covers can’t be worn over slippers. This sample is contamination caused by the WCDC itself and there is no case from the market that is lineage A. This same infected sampler, that the WCDC would even admit, would then go on sampling the wildlife stalls in Jan 12 and rub his contaminants all over the surfaces on the closest stall to the toilets and sample tubes. This then drive confirmation bias on snakes which lead to more sampling and more contamination, all contained nothing but artifacts and never on the original site of the contaminated sample tube again, eventually leading to samples with only human DNA and no other mammals at all inside. Zero attempts have been made to gather evidence at the WIV. https://pdfhost.io/v/kZ1ilPCFa_The_real_problem_is_that_there_was_literally_zero_attempts_at_gathering_any_evidence_at_the_WIV Or anywhere else. https://pdfhost.io/v/dUbkceTFh_anticorrelation_is_not_an_artifact And the anticorrelation with raccoon dogs aren’t “an artifact”. The “all samples” correlations are artifacts of pathological spatial distribution from confounding factors that extracted the species found in the least number of overall stalls that happened to include the stall closest to the toilets and entrance into the market during the sampling run. https://pdfhost.io/v/~IGA2bONb_closest_to_the_toilets This mean that all correlations other than humans failed when the analysis is to be done in a way which the pathological spatial distribution is mitigated in any way. archive.md/MtkL3 All species other than humans at most landed on entirely different sections of the ground and set of items than the SARS-CoV-2 reads, and only humans are found on the same sections of ground and set of items in the form of sampler-linked contaminants. This is evident when the correlation is performed with only samples where SARS-CoV-2 is found, which effectively queries “which species shed the SARS-CoV-2 sequences in samples where it was found” as opposed to “which set of species most uniquely represents the spatial features of the single stall closest to the toilets”. Hedgehogs have proven non-susceptible ACE2. Oh. On the entire “early cases map”: @CharlesRixey The entirety of that “map” was created using concocted and fake “data” spoon-fed by China that wasn’t available to the public even this date. archive.md/5sdkR archive.md/1pcCU archive.md/N0hib archive.is/Kyr1z archive.md/VXtu9

@NestCommander - Kevin W. McCairn PhD

@AntGDuarte And here is a hint: despite being found all over the stalls and were also the objects most handled by the human cases, even in stalls with human cases and where there were no wildlife DNA in the positive samples from the stall, no boxes or baskets in the market have tested positive. The same for cashiers, keyboards, monitors, water cups or any objects that are frequently handled by direct touch by a vendor on the surface where the swab will be taken from. They can never test positive because of the insanely high content of RNAse 7 and other defensive nucleases on human skin destroys SARS-CoV-2 virion RNA within the period which the samples are stored inside liquid medium before being tested (which is particularly effective when the RNA is found in loose virions or human metabolic secrations and not shielded inside solid animal tissues), and vaporizes archive.md/RirQ7 archive.md/CTP3i archive.md/NeybM archive.md/2PM9Y any virion-free RNA the instant it enters medium contaminated by it. The WCDC and the Hubei CDC stores all of the human samples and backups of research cultures of pathogenic microbes in Wuhan, as this is their legally delegated duty (the “各级疾控部门” are termed “保藏机构” for “病原性微生物” under Chinese law governing the use of cultures and samples of human and animal pathogenic microbial samples, and samples that were suspected to have the possibility of containing such microorganisms. These are also the only locations which first round samples arriving in Wuhan are allowed to go for pre-screening prior to entry into the other labs in Wuhan, “检测机构”. ) and that labs in China are not allowed to store such cultures except several select state key laboratories. Since 2014, the only EID surveillance target in Wuhan is the HSM which all other sites are kept blind so that they can blame Huanan in case the research labs suffer an accident. Almost the soon as experimentation begun in the WCDC at the first detection of an infection from that program (Chen/WIV), the prior culture samples that was identified to match (via preliminary testing, including RdRp and antigens which are targeted by the Military test kits used in Wuhan) ended up causing an employee infection. Creating all 3 lineage A cases afterward. The employee infection would end up being detected because the CDC have to use a kit that work on real patients unlike the WIV, and got whistleblown into the WHO report under the pretext of an again never-specified “family cluster transmission”. So bad that you can’t actually compare Serological tests that were conducted between distinct times and groups of people for the test itself because doing so violated the statistical homogeny criterion for test efficacy evaluation—the same as comparing 🍎 with 🍊.

@NestCommander - Kevin W. McCairn PhD

Reality 1: many different supply chain exist from Yunnan to Guangdong and Hubei is just infected in SARS1 by human cases. Reality 2: The actual count for animals farmed in China vs sold in Wuhan likewise indicate that Wuhan sell only a negligible fraction of all animal sales in China Especially when compared to Guangdong.

@NestCommander - Kevin W. McCairn PhD

https://www.nytimes.com/2021/02/12/world/asia/china-world-health-organization-coronavirus.html They systematically moved more than 3000 cases from the lab to the market and gave “cases data” that they wanted to push for market as first outbreak site to distance from the labs. https://archive.md/rYvu3 https://archive.md/UFrSv https://archive.md/nevZy https://www.researchgate.net/publication/370635299_Greater_than_the_Sum_of_its_Parts_-_Aggregated_Wuhan_COVID-19_case_data_points_to_the_wrong_side_of_the_Yangtze_River_-_Rixey_-_20230509 Such an result of having unlinked cases closer to the market than linked cases is not expected even under the null hypothesis of market origin, which we should see unlinked cases secondary to and cluster around the linked cases, and not the market itself. https://www.researchgate.net/publication/370635299_Greater_than_the_Sum_of_its_Parts_-_Aggregated_Wuhan_COVID-19_case_data_points_to_the_wrong_side_of_the_Yangtze_River_-_Rixey_-_20230509 Not only there were an complete absence of verifiability in Chinese cases, there is direct non-circumstantial evidence that they moved up to 3000 cases from Wuchang to Huanan. In fact, it is totally not normal to have unlinked cases closer to the market than linked cases—the only way this can happen is with ascertainment bias. Only near the market gets ascertained if not directly linked to it. Base rate neglect. They did the exact same thing when claiming that all 67 “pre-Huanan checkable cases” were “serologically negative”. Again, the social media associated here say “before Jan 18, 2020”. Included all Dec cases. https://www.mdpi.com/2220-9964/9/6/402 Before they begun enforcing their claim of “100/174 centered around the market” and starting to tamper with data to make the claim, https://ghrp.biomedcentral.com/articles/10.1186/s41256-021-00200-8 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149375/ 135/92 and 115/82 cases already got into in early peer-reviewed papers that went missing in the WHO report. Past media reports archive.md/Ea0Kw archive.md/1x658 also contradict WHO in key early cases’ residences, including the earliest case they admit in the WHO report. archive.md/5sdkR archive.md/1pcCU archive.md/N0hib archive.md/VXtu9 archive.is/Kyr1z https://archive.org/details/mace-e-pai-covid-19-analysis-redacted/page/8/mode/1up And you know that they hate this information when it was censored. The MACE-EPAI document here is not searchable on google. Up to one third of all cases were either removed completely or moved toward the market in the “dataset”. archive.md/zUD1F archive.md/Pc6gp https://archive.is/p3K3Z Including the very first case they ever admitted officially. And outright removed 4 times more cases than official. Unlinked cases supposedly secondary to linked cases should cluster around them, not the market itself. archive.md/GvRcD archive.md/ZgVzp Wuhan authorities after that archive.md/OIGPz 2014 incident now targeted only the Huanan market when looking for EID outbreaks—and nowhere else. archive.md/1x658 They tampered with the early cases data archive.md/Ea0Kw To make it look like it “started at the market” when in reality the first case they ever admitted lived right next to the WIV BSL-4. archive.md/5sdkR severe discrepancy happening December 2019 and January 2020 indicate tampering with case counts. archive.md/1pcCU This is indicative of catastrophic ascertainment bias was going on. None of China’s “early cases” dataset is credible. https://archive.md/ET1GA https://archive.md/Ea0Kw https://archive.md/1x658 The tampering of early case residence data is systematic and extensive. It is the reason why they refused to provide this data in any detail at all.

On W.H.O. Trip, China Refused to Hand Over Important Data (Published 2021) The information could be key to determining how and when the outbreak started, and to learning how to prevent future pandemics. nytimes.com
ResearchGate - Temporarily Unavailable researchgate.net
ResearchGate - Temporarily Unavailable researchgate.net
Exploring Urban Spatial Features of COVID-19 Transmission in Wuhan Based on Social Media Data During the early stage of the COVID-19 outbreak in Wuhan, there was a short run of medical resources, and Sina Weibo, a social media platform in China, built a channel for novel coronavirus pneumonia patients to seek help. Based on the geo-tagging Sina Weibo data from February 3rd to 12th, 2020, this paper analyzes the spatiotemporal distribution of COVID-19 cases in the main urban area of Wuhan and explores the urban spatial features of COVID-19 transmission in Wuhan. The results show that the elderly population accounts for more than half of the total number of Weibo help seekers, and a close correlation between them has also been found in terms of spatial distribution features, which confirms that the elderly population is the group of high-risk and high-prevalence in the COVID-19 outbreak, needing more attention of public health and epidemic prevention policies. On the other hand, the early transmission of COVID-19 in Wuhan could be divide into three phrases: Scattered infection, community spread, and full-scale outbreak. This paper can help to understand the spatial transmission of COVID-19 in Wuhan, so as to propose an effective public health preventive strategy for urban space optimization. mdpi.com
The comparison of epidemiological characteristics between confirmed and clinically diagnosed cases with COVID-19 during the early epidemic in Wuhan, China - Global Health Research and Policy To put COVID-19 patients into hospital timely, the clinical diagnosis had been implemented in Wuhan in the early epidemic. Here we compared the epidemiological characteristics of laboratory-confirmed and clinically diagnosed cases with COVID-19 in Wuhan. Demographics, case severity and outcomes of 29,886 confirmed cases and 21,960 clinically diagnosed cases reported between December 2019 and February 24, 2020, were compared. The risk factors were estimated, and the effective reproduction number (Rt) of SARS-CoV-2 was also calculated. The age and occupation distribution of confirmed cases and clinically diagnosed cases were consistent, and their sex ratio were 1.0 and 0.9, respectively. The epidemic curve of clinical diagnosis cases was similar to that of confirmed cases, and the city centers had more cumulative cases and higher incidence density than suburbs in both of two groups. The proportion of severe and critical cases (21.5 % vs. 14.0 %, P < 0.0001) and case fatality rates (5.2 % vs. 1.2 %, P < 0.0001) of confirmed cases were all higher than those of clinically diagnosed cases. Risk factors for death we observed in both of two groups were older age, male, severe or critical cases. Rt showed the same trend in two groups, it dropped below 1.0 on February 6 among confirmed cases, and February 8 among clinically diagnosed cases. The demographic characteristics and spatiotemporal distributions of confirmed and clinically diagnosed cases are roughly similar, but the disease severity and clinical outcome of clinically diagnosed cases are better than those of confirmed cases. In cases when detection kits are insufficient during the early epidemic, the implementation of clinical diagnosis is necessary and effective. ghrp.biomedcentral.com
Association of Public Health Interventions With the Epidemiology of the COVID-19 Outbreak in Wuhan, China Was there an association of public health interventions with improved control of the COVID-19 outbreak in Wuhan, China?In this cohort study that included 32 583 patients with laboratory-confirmed COVID-19 in Wuhan from December 8, 2019, through ... ncbi.nlm.nih.gov
MACE E PAI COVID 19 ANALYSIS Redacted : Free Download, Borrow, and Streaming : Internet Archive MACE E-PAI COVID-19 ANALYSIS archive.org

@NestCommander - Kevin W. McCairn PhD

Not only did The first every case they admitted live in Shidong right next to the BSL-4, and were moved toward the market in the WHO report in contradiction to all known media coverage, https://gab.com/Flavinkins/posts/109256201942085712 the entirety of Wuchang district was wiped clean for every single WHO case that have onset before 27/12/2019–with up to 3000 cases moved to the market this way over the entire Wuhan outbreak. https://archive.md/1x658 and for central Wuchang near the labs and the densest inhabited regions inside the district, all cases were moved away in the WHO map. Unfortunately Rasmussen's work on the origins question rests heavily on what David Relman described as "hopelessly impoverished" early case data. https://www.washingtonpost.com/national-security/2023/02/27/little-known-scientific-team-behind-new-assessment-covid-19-origins/ https://www.washingtonpost.com/opinions/2022/11/17/covid-early-cases-wuhan-china-mystery/ https://archive.md/ke1lp https://archive.md/RaYPC David Fisman: I think the most interesting thing this fellow says is that there are clearly tens of thousands of cases...That implies a much earlier introduction than would have occurred with a seafood market outbreak..." Also, Chen is not the only person infected in Shidong/Jiangxia and central Wuchang. Most were censored and only one of the two ambulances arriving in 31/12/2019 have been registered as a dot—likely because the origin wasn’t inside the Shidong prefecture/BSL-4 surroundings, and likely only because of being a close contact relative of Chen (contacting an known case). https://gab.com/Flavinkins/posts/109256201942085712 All dots they moved this way (up to 1/3 of all cases) was sent to Jianghan, https://archive.md/p3K3Z https://www.researchgate.net/publication/370635299_Greater_than_the_Sum_of_its_Parts_-_Aggregated_Wuhan_COVID-19_case_data_points_to_the_wrong_side_of_the_Yangtze_River_-_Rixey_-_20230509 especially to the immediate surroundings of the market, to scapegoat it and end up causing the “unlinked cases” cluster to be closer to the market than the “linked cases” cluster, despite supposedly the linked cases should be the only source of initial human to human transmission seeding and therefore the unlinked cases should cluster near the linked cases and not the market itself. This kind of improbable-under-null-hypothesis behavior is all over Chinese “data”. archive.md/VNr75 archive.md/rj1pV They attempted to spray their culture into the wildlife stalls, which ended up Making Homo Sapiens the only species that is found in every sample with a viral read in the market (note the absence of lineage reads in the wildlife stalls), and archive.md/LJzSO archive.md/4cCHG archive.md/13bdP all of the subsequent efforts at creating positive samples where the CCP specified them to do (“Blame snakes!” Is the official voice in 02/2020) just brought in artifacts first, and then when all of the mammals have degraded away, pure cultures of SARS-CoV-2 intracellular transcriptomes in human cellular transcriptomes. In addition to the heavy censorship of case ascertainment effectively mean you have to either live near the market or have a direct or indirect link to be diagnosed at all, moving all Wuchang case residence dots and sending them to Jianghan archive.md/1x658 archive.md/Ea0Kw also caused the “unlinked” dots to cluster closer the the market than the “linked” dots—something that can not happen without data manipulation on a massive scale. https://archive.md/ET1GA Unlinked cases are supposed to be seeded only by the linked cases if they didn’t visit Huanan under the market origin assumption. They are supposed to cluster near the linked cases and NOT the market itself. The CCP failed in this elementary logical analysis and resulted in a “dataset” that is too perfect to be possibly real. https://gab.com/Flavinkins/posts/108830214433800007

Opinion | Wuhan’s early covid cases are a mystery. What is China hiding? The story of how the pandemic got started — and turned into a global catastrophe — remains a black box. It should not be. washingtonpost.com
Flavinkins on Gab: 'https://gab.com/Flavinkins/posts/1088805315972559…' Flavinkins on Gab: 'https://gab.com/Flavinkins/posts/108880531597255968 https://gab.com/Flavinkins/posts/109147956977669077 Also, remember accountant Chen? (Why his dot was moved to the WCH if he lives in Wuchang/Jiangxia?) it turned out that it was not only his dot that was not in the right place. Every dot within the 2km radius of the Wuchang railway station was moved or removed. This is within the area that is expected to have the infectious disease and respiratory cases ultimately serviced by the “中部战区总医院”. The hospital that sees large-scale respiratory case anomalies the first in Wuhan on official records, where the decision to “enter battle stations” on 01/01/2020 was made because of an “unexpected and fast-growing anomaly in the respiratory disease surveillance data” beginning at least as late as 31/12/2019. There were dots that were east of this area, and there were dots that were south of this area, in locations with lower population density compared to downtown Wuchang and further from the market. (2 ambulances from Jiangxia on 31/12/2019, but only 1 dot on the WHO map and he wasn’t accountant chen…… (likely ascertained by contacting a HSM case on public transport, “试行诊疗方案”) (accountant Chen got to the WCH in 27/12/2019)) Considering how cases that were admitted to the “中部战区总医院” weren’t directly reported except for WH01 in 14/01/2020, (Only 1 out of the 4 known sequenced cases here were directly reported. WH03 is reported after transfer to the Zhongnan hospital, one of the 2 initial market cases reported in the location. WH02 and WH04 were not in the NNDRS dataset and displayed as “unknown” in the WHO report) and how they then report seeing more fever cases in a single day than the entire CDC pre-04/01/2020 onset dataset (the point when they have to expand their fever clinics), it is quite likely that cases that initially broke out in Wuchang were muted by admission into a hospital that is placed under a command that doesn’t have to report on the NNDRS, and that any cases found in Downtown Wuchang had their “residential addresses” altered to place them as close to the Huanan market as possible and out of the Wuchang area. This would not be the first time when cases that came from an “inconvenient” location were hidden inside PLA-operated hospitals to prevent them from being counted. https://gab.com/Flavinkins/posts/109701931477090563 Why the WMHC rejected the WHO’s demand for line listings of the 174 “NNDRS cases” in annex E2? Also, one dot in Jiangxia is one of the two https://gab.com/Flavinkins/posts/109048819612838694 ambulances that were seen in 31/12/2019 from Jiangxia. Only one become a dot (central Jiangxia as opposed to the Shidong prefecture). It is possible that this is Chen’s relative that “visited a local market”, meaning that this is a case that is ascertained by contact with an early case, and saved from removal because of post-27/12/2019 onset. No dot at all is inside the borders of the WuChang district, even when dots begin showing up east of it in less populated places further from the market. This is clearly artefactual, indicating attempt at breaking up the cluster in Wuchang.' gab.com
ResearchGate - Temporarily Unavailable researchgate.net
Flavinkins on Gab: 'https://archive.ph/dmOXT https://gab.com/Flavinki…' Flavinkins on Gab: 'https://archive.ph/dmOXT https://gab.com/Flavinkins/posts/108747087048451126 (You can find exactly 32 yellow spots “unlinked cases” in the 25% KDE contour of the WHO unlinked data……) https://gab.com/Flavinkins/posts/108731797608502118 (note: may contain cases admitted to the Houhu ward of the Wuhan central hospital even if them having a later date of hospitalization)' gab.com

@NestCommander - Kevin W. McCairn PhD

@AntGDuarte

@NestCommander - Kevin W. McCairn PhD

@AntGDuarte

@Florin_Uncovers - Florin

So the fraudulent coder in Pekar et al. '22 @niemasd ran Samson et al. '24 data and CONFIRMED their Aug-early Oct '19 tMRCA result!😅 Then he did what @michaelworobey & @jepekar did: discarded inconvenient data not realizing you also need animal sequences to model a bottleneck!🤡

@NestCommander - Kevin W. McCairn PhD

@AntGDuarte

@NestCommander - Kevin W. McCairn PhD

CAS “pathogen host adaptation and immune intervention” is one of such major grants that they continued DEFUSE on.

@NestCommander - Kevin W. McCairn PhD

Once again, 1: RaTG13 is not viable. https://zenodo.org/record/5702700#.ZJ2KiyV6slT https://zenodo.org/record/5778318#.ZJ5hyCV6slT 2: the real issue is that 1. WIV lies about everything serological. None of their “tests” were positive when politics require it to be negative. And 2. The missing sequences of Latinne et al is where you find what the WIV was working on. 7 SARSr and 54 total CoVs were missing entirely.

Anomalies in BatCoV/RaTG13 sequencing and provenance To this date, the most critical piece of evidence on the purposed “natural origin” theory of SARS-CoV-2, was the sequence known as RaTG13, allegedly collected from a single fecal sample from Rhinolophus Affinis. Understanding the provenance of RaTG13 is critical on the ongoing debate of the Origins of SARS-CoV-2. However, this sample is allegedly “used up” and therefore can no longer be accessed nor sequenced independently [1], and the only available data was the 3 related Genbank accessions: MN996532.1, SRX7724752 and SRX8357956. We report these datasets possessed multiple significant anomalies, and the provenence of the promised claims of RaTG13 or it’s role in proving a “probable bat origin”[2] of SARS-CoV-2 can not be satisfied nor possibly be confirmed. zenodo.org
The seminal paper from the Wuhan Institute of Virology claiming SARS-CoV-2 probably originated in bats appears to contain a contrived specimen, an incomplete and inaccurate genomic assembly, and the signature of laboratory-derived synthetic biology The bat coronavirus RaTG13 was purportedly identified in a bat “fecal” specimen that is probably not feces, has significant unresolved method-dependent genome sequence errors and an incomplete assembly with significant gaps, and has an anomalous base substitution pattern that has never been seen in nature but is routinely used in codon-optimized synthetic genome constructions performed in the laboratory. zenodo.org

@EMM_386 - EMM386

Ever want to create a novel coronavirus like SARS-CoV-2? Let Ralph Baric show you how to link 6 cDNA pieces flanked by unique restriction endonuclease sites and a swapped RBD. Just like "tinker toys". As seen in DEFUSE. Try different things - maybe use BsmBI instead of BglI?

Video Transcript AI Summary
We created coronaviruses by assembling a synthetic bat genome with the SARS clone. The genome was split into 5 kilobyte pieces with unique restriction sites to allow directional assembly. Initially, the virus couldn't replicate due to an entry defect, so we replaced the receptor binding domain with one from the human epidemic strain. This modification resulted in a virus that replicated efficiently. The growth curve data supported this success.
Full Transcript
Speaker 0: We build coronaviruses using our molecular clone as shown here with the SARS clone shown in blue. The clone is broken into 6, 5 kilobytes about 5 kilobytes pieces. Each piece is flanked by bagel 1 restriction endonuclease sites. These are Class IIs restriction enzymes that recognize a palindromic sequence but cut and leave asymmetric ends. This allows since these ends are asymmetric, they actually will not concatemarize like classic sticky ends left by restriction enzymes, but rather they become directional. So if you end clone A with a bagel site that leaves 13 nucleotide overhang and the 5' end of the B fragment with the complementary 3 nucleotide overhang, they go together directly. You change different bagel sites at each piece. And this allows them to assemble up into 30 kilobytes chromosomes like tinker toys. Now the synthetic bat genome that we made using Blue Heron and Bio Basic basically is interchangeable with the urbani clone. The only real difference was that we broke the F fragment into 2 pieces so that we could play with the receptor binding domain easily if this thing didn't turn out to be infectious. And in fact, we made this clone, we built a full on cDNA, we drove transcripts, electropated that in the cells, and we can see evidence of replication by the synthesis subgenomic messenger RNA, but we couldn't culture the virus and we couldn't pass it from cell to cell. So clearly, there was probably a defect in entry. To solve that problem, we used literature data that has suggested that RBD domains of coronaviruses may be interchangeable between species. So we took the human the Yervani epidemic receptor binding domain, that's 2 10 amino acids in the lab, and dropped that into the bat genome backbone, producing a chimera with the receptor binding domain driven from the epidemic strain. Now when we built that clone, drove transcripts and electroporated that into cells, we got a virus that could replicate quite efficiently. This is just some growth curve data showing I think the black

@NestCommander - Kevin W. McCairn PhD

https://archive.md/OIGPz The “Shunde problem” or “why it managed to infect Wuhan and only Wuhan”—is a problem which all market zoonosis or wildlife farm theories require extremely improbable and hard explanation to answer. Unfortunately the actual sales of wild animals in 2019 contained metadata-supported images or videos only in Guangdong and Guangxi, and not Wuhan. All observations of virologists working at the market without a published sample taken at that date should automatically be considered extremely suspicious. The most likely reason is that They were dropping in samples in stead of taking them, leading to the observation that only human have a consistent positive correlation or any significant mutual information with SARS-CoV-2 there. The reason why China intentionally hid nearly half of their flow cells is because they could use the reads inside to tamper with the “wildlife stall data” to meet the demand of the zoonati when given in 11/03/2023. They used it to scramble the host counts in all their “negative samples” when the correlational edge with Homo Sapiens were found to persist despite they removing the 300nt+ non-viral contigs and leading to an inverse correlation between the residual mitochondrial singletons * SARS-CoV-2 and the leftover contigs of other mammals as they were shredded by the common 43nt nuclear reads inside all mammalian genomes. Even before that, to prevent the obvious and embarrassing conclusion of “the SARS-CoV-2 is most likely smeared out of the toilets by the samplers” when both Jan 01 and Jan 12 have the stall with most positive samples turned out to be the one that is closest to the toilets and where the samplers entered and existed and a national plan was made to sample the toilets and public activity rooms in response, Wuhan ordered the bleaching and destruction of the toilet area before a sample can be taken from it. In fact, the civilian side of the national disease control apparatus was not even allowed to see the Q* samples in person or sequence them independently—They were not even allowed to verify any of the “qPCR results” and not even an Ct value would be “reported from the lab” which sent in the “sequencing results for Q* samples” directly. Eventually Xi ordered all Covid-relevant Departments to follow the same operational instructions over the end of February to the beginning of March 2020, the point of which an agreement was finally struck that they would work together to fabricate a “dataset” for animal origins, first as the primary (rewards were handed out to “find the animal origins of SARS-CoV-2” as late as 05/2020, alongside numerous NCBI data replacements and changes that happened over 02-04/2020 on all of the bat and pangolin datasets for “animal origins” leaving behind corresponding artifacts) and then as the fallback plan after 05-06/2020.

@NestCommander - Kevin W. McCairn PhD

Deliberate “Spicing up” of samples by Wuhan. Note how not even informal sources out of China have published what samples were taken in 02/01/2020, or even acknowledged the performing of sampling work in 02/01/2020 (which the existence of intensive virology-related work at the market, particularly focusing “around W7” e.g. from w6 to w8, was known only by eyewitness account by outsiders but not any official or informal acknowledgment by the operators). (Unlike 31/12/2019 which the performing of sampling work was acknowledged by the WHO report and Jiangwei, which archive.md/yyX0Z archive.md/iw1Pz archive.md/4rVph archive.md/DChUL the explicit collection of animal samples at that point ended up as negative test results that were disclosed in private channels in January 2020.) And all those trampling by contaminated boots and rubbing by contaminated suits(potentially even contaminated gloves, which unlike bare vendor hands start sterile and RNAse-free, and touches mainly surfaces just above step height as aseptic techniques becomes progressively more difficult to uphold when virology operations are performed while bowing down) are going to cause extra contamination, out of the toilets and in from then outside, particularly on all places especially those that are heavily trampled, inside this area, that were not then cleaned prior to sampling later. Reason why the only consistent positive correlation or significant mutual information between SARS-CoV-2 and species is Homo Sapiens, Despite read filtering and data obfuscation especially at that time. (No independent validation, missing method details, sometimes not even Ct values were allowed to Gao et al, only what Wuhan claimed they did and produced exclusively in-silico)

@NestCommander - Kevin W. McCairn PhD

https://archive.md/VXtu9 The actual R0 and serial interval is much, much lower and longer, contaminated by change of ascertainment criterion. Different strains spread differently. Coronaviruses superspread instantaneously and not spread continuously as HIV which FAVITES bases on. https://gab.com/Flavinkins/posts/108860074766577121 There is an inverse polytomy size to time in SARS-CoV-2. VOCs are bigger than B.1. B.1 is bigger than B. B bigger than A is expected. Unfortunately, B is in fact more transmissible and mutate faster than A…… (reason why A went extinct, and also skewed the tMRCA analysis) There is nonlinearity and an infected brain to boost. The entire assumption for pekar et al is wrong.

Flavinkins on Gab: '“ All I’m saying is that, for the sizes of the A …' Flavinkins on Gab: '“ All I’m saying is that, for the sizes of the A and B polytomies to be highly informative, I’d think one would need to make strong assumptions that suppress potential fluctuations in R0. So I don’t view this argument being very strong. I’m flying on intuition here, though.”@jbkinney “It would, and similar topologies exist (fig. below from Rambaut et al. 2020). But this is likely a moot point, as many other problematic assumptions affect the epidemic simulations and phylogenetic structures proposed by Pekar et al.”@AntGDuarte And the earliest transmission of SARS-CoV-2 is characterized by outbreak after outbreak—the majority of sampled cases came from superspreader events. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9128337/ The R0 of SARS-CoV-2 especially early on is extremely inhomogeneous—the location where an infected person becomes infectious, and the number of social contacts that happened within the infectious period, directly determines the R0. For home clusters the R0 is usually <3, where the endpoint of the transmission stops within the household (household interactions does not expand past the family itself in most households), whereas for superspreading events in crowded places the R0 could be anywhere from 40 to 100, even higher in some extreme cases like concerts, banquets or public transports. More recent examples: 2 superspreading events of Omicron BA.1 and BA.2. Cluster cases were once again substantially divergent from the rest of the world. The same topology is also observed in other, earlier superspreading clusters of Omicron BA.2.' gab.com

@NestCommander - Kevin W. McCairn PhD

Not even rumors indicated any person at all in the wildlife industry in China being sick or getting infected, not even rumors indicated direct participation with the wildlife trade (purchasing, vending, dealing, transporting, farming, butchering, cooking or eating) by any of the known official or unofficial early cases. The only ever results from these wildlife trade participants indicate perfect condition of health and no evidence of infection at all among the customers or neighbors of any of them. Not even the market cases themselves—none of them reported direct participation of the wildlife trade. And unfortunately, The only thing governing the probability for positivity of the environmental samples is “closest to the toilets” and “closest to the main entrance of the market”. In fact, the samples in the market follows the rule which a positive sample archive.md/CTP3i archive.md/ETjzS archive.md/BWZJL must be contacted by samplers.🥼👖👢=positive. archive.md/NeybM archive.md/2PM9Y archive.md/RirQ7 And not frequently handled by vendors.🥩🥬🍄🛁📻☎️📦🧺=negative. archive.md/LJzSO archive.md/4cCHG boot on surface = NGS+/PCR+, suit on sample tube = PCR-/NGS+ as it indicate contamination occurred after PCR and before NGS with alignment over the ORF1ab primer. Closer to the toilets, more likely of direct stall entry after market entry by the sampler, more samples become contaminated. Earlier the time of first sampling, the more virus in the contamination source at the entrance with less disturbance, and more virus is found in a sample that is taken from such a stall. And yes. One of the earliest unknown activity done by the WCDC including “taking environmental samples” and “cleaning” the market overnight in 31/12/2019. While the archive.md/iw1Pz animal samples have been disclosed in 01/2020 and all negative, the focus on early cases stalls in this run brought in the virus into the “live virus isolated” stalls that would be sampled in 01/01/2020. https://wwwnc.cdc.gov/eid/article/10/6/03-0852_article On the contrast, 5 independent cases with close contact to the avenues of wildlife trade for SARS-CoV-1 have happened in 4 cities in Guangdong and 1 town in Guangxi (+1 city which contact is unknown), over the same 2-months timeframe. Two of them were market workers on two independent markets which civets were sold, three of them were direct participants of the wildlife trade: two of them were civet butchers, and one a driver for wildlife dealers. All of these cases have yielded continued transmission from them. In the contrast, 0 of the early cases for SARS-CoV-2 worked in or have a history of direct participation with the wildlife industry. archive.md/yyX0Z archive.md/iw1Pz archive.md/4rVph archive.md/DChUL Exactly 0 raccoon dogs or any of the so-called “susceptible species” were found infected anywhere in the world, not even by a relative of SARS-CoV-2. archive.md/GKdtc https://archive.md/e3615 https://archive.md/vWjZl https://archive.md/nyR0q China did not put any real ban or even influence on the wildlife trade at all especially Guangdong, before the beginning of 02/2020. The first market case is in 11/12/2019. In the ~2 month time window, all 5 of the “directly wildlife linked” index SARS1 patients have already been infected. And more than half of the 11 known index SARS1 patients, over 5 of the 9 index locations. Official denial of wildlife trade did not at all influenced the real trade that was happening, which in Guangdong also proceeded all the way to the Chinese new year of 2020, which is well into February. There is no evidence at all that there is a sufficiently timely ban of wildlife trade in China to stop all and every secondary spillovers especially Guangdong.

Epidemiologic Clues to SARS Origin in China SARS Origin in China wwwnc.cdc.gov

@NestCommander - Kevin W. McCairn PhD

@NestCommander - Kevin W. McCairn PhD

@COVIDSelect All the so-called “synergy” of the Proline, the FCS, or even the N370 glycan removal, work only in VERO cells. Destruction happen when live host is used,

@NestCommander - Kevin W. McCairn PhD

archive.md/DChUL archive.md/yyX0Z archive.md/4rVph archive.md/iw1Pz https://pubmed.ncbi.nlm.nih.gov/35298912/ https://pubmed.ncbi.nlm.nih.gov/35298912/ In fact, the raccoon dogs are locally wild-caught within Wuhan, that human Herpesvirus is identified indicating human contamination have occurred alongside the clearly unique human mitochondrial reads identified, and that there are zero mutual information in term of read abundances between SARS-CoV-2 and the animals. Only the human mitochondrial reads. Worse—all of the animal species correlated perfectly with their expected viruses, and the only species which SARS-CoV-2 is the perfectly correlated expected virus is “Toilets and Homo Sapiens”. In fact, The only thing governing the probability for positivity of the environmental samples, the so-called “spatial correlation”, is “closest to the toilets” and “closest to the main entrance of the market”. Spoilers: the actual stalls that sold animals from Yunnan are entirely uninfected. It is entirely expected with zero evidence of even a single SARS-CoV-2 case linked to any of the intermediate distribution sites and secondary destinations even in Hubei or wuhan of any of the animals that were supplied to the Huanan market, especially given that the each stall have at least 3 distinct live animal suppliers for “susceptible animals” and there are 17 stalls in Wuhan, and the total number of animals sold per week is only ~58 in total. 4 animals at most per shelf life per supplier is not going to eat up the single harvest output of any farm. It will spill into other cities. None observed.

Virome characterization of game animals in China reveals a spectrum of emerging pathogens - PubMed Game animals are wildlife species traded and consumed as food and are potential reservoirs for SARS-CoV and SARS-CoV-2. We performed a meta-transcriptomic analysis of 1,941 game animals, representing 18 species and five mammalian orders, sampled across China. From this, we identified 102 mammalian-i … pubmed.ncbi.nlm.nih.gov

@NestCommander - Kevin W. McCairn PhD

Issue: the drains don’t actually have SARS-CoV-2 reads inside. Only persistent, cross-reactive animal CoVs and potential trample marks. Putting bleach onto the toilets also doesn’t help at all.

@NestCommander - Kevin W. McCairn PhD

Unlike animals including livestock, humans are neither sold nor butchered at the market. Their CoVs degraded catastrophically after 01/01/2020 and completely after 12/01/2020 leaving only artifacts behind. archive.md/13bdP archive.md/FskYn archive.md/gvHfw archive.md/4cCHG Animals that are sold and butchered at the market have their CoVs remain stable and are the only CoVs left detectable in February 2020. (note there is a continuous deposition of ratCoVs due to the rats that ran through the market nearly daily after closure (they begun to show only after 12/01/2020 when rats begun to severely infest the market). There is no possible deposition of SARS-CoV-2 or other animal CoVs by nonsampler sources after the closure of the market.(The animal CoVs that are not RatCoVs were found with consistent counts over Jan01, Jan12 and all later dates. SARS-CoV-2 rapidly decline from Jan 01 to Jan 12, then are completely gone leaving no reads that isn’t an obvious anatrifact later) This https://assets.researchsquare.com/files/rs-885194/v1/78e0e6ce-4a76-48de-9f5c-76bab452bbe6.pdf?c=1665607885 further exemplified the fact that the SARS-CoV-2 found in the market here is superfluous contamination that is distinct from the animal viruses or CoVs. One fact among many that disagree with animal origin. https://t.co/VV9Gzg7JKi? And spoilers: none of the “susceptible species in W6-29-33” (wild species that is found there and have not been rejected as unlikely susceptible experimentally) garnered a positive theil-sen estimator result in any of the slices examined. This is in addition to the fact that meaningful correlation especially ones with significant mutual information was found to animals only with animal-specific viruses, and SARS-CoV-2 to only Homo Sapiens. Why ignore the toilets again and again? W4-26-28, especially W4-28 where only 1 out of the 2/2 human cases-free positive sample have anh wildlife DNA, have the exact same cause for maximal positivity in Jan 01 as W6-29-33 in Jan 12: closest to the toilets. @jbloom_lab If you think 20 ILI samples per month can isolate the one covid case in a sea of 8000+ flu cases every two weeks. Or that pre-screened pack tube blood verified at banking to be IgM free can detect the ~100 SARS-CoV-2 IgM+ cases expected in November 2019.

@NestCommander - Kevin W. McCairn PhD

Were any corner of the WIV or even the Hankou station itself ever sampled? And well—Yunnan and Guangxi animal stalls were—Completely uninfected. https://archive.md/p3K3Z And Up to one third of all cases were moved from the lab to the market. Dazhong stopped its wildlife sales in 2014. Negative. The Yunnan and Guangxi animals are sold in W9-34-36 and W8-36-38. Negative. In deed, this is a spurious result—just like how entering the stall which you find the SARS-CoV-2 and the correlation crashed with the porcupines but kept that with the humans, theil-sen correlation give no mutual information at all to those pocrupines except a negative one with samples where SARS-CoV-2 is found. And of course, if you think that someone sick in November 2019 would not be able to meet in 15/12/2019 when the max length for sickness is merely 15 days for younger people…… Any susceptible species with significant mutual information at all and Homo Sapiens have the max mutual information. Animal CoVs are consistent in all dates not just Jan 01 and Jan 12 including after Jan 12. SARS-CoV-2 reduces rapidly in concentration from Jan 01 to Jan 12, and disappeared after Jan 12. Not inside sold animal tissues=rapidly degraded by RNAse 7. They scrambled all mutual information in 26/03/2023. Transfer contamination from the sampler labs and the toilets account for all market samples. Not vendors or animals.

@NestCommander - Kevin W. McCairn PhD

And of course, there isn’t really that an “connection” when you realized that all the “Hubei SARS” strains are in reality just HKU3 and ZC45 none with even the right RdRp or RBD, and thus the raccoon dogs, the very raccoon dogs that were being shipped to the HSM for sale, are just as expected, entirely negative at testing.

@NestCommander - Kevin W. McCairn PhD

@NestCommander - Kevin W. McCairn PhD

Initially, the toilets and the sampler pants and boots smeared the contamination out into the stalls, leading to the first set of samples which the stall with most positives samples out of all samples being always the stall that is closest to the toilets. At this time, they have also attempted to spray the stall with animals and virus as in Jan 02, when an army of hazmat suited workers performed virology work which no official or unofficial accounts for performing the work as sampling in that date was known, were identified by eyewitness records. Because the animals are all museum specimens that were far too dry to properly resuspend, the first attempt at faking “animal origin” ended up with A total absence of any consistent positive correlation or significant mutual information at all Between SARS-CoV-2 and all species other than Homo Sapiens. You can easily distinguish between common tertiary cause (confounded) from true causation by looking with increasingly finer grain of resolution, especially where the data points aren’t 0. Unlike spurious correlations from Confounding factors which ends at the resolution where the factor acts on, True causation stay correlated in every resolution and in any set of data points especially where the data values aren’t 0. In fact, confounding factors often crash in correlation quite early before that. The PREDICT ORF1ab only (RdRp) primer was used in stead of the initial “ORF1ab/N” primer set, between 27/01/2020 and 15/02/2020. https://assets.researchsquare.com/files/rs-885194/v1/78e0e6ce-4a76-48de-9f5c-76bab452bbe6.pdf?c=1665607885 The resampling of the wildlife stalls in the beginning of February 2020, within the same period, resulted in only animal-specific CoVs but no SARS-CoV-2 when amplicons generated with this primer pair were sequenced. archive.md/VNr75 archive.md/rj1pV archive.md/LJzSO archive.md/4cCHG Then, the leading hypothesis becomes snakes due to the “codon usage” paper, https://onlinelibrary.wiley.com/doi/abs/10.1002/jmv.25682 and as usual, in the continued attempt of fabricating “evidence” for whatever leading hypothesis at this time, they tried multiple ways to eliminate the human correlation edge of their initial products and dramatically oversampled their “snake stall”. They first started using Oligonucleotides and amplification products from their developing “RdRp/N/E” assay, resulting in artifact-only NGS alignments and no reads at all as these products including primer dimers generated off the test being developed and other, failed PREDICT amplification experiments, contaminates the boots, Suits and gloves of the samplers in one run and all the sample tubes used in another, with PPE in Wuhan at that point so scarce that workers often have to use the same suit for the entire day between lab work and sampling. When the E and N amplicons are present in the amplification product used, they show as single-amplicon artifacts. They also attempted verify their Q37, which the snake stall tested negative in Jan 01, but all results are failures. Facing the issue with either cross react or primer dimer and get nothing, or viral amplicon and get only amplicons with their “adulterate with amplification products” attempt (the only drain with a real SARS-CoV-2 read is a municipal sewage well on the opposite corner than the wildlife stalls!); archive.md/13bdP They decided to take samples of fresher meat from the market (animal sampling have begun in this time) that included snakes but failed to include any mammals, blend it with cell cultures and spray it onto their final sampling site “storehouse”, hoping that this would equalize out any edge humans have in correlation. The cultures https://www.ncbi.nlm.nih.gov/pmc/articles/PMC369693/ ended up far too fresh for the purported deposition date of pre-Jan01, and when the snakes are debunked, confirmed to be pure artifacts.

Synthesis and turnover of mitochondrial ribonucleic acid in HeLa cells: the mature ribosomal and messenger ribonucleic acid species are metabolically unstable. The synthesis rates and half-lives of the individual mitochondrial ribosomal ribonucleic acid (RNA) and polyadenylic acid-containing RNA species in HeLa cells have been determined by analyzing their kinetics of labeling with [5-3H]-uridine and the changes ... ncbi.nlm.nih.gov

@NestCommander - Kevin W. McCairn PhD

And also, regarding that so-called “nature’s GOF laboratory” claim—to this date, zero Sarbecoviruses with an FCS have ever been identified. web.archive.org/web/2022101805… web.archive.org/web/2022090222… In natural settings, an animal will seroconvert before the FCS can emerge, which is extremely unstable especially in D614 inside seroconverted hosts. In fact, this prevented the FCS from emerging even inside the 2002-2003 SARS-CoV-1 in the exact same hosts that the zoonati claims to be “certainly the intermediate hosts for SARS-CoV-2”, speaks volume. They were also entirely incapable of emergence without engineering “push” as demonstrated by the near neighbors which all are FCS-free and spread just fine (even better than SARS-CoV-2) without it inside all manner of hosts. gab.com/Flavinkins/pos… In fact, the destruction of the Proline at 681 associated with VOC evolution in live hosts (human or animal hosts) simultaneously remove the virus’ ability to grow efficiently in laboratory cell lines: and the very weird and hard to explain lineages show evidence of reversion to culture adaptation. In the exact same time as illegal biolabs were found and when variants emerge without a traced location of origin or epidemiological link between cases. The Proline as it turned out is important for growth in VERO cells and variants that evolved in live hosts or with P681 mutated have defect in growth inside them. This mean that Wuhan is effectively the most VERO-suitable isolated variant over the course of the pandemic. gab.com/Flavinkins/pos… gab.com/Flavinkins/pos… https://gab.com/Flavinkins/posts/108682807199122313 archive.md/az10E archive.md/TrTW5 @mbw61567742 some of the features like HV6970 also show evidence of VERO association (P2V/HL6970). Not something that you expect for ZW, as the actual host it adapted to is VERO E6. In fact, all VOCs grow less efficiently in VERO E6 compared to non-VOC/“WT”. The same in the non-D614G A.23.1 strain as well. A striking graph below. https://gab.com/Flavinkins/posts/111398506038803573 Human or mice, the variants have less growth in VERO than Wuhan. Not something you expect for a virus that was not supposed to have seen a primate before the first human infection under the market theory. The FCS look exactly like a cell line adapted version after an insertion of the ENaC FCS as expected by DEFUSE during rescue and isolation—Both direct assembly and targeted RNA recombination are viable options for its insertion, and it is not unusual for a sample or a branch of its culture to be resequenced or deep sequenced months to years after sample collection and initial operations. The CGG-CGG is also not a coincidence—using it improve immunogenicity and allow efficient killed virus vaccine production and therefore adding a self leader failsafe for deployment, and manageability in case of unintentional release. Remember those HV6970/HL6970 (VERO adaptation of P2V).

Flavinkins on Gab: 'https://journals.asm.org/doi/10.1128/jvi.00958-22…' Flavinkins on Gab: 'https://journals.asm.org/doi/10.1128/jvi.00958-22 It should be worth mentioning that unlike the N-linked glycome of other mammals, humans and cells of humans are unique in that they lacked either Neu5Gc or alpha-Gal. Bats contained the alpha-Gal epitope where other mammals contained both the Neu5Gc and the alpha-Gal epitope. https://www.sciencedirect.com/science/article/pii/S1931312820306806 An adaptation of Rhinolophid bats toward the loss of Neu5Gc synthesis (CMAH) is that they produce almost all alpha-Gal and almost no Neu5Ac on their glycocalyx. https://zenodo.org/record/5702700#.Ytew5BZ6slT Whatever HV6970 binds to, it likely abolished the ability of the S NTD to bind to alpha-Gal and favored Neu5Gc binding. Due to the anomalies found in BANaL-52 and RaTG13 (no bats in BANaL-52), and the observation that the SARS-CoV-2 S with HV6970 specifically showed greater tropism in VERO E6 cells compared to SARS-CoV S, in addition to human lung cells, and the fact that it is formed as HL6970 when GX/P2V is adapted in VERO E6, https://archive.ph/TrTW5 , and given that gaps are not counted toward identities when the SARS-CoV Urbani Spike was used as the reference for similarity alignment, the uniquely long NTD loops of the SARS-CoV-2 S and especially HV6970 should be considered a specific adaptation feature to Old-world non-human primate cells that contained Neu5Gc but not alpha-Gal. (As the NTD loop inserts are also deleted in VOCs, especially HV6970 indicating that it is not stable in humans (which don’t make either Neu5Gc or alpha-Gal) or any other species (other GX-CoVs don’t have HV6970/HL6970. Nor were GD-CoVs)). https://zenodo.org/record/6849652#.YteAXBZ6slR' gab.com
Flavinkins on Gab: 'Alpha, Delta, both have less VERO E6 than Wuhan/D…' Flavinkins on Gab: 'Alpha, Delta, both have less VERO E6 than Wuhan/D614G. Omicron, also. It is also the least VERO-growing lineage. http://virologyj.biomedcentral.com/articles/10.1186/s12985-022-01802-5 http://www.nature.com/articles/s41586-021-04266-9 http://www.sciencedirect.com/science/article/pii/S2589004221015595 This is NOT something you expect from a wildlife virus. They shouldn’t be adapted best to lab cell lines right out of the box. All adaptation trajectories (both from live mice, humans and the modified modern research cell lines) lead away from VERO E6 when an small carnivore/bat zoonosis scenario should simultaneously increase all primate tropism as the virus evolve in humans. It should raise, and not drop, VERO E6 tropism.' gab.com

@NestCommander - Kevin W. McCairn PhD

“Humanized mice will attenuate the FCS”=“humanized mice will generate the exact PRRAR site”. P681 and A372=VERO cells. And Q498=Mus Musculus germline immune system with human ACE2. Also reality: it was not “out of frame”. SARS-CoV-2 uniquely have two dS changes compared to all other QTQTNS genomes after the last Cysteine before the first S cleavage site. Shi put it in S2 And the Proline is so you can grow it into a stock in VERO E6 cells (VOCs or P681 mutants have growth defects in VERO cells) The PRRVR from mouse-passaged MERS-CoV.

@NestCommander - Kevin W. McCairn PhD

In fact, the Proline and the QTQTNS are really only stable in VERO and CaLu-3 (VERO/HAE) cells. In live hosts, P681 mutates to R681 or H681, and QTQTNS mutates to QTQTKS. There is no middle ground except if you still need to breed to stock quantity within VERO E6 cells.

@NestCommander - Kevin W. McCairn PhD

Bonus: for VERO/HAE cultures, deletions of the S1-S2 forms an equilibrium with QTQTNSPRRARS in ratios from 5% to ~70%. These mutations are actually identified within the Wuhan patients themselves, obtained from clinical samples in stead of only after culture for the first patients. Such clean QTQTN or SPRRARS deletions are not found even in homology in natural SARSr-CoVs. You only get to an FCS and the Proline (in stead of R, H or A) stabilized within these liquid medium-immersed cell cultures, and only if you start with an synthetically inserted FCS such as with the hENaC, the closest “human-specific cleavage site” to the QTQTNSRSVAS which “clear mismatches occur” at the first of the two S2 cleavages site in the Spike.

@NestCommander - Kevin W. McCairn PhD

https://gab.com/Flavinkins/posts/109640519028841414 It is not just that SARS-CoV-2 Wuhan grows best in VERO cells out of all variants. Some earliest patients harbored inside their QS specific S1-S2 deletions that can form only in VERO E6. https://gab.com/Flavinkins/posts/109888056517115303 And These seems to be related to cell surface expression that have most relevance to Spike-nanoparticles for use in non-humans. They are not vaccines that can be used in humans due to the human signal peptide used and the pcDNA3.1 which contained undesirable proteins. They are also not pseudoviruses. They best fit the “Spike nanoparticles” specified in DEFUSE out of all. (As a plain binding study would not use a complicated transmembrane anchor, which interfered with pseudovirus assembly. Human tpA signal peptide and pcDNA3.1 mean the formulation is unsafe for humans, which should not happen for such clearly finished-for-mass-production-in-HEK293f nanoparticle (that also have envelopes) formulations unless it is intended only for non-humans (such as DEFUSE bats).) https://t.co/gpv4cXu1WP. https://archive.md/1C7om

Flavinkins on Gab: 'https://journals.asm.org/doi/10.1128/JVI.00790-20…' Flavinkins on Gab: 'https://journals.asm.org/doi/10.1128/JVI.00790-20 This is not the only cell passage specific deletion that would end up in some of the very earliest patients. https://academic.oup.com/cid/article/73/2/e437/5869859 DelQTQTN, a variant that emerges during VERO cell passage, is found specifically within at least 3 patients arriving from Wuhan in Guangdong, and variations at the “upstream probe binding site” (where closest related QTQTNS-bearing genomes contained only one transition at this probe, insufficient to prevent its binding), which also emerges at cell culture passage of SARS-CoV-2 in VERO cells, are found within patient samples taken from the Central Theater Command Hospital in Wuhan, alongside with variants that had deletions in the SPRRARS site, del-mut-1. DelQTQTN(which is specifically found within the patient samples and where the deletion is exactly 1AA longer than those claimed to be in RmYN02/RacCS203/Banal-247) and variations in the upstream probe binding sites are unique to VERO cell cultured strains of SARS-CoV-2. Their presence within the earliest patient samples within China implies that a VERO cultured stock was the proximal inoculum of many of the earliest patients within China. This directly point toward the proximal origin of the Chinese outbreak being associated with virology research and culturing of viruses.' gab.com
Flavinkins on Gab: '@Parsifaler https://anandamide.substack.com/p/cu…' Flavinkins on Gab: '@Parsifaler https://anandamide.substack.com/p/curious-kittens However, examining these sequences (that were found from the Pfizer vials) revealed that while there were plasmid backbones that were highly similar, they are not identical and there were key differences between these plasmids and those that have been found within these Pseudomonas assemblies. For one, None of these plasmids harbored a mammalian/eukaryotic promoter that is used to drive the expression of the Spike protein, which is expected as these are supposed to be used to generate mRNA for vaccine production. Secondly, A duplication is discovered within one of the Pseudomonas-related sequences, whereas a deletion is found in the other plasmid with the Spike protein, while substitutions within the backbone fragment are found within both of the P.aeruginosa-linked plasmid sequences. This indicates that, compared to these unknown primary backbone sequences, the P.aeruginosa assemblies were found to have undergone a substantial level of evolution that involved duplication, deletion, and substitutions within the backbone sequences for these plasmids--suggesting an ancient origin of the plasmids found within the Pseudomonas assemblies. Third, The C-terminal of the Spike protein inserts, when found, were found to be native--expected for an mRNA vaccine. One of the proteins found was an S1-only protein. The C-terminal membrane anchor coiled-coil sequence is not found in any of these plasmids. This rules out mRNA vaccination as the origin for these Pseudomonas-linked plasmid sequences. Finally, While many P.aeruginosa strains are resistant toward Neomycin and Kanamycin, Neo/Kan, not found within the chromosomes of these assemblies, does in fact additionally confer GmR (Gentamicin resistance) toward the P.aeroginosa strains. While it is possible that the plasmids may have derived from contamination within the BioProject, the supposed natural host for these plasmids, laboratory strains of E.coli, was nowhere to be seen within the BioProject PRJNA839565. The closest sequences found within the BioProject by BLAST just returned other strains of Pseudomonas, Sampled in China in 2019. https://www.ncbi.nlm.nih.gov/nucleotide/CP081287.1?report=genbank&log$=nucltop&blast_rank=1&RID=Z2K3722G01R https://archive.md/hm8zm https://archive.md/kkSkI https://archive.md/O9Kkr https://archive.md/LYema https://www.ncbi.nlm.nih.gov/nucleotide/JAMOHA010000033.1?report=genbank&log$=nucltop&blast_rank=1&RID=Z2M30VUK01R https://www.ncbi.nlm.nih.gov/nucleotide/JAMOGL010000063.1?report=genbank&log$=nucltop&blast_rank=2&RID=Z2M30VUK01R https://www.ncbi.nlm.nih.gov/nucleotide/JAMOGK010000062.1?report=genbank&log$=nucltop&blast_rank=3&RID=Z2M30VUK01R https://archive.md/GTo6k This also included contigs that have very low coverage, supposedly consistent with "contamination origin". This indicates that 1: the plasmids found have evolved substantially compared to their closest ancestors within the labs. 2: they are found in Pseudomonas spp., instead of laboratory origin E.coli. This is expected as mammalian expression vectors with the use of SV40 Ori and CMV promoter is conventionally maintained within the lab using AmpR, and one lab that deals with mammalian expression typically maintain their plasmids using only one antibiotic for convenience in the preparation of the medium necessary for culturing the bacterial host. https://www.ncbi.nlm.nih.gov/nuccore/JAMOGH010000091.1 https://archive.md/ERB08 While in the U.S., AmpR may have been avoided in vaccine preparation (mRNA production vectors instead of mammalian expression vectors) within the Moderna facilities, the same basic rule for non-bacterially-oriented vectors, one antibiotic resistance gene per lab, is maintained within these vaccine-derived Spike expression vectors (all vectors found within the vaccine vials used Neo/Kan and nothing else). This is distinct from that is seen in the Pseudomonas assemblies, where Eukaryotic-oriented Neo/Kan accompanied with AmpR in only one of the plasmid versions was found. This also indicates that both mutations (substitutions seen in the backbone), duplications/deletions, and recombinations have shaped the plasmid backbone found within these P.aeruginosa assemblies and that this did not happen within a lab (E.coli is not found within PRJNA839565).' gab.com

@NestCommander - Kevin W. McCairn PhD

In fact, the XRRXRX motif is considered a signature of cell culture adaptation, in stead of live host adaptation which the Heparan sulfate-binding motif is invariable broken. journals.asm.org/doi/10.1128/JV…

@NestCommander - Kevin W. McCairn PhD

Initially they do not have sufficient samples for an MRCA analysis, and that they were satisfied with only lineage B being available at the market. However, Eventually it was found out that lineage A is the more ancestral strain, and they have to make up a sample to put it into the market. https://archive.md/ANS4Q they came up with “A20”, inconsistent in both the ratio of 8782/28144 and in the ratio of reads vs Ct values with the other samples they claimed to show. The way they adulterated the post-26-03/2023 datasets is also one of the reason why the jbloom et al datasets gets humans as higher ranked in the alignments in the positive samples compared to all samples in both all sampling dates and Jan 12—They do it by dropping random human reads into the “negative samples” and scrambling the rest of the animal reads, all uploaded after 26/03/2023, resulting in an reduction of spread of correlation metrics over all species and correlatedness with humans for all samples compred positive samples only, not only in Jan 12 but for all sampling dates. In fact, all 3 samples that are different between 2021 and 2023 are also samples that have additional datasets uploaded in 26/03/2023 after an 03-10/03/2023 upload. Sample A20 have distinct host composition between the 03-10/03/2023 (without lineage reads) and 26/03/2023 upload, which is not expected from “viral amplicon sequencing” (with lineage A reads) which does not perturb the host reads if genuinely from the same sample. This is consistent with the general scrambling of host sequences within the “post-26/03/2023” samples, and showcases irreconcilable dishonesty within this sample set especially when sample B5, likely used as a standard, remain unchanged, creating an additional inconsistency in term of protocols—one set of numbers in 2021, one set of numbers in 03/03/2023, and a third set of numbers in 26/03/2023. Zero custody in the CCP’s grasp up to upload, change constantly per demand of the leading zoonosis theory—“change those ‘data’ on the fly, based on any reactions and feedback, make up your uploads to attempt pushing zoonosis as hard as possible”. This is also why the mutual information between SARS-CoV-2 and any species at all, especially all land-dwelling species, are completely destroyed upon inclusion of the 26/03/2023 upload date. These are the species that they seek to scramble reads in order to remove the correlational edge of Homo Sapiens that have remained despite their attempt at filtering their previous “data”. In Mar-Apr 2020, China officially blamed wild animals sold in the Huanan market. Publishing the “data” as currently seen to Holmes would be the best way to solidify this then-official opinion. If the “market environmental data” can be interpreted in any way to arrive at the C-C “conclusions”, ECH won’t be denied of it. Since he is denied, the most logical reason for the denial is that it does not originally support any of the C-C “conclusions”, and were tampered only recently to poison the scientific database and to provide a fallback for debate purposes. Only after evident in-vitro and in-silico tampering and subsequent approval by the CCP, would it be officially permitted—in fact, actively given to Holmes for “analysis”. Despite attempts at scrubbing all 300nt+ non-viral human Contigs from the “positive wildlife stall samples”, which have led to an inverse correlation between the 300nt+ contigs left inside these samples and the product of Homo Sapiens and SARS-CoV-2, mutual information and the ratio between the leftover human mitochondrial reads and SARS-CoV-2 have been preserved as the removal process preserved ratios, And you still end up with Homo Sapiens being the most mutually informant species for SARS-CoV-2 whenever significant mutual information is preserved at all within a slice of the “dataset”.

@NestCommander - Kevin W. McCairn PhD

In fact, there are even further inconsistencies in these so-called “data releases” which further indicate that there are both sufficient number of unaccounted flow cells to source all the reads necessary for scrambling the post-28/03/2023 “datasets” and that there are likely both selective representation of and cannibalization-and-redistribution of sample datasets, with once again a total absence of custody information or in deed, from what mix of material was the actual source libraries constructed from, suggested that the CCP used a strategy of “holding back as many reads as that would be needed to adjust the datasets to whatever direction to promote the arrival at a “likely zoonosis” conclusion so there is always a conclusion to jump to if the lab is indeflectably blamed” when posting the “dataset” or the associated publications—not even the number of samples per category could be matched to the percentages published. (see how the mutual information metric, e.g. the plausibility to use regression from the other points on the correlation graph to identify the location of a dot on the graph from only one axis of its coordinates e.g. the ability to predict the concentration of virus from species and vice versa, are completely crashed upon inclusion of the “26/03/2023” samples in both Jan 12 and all collection dates)

@NestCommander - Kevin W. McCairn PhD

https://archive.md/VNr75 In fact, contrary to the claim that they are “near the market”, all 3 of the lineage A samples in Early Wuhan was actually found in tight and direct linkage with the WCDC much more than and as opposed to the Huanan market. One is A20, the WCDC sampler PPE which would be among the first sample to replace outright in stead of merely filtering and scrambling to minimize leak of lab-linked information. Exactly what to make it for best fit to the running theory, however, they stumbled and changed twice with feedback and changing demands, resulting in three distinct and dishonestly-inconsistent-with-each-other datasets and results. Another stayed in a Hotel, which is right next-door to the “new” WCDC site where samples and cultures would have been transported to and workers would move back and forth between when they have just finished setting up the new lab and have started experimentation which needed materials likely exist in both the old and the new site at the beginning of its operation. The third, “cluster 1”, is right on the route of this back and forth commutation and near the “old” WCDC site, “somewhere near the Xinhua hospital”. All have strong linkage to the WCDC and none documented credible linkage to transmission at the Huanan market. The WCDC and the Hubei CDC stores all of the human samples and backups of research cultures of pathogenic microbes in Wuhan, as this is their legally delegated duty (the “各级疾控部门” are termed “保藏机构” for “病原性微生物” under Chinese law governing the use of cultures and samples of human and animal pathogenic microbial samples, and samples that were suspected to have the possibility of containing such microorganisms. These are also the only locations which first round samples arriving in Wuhan are allowed to go for pre-screening prior to entry into the other labs in Wuhan, “检测机构”. ) and that labs in China are not allowed to store such cultures except several select state key laboratories. Since 2014, the only EID surveillance target in Wuhan is the HSM which all other sites are kept blind so that they can blame Huanan in case the research labs suffer an accident. It is likely that the WCDC (but not the Hubei CDC) would internally get the wind of an “SARSr-CoV” (with an antigen kit that were apparently available to many high-level hospitals in Wuhan) almost the soon their surveillance program is tripped in 20-22/12/2019 with their first hospital-visited market case. After an initial release from the WIV that caused Chen’s infection, and eventual transmission to the HSM via line 2 of the Wuhan metro, they mobilized the WCDC in 20-22/12/2019 to begin tapering with the environmental samples (largely based on the leading zoonosis theory proposed or identified at that time) and prepare for any needed scapegoat action. That mobilization ended up causing an infection of a WCDC worker with an aliquot of a sample containing WA1, A and B in the same quasispecies, which then go on infecting all of the earliest lineage A cases in Wuhan.

@NestCommander - Kevin W. McCairn PhD

The WHO report is found to have moved all https://gab.com/Flavinkins/posts/109048819612838694 cases with onset before 27/12/2019 https://www.researchgate.net/publication/370635299_Greater_than_the_Sum_of_its_Parts_-_Aggregated_Wuhan_COVID-19_case_data_points_to_the_wrong_side_of_the_Yangtze_River_-_Rixey_-_20230509 inside the district of Wuchang, and then all cases within the central Wuchang prefectures and those prefectures near the labs, to Jianghan. with calibration performed so that they would add up to a perfect “bullseye” https://www.reuters.com/article/us-health-coronavirus-who-china-idUSKBN2AD090 https://www.nytimes.com/2021/02/12/world/asia/china-world-health-organization-coronavirus.html https://www.washingtonpost.com/opinions/2022/11/17/covid-early-cases-wuhan-china-mystery/ https://www.wsj.com/amp/articles/china-refuses-to-give-who-raw-data-on-early-covid-19-cases-11613150580 of within 50m precision at the Huanan market, which would not be realistically possible given the expected social biases from uneven residential densities even in the neighborhood of the Huanan market. Cases *residences* were dropped into water and placed into non-residential areas as the result of this tampering, especially the former which, residences could not exist on since they would be washed away by the water. Obvious examples included accountant Chen, which they refused to mention where he lived at all since the WHO report alongside any specific single cases, and one of the two “江夏急救中心” ambulances seen blaring into Wuchang in 31/13/2019, where only one out of the two, one that likely did not live in Shidong, were counted as a dot in Jiangxia. They refused to give any line lists at all for a reason.

ResearchGate - Temporarily Unavailable researchgate.net
On W.H.O. Trip, China Refused to Hand Over Important Data (Published 2021) The information could be key to determining how and when the outbreak started, and to learning how to prevent future pandemics. nytimes.com
Opinion | Wuhan’s early covid cases are a mystery. What is China hiding? The story of how the pandemic got started — and turned into a global catastrophe — remains a black box. It should not be. washingtonpost.com

@NestCommander - Kevin W. McCairn PhD

Tampering with data, moving cases, contradicting own early media reports, this is the reality of that “market centered” early cases “dataset”. The “dataset” is as fake and as inconsistent as a $3 bill. https://gab.com/Flavinkins/posts/109256201942085712 They completely eliminated all cases that landed within the borders of the Wuchang district before 27/12/2019, to the point that they claim to see cases further east of but not within Wuchang. This is the cluster that they were hiding. Again, the early cases are WA1, which even when cluster, failed to meet the recognition requirements. https://gab.com/Flavinkins/posts/109248812361151175 The “中部战区总医院” reported that they were taking in 700+ cases in a single day at 31/12/2019 all in the fever clinics. https://gab.com/Flavinkins/posts/109400051863347812 Then there is a media coverage bias.

Flavinkins on Gab: 'https://gab.com/Flavinkins/posts/1088805315972559…' Flavinkins on Gab: 'https://gab.com/Flavinkins/posts/108880531597255968 https://gab.com/Flavinkins/posts/109147956977669077 Also, remember accountant Chen? (Why his dot was moved to the WCH if he lives in Wuchang/Jiangxia?) it turned out that it was not only his dot that was not in the right place. Every dot within the 2km radius of the Wuchang railway station was moved or removed. This is within the area that is expected to have the infectious disease and respiratory cases ultimately serviced by the “中部战区总医院”. The hospital that sees large-scale respiratory case anomalies the first in Wuhan on official records, where the decision to “enter battle stations” on 01/01/2020 was made because of an “unexpected and fast-growing anomaly in the respiratory disease surveillance data” beginning at least as late as 31/12/2019. There were dots that were east of this area, and there were dots that were south of this area, in locations with lower population density compared to downtown Wuchang and further from the market. (2 ambulances from Jiangxia on 31/12/2019, but only 1 dot on the WHO map and he wasn’t accountant chen…… (likely ascertained by contacting a HSM case on public transport, “试行诊疗方案”) (accountant Chen got to the WCH in 27/12/2019)) Considering how cases that were admitted to the “中部战区总医院” weren’t directly reported except for WH01 in 14/01/2020, (Only 1 out of the 4 known sequenced cases here were directly reported. WH03 is reported after transfer to the Zhongnan hospital, one of the 2 initial market cases reported in the location. WH02 and WH04 were not in the NNDRS dataset and displayed as “unknown” in the WHO report) and how they then report seeing more fever cases in a single day than the entire CDC pre-04/01/2020 onset dataset (the point when they have to expand their fever clinics), it is quite likely that cases that initially broke out in Wuchang were muted by admission into a hospital that is placed under a command that doesn’t have to report on the NNDRS, and that any cases found in Downtown Wuchang had their “residential addresses” altered to place them as close to the Huanan market as possible and out of the Wuchang area. This would not be the first time when cases that came from an “inconvenient” location were hidden inside PLA-operated hospitals to prevent them from being counted. https://gab.com/Flavinkins/posts/109701931477090563 Why the WMHC rejected the WHO’s demand for line listings of the 174 “NNDRS cases” in annex E2? Also, one dot in Jiangxia is one of the two https://gab.com/Flavinkins/posts/109048819612838694 ambulances that were seen in 31/12/2019 from Jiangxia. Only one become a dot (central Jiangxia as opposed to the Shidong prefecture). It is possible that this is Chen’s relative that “visited a local market”, meaning that this is a case that is ascertained by contact with an early case, and saved from removal because of post-27/12/2019 onset. No dot at all is inside the borders of the WuChang district, even when dots begin showing up east of it in less populated places further from the market. This is clearly artefactual, indicating attempt at breaking up the cluster in Wuchang.' gab.com
Flavinkins on Gab: 'https://archive.ph/yu2Jg Now, see this. Remember …' Flavinkins on Gab: 'https://archive.ph/yu2Jg Now, see this. Remember that “中部战区总医院”? “ 2019年12月31日,武汉市卫健委首次公开发布通报,确认多例肺炎病例。   与此同时,中部战区总医院发热门诊人数陡增,最高时一天超过600人。江晓静、王琼书、刘孟丽等一批中部战区总医院专家,感觉到病情凶险。   作为全军急性呼吸道传染病病原监测参比实验室,中部战区总医院负压实验室是驻汉部队唯一带有负压功能的实验室。从2015年起,为监测流感、呼吸道腺体和其他传染病,他们每年冬春季都会紧密监测发热患者情况,并进行跟踪和采样。   但今年的数据变化太快太突然,没有任何征兆。   经中部战区总医院感染科、疾控科等科室专家和医护人员共同讨论研究,提议马上启动《呼吸道传染病防控方案》。中部战区总医院党委专题召开分析会,形成共识。   医院成立领导小组,主官亲自挂帅;抽调精兵强将,核心部门全员参与;对发热门诊、传染科等关键环节开展防护培训,采取隔离措施,提高防护等级;加紧储备口罩、防护服、隔离衣等防护器材,紧急采购30个正压呼吸器和60个备用滤芯。同时,向驻汉驻鄂部队进行传染病防护提醒。   今年元旦,中部战区总医院进入临战状态。   1月4日,医院调整扩大发热门诊,全院提高一级防护等级。   1月6日,开始收治第一例患者。几天后,传染科床位告急。   1月15日,医院决定火速扩建传染病区。   1月16日,向武汉市卫健委送检第一例样本病例。   1月17日,抢建的传染2病区、3病区开放。   1月19日,医院提升疫情防控指挥等级,成立一线指挥部,党委成员集体住进办公楼;机关各部门重新进行人员编组和任务分工;专家组、医疗组、保障组以及各预备队抽组完毕;全院进行传染病防治专业培训考核。” Then “新冠肺炎被纳入乙类传染病、采取甲类措施严格管理。而中部战区总医院发热门诊从一开始就采取了高一级的防护措施,严格按照甲类传染病进行处置和管理。   随后,疑似病例数、确诊病例数、死亡病例数不断攀升,治愈人数却始终显示着“0”。” Keep in mind, the listing of “Novel coronavirus infected pneumonia(NCIP)” as a “class B infectious disease” is in 20/01/2020. Standard 1, from 15-17/01/2020, and the “试行诊疗方案” before it, require “unsuccessful antibiotics treatment” and “unsuccessful treatment using a panel of “standard antibiotics”” for cases that didn’t have exposure history to the Huanan market. This mean that most if not all the cases that can be ascertained as being “NCIP” or “VPUE” by the standards at that time have to be in the severe non-self-limiting group. 06/01/2020 is exactly 3 days after they begin case ascertainment according to the “试行诊疗方案” they received in 03/01/2020, through the use and monitoring of antibiotics treatment on fever patients. Just after “a few days” (“3-5 days”) of they begin to ascertain cases for isolation, “the infectious disease wards begin to run out of beds”. So many fever patients floods the hospital that they begin to “expand the fever clinics” at 04/01/2020. And what caused them to begin “今年元旦,中部战区总医院进入临战状态”? “Enter battle stations at 01/01/2020”? “On December 31, 2019, the Wuhan Municipal Health Commission issued a public notice for the first time, confirming a number of pneumonia cases.   At the same time, the number of fever outpatients in the General Hospital of the Central Theater has increased sharply, with more than 600 people a day at its peak.A group of experts from the General Hospital of the Central Theater, including Jiang Xiaojing, Wang Qiongshu, and Liu Mengli, felt that their condition was dangerous.   As a reference laboratory for the monitoring of the pathogen of acute respiratory infectious diseases throughout the army, the negative pressure laboratory of the Central Theater General Hospital is the only laboratory with negative pressure function of the troops stationed in Han.Since 2015, in order to monitor influenza, respiratory glands and other infectious diseases, they have closely monitored patients with fever every winter and spring, and followed up and sampled them.   But this year's data changes too quickly and suddenly, without any signs.” Indicated by the ENA reservation dates, this begun at least on 10/12/2019. Out of all the cases that they accept into their isolation wards, “suspected cases, confirmed cases and deaths keeps raising up, but “recovered cases” remained 0”. All isolation ward/infectious disease ward cases were ascertained according to the standards that were then official in Wuhan. Self-limiting cases were excluded. This is also how WH01-WH04 were sent to the BGI. Samples were saved from all fever and respiratory cases admitted to the hospital, and when they received a command for “continued epidemiological investigation in several hospitals (near Huanan market), Huanan market and the neighborhood of Huanan market” in 31/12/2019, 4 cases from the hospital that satisfied the “from or near the market” criterion were selected, and samples sent for analysis at availability (when BALF sampling from bronchoscopy is performed). Only WH01 with a sample that is available in 26/12/2019 would be reported to the WMHC and enter the WHO dataset, as the military commanded General Hospital of the Central War Zone seems to be only disclosing case data to other institutions after they have a result on the cases first (where the first case ascertained with the “试行诊疗方案”, sometimes in 06/01/2020, only had the sample sent to and reported to the WMHC in 16/01/2020.). WH03 reported in Zhongnan. Confusion on the identity and nature of WH01-WH04 would continue even until today. https://zenodo.org/record/4119263/#.Y1yAtCW8klQ At its peak, the General Hospital of the Central War Zone were receiving 600 cases a day from its fever clinics—more than twice the total reported cases by onset (CDC) at the time when so many cases were flooding the fever clinics that they have to “expand the fever clinics in 04/01/2020”. This is consistent with the eyewitness report on dozens of times 🚑, at least 2 from Jiangxia (remember only 1 dot on the WHO map was from Jiangxia) rushing across the ShiPaiLing road leading to the “中部战区总医院” in 31/12/2019.' gab.com
Flavinkins on Gab: 'A media coverage bias also exists. The “story” of…' Flavinkins on Gab: 'A media coverage bias also exists. The “story” of the cluster 1 report was propped up by CCP state-owned media. This media coverage would only go to the doctor that reported the first officially recognized cases. Any other doctors that reported case clusters elsewhere in Wuhan, because they didn’t report a market case, and because the CDC recognized only the HPHICWM report (grouping it into the later VPUE category) only because of and after the HSM cases were being reported, and when the specific surveillance targeting the HSM at the WCH reported to them the genome of a SARSr-CoV (in 29/12/2019), they didn’t get recognized even if a similar report is given—brushed aside into the 92 CDC-searched and NNDRS-discard cases. No CCP media coverage would land on these doctors, and their story would remain buried just like the 92 cases (that may even included cases that they reported) discarded from the WHO report.' gab.com

@NestCommander - Kevin W. McCairn PhD

Myanmar, Cambodia, which also, incidentally, was where China specifically sampled before. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7818139/ This also included human sampling as well, per “pathogen host adaptation and immune intervention”. Now where I find these specific human Mitochondrial haplotypes again? (Southeast Asia, not central China, +SARS-CoV-2, no FCS, exactly where the WIV and the WHU would sample humans and put the resulting cultures into the WCDC). Note how the China serological sampling is a specific sampling around bat caves which human cells-infecting CoVs have been specifically isolated before. Notice how low this number is compared to southeast Asia (Cambodia, Myanmar). Despite Shi and Daszak’s name on it, “no work was ever conducted in Laos” they claimed. How can the EHA be trusted?

Decoding the RNA viromes in rodent lungs provides new insight into the origin and evolutionary patterns of rodent-borne pathogens in Mainland Southeast Asia As the largest group of mammalian species, which are also widely distributed all over the world, rodents are the natural reservoirs for many diverse zoonotic viruses. A comprehensive understanding of the core virome of diverse rodents should therefore ... ncbi.nlm.nih.gov

@NestCommander - Kevin W. McCairn PhD

Not only Leaked SRA data included both the exact kind of viruses that they claim will not be present in the WIV—and the exact SARS-CoV-2, WA1, cultured in a CoV-specific tailored fusion cell line VERO-CHO never used in China and sequenced before even a sample of WA1 can be taken in China, alongside C/C and B, at high passage depths, and contained within it residual human DNA not from anywhere in central China but in stead right where they were sampling from the 2018 “pathogen host adaptation and immune intervention” grant—the belt and road regions; https://gab.com/Flavinkins/posts/109888056517115303 But also these membrane anchored cellnsurface expression vectors intended for HEK293f of the SARS-CoV-2 Spike that have most relevance to Spike-nanoparticles for use in non-humans. They are not vaccines that can be used in humans due to the human signal peptide used (expressing an antigen together with a human protein, especially when co-localized through generation of nanoparticles processed from the same peptide chain) and the pcDNA3.1 which contained undesirable proteins. They are also not pseudoviruses. They best fit the “Spike nanoparticles” specified in DEFUSE out of all. (As a plain binding study would not use a complicated transmembrane anchor, which interfered with pseudovirus assembly. Human tpA signal peptide and pcDNA3.1 mean the formulation is unsafe for humans, which should not happen for such clearly finished-for-mass-production-in-HEK293f nanoparticle (that also have envelopes) formulations unless it is intended only for non-humans (such as DEFUSE bats).) https://t.co/gpv4cXu1WP. https://archive.md/1C7om Continued EHA human sampling=Yunnan and belt and road DNA. Isolate if possible=special unpublished VERO-CHO cells. And it was sequenced before the first public sequencing of SARS-CoV-2 with this machine type by the flow cell, confirmed via Sangon policy and Chinese law, and before+not matching any samples of WA1 was even taken in China. And this exact CAS special project mirroring of DEFUSE+Year 5 extension—sample humans from belt and road area, isolate and engineer viruses for infection characterization, and create vectorized and nanoparticle vaccines that are capable of bringing in both backbone and Spike into bats studied in and released by the WIV, and into the main sample storage facility of the WCDC. (Also see this—note all the FCS relevant oddities can also be caused by targeted RNA recombination link.springer.com/chapter/10.100… followed by cell culture). The instability associated with 8782/2814/18060 (WA1->A->B) is found to recur at least 3 times in the WA1/UW cluster, especially their cultured isolates. The associated samples have T22657C, T3346C, A21562C and G487T. all of which is in RaTg13 but not in WuHu-1. also T1963C and T22963C in BANAL-52. https://gab.com/Flavinkins/posts/109640519028841414 It is not just that SARS-CoV-2 Wuhan grows best in VERO cells out of all variants. Some earliest patients harbored inside their QS specific S1-S2 deletions that can form only in VERO E6.

Flavinkins on Gab: '@Parsifaler https://anandamide.substack.com/p/cu…' Flavinkins on Gab: '@Parsifaler https://anandamide.substack.com/p/curious-kittens However, examining these sequences (that were found from the Pfizer vials) revealed that while there were plasmid backbones that were highly similar, they are not identical and there were key differences between these plasmids and those that have been found within these Pseudomonas assemblies. For one, None of these plasmids harbored a mammalian/eukaryotic promoter that is used to drive the expression of the Spike protein, which is expected as these are supposed to be used to generate mRNA for vaccine production. Secondly, A duplication is discovered within one of the Pseudomonas-related sequences, whereas a deletion is found in the other plasmid with the Spike protein, while substitutions within the backbone fragment are found within both of the P.aeruginosa-linked plasmid sequences. This indicates that, compared to these unknown primary backbone sequences, the P.aeruginosa assemblies were found to have undergone a substantial level of evolution that involved duplication, deletion, and substitutions within the backbone sequences for these plasmids--suggesting an ancient origin of the plasmids found within the Pseudomonas assemblies. Third, The C-terminal of the Spike protein inserts, when found, were found to be native--expected for an mRNA vaccine. One of the proteins found was an S1-only protein. The C-terminal membrane anchor coiled-coil sequence is not found in any of these plasmids. This rules out mRNA vaccination as the origin for these Pseudomonas-linked plasmid sequences. Finally, While many P.aeruginosa strains are resistant toward Neomycin and Kanamycin, Neo/Kan, not found within the chromosomes of these assemblies, does in fact additionally confer GmR (Gentamicin resistance) toward the P.aeroginosa strains. While it is possible that the plasmids may have derived from contamination within the BioProject, the supposed natural host for these plasmids, laboratory strains of E.coli, was nowhere to be seen within the BioProject PRJNA839565. The closest sequences found within the BioProject by BLAST just returned other strains of Pseudomonas, Sampled in China in 2019. https://www.ncbi.nlm.nih.gov/nucleotide/CP081287.1?report=genbank&log$=nucltop&blast_rank=1&RID=Z2K3722G01R https://archive.md/hm8zm https://archive.md/kkSkI https://archive.md/O9Kkr https://archive.md/LYema https://www.ncbi.nlm.nih.gov/nucleotide/JAMOHA010000033.1?report=genbank&log$=nucltop&blast_rank=1&RID=Z2M30VUK01R https://www.ncbi.nlm.nih.gov/nucleotide/JAMOGL010000063.1?report=genbank&log$=nucltop&blast_rank=2&RID=Z2M30VUK01R https://www.ncbi.nlm.nih.gov/nucleotide/JAMOGK010000062.1?report=genbank&log$=nucltop&blast_rank=3&RID=Z2M30VUK01R https://archive.md/GTo6k This also included contigs that have very low coverage, supposedly consistent with "contamination origin". This indicates that 1: the plasmids found have evolved substantially compared to their closest ancestors within the labs. 2: they are found in Pseudomonas spp., instead of laboratory origin E.coli. This is expected as mammalian expression vectors with the use of SV40 Ori and CMV promoter is conventionally maintained within the lab using AmpR, and one lab that deals with mammalian expression typically maintain their plasmids using only one antibiotic for convenience in the preparation of the medium necessary for culturing the bacterial host. https://www.ncbi.nlm.nih.gov/nuccore/JAMOGH010000091.1 https://archive.md/ERB08 While in the U.S., AmpR may have been avoided in vaccine preparation (mRNA production vectors instead of mammalian expression vectors) within the Moderna facilities, the same basic rule for non-bacterially-oriented vectors, one antibiotic resistance gene per lab, is maintained within these vaccine-derived Spike expression vectors (all vectors found within the vaccine vials used Neo/Kan and nothing else). This is distinct from that is seen in the Pseudomonas assemblies, where Eukaryotic-oriented Neo/Kan accompanied with AmpR in only one of the plasmid versions was found. This also indicates that both mutations (substitutions seen in the backbone), duplications/deletions, and recombinations have shaped the plasmid backbone found within these P.aeruginosa assemblies and that this did not happen within a lab (E.coli is not found within PRJNA839565).' gab.com
Flavinkins on Gab: 'https://journals.asm.org/doi/10.1128/JVI.00790-20…' Flavinkins on Gab: 'https://journals.asm.org/doi/10.1128/JVI.00790-20 This is not the only cell passage specific deletion that would end up in some of the very earliest patients. https://academic.oup.com/cid/article/73/2/e437/5869859 DelQTQTN, a variant that emerges during VERO cell passage, is found specifically within at least 3 patients arriving from Wuhan in Guangdong, and variations at the “upstream probe binding site” (where closest related QTQTNS-bearing genomes contained only one transition at this probe, insufficient to prevent its binding), which also emerges at cell culture passage of SARS-CoV-2 in VERO cells, are found within patient samples taken from the Central Theater Command Hospital in Wuhan, alongside with variants that had deletions in the SPRRARS site, del-mut-1. DelQTQTN(which is specifically found within the patient samples and where the deletion is exactly 1AA longer than those claimed to be in RmYN02/RacCS203/Banal-247) and variations in the upstream probe binding sites are unique to VERO cell cultured strains of SARS-CoV-2. Their presence within the earliest patient samples within China implies that a VERO cultured stock was the proximal inoculum of many of the earliest patients within China. This directly point toward the proximal origin of the Chinese outbreak being associated with virology research and culturing of viruses.' gab.com

@NestCommander - Kevin W. McCairn PhD

So, where are DEFUSE going to sample humans? Also, the RaTg13 RBD bind human ACE2 poorly, resulting in exceptionally high sVNT cross-reactivity as even poorly binding antibodies can display ACE2 off it. Once again, 1: RaTG13 is not viable. https://zenodo.org/record/5702700#.ZJ2KiyV6slT https://zenodo.org/record/5778318#.ZJ5hyCV6slT 2: the real issue is that 1. WIV lies about everything serological. None of their “tests” were positive when politics require it to be negative. And 2. The missing sequences of Latinne et al is where you find what the WIV was working on. 7 SARSr and 54 total CoVs were missing entirely.

Anomalies in BatCoV/RaTG13 sequencing and provenance To this date, the most critical piece of evidence on the purposed “natural origin” theory of SARS-CoV-2, was the sequence known as RaTG13, allegedly collected from a single fecal sample from Rhinolophus Affinis. Understanding the provenance of RaTG13 is critical on the ongoing debate of the Origins of SARS-CoV-2. However, this sample is allegedly “used up” and therefore can no longer be accessed nor sequenced independently [1], and the only available data was the 3 related Genbank accessions: MN996532.1, SRX7724752 and SRX8357956. We report these datasets possessed multiple significant anomalies, and the provenence of the promised claims of RaTG13 or it’s role in proving a “probable bat origin”[2] of SARS-CoV-2 can not be satisfied nor possibly be confirmed. zenodo.org
The seminal paper from the Wuhan Institute of Virology claiming SARS-CoV-2 probably originated in bats appears to contain a contrived specimen, an incomplete and inaccurate genomic assembly, and the signature of laboratory-derived synthetic biology The bat coronavirus RaTG13 was purportedly identified in a bat “fecal” specimen that is probably not feces, has significant unresolved method-dependent genome sequence errors and an incomplete assembly with significant gaps, and has an anomalous base substitution pattern that has never been seen in nature but is routinely used in codon-optimized synthetic genome constructions performed in the laboratory. zenodo.org

@NestCommander - Kevin W. McCairn PhD

Note how the WHU itself gets about half of all the animal work that involved the “understanding risk of bat coronavirus emergence” grant— Both it and DEFUSE are included in the “pathogen host adaptation and immune intervention” grant.

@NestCommander - Kevin W. McCairn PhD

@NestCommander - Kevin W. McCairn PhD

And in deed, this 2018 EHA grant approval document invoked an near exact replication of DEFUSE on what to do with the new SARSr-CoVs—gather Spikes 10%-25% divergent from SARS-CoV-1, test with chimeric viruses first, and then take the immune-evasive and human infectious ones, and validate with full genomes—clearly more than “only 1-2” with binding to human cells first, see SARS-like signs from some of these next, and then some (more than 1 but nor all) don’t respond to mAbs, vaccines, etc.

@NestCommander - Kevin W. McCairn PhD

@BlackTomThePyr8 - Tom Czerniawski

Few will ever understand the gravity of what Lt. Col. Murphy did. By releasing DEFUSE he showed us where to look. From that discovery, subsequent draft DEFUSE documents were discovered years later by @USRightToKnow that proved C-19 to be synthetic, and revealed how it was made.

@NestCommander - Kevin W. McCairn PhD

What those analyzers think: “many different precise and specific pieces recombine into SARS-CoV-2”. Reality: an unpublished but readily sampled SARS-CoV-2 progenitor spread fragments over time into multiple locations. Some end up in the published samples. “10%-25% Spike divergence”—they will isolate RBDs from viruses that they sample to identify one that bind ACE2. Only fine tuning is needed later. “Exotic recombination”? It is just reverse transcriptases in cases of “postpandemic inserts”. How many billion hosts are needed and how this compare to the total number of wild animals on the entire Earth? link.springer.com/chapter/10.100… Use targeted RNA recombination if you have a cultured virus in stead. “Perfect synergy”? Nothing but VERO/HAE cells. Those “synergy” are only stable here. Not in any live hosts which they will mutate and destroy each other in stead. https://gab.com/Flavinkins/posts/109205261283826972 ReCCA is tautological and fictitious. https://gab.com/Flavinkins/posts/109863181504837302 https://gab.com/Flavinkins/posts/109465063042828622 https://gab.com/Flavinkins/posts/109255356915252021 BtSY2 is sequenced in 2018. https://gab.com/Flavinkins/posts/109399710986742685 BANAL is in the hands of the DOD in 2017. https://gab.com/Flavinkins/posts/109340247585238829 https://gab.com/Flavinkins/posts/109800300869616862 And all of which got funneled into the EHA, which eventually will end up in the WIV.

Flavinkins on Gab: 'https://gab.com/Flavinkins/posts/1089460581208001…' Flavinkins on Gab: 'https://gab.com/Flavinkins/posts/108946058120800199 How would you go onto constructing a synthetic consensus backbone that would be 1: guaranteed to be rescuable. 2: easy to manipulate as fragments. 3: easy to manipulate even after being ligated? You would need to choose fragments with type IIS restriction sites such that 1: each individual type IIS site must have a precedent in its presence of absence in the wild in at least 1 Sarbecovirus (distance doesn’t matter here. Can be as distant as BM48-31. Abundance matters statistically (rarer individual sites are less likely to show up at spillover) but does not matter if you are making a clone. As long as you find your desired presence/absence of a site at a specific location in even just one genome, you can use it and be confident that it won’t break your consensus genome. This is governed by both the availability of individual side precedence in sampled genomes and by the location of the site, whatever that doesn’t break the genome and is optimal for cloning gets chosen) to ensure that highly conserved RNA structural motifs aren’t disrupted. 2: there must be no BsaI sites between two BsmBI sites and no BsmBI sites between two BsaI sites to minimize the need of double digestion. 3: the number of overly small fragments should be minimized while no overly large fragments should be present at all. That is, the Standard Deviation (S.D.) Of the length of the fragments shouldn’t be too high. 4: the number of fragments should be kept to as low as possible with the restriction that you can easily manipulate each individual fragment within a M13/pUC vector backbone, as an excess number of fragments are more difficult to keep track of or manipulate. The type IIS restriction sites in the SARS-CoV-2 genome matches all the requirements above. Other genomes in its clade (or even any that is found in Asia)? Not that much. Unfortunately since clonability does not translate to spillover potential (without a lab), the fact that easily clonable Sarbecovirus genomes are rare in general also translates into the possibility that one that ended up spilling over just happen to be one that is easily clonable as SARS-CoV-2, being low. Since the fragments are what that is being chosen for the consensus genome, site selection influences ReCCA especially for the segments that were located near restriction sites—the segments surrounding them are what that were originally chosen in the consensus construct, where one of the requirements used is that the pattern of type IIS sites within the selected set of fragments should make the final genome assembled from them easy to clone. Finally, how could all the non-SARS-CoV-2 genomes you use in the ReCCA graph have a BsaI site in the first position of the F3 fragment (this site is highly conserved in Sarbecoviruses found in Asia) , but the final ReCCA, supposedly generated from related sequences sampled in the wild, not having that site? Either the ReCCA algorithm itself have considered these sites as part of the synonymous sites that was used to infer the “highly conserved segments”, and included SARS-CoV-2 itself into the analysis (making the algorithm tautological), or it is the result of the consensus-generation process where the choice of segments and sites to be used for the consensus included an requirement for the ease of cloning and manipulation for the final genome—something that would matter if you are synthesizing and rescuing it in the lab, not so much for anything that “spills over from the wild”. https://archive.ph/VypuD' gab.com
Flavinkins on Gab: 'In fact, how the ReCCA algorithm functions (it pi…' Flavinkins on Gab: 'In fact, how the ReCCA algorithm functions (it picks the closest sequence to the SARS-CoV-2 branch On “phylogenetic trees constructed on each (very short) segment of the genome”) mean that it is impossible to reconstruct an ReCCA without inputing the SARS-CoV-2 genome into the algorithm (as the reference genome to be aligned against) and thus biasing the result catastrophically—to the point that it is impossible to distinguish this process from what that would be used during the construction of an consensus genome for an infectious clone (fragments are picked with a requirement that the sites on the selected fragments lead to a genome that can be easily synthesized and constructed), and removes any statistical power of “ReCCA” to argue that the specific type IIS restriction pattern of SARS-CoV-2 to be the only possible combination of sites that is “evolutionarily likely”. It once again, failed to provide any biological reason why a specific, <1-in-100, easy-to-clone pattern being any more likely to end up being the spillover strain (that must not use the SARS-CoV-2 genome itself as the reference when such probability is assessed) compared to the >99-in-100 other possible combinations outside the S1 With the “ReCCA components” (where none were easy to clone by themselves) that are not easy to clone, in a non-circular manner. https://gab.com/Flavinkins/posts/109288626916761348' gab.com
Flavinkins on Gab: 'When a synthetic recombinant genome for a coronav…' Flavinkins on Gab: 'When a synthetic recombinant genome for a coronavirus is constructed, fragments are selected from relevant natural bat isolates with a requirement that the type IIS restriction pattern using the planned enzymes on the resulting assembly should enable easy cloning and efficient manipulation—a less than 1 in 100 chance for this to happen randomly by chance for recombination outside the S1 region of the genome, and completely irrelevant to spillover. When a natural virus spills over, there is very little effect (within 1 order of magnitude) on the chance that some specific strain would end up becoming the pandemic strain for recombination ancestry outside the S1 region. There is no requirement that a strain that spills over must be a strain that is easy to clone by the 2 most popular type IIS restriction enzymes that were used in CoV genome assemblies, and the chance given natural spillover of an ancestry that had an efficient type IIS RGS system without modification is the same chance as finding one such strain in nature just by 1 single random sampling—so far no specimen from Asia satisfy this on their individual genomes. Again, which sequence on the ReCCA graph were not sampled from nature? Unfortunately, the so-called “natural recombination ancestry” argument may well just be one of the many ways workable coronavirus genomes are “recovered” from a set of otherwise unisolated samples. Whatever you reconstruct out of natural isolates for a clone, it must be easy to clone. It can be from one of the rare samples you find with an easy-to-clone pattern, or it could be one of the combinations of various contigs from sequencing a pooled sample. It could also be a chimeric genome constructed using fragments selected from related wild isolates with a requirement that the result is easy to clone. When a strain spills over naturally, there is no requirement that it must be easy to clone—restriction enzymes work on DNA not on RNA, and there is no reason why the specific combination with an easy to clone site pattern must be selected other than the posterior claim “it happened” (ReCCA construction used SARS-CoV-2 genome as reference). This is a circular argument as the claim that “the SARS-CoV-2 genome with its unusual combination of type IIS sites is the result of a natural spillover” assumed P(spillover|strain have good site combination)>=0.5 while P(strain have good site combination)<<0.5 with the only justification “we observe that the SARS-CoV-2 genome is easy to clone and can be constructed using a combination of fragments from some 8+ different “bat virus strains”” only able to justify this implied probability assumption with the assumption “SARS-CoV-2 is the result of a natural spillover”, a hypothesis that is being tested in the type IIS RE site analysis paper in stead of an underlying assumption. In conclusion, while a ReCCA with an easy to clone type IIS site combination with the go-to enzymes used for assemblies of this length is a possible combination of the bat coronavirus sequences known from sampling, there is no justification for this hypothetical and still unsampled ReCCA to be the only possible combination where a spillover is possible or that a spillover strain will have a probability that it will be easy to clone being >0.5 while the chance of finding such a strain from a random sampling from the wild being only about ~0.01.' gab.com
Flavinkins on Gab: 'Additional sample formats, such as “bat coronavir…' Flavinkins on Gab: 'Additional sample formats, such as “bat coronavirus isolate NNNNNN” are found in 2015 samples, whereas the 2016 sequences (the last sequences to be deposited into GenBank from known bat coronavirus searching papers in China/CAS) contained within their authors “Edward C Holmes”. This may hint on the role of E.Holmes on the handling of bat Coronavirus sample sequencing for samples collected “between 2015-2019”. Sequences MH315932-MH315944 have formats “XxNNNNNN”, however these contained samples from as early as 2013, making it difficult to ascertain as where these samples came from. However, these numbers (and the “Spread and Geographic Structure of SARS-related Coronaviruses in Bats and the Origin of Human SARS Coronavirus” paper, with E.Holmes in the GenBank records) contained the only deposited CAS samples known to be collected in 2016, which could be taken as evidence of E.Holmes and WIV collaboration for sampling efforts and sequence/sample sharing, in the 2015-2019 period. A collaboration (sample/sequence sharing) between Guangdong and Wuhan institutions through E.Holmes https://zenodo.org/record/6849652#.Y38toiW8klT , can not be ruled out. The original GenBank deposition date of 2018 seems to coincide with the supposed sampling date of “BtSY2”. We now know that some of the samples taken in 2018 under E.Holmes contained RBD sequences related to the Omicron strain of SARS-CoV-2. The question becomes: Why you are taking samples from bats (dissected rectum), without performing some kind of tests immediately after sampling, as early as in 2015-2018, given that an ethics approval for the destructive sampling of wild animals in China required some kind of academic program to be first sent for approval, which have to include the detail of the exact experiments that requires the dissection of the bats and collection of the rectum samples. If any kind of testing/screening/experimentation were done initially on the samples (immediate experimental plan would have to be provided for the sampling proposal to be justified and approved—and “for storage, until some technology that doesn’t yet exist until late 2021” isn’t one of them), what were the results? Why, despite sampling of bats by EHA/CAS and expeditions into Yunnan/Mojiang mine continued all the way until 2019, the last such sequences deposited from China was sampled in 2016? If “This research, including the procedures and protocols of specimen collection and processing, 262 was reviewed and approved by the Medical Ethics Committee of the Yunnan Institute of 263 Endemic Diseases Control and Prevention. (No. 20160002).”, why weren’t the results immediately made available to the public as the samples were collected and processed over the course of 4 years? RNA samples are after all, very fragile and does not tolerate long-term storage very well. When were these actually sequenced? (I see 15 different sequencing machines in the FCIDs, with FCIDs ranging from H2 to H7, HG to HY, and sequencing on the same machines hundreds of runs apart. This could indicate sequencing done immediately after sampling) And if these were sequenced before the pandemic, who had access to the sequences? Were these really “newly discovered” viruses, or just sequences that were put into embargo in the “great silence” of CAS accession numbers between 2016-2019, only recently released? (Should these samples be sequenced pre-pandemic, near their collection date in 2018, this could be corresponding to phase 1(QS0) of DEFUSE. After sequences generated as early as in mid-2018, there would be more than 1 year to work with the cloning and culture experiments.) The machines found in https://github.com/Augustpan/Individual-Bat-Virome/blob/main/raw_data/lane_id_table.csv Are: A01426 A00821 A00920 A00270 A00877 A00289 A00881 A00783 A00253 A00917 A01045 A00808 A00459 A01415 A01050 The samples with BtSY2 are: S18CXBatR24 @A00917:648:H3Y25DSX2:4 S18CXBatR29 @A00783:739:H3V32DSX2:3 Both appeared to be on the earliest run on that particular machine, where the run contained no sample from 2019, and where the flow cell ID were from an early era (H3). Archive for read mappings: https://archive.ph/CuzzR Archive for lane IDs: https://archive.ph/IKxD1' gab.com
Flavinkins on Gab: 'To see more about the DOD-sponsored Institut Past…' Flavinkins on Gab: 'To see more about the DOD-sponsored Institut Pasteur sampling of the BANAL caves in 2017 and the censorship of even the already-sequenced batflies library https://gab.com/Flavinkins/posts/109150240613656613 https://gab.com/Flavinkins/posts/109139121799069783 https://gab.com/Flavinkins/posts/108782000872829271 http://gab.com/Flavinkins/posts/108745003276913992 https://gab.com/Flavinkins/posts/108938621623162894 https://gab.com/Flavinkins/posts/108961381086722860 https://gab.com/Flavinkins/posts/108808844066927838 https://gab.com/Flavinkins/posts/109198525541365424 https://gab.com/Flavinkins/posts/109222447665307488 PREDICT-2 and EHA activity in SE. Asia including Southern Laos https://gab.com/Flavinkins/posts/109079936391006382 https://gab.com/Flavinkins/posts/109079963106312117 Past lab escapes from IP france https://gab.com/Flavinkins/posts/108929427082821215 And on the type IIS REase found in IP cambodge https://gab.com/Flavinkins/posts/109215673255176764 https://gab.com/Flavinkins/posts/109216529429569399' gab.com
Flavinkins on Gab: '@Graviola_Finland @EIGARBARINO https://archive.m…' Flavinkins on Gab: '@Graviola_Finland @EIGARBARINO https://archive.md/8rlbT FULL TRANSLATED TEXT of the briefing of the Ministry of Defense of the Russian Federation on the analysis of documents related to military biological activities of the United States, made on January 30, 2023 with the supporting documentation that it provided. https://gab.com/Flavinkins/posts/108782801366501491 https://gab.com/Flavinkins/posts/108908816879287433 https://gab.com/Flavinkins/posts/108808844066927838 https://gab.com/Flavinkins/posts/108808523459345532 It confirms that sampling and researching effort for S.E. Asia is in deed being conducted in these labs. Anomalies regarding the Caspian sea in 2019: https://gab.com/Flavinkins/posts/108949034317465342 EHA activity in the Caspian sea region, up to 2019: https://gab.com/Flavinkins/posts/108782587895528182 https://gab.com/Flavinkins/posts/109139121799069783 https://gab.com/Flavinkins/posts/109529211058973737 DOD, IP laos and batflies🦇🪰: https://gab.com/Flavinkins/posts/108961381086722860 https://gab.com/Flavinkins/posts/108938621623162894 https://gab.com/Flavinkins/posts/108782000872829271 https://archive.md/JZkwi EHA, Laos and PREDICT-2: https://gab.com/Flavinkins/posts/109158749548555994 https://gab.com/Flavinkins/posts/109079936391006382 https://gab.com/Flavinkins/posts/109079963106312117 https://archive.md/hMp7x 🦇🧪🥣? https://gab.com/Flavinkins/posts/109150240613656613 https://gab.com/Flavinkins/posts/109198525541365424 https://gab.com/Flavinkins/posts/109222447665307488 https://gab.com/Flavinkins/posts/109728264957247673 https://archive.ph/3fFfn https://archive.md/T6sN5 (No wildlife trade route linking Wuhan to Laos…… But plenty of sampling route linking Laos to labs both in Wuhan and on the East Coast……)' gab.com

@NestCommander - Kevin W. McCairn PhD

Before they begun enforcing their claim of “100/174 centered around the market” and starting to tamper with data to make the claim, https://ghrp.biomedcentral.com/articles/10.1186/s41256-021-00200-8 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149375/ 135/92 and 115/82 cases already got into in early peer-reviewed papers that went missing in the WHO report. Past media reports archive.md/Ea0Kw archive.md/1x658 also contradict WHO in key early cases’ residences, including the earliest case they admit in the WHO report. archive.md/5sdkR archive.md/1pcCU archive.md/N0hib archive.md/VXtu9 archive.is/Kyr1z https://archive.org/details/mace-e-pai-covid-19-analysis-redacted/page/8/mode/1up And you know that they hate this information when it was censored. The MACE-EPAI document here is not searchable on google. Up to one third of all cases were either removed completely or moved toward the market in the “dataset”. archive.md/zUD1F archive.md/Pc6gp https://archive.is/p3K3Z Including the very first case they ever admitted officially. And outright removed 4 times more cases than official. Unlinked cases supposedly secondary to linked cases should cluster around them, not the market itself. archive.md/GvRcD archive.md/ZgVzp Wuhan authorities after that archive.md/OIGPz 2014 incident now targeted only the Huanan market when looking for EID outbreaks—and nowhere else. archive.md/1x658 They tampered with the early cases data archive.md/Ea0Kw To make it look like it “started at the market” when in reality the first case they ever admitted lived right next to the WIV BSL-4. archive.md/5sdkR severe discrepancy happening December 2019 and January 2020 indicate tampering with case counts. archive.md/1pcCU This is indicative of catastrophic ascertainment bias was going on. None of China’s “early cases” dataset is credible. https://archive.md/ET1GA https://archive.md/Ea0Kw https://archive.md/1x658 The tampering of early case residence data is systematic and extensive. It is the reason why they refused to provide this data in any detail at all. Not only did The first every case they admitted live in Shidong right next to the BSL-4, and were moved toward the market in the WHO report in contradiction to all known media coverage, https://gab.com/Flavinkins/posts/109256201942085712 the entirety of Wuchang district was wiped clean for every single WHO case that have onset before 27/12/2019–with up to 3000 cases moved to the market this way over the entire Wuhan outbreak. https://archive.md/1x658 and for central Wuchang near the labs and the densest inhabited regions inside the district, all cases were moved away in the WHO map. Unfortunately Rasmussen's work on the origins question rests heavily on what David Relman described as "hopelessly impoverished" early case data. https://www.washingtonpost.com/national-security/2023/02/27/little-known-scientific-team-behind-new-assessment-covid-19-origins/ https://www.washingtonpost.com/opinions/2022/11/17/covid-early-cases-wuhan-china-mystery/ https://archive.md/ke1lp https://archive.md/RaYPC David Fisman: I think the most interesting thing this fellow says is that there are clearly tens of thousands of cases...That implies a much earlier introduction than would have occurred with a seafood market outbreak..."

The comparison of epidemiological characteristics between confirmed and clinically diagnosed cases with COVID-19 during the early epidemic in Wuhan, China - Global Health Research and Policy To put COVID-19 patients into hospital timely, the clinical diagnosis had been implemented in Wuhan in the early epidemic. Here we compared the epidemiological characteristics of laboratory-confirmed and clinically diagnosed cases with COVID-19 in Wuhan. Demographics, case severity and outcomes of 29,886 confirmed cases and 21,960 clinically diagnosed cases reported between December 2019 and February 24, 2020, were compared. The risk factors were estimated, and the effective reproduction number (Rt) of SARS-CoV-2 was also calculated. The age and occupation distribution of confirmed cases and clinically diagnosed cases were consistent, and their sex ratio were 1.0 and 0.9, respectively. The epidemic curve of clinical diagnosis cases was similar to that of confirmed cases, and the city centers had more cumulative cases and higher incidence density than suburbs in both of two groups. The proportion of severe and critical cases (21.5 % vs. 14.0 %, P < 0.0001) and case fatality rates (5.2 % vs. 1.2 %, P < 0.0001) of confirmed cases were all higher than those of clinically diagnosed cases. Risk factors for death we observed in both of two groups were older age, male, severe or critical cases. Rt showed the same trend in two groups, it dropped below 1.0 on February 6 among confirmed cases, and February 8 among clinically diagnosed cases. The demographic characteristics and spatiotemporal distributions of confirmed and clinically diagnosed cases are roughly similar, but the disease severity and clinical outcome of clinically diagnosed cases are better than those of confirmed cases. In cases when detection kits are insufficient during the early epidemic, the implementation of clinical diagnosis is necessary and effective. ghrp.biomedcentral.com
Association of Public Health Interventions With the Epidemiology of the COVID-19 Outbreak in Wuhan, China Was there an association of public health interventions with improved control of the COVID-19 outbreak in Wuhan, China?In this cohort study that included 32 583 patients with laboratory-confirmed COVID-19 in Wuhan from December 8, 2019, through ... ncbi.nlm.nih.gov
MACE E PAI COVID 19 ANALYSIS Redacted : Free Download, Borrow, and Streaming : Internet Archive MACE E-PAI COVID-19 ANALYSIS archive.org
Opinion | Wuhan’s early covid cases are a mystery. What is China hiding? The story of how the pandemic got started — and turned into a global catastrophe — remains a black box. It should not be. washingtonpost.com

@NestCommander - Kevin W. McCairn PhD

https://www.nytimes.com/2021/02/12/world/asia/china-world-health-organization-coronavirus.html https://archive.md/UFrSv They systematically moved more than 3000 cases from the lab to the market and gave “cases data” that they wanted to push for market as first outbreak site to distance from the labs. https://www.researchgate.net/publication/370635299_Greater_than_the_Sum_of_its_Parts_-_Aggregated_Wuhan_COVID-19_case_data_points_to_the_wrong_side_of_the_Yangtze_River_-_Rixey_-_20230509 Such an result of having unlinked cases closer to the market than linked cases is not expected even under the null hypothesis of market origin, which we should see unlinked cases secondary to and cluster around the linked cases, and not the market itself. https://www.researchgate.net/publication/370635299_Greater_than_the_Sum_of_its_Parts_-_Aggregated_Wuhan_COVID-19_case_data_points_to_the_wrong_side_of_the_Yangtze_River_-_Rixey_-_20230509 Not only there were an complete absence of verifiability in Chinese cases, there is direct non-circumstantial evidence that they moved up to 3000 cases from Wuchang to Huanan. In fact, it is totally not normal to have unlinked cases closer to the market than linked cases—the only way this can happen is with ascertainment bias. Only near the market gets ascertained if not directly linked to it. Base rate neglect. They did the exact same thing when claiming that all 67 “pre-Huanan checkable cases” were “serologically negative”. Again, the social media associated here say “before Jan 18, 2020”. Included all Dec cases. https://www.mdpi.com/2220-9964/9/6/402 It is actually impossible for unlinked cases, supposedly secondary, to cluster closer to the market than linked cases which supposedly to be primary, without significant sampling bias or outright manipulation in the underlying “data”. Both evidently happened. https://arxiv.org/pdf/2401.08680.pdf https://archive.md/JVFuc If you toss away anything that is not officially announced by China in bold, then obviously you would arrive at exactly what China wanted you to believe.

On W.H.O. Trip, China Refused to Hand Over Important Data (Published 2021) The information could be key to determining how and when the outbreak started, and to learning how to prevent future pandemics. nytimes.com
ResearchGate - Temporarily Unavailable researchgate.net
ResearchGate - Temporarily Unavailable researchgate.net
Exploring Urban Spatial Features of COVID-19 Transmission in Wuhan Based on Social Media Data During the early stage of the COVID-19 outbreak in Wuhan, there was a short run of medical resources, and Sina Weibo, a social media platform in China, built a channel for novel coronavirus pneumonia patients to seek help. Based on the geo-tagging Sina Weibo data from February 3rd to 12th, 2020, this paper analyzes the spatiotemporal distribution of COVID-19 cases in the main urban area of Wuhan and explores the urban spatial features of COVID-19 transmission in Wuhan. The results show that the elderly population accounts for more than half of the total number of Weibo help seekers, and a close correlation between them has also been found in terms of spatial distribution features, which confirms that the elderly population is the group of high-risk and high-prevalence in the COVID-19 outbreak, needing more attention of public health and epidemic prevention policies. On the other hand, the early transmission of COVID-19 in Wuhan could be divide into three phrases: Scattered infection, community spread, and full-scale outbreak. This paper can help to understand the spatial transmission of COVID-19 in Wuhan, so as to propose an effective public health preventive strategy for urban space optimization. mdpi.com

@blink64 - polyploidy

When actual statisticians have a go at your paper purporting to show the SARS2 outbreak originates at the HSM. Thanks @gdemaneuf! https://t.co/avfBP4bZPa

@NestCommander - Kevin W. McCairn PhD

@threadreaderapp unroll

Saved - March 7, 2025 at 5:19 PM
reSee.it AI Summary
I believe there is a deliberate cover-up regarding the origins of COVID-19, particularly concerning the Wuhan P4 lab. The narrative that the virus originated from the Huanan market is questionable, especially given the absence of positive animal samples and secondary outbreaks. Data manipulation appears evident, with early case counts altered to support the market theory. Despite extensive wildlife sampling, results consistently returned negative, and attempts to blame smuggled animals or frozen food seem to be a diversion. The focus on the Huanan market raises concerns about obscuring potential lab-related sources.

@KevinMcCairnPhD - Kevin W. McCairn PhD

https://x.com/nestcommander/status/1781826378683556254?s=46&t=wRQSWp_1VffWmS2vKQwhSA… When they begun an official censorship campaign on all and anything on the “Wuhan P4 lab”, @AP you know that they are acting out a pre-scripted cover-up effort, and was not “genuingly clueless”. Especially when the expected precaution in response to an ongoing outbreak, such as washing hands, was also intentionally avoided. https://x.com/daoyu15/status/1718782597114016231?s=46&t=wRQSWp_1VffWmS2vKQwhSA… The entirety of the “they covered up the market” theory can also be interpreted as that they don’t want to let the NEGATIVE animal samples from being known. They need to shroud it in mystery, or the https://x.com/daoyu15/status/daoyu15/status/1694163822473629792?s=46&t=wRQSWp_1VffWmS2vKQwhSA… absence of any secondary spillovers in any other markets in China https://x.com/nestcommander/status/1779485262005023009?s=46&t=wRQSWp_1VffWmS2vKQwhSA…, when SARS1 have already did 7 times over the same month and a half of no actions https://x.com/daoyu15/status/daoyu15/status/1687891376665681920, would be confirmed in the total absence of any positive animal swabs at the market. https://x.com/daoyu15/status/daoyu15/status/1754661054733242856 China, as in the national level, performed sampling of the entirety of the wildlife supply chain toward the market and have officially insisted that “it came from illegal wildlife sold at the Huanan market” up to May 2020. They even tried to blame pangolins, among the others. All the sampling results are negative, and which are in fact leaked even during this period of “you can blame only the animals”. https://x.com/daoyu15/status/daoyu15/status/1668828125617352704?s=46&t=wRQSWp_1VffWmS2vKQwhSA… And once again, a lower down that was tasked to eliminate potential negative evidence by the higher up, but only told to eliminate “potential evidence” in general, can easily come up with conspiracy theories about “they are tasking to eliminate positive evidence”; https://x.com/nestcommander/status/1775081708007878978?s=46&t=wRQSWp_1VffWmS2vKQwhSA… When in fact, The fear that a thorough investigation would turn up confirming all animals being negative and that their labs would become blamed immediately, being the the real cause, is evident in both the absence of spillovers anywhere alongside or in any other destinations of the wildlife supply chain, and the leaked preliminary sampling efforts among these supply chains that returned no positivity at all, https://x.com/daoyu15/status/1740641874032185732?s=46&t=wRQSWp_1VffWmS2vKQwhSA… Despite at the time when “wild animals illegally traded at the Huanan market” and then “wild animals” was the only permitted origin theory on all official outlets in China (up to May 2020).

@KevinMcCairnPhD - Kevin W. McCairn PhD

When they begun an official censorship campaign on all and anything on the “Wuhan P4 lab”, you know that they are acting out a pre-scripted cover-up effort, and was not “genuingly clueless”. Especially when the expected precaution in response to an ongoing outbreak, such as washing hands, was also intentionally avoided.

@KevinMcCairnPhD - Kevin W. McCairn PhD

And of course, they also attempted to blame it on a smuggled animal, or frozen food, as the negativity of all wildlife sampling (which is already known in Jan-Feb 2020 with the data leaks archive.md/iw1Pz archive.md/4rVph on failing to get positive results even in the upstream supply farms of the market) in China is confirmed by total absence of secondary outbreaks or secondary spillovers in China when raccoon dogs have already been made livestock and wildlife trade being still alive and well online. Their evident tampering of the early cases databases And their blatant and statistically unsound lies over the serology of all the cases before the market outbreak (which none are linked to any wildlife markets at all) Confirmed that their agenda is to push the outbreak onto the market nomatter what source they have to blame on, even if they have to disclose the negativity of tests as leaked before, they need to find an explanation as “frozen food or smuggled animal”.

@KevinMcCairnPhD - Kevin W. McCairn PhD

Before they begun enforcing their claim of “100/174 centered around the market” and starting to tamper with data to make the claim, https://ghrp.biomedcentral.com/articles/10.1186/s41256-021-00200-8 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149375/ 135/92 and 115/82 cases already got into in early peer-reviewed papers that went missing in the WHO report. Past media reports archive.md/Ea0Kw archive.md/1x658 also contradict WHO in key early cases’ residences, including the earliest case they admit in the WHO report. archive.md/5sdkR archive.md/1pcCU archive.md/N0hib archive.md/VXtu9 archive.is/Kyr1z https://archive.org/details/mace-e-pai-covid-19-analysis-redacted/page/8/mode/1up And you know that they hate this information when it was censored. The MACE-EPAI document here is not searchable on google. Up to one third of all cases were either removed completely or moved toward the market in the “dataset”. archive.md/zUD1F archive.md/Pc6gp https://archive.is/p3K3Z Including the very first case they ever admitted officially. And outright removed 4 times more cases than official. Unlinked cases supposedly secondary to linked cases should cluster around them, not the market itself. archive.md/GvRcD archive.md/ZgVzp Wuhan authorities after that archive.md/OIGPz 2014 incident now targeted only the Huanan market when looking for EID outbreaks—and nowhere else. archive.md/1x658 They tampered with the early cases data archive.md/Ea0Kw To make it look like it “started at the market” when in reality the first case they ever admitted lived right next to the WIV BSL-4. archive.md/5sdkR severe discrepancy happening December 2019 and January 2020 indicate tampering with case counts. archive.md/1pcCU This is indicative of catastrophic ascertainment bias was going on. None of China’s “early cases” dataset is credible. https://archive.md/ET1GA https://archive.md/Ea0Kw https://archive.md/1x658 The tampering of early case residence data is systematic and extensive. It is the reason why they refused to provide this data in any detail at all. Not only did The first every case they admitted live in Shidong right next to the BSL-4, and were moved toward the market in the WHO report in contradiction to all known media coverage, https://gab.com/Flavinkins/posts/109256201942085712 the entirety of Wuchang district was wiped clean for every single WHO case that have onset before 27/12/2019–with up to 3000 cases moved to the market this way over the entire Wuhan outbreak. https://archive.md/1x658 and for central Wuchang near the labs and the densest inhabited regions inside the district, all cases were moved away in the WHO map. Unfortunately Rasmussen's work on the origins question rests heavily on what David Relman described as "hopelessly impoverished" early case data. https://www.washingtonpost.com/national-security/2023/02/27/little-known-scientific-team-behind-new-assessment-covid-19-origins/ https://www.washingtonpost.com/opinions/2022/11/17/covid-early-cases-wuhan-china-mystery/ https://archive.md/ke1lp https://archive.md/RaYPC David Fisman: I think the most interesting thing this fellow says is that there are clearly tens of thousands of cases...That implies a much earlier introduction than would have occurred with a seafood market outbreak..."

The comparison of epidemiological characteristics between confirmed and clinically diagnosed cases with COVID-19 during the early epidemic in Wuhan, China - Global Health Research and Policy To put COVID-19 patients into hospital timely, the clinical diagnosis had been implemented in Wuhan in the early epidemic. Here we compared the epidemiological characteristics of laboratory-confirmed and clinically diagnosed cases with COVID-19 in Wuhan. Demographics, case severity and outcomes of 29,886 confirmed cases and 21,960 clinically diagnosed cases reported between December 2019 and February 24, 2020, were compared. The risk factors were estimated, and the effective reproduction number (Rt) of SARS-CoV-2 was also calculated. The age and occupation distribution of confirmed cases and clinically diagnosed cases were consistent, and their sex ratio were 1.0 and 0.9, respectively. The epidemic curve of clinical diagnosis cases was similar to that of confirmed cases, and the city centers had more cumulative cases and higher incidence density than suburbs in both of two groups. The proportion of severe and critical cases (21.5 % vs. 14.0 %, P < 0.0001) and case fatality rates (5.2 % vs. 1.2 %, P < 0.0001) of confirmed cases were all higher than those of clinically diagnosed cases. Risk factors for death we observed in both of two groups were older age, male, severe or critical cases. Rt showed the same trend in two groups, it dropped below 1.0 on February 6 among confirmed cases, and February 8 among clinically diagnosed cases. The demographic characteristics and spatiotemporal distributions of confirmed and clinically diagnosed cases are roughly similar, but the disease severity and clinical outcome of clinically diagnosed cases are better than those of confirmed cases. In cases when detection kits are insufficient during the early epidemic, the implementation of clinical diagnosis is necessary and effective. ghrp.biomedcentral.com
Association of Public Health Interventions With the Epidemiology of the COVID-19 Outbreak in Wuhan, China Was there an association of public health interventions with improved control of the COVID-19 outbreak in Wuhan, China?In this cohort study that included 32 583 patients with laboratory-confirmed COVID-19 in Wuhan from December 8, 2019, through ... ncbi.nlm.nih.gov
MACE E PAI COVID 19 ANALYSIS Redacted : Free Download, Borrow, and Streaming : Internet Archive MACE E-PAI COVID-19 ANALYSIS archive.org
Flavinkins on Gab: 'https://gab.com/Flavinkins/posts/1088805315972559…' Flavinkins on Gab: 'https://gab.com/Flavinkins/posts/108880531597255968 https://gab.com/Flavinkins/posts/109147956977669077 Also, remember accountant Chen? (Why his dot was moved to the WCH if he lives in Wuchang/Jiangxia?) it turned out that it was not only his dot that was not in the right place. Every dot within the 2km radius of the Wuchang railway station was moved or removed. This is within the area that is expected to have the infectious disease and respiratory cases ultimately serviced by the “中部战区总医院”. The hospital that sees large-scale respiratory case anomalies the first in Wuhan on official records, where the decision to “enter battle stations” on 01/01/2020 was made because of an “unexpected and fast-growing anomaly in the respiratory disease surveillance data” beginning at least as late as 31/12/2019. There were dots that were east of this area, and there were dots that were south of this area, in locations with lower population density compared to downtown Wuchang and further from the market. (2 ambulances from Jiangxia on 31/12/2019, but only 1 dot on the WHO map and he wasn’t accountant chen…… (likely ascertained by contacting a HSM case on public transport, “试行诊疗方案”) (accountant Chen got to the WCH in 27/12/2019)) Considering how cases that were admitted to the “中部战区总医院” weren’t directly reported except for WH01 in 14/01/2020, (Only 1 out of the 4 known sequenced cases here were directly reported. WH03 is reported after transfer to the Zhongnan hospital, one of the 2 initial market cases reported in the location. WH02 and WH04 were not in the NNDRS dataset and displayed as “unknown” in the WHO report) and how they then report seeing more fever cases in a single day than the entire CDC pre-04/01/2020 onset dataset (the point when they have to expand their fever clinics), it is quite likely that cases that initially broke out in Wuchang were muted by admission into a hospital that is placed under a command that doesn’t have to report on the NNDRS, and that any cases found in Downtown Wuchang had their “residential addresses” altered to place them as close to the Huanan market as possible and out of the Wuchang area. This would not be the first time when cases that came from an “inconvenient” location were hidden inside PLA-operated hospitals to prevent them from being counted. https://gab.com/Flavinkins/posts/109701931477090563 Why the WMHC rejected the WHO’s demand for line listings of the 174 “NNDRS cases” in annex E2? Also, one dot in Jiangxia is one of the two https://gab.com/Flavinkins/posts/109048819612838694 ambulances that were seen in 31/12/2019 from Jiangxia. Only one become a dot (central Jiangxia as opposed to the Shidong prefecture). It is possible that this is Chen’s relative that “visited a local market”, meaning that this is a case that is ascertained by contact with an early case, and saved from removal because of post-27/12/2019 onset. No dot at all is inside the borders of the WuChang district, even when dots begin showing up east of it in less populated places further from the market. This is clearly artefactual, indicating attempt at breaking up the cluster in Wuchang.' gab.com
Little-known scientific team behind new assessment on covid-19 origins A small shift in favor of the “lab leak” theory was prompted by new data and an A-list team of weapons-lab scientists. washingtonpost.com
Opinion | Wuhan’s early covid cases are a mystery. What is China hiding? The story of how the pandemic got started — and turned into a global catastrophe — remains a black box. It should not be. washingtonpost.com

@KevinMcCairnPhD - Kevin W. McCairn PhD

https://archive.md/UIBkB https://archive.md/6LuXg https://web.archive.org/web/20231101133202/https://en.rattibha.com/thread/1718570491534061745 archive.md/ZgVzp https://archive.md/fWTg1 The Huanan market was the only place in Wuhan monitored for EID since 2014. This is to ensure that whenever an outbreak occurs the only place it will be first detected would be at the Huanan market itself, ensuring that all lab escapes can be covered up and scapegoat campaigns can initiate to blame the wildlife trade in stead. The official narrative is always that “it was caused by wild animals sold in the Huanan market” and “It have an origin within illegally traded wild animals” all the way up to May 2020–the pub date for the Pangolin papers is up to April 2020 and that a bounty to “find the animal origins” for SARS-CoV-2 is still active in May 2020. The “most likely origin being wild animals” argument is present in Chinese articles about SARS-CoV-2 all over 2020, where numerous attempts at finding animal hosts were performed in-vitro but always land onto Homo Sapiens and leave their “primary suspects” otherwise being animals not sold at the Huanan market, to much of the dismay of the authors, despite the themes being almost always “there is a broad host range for SARS-CoV-2” to try stretch the search efforts as wide as possible, as long as possible, bidding to keep up with the increasingly longer list of negative farms and species in the national search efforts. Despite their numerous attempts at removing the human correlational edge with SARS-CoV-2, their effort ultimately failed with either the proportionality and thus the unique positive correlation and mutual information between Homo Sapiens and SARS-CoV-2 is preserved, or with all of the mutual information between SARS-CoV-2 and any land-dwelling species at all being destroyed.

@KevinMcCairnPhD - Kevin W. McCairn PhD

https://michaelweissman.substack.com/p/an-inconvenient-probability-v57 The meter-precise market centered KDE of W+P “unlinked cases” required in addition to unreasonable and knowingly false assumptions on the social contact and shared space structures of market cases including visitors especially when they occur outside the market, an total absence of anisotropies and biases within the populational and movement structures around the market, neither of which are proven, and quite the opposite—an extreme level of anisotropy, again biasing toward where the linked cases are found, are observed. Yet somehow the unlinked cases were ~50m farther away in opposite to these bias directions when compared to the market itself, which once again required precise recentering of “data” by manipulative actions. (edited) An Inconvenient Probability v5.7 Bayesian analysis of the probable origins of Covid. Quantifying "friggin' likely"

An Inconvenient Probability v5.11 Bayesian analysis of the probable origins of Covid. Quantifying "friggin' likely" michaelweissman.substack.com

@KevinMcCairnPhD - Kevin W. McCairn PhD

To the earliest Wuhan authorities that prioritize “blame the animals” to prevent scrutiny to the lab (which they also happen to initiate before any public inquiries on this matter could even begin), “no swabs” are better than “negative swabs”. Sadly some of these animal swabs do got taken away at that time just because “gather relevant samples as close as possible and then inspect them” being one of the standard procedures for many of the agencies-of-interest outside Wuhan, and when they are examined, again, per standard procedures, the results got leaked in January 2020, and all of which are negative.

Saved - July 2, 2024 at 3:21 PM
reSee.it AI Summary
The posts discuss the challenges of cultivating live zoonotic viruses from fecal samples, the possibility of synthetic chimeras, and the use of intracerebral inoculation to infect lab animals. There is speculation about the origin of certain viruses and the potential involvement of specific institutions. The posts also mention the distribution of pangolin samples and the lack of independent detection of pangolin viruses. The author hints at a forthcoming thread on "Getting away with it" and teases a future post on airborne respiratory viruses.

@breakfast_dogs - Dog's Breakfast

How to Make and Deploy a Bioweapon and Get Away With It. Don't try this at home (unless your home is at least BSL-3) Part 1: Growing a Backbone Cultivating a live zoonotic virus from a sample is much harder than it is to sequence one from RNA fragments. But there are ways. 🧵 https://t.co/OwdEG5nBVs

@breakfast_dogs - Dog's Breakfast

WIV claim to have only ever obtained 3 live bat cov isolates: WIV1, WIV16 & RsSHC014. but I'm not convinced these are from natural samples at all, I suspect they're synthetic chimeras. WIV claim they cultivated these from bat fecal samples, but this is particularly challenging.

@breakfast_dogs - Dog's Breakfast

Viruses can't survive for long without host cells to infect and replicate, so feces must be very fresh (or quickly frozen). Even then, bat feces contain many microorganisms (yeasts/fungi, bacteria, phages), and cytotoxic substances that can kill cells before they can be infected. https://t.co/B7WSKMmdaT

@breakfast_dogs - Dog's Breakfast

WIV, and CSIRO "batman" Linfa Wang unsuccessfully tried to rescue viruses from feces several times. AMMS also tried and failed. No-one had successfully recovered any live RNA virus from feces before WIV1was announced in 2013. https://t.co/pgQWEByRGi

@breakfast_dogs - Dog's Breakfast

To achieve this, WIV did little differently than on previous attempts. Apparently they got lucky. Doubly so because they managed to find the precursor to SARS. But they had also been quietly trying to engineer this hypothesized "missing link" virus in the lab. So, I wonder...? https://t.co/aavUoBJgF6

@breakfast_dogs - Dog's Breakfast

A Baric led UNC/Vanderbilt team had previously rescued an artificial chimeric bat virus with the RBD from human SARS substituted. A synthetic virus avoids the problems of toxic contaminants and keeping the virus alive. But Baric was still unable to cultivate a purely bat virus. https://t.co/WVndXxKYBm

@breakfast_dogs - Dog's Breakfast

WIV also failed at this apparently (at least initially). https://t.co/2rAAIq8fuZ

@breakfast_dogs - Dog's Breakfast

But if obtaining live virus from feces and cell culture is hard (or impossible), there is a simpler way if you don't care so much about maintaining fidelity with the original strain. And it also has other advantages for those interested in infecting different host species... https://t.co/GvHKSCKY3R

@breakfast_dogs - Dog's Breakfast

Intracerebral inoculation is the technique the PLA used in 2017 to infect suckling immune deficient mice with their bat virus discovery ZC45. It involves directly injecting fluid from ground infected tissue of freshly killed animals directly into the brains of live lab animals. https://t.co/oFj7z27OqA

@breakfast_dogs - Dog's Breakfast

This technique isn't new. It was used as far back as 1930 to infect mice with yellow fever. Normally the brain is protected from infection by the blood-brain barrier. The technique circumvents that defense, and the immune response in the central nervous system is far less robust. https://t.co/0lWlTH8xPU

@breakfast_dogs - Dog's Breakfast

Immune deficient mice aren't the only option for applying this technique. In the 1990's Baric was developing theories of coronavirus spillover. On several occasions he refers to an experiment involving the intracerebral inoculation of primates with mouse coronavirus MHV. https://t.co/ui5RLWMYcu

@breakfast_dogs - Dog's Breakfast

Another Baric insight was that persistent infection (up to 210 days) produced MHV variants which could infect cells from other species. Using immune-suppressed baby mice or cell culture allows infection to persist, rather than be cleared by the immune system in days, as normal. https://t.co/6n0qmbxKvz

@breakfast_dogs - Dog's Breakfast

We know the PLA used this technique with the ZC45 virus and BALB/C mice, might they have also used it on other species? An October 2016 paper made some interesting observations about the pangolin immune system (h/t @Engineer2The) suggesting a new immune deficient animal model. https://t.co/og4zVtcEvc

@breakfast_dogs - Dog's Breakfast

By the next year Yigang Tong of AMMS was working (in secret) on purported pangolin viruses. Coincidence? https://t.co/0E5X7SsrWe

@breakfast_dogs - Dog's Breakfast

The pangolin samples from AMMS have been distributed far and wide. I know of five separate groups (WIV, HKU, GIABR, SCAU, NUS) that published papers about them, though none acknowledge the PLA source. They were instructed to say they came from 某些特定机构 "certain institutions".

@breakfast_dogs - Dog's Breakfast

Curiously, others have been unable to independently detect pangolin viruses. Some unusually useful work from EcoHealth produced this null result👇 https://t.co/Hlr6uYPBRv

@breakfast_dogs - Dog's Breakfast

But it's also possible the pangolin was only a convenient scapegoat, never infected in nature or a lab, but samples of pangolin tissue deliberately contaminated to give that impression. I'll weigh the evidence for this in a forthcoming thread on "Getting away with it".

@breakfast_dogs - Dog's Breakfast

Some handy hints for the DIY-er if you really insist on trying this out at home. At least make sure an exhaust fan is running and wear some rubber gloves. This is the level (BSL-2) that Yigang Tong worked on pangolin viruses. https://t.co/RfCcGKZxgi

@breakfast_dogs - Dog's Breakfast

Thanks to @drlimengyan1 @Engineer2The @NLink247 @daoyu15 for ideas and discussions on this topic. Coming soon... Part 2: "Give your pathogen wings: what makes an airborne respiratory virus?"

Saved - December 3, 2024 at 9:33 PM
reSee.it AI Summary
Since late August 2024, both China and the West have developed AI methods to manipulate scientific data, particularly using generative adversarial networks. This raises concerns about the reliability of data from conflicted virologists. The true infectivity of SARS-CoV-2 is narrower than claimed, as many wildlife species have not been infected despite overlapping habitats with certain bats. Science is vulnerable to data tampering, especially by authoritarian regimes, leading to inconsistencies that undermine the validity of datasets. Only Homo Sapiens should be trusted in future posts from these virologists.

@NestCommander - Kevin W. McCairn PhD

https://sciencedirect.com/science/article/pii/S2666389922001039 @COVIDSelect @Rebecca21951651 @ban_epp_gofroc @BiophysicsFL @DrLiMengYAN1 @lude_media @FBI @mattwridley @msabouri @LawrenceSellin @WashburneAlex @quay_dr @BillyBostickson Just remember: since about late August 2024, both China and the west have completed the construction of AI based methodologies related to generative adversarial networks(GANs) to falsify scientific data. (And obviously you can use transcriptome mapping methods to carve animal and viral transcriptomes into fraudulent “samples” before merging them.) We should not trust any of the “data” from conflicted virologists in any way now.

AI-enabled image fraud in scientific publications Destroying image integrity in scientific papers may result in serious consequences. Inappropriate duplication and fabrication of images are two common… sciencedirect.com

@NestCommander - Kevin W. McCairn PhD

And of course. The full native gene and specific conditions inside an animal is what that matters for infectivity. https://docs.google.com/document/d/1HeZdCnDA4WpoO_kzISjmoFzIAqgFha3fNEPvBpp2z3M/edit https://docs.google.com/document/d/18d_IMZU_DYRX1DlXuSNySC_4DiTcgabjhxoz9K4tWrg/edit That is why in practice the actual observed host range of SARS-CoV-2 is much, much narrower than the claims of “broad host tropism”, and which all old world wildlife species when their native habitats overlap with that of a rhinolophus bat that carries an ACE2-using Sarbecovirus, failed to become infected in nature by any virus that carried the SARS-CoV-2 RBD including SARS-CoV-2 itself.

Page Not Found Web word processing, presentations and spreadsheets docs.google.com
Page Not Found Web word processing, presentations and spreadsheets docs.google.com

@NestCommander - Kevin W. McCairn PhD

https://t.co/3rrwEoR08a “science” is extremely susceptible to the tampering and poisoning of the underlying “data” by authoritarian regimes. Including with inconsistencies and artifacts that forensically invalidates the “datasets”. And of course, methodologies for data fraud especially from the CCP have obviously kept improving over time.

@NestCommander - Kevin W. McCairn PhD

Only the Homo Sapiens would be real in any newer posts from these conflicted “virologists”. https://t.co/TF5dZbUWLE

Saved - December 20, 2024 at 8:24 PM
reSee.it AI Summary
I have uncovered significant findings regarding the Pangolin Riddle. Evidence confirms that the Wuhan Institute of Virology had pangolins in 2019 and isolated MERS-like coronaviruses from them just before the pandemic. Research indicates that a specific pangolin virus, MjHKU4r-CoV-1, possesses a furin cleavage site that enhances its ability to infect human cells. This suggests a possible link between lab experiments and the emergence of SARS-CoV-2, raising questions about the pathways that led to its acquisition of this feature.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

🧵📸 We have now solved the Pangolin Riddle! This is BIG so sit tight! 1. WIV did have Pangolins in 2019 (we now have proof) 2. WIV isolated Pangolin/HKU4/MERS like Covs with Furin Sites in 2019, just before the pandemic!

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

Pangolin Riddle! MpCoV-GX Isolated in 2017 said YiGang Tong BUT ZLS & Yi GangTong in 2023 said: "MpCoV-GX was isolated in 2019" SARS-CoV-2-Related Virus from Malayan Pangolin https://pmc.ncbi.nlm.nih.gov/articles/PMC9972989/#s2 Accession data says submission date is 8 FEB 2020 https://www.ncbi.nlm.nih.gov/nuccore/MT040334.1

A SARS-CoV-2-Related Virus from Malayan Pangolin Causes Lung Infection without Severe Disease in Human ACE2-Transgenic Mice Coronavirus disease 2019 (COVID-19), which is caused by the novel coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is the most severe emerging infectious disease in the current century. The discovery of SARS-CoV-2-related ... pmc.ncbi.nlm.nih.gov
Pangolin coronavirus isolate PCoV_GX-P1E, complete genome - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

3. Important Note If you have not done so already, please read the linked thread above before continuing, it will make much more sense if you do so.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

4. GX Pangolins at WIV in 2019! From the Horse's Mouth in 2024! "In 2019 & 2020, 86 anal swab samples & 80 serum samples were collected from Guangxi pangolins" "Pangolin samples were transported & stored at Wuhan Institute of Virology, CAS" https://www.cell.com/cell/fulltext/S0092-8674(23)00049-1

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

5. Pangolin MERS Cov Clones Cell Paper received June 2022 WIV does not say when viral isolates from pangolin samples were obtained & experimented with BUT research of @stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin shows that a MERS/HKU4 clone was used before Jan 2020!

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin 6. NCBI BioProject PRJNA602160 We identified SRA datasets with HKU4-related & MERS-related CoV sequences Datasets generated by HZAU Registered: 2020-01-19 Published: 2020-02-09 Discovery of a Novel MERS DNA Clone Contaminating Sequencing Datasets https://www.fortunejournals.com/articles/discovery-of-a-novel-merbecovirus-dna-clone-contaminating-agricultural-rice-sequencing-datasets-from-wuhan-china.html

Discovery of a Novel Merbecovirus DNA Clone Contaminating Agricultural Rice Sequencing Datasets from Wuhan, China Discovery of a Novel Merbecovirus DNA Clone Contaminating Agricultural Rice Sequencing Datasets from Wuhan, China. PubMed, SCI, Scopus, ESCI, PMC indexed fortunejournals.com

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin 7. Above claims recently confirmed by WIV patent! Also BAC finding confirmed h/t @Rcoleptic and @Engineer2The MERS cluster pangolin coronavirus MjHKU4r CoV-1replicon plasmid, cell line and application Published in Sept 2023 https://patents.google.com/patent/CN117305361A/en?oq=CN117305361

@Engineer2The - TheEngineer2 🇨🇦

1/ What do we have here? "MERS cluster pangolin coronavirus MjHKU4r CoV-1 replicon plasmid, cell line and application" - Shi Zhengli From CN117305361

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin @Rcoleptic @Engineer2The 8. Image of Replicon Plasmid from the Patent Note Bsmb 1 restriction stes used https://patentimages.storage.googleapis.com/33/e4/c9/2fc8abe09c9555/CN117305361A.pdf

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin @Rcoleptic @Engineer2The 9. The Ubiquitous Yi-Gang Tong Proudly discusses the mechanism by which a bat MERS-like coronavirus infects host cells 1. MjHKU4r-CoV virus discovered in GX pangolins 2. Similarities between a furin cleavage site (RQQR) in SD1 of S protein & MERS https://en-life.buct.edu.cn/2024/0520/c2446a192266/page.htm

Beijing University of Chemical Technology Tong Yigang Research Group reveals in "Cell" a molecular mechanism by which a bat MERS-like coronavirus infects host cells. On February 16, 2023, the top international academic journal Cell published online a research paper titled A bat MERS-like coronavirus circulates in pangolins and utilizes human DPP4 and host proteases for cell entry, reporting on the molecular mechanism of a new MERS-related coronavirus (Manis javanica HKU4-related coronavirus, MjHKU4r-CoV) infecting human cells. Li Jing, a doctoral student at Beijing University of Chemical Technology, was listed as a co-first author of the article, and Profess en-life.buct.edu.cn

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin @Rcoleptic @Engineer2The 10. Continued 3. MjHKU4r-CoV-1 infection was significantly affected by furin protease inhibitors. 4. Mutation experiments at the furin cleavage site in the S protein of MjHKU4r-CoV-1 demonstrated the crucial role of host protease cleavage during infection.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin @Rcoleptic @Engineer2The 11. Continued (2) 5. They used various cell lines & organoids to observe infectivity of MjHKU4r-CoV-1 6. MjHKU4r-CoV-1 can infect various human cell lines & replicate in human respiratory & intestinal organoids 7. Also in vivo infection experiments in hDPP4 transgenic mice

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin @Rcoleptic @Engineer2The 12. Forensic Genomics 8. The ratio of viral to animal reads can show if an animal is a likely host or not. 9. Presence of Pangolin isolate GX/HKU4-GX/2020 in the S. scrofa NGS dataset & similarity to pangolin CoV MjHKU4r-CoV-1 points to a lab construct! https://www.news-medical.net/news/20230622/Pangolin-study-uncovers-novel-strains-of-coronaviruses-and-raises-concerns-about-origin.aspx

Pangolin study uncovers novel strains of coronaviruses and raises concerns about origin Researchers identify novel HKU4-related coronavirus in a pangolin single-cell sequencing dataset. news-medical.net

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin @Rcoleptic @Engineer2The 13. How does all this relate to SARS-COV-2? It is not yet known how SARS-COV-2 acquired its FCS. This thread shows that SC-2 may have acquired its FCS via cell culture contamination involving GX Pangolin MERS Cov isolates (MjHKU4r-CoV-1) stored & experimented on at WIV in 2019.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin @Rcoleptic @Engineer2The 14. Why is MjHKU4r-CoV-1 special? 1. FCS are more common in MERS Covs but in the HKU4 group, no such FCS exists. 2. Substitution of KQQR motif at the S1/S2 junction in the bat HKU4 to the RQQR found in MjHKU4r-CoV-1 results in the generation of a minimal consensus FCS (RXXR)!

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin @Rcoleptic @Engineer2The 15. Why is MjHKU4r-CoV-1 special? (2) 3. MjHKU4r-CoV-1 Spike is processed by furin 4. FCS enhances viral entry into human cells by 2–3 orders of magnitude compared to bat HKU4 5. Thus, MjHKU4r-CoV-1 Spike has acquired a functioning FCS that conferentry into human cells

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin @Rcoleptic @Engineer2The 16. References for the above 3 statements By guess who? None other than our our old friend Stuart JD Neil 😂 who tries to interpret the findings in a "Zoonotic" light See image Pangolin merbecovirus gets down to (poly)basics https://wfmclib.yuntsg.com/official/fwinfo.html?id=15586&type=1&typeId=2 https://bookcafe.yuntsg.com/ueditor/jsp/upload/file/20230306/1678090286940021156.pdf

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin @Rcoleptic @Engineer2The 17. A Logical Chain & Evidence have now been found What remains is the question of the exact pathway and experiments that that led to the acquisition of an FCS in Sars-COV-2 in the laboratories of the WIV. No wonder they refused to share their lab notebooks!

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin @Rcoleptic @Engineer2The 18. A possible pathway (now more likely) For that, see Bernd Kaina's Paper: "On the Origin of SARS-CoV-2: Did Cell Culture Experiments Lead to Increased Virulence of the Progenitor Virus for Humans?" https://iv.iiarjournals.org/content/35/3/1313

On the Origin of SARS-CoV-2: Did Cell Culture Experiments Lead to Increased Virulence of the Progenitor Virus for Humans? We are currently in a rapidly expanding pandemic of the SARS-CoV-2 virus, which originated in the city of Wuhan in central China. The COVID-19 is now spread worldwide and has tremendous socio-economic consequences. The origin of the virus can be reconstructed through epidemiological studies and, even more so, from genome comparisons. How the evolution of the virus and the transition to humans might have happened is the subject of much speculation. It is considered certain that the virus is of animal origin and very likely passed from bats to humans in a zoonotic event. An intermediate host was postulated, but many SARS-like bat viruses have the ability to infect human cells directly, which has been shown experimentally by scientists in the Wuhan Institute of Virology using collected specimens containing virus material from horseshoe bats. The propagation of SARS-like bat viruses in cell culture allowed experiments aimed at increasing the infectivity of the virus and adaptation to human cells. This article summarizes the unique properties of SARS-CoV-2 and focusses on a specific sequence encoding the spike protein. Possible scenarios of virus evolution are discussed, with particular emphasis on the hypothesis that the virus could have emerged unintentionally through routine culture or gain-of-function experiments in a laboratory, where it was optimally adapted to human cells and caused cryptic infections among workers who finally spread the virus causing the pandemic. iv.iiarjournals.org

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin @Rcoleptic @Engineer2The 19. Bernd Kaina (1) 1. There is a remarkable identity on amino acid & nucleotide level in & around the FCS between SARS-CoV-2 & a MERS-CoV 2. This supports the hypothesis that the PCS/furin cleavage site was gained by a recombination event(s) involving these virus sequences. https://t.co/KRr9rn9SWK

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin @Rcoleptic @Engineer2The 20. Bernd Kaina (2) 3. When cells in culture were coinfected with the predecessor of SARS-CoV-2 and another virus strain that contains the PCS/furin cleavage site, the sequence was transferred to the predecessor virus as a result of a recombination event.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin @Rcoleptic @Engineer2The 21. Bernd Kaina (3) 4. Interestingly, MERS-CoV contains a furin-specific cleavage site, which is very similar to the one found in SARS-CoV-2. As outlined above, there are 6 identical amino acids between position 678 and 687 in SARS-CoV-2 and MERS-CoV.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin @Rcoleptic @Engineer2The 22. Bernd Kaina (4) 5. Gain of function through the PCS could have happened unintentionally, e.g. after introducing different viruses into a cell to see whether they complement each other functionally (“in trans”), or purposefully in the context of gain-of-function experiments.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin @Rcoleptic @Engineer2The 23. Bernd Kaina (5) 6. Propagation of the progenitor of SARS-CoV-2 (e.g. RaTG13) together with a MERS-CoV is likely to lead to the selection of a virus that gains functions of both viruses, thus increasing its infection rate in vitro.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin @Rcoleptic @Engineer2The 24. Bernd Kaina (6) 7. The cells used were equipped with the ACE2 receptor. If these cells also harbored the neuropilin-1 membran protein, selection for a virus showing an even higher virus titer upon propagation in vitro is conceivable.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin @Rcoleptic @Engineer2The 25. Bernd Kaina (7) 8. It should be noted that cell lines harboring ACE2 together with NRP1 and other desired properties can be generated by routine techniques (transient or stable transfection, lentivirus transduction) and have actually been used for experimental purposes.

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin @Rcoleptic @Engineer2The 26. Summary (1) The lab hypothesis does not posit that SARS-CoV-2 was genetically engineered on purpose. The hypothesis tates that SARS-CoV-2 is an unintended byproduct of gain-of-function & cotransfection experiments using human cell lines in vitro. https://t.co/80kB3HYevX

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin @Rcoleptic @Engineer2The 27. Summary (2) There are many conceivable scenarios how transmission could occur in the lab, e.g. through aerosols during the work or handling of waste. Such lab events could have occurred repeatedly long before December 2019. They remained undetected with cryptic spread. https://t.co/lbkPDHq4fS

@BillyBostickson - Billy Bostickson 🏴👁&👁 🆓

@stevenemassey @quay_dr @Daoyu15 @humblesci @ydeigin @Rcoleptic @Engineer2The Unroll Pangolin Riddle Solved @threadreaderapp

Saved - February 1, 2025 at 10:20 AM
reSee.it AI Summary
Holmes analyzed the submission of 60 viruses in a 2018 preprint, revealing only 163 of a potential 180 sequences were included. Current data shows 154 sequences in GenBank, with interruptions in submissions dating from October 2019. This raises questions about 9 missing ORF8 genes and several S genes. The conversation highlights ongoing efforts to recover this missing data, suggesting that the disclosures may be incomplete and emphasizing the need for thorough verification in scientific research.

@tommy_cleary - Tommy Cleary

Holmes attempted <> methods, ...with his Twitter thread on March 6th 2023, ...as evidence that the 60 viruses submitted as part of a preprint, together with Prof Jie Cui and ZLShi of WIV, were complete but only 163 of a potential 180 sequences were part of this 12-JUL-2018 PrePrint? Only 154 of those are in the current GI series available to be recovered... as far as I know...with this current GI series of 154 submissions is interrupted by submissions dated 25-OCT-2019 and the ACCESSION series continuing from the last, with <> to <> which is unrelated but dated Jul 13, 2019 08:18 PM. https://ncbi.nlm.nih.gov/nuccore/MH615993.1?report=girevhist This suggests that the original GenBank submission, perhaps actually of 180 sequences, was cropped to 163 and given new ACCESSION numbers one year after it was submitted...with the cropped series of 163 placed in their current GI position on 25-OCT-2019...but what of the missing 9 ORF8 from this GI series? Methods: basic GI series analysis this post GI is 1769824416 https://ncbi.nlm.nih.gov/nuccore/1769824416 ...next will be 1769824414 So <> is the next missing OFR8 gene for <> GenBank submission from @syd_health 's & @Sydney_Uni 's Prof Edward Holmes @EdwardCHolmes to GenBank of @NLM_NIH soon to be headed by @DrJBhattacharya So now I am trying to help Holmes & @syd_health recover the missing data NOW tally is at eleven missing ORF8 and three missing S genes... where for <> RdRp is the there: https://ncbi.nlm.nih.gov/nuccore/MH615889.1?report=genbank and S gene is there but supressed: https://www.ncbi.nlm.nih.gov/nuccore/1769824528 but no ORF8 gene in this GI series Why? Missing ORF8 tally so far: 1) Rs151334_Guizhou 2) Rf131405_Shanxi 3) Rs140400_Guangdong 4) Rs141456_Guangxi 5) Rspp7924_Yunnan 6) Rspp7921_Yunnan 7) Ra7909_Yunnan 8) Rspp7907_Yunnan 9) Rspp7905_Yunnan 10) Rspp7896_Yunnan 11) Rs6303_Yunnan of a total of 15 missing... Question: Was <> in the 60-54= 6 ORF8 that <> decided to leave out of this 2018 PrePrint... ? https://web.archive.org/web/20220809085043/https://www.ncbi.nlm.nih.gov/nuccore/?term=Spread+and+Geographic+Structure+of+SARS-related+Coronaviruses+in+++++++++++++Bats+and+the+Origin+of+Human+SARS+Coronavirus ...or perhaps the 54-45= 9 ORF8 that are simply missing from the GI series suppressed in GenBank & interrupted by the date 25-Oct-2019? With S genes missing too; of the 60 RdRp sampled only 49 S genes are here in this GI series...Why? 1) Rspp7921_Yunnan 2) Rspp7907_Yunnan 3) Rspp7896_Yunnan of 11 S genes left out of this study. Why? <> S gene is there but seems quite different to others. Why? All this so far indicates that the 2023 @COVIDSelect disclosures of Holmes are potentially incomplete...but the count continues... next search is GI 1769824414! Taxonomy browser (Bat SARS-like coronavirus) https://archive.md/qgC9W#selection-2037.0-2081.1

Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs6303_Yunnan RNA-dependent RNA polym - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs6303_Yunnan RNA-dependent RNA polym - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs6303_Yunnan spike protein (S) gene, - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Spread and Geographic Structure of SARS-related Coronaviruses in Bats - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube web.archive.org

@tommy_cleary - Tommy Cleary

One more before I put the roast on for Australia Day dinner... @GrahamPerrettMP is my local Federal MP and he has helped in the past, but last time I wrote to him he replied that I should check the Queensland State Library for more details...perhaps I should check back with him again too. These issues of how to handle the dangerous side of science have been a problem since at least Iraq's @UN biological weapons inspections...with discussions of Mustard brought to the table by @R_H_Ebright thank you, @INTERPOL_CBRNE questions are important. H/t @CharlesRixey @Ayjchan @Globalbiosec Holmes attempted <> methods, ...with his Twitter thread on March 6th 2023, ...as evidence that the 60 viruses submitted as part of a preprint, together with Prof Jie Cui and ZLShi of WIV, were complete... but only 163 of a potential 180 sequences were part of this 12-JUL-2018 PrePrint? https://web.archive.org/web/20220809085043/https://www.ncbi.nlm.nih.gov/nuccore/?term=Spread+and+Geographic+Structure+of+SARS-related+Coronaviruses+in+++++++++++++Bats+and+the+Origin+of+Human+SARS+Coronavirus Only 154 of those are in the current GI series available to be recovered... as far as I know...with this current GI series of 154 submissions is interrupted by submissions dated 25-OCT-2019 and the ACCESSION series continuing from the last, with <> to <> which is unrelated but dated Jul 13, 2019 08:18 PM. https://ncbi.nlm.nih.gov/nuccore/MH615993.1?report=girevhist This suggests that the original GenBank submission, perhaps actually of 180 sequences, was cropped to 163 and given new ACCESSION numbers one year after it was submitted...with the cropped series of 163 placed in their current GI position on 25-OCT-2019...but what of the missing 9 ORF8 from this GI series? KISS Methods: basic GI series analysis this post GI is 1769824414 anyone can do this... https://ncbi.nlm.nih.gov/nuccore/1769824414 but with <> nothing is missing, all three sets are there in GenBank ORF8, S and RdRp...and apparently has identical RBD to As6526? <> https://www.ncbi.nlm.nih.gov/nuccore/KY417142 So...next will be 1769824412 <> also all three accounted for too and even features in the <>... https://www.ncbi.nlm.nih.gov/nuccore/MH615887.1?report=girevhist So, next is 1769824410...Bingo! <> ORF8 missing! So <> is the next missing OFR8 gene for <> GenBank submission from @syd_health 's & @Sydney_Uni 's Prof Edward Holmes @EdwardCHolmes to GenBank of @NLM_NIH soon to be headed by @DrJBhattacharya @secrubio & @RobertKennedyJr in the mix too. So now I am trying to help Holmes & @syd_health recover the missing GenBank data...for everyone that hungers for a slice of truth tune in... NOW tally is at twelve missing ORF8 and three missing S genes... where for <> RdRp is the there: https://www.ncbi.nlm.nih.gov/nuccore/MH615886.1?report=genbank and S gene is there but suppressed: https://www.ncbi.nlm.nih.gov/nuccore/1769824532 but no ORF8 gene in this GI series Why? Missing ORF8 tally so far: 1) Rs151334_Guizhou 2) Rf131405_Shanxi 3) Rs140400_Guangdong 4) Rs141456_Guangxi 5) Rspp7924_Yunnan 6) Rspp7921_Yunnan 7) Ra7909_Yunnan 8) Rspp7907_Yunnan 9) Rspp7905_Yunnan 10) Rspp7896_Yunnan 11) Rs6303_Yunnan 12) Rs6266_Yunnan of a total of 15 missing... Question: Was <> in the 60-54= 6 ORF8 that <> decided to leave out of this 2018 PrePrint... ...or perhaps the 54-45= 9 ORF8 that are simply missing from the GI series suppressed in GenBank & interrupted by the date 25-Oct-2019? With S genes missing too; of the 60 RdRp sampled only 49 S genes are here in this GI series...Why? 1) Rspp7921_Yunnan 2) Rspp7907_Yunnan 3) Rspp7896_Yunnan of 11 S genes left out of this study. Why? <> S gene is there but seems quite different to others. Why? All this so far indicates that the 2023 @COVIDSelect disclosures of Holmes are potentially incomplete...but the count continues... next search is GI 1769824408! Taxonomy browser (Bat SARS-like coronavirus) https://archive.md/qgC9W#selectio ...speaking of things that are difficult to understand... Any idea of how it is that @BrookeNGenovese reasonable Sep 2020 appeal to have the suspended@Twitteraccounts for:@PREDICTProject@OneHealthLabs@GlobalVirome@HALIUCDavis has gone? Perhaps some are up & running, perhaps others are still suppressed? <<@TwitterSupport also, the lab’s account @OneHealthLab &the Global Virome Project @GlobalVirome are similarly suspended..since June...despite repeated attempts to resolve. 🤨 @Twitter oh and the @HALIUCDavis account, too. Anyone noticing a theme here...?>> How is @RogerMarshallMD & @COVIDSelect going to discuss this issue with the public if the terms are suppressed? Things to chew over dinner roast?

Spread and Geographic Structure of SARS-related Coronaviruses in Bats - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube web.archive.org
Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs160665_Yunnan RNA-dependent RNA pol - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Bat SARS-like coronavirus isolate As6526, complete genome - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs6266_Yunnan RNA-dependent RNA polym - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs6266_Yunnan spike protein (S) gene, - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov

@tommy_cleary - Tommy Cleary

@Studio28nyc @McWLuke @PeterDaszak @WHO @SciDiplomacyUSA @BangXiao_ @POTUS After Australia Day roast lunch (which was fantastic) I sent another email this time to Zhengli Shi & @BangXiao_ Then me & the fam went to local barefoot lawn bowls.

@R_H_Ebright - Richard H. Ebright

Only mustard at US base in Iraq was on condiments tray in mess hall #Disinformation @R_H_Ebright

@BrookeNGenovese - Brooke Genovese

Reviving this because @PREDICTProject is inexplicably suspended again SMH @TwitterSupport

@tommy_cleary - Tommy Cleary

@BrookeNGenovese @twitter @PREDICTproject Not gone, just suspended You can find @PREDICTproject here

@tommy_cleary - Tommy Cleary

Seeking No14 ORF8 omission...with some healthy distraction from @breakfast_dogs @harishseshadri2 @gdemaneuf about the truisms of love...and knowing at all. @Rebecca21951651 @emilyakopp @a_kruschke @Ayjchan @VBruttel @BillyBostickson Back to the data set. Holmes attempted <> methods,@MarionKoopmans ...with his Twitter thread on March 6th 2023, ...as evidence that the 60 viruses submitted as part of a preprint, together with Prof Jie Cui and ZLShi of WIV, were complete... https://journals.asm.org/doi/10.1128/jvi.01240-24 ...but only 163 of a potential 180 sequences were part of this 12-JUL-2018 PrePrint? https://web.archive.org/web/20220809085043/https://www.ncbi.nlm.nih.gov/nuccore/?term=Spread+and+Geographic+Structure+of+SARS-related+Coronaviruses+in+++++++++++++Bats+and+the+Origin+of+Human+SARS+Coronavirus Only 154 of those are in the current GI series available to be recovered... as far as I know... Note important under examined bioinformatics data: ...with this current GI series of 154 submissions is interrupted by submissions dated 25-OCT-2019 and the ACCESSION series continuing from the last, with <> to <> which is unrelated but dated Jul 13, 2019 08:18 PM. https://ncbi.nlm.nih.gov/nuccore/MH615993.1?report=girevhist This suggests that the original GenBank submission, perhaps actually of 180 sequences, was cropped to 163 and given new ACCESSION numbers one year after it was submitted...with the cropped series of 163 placed in their current GI position on 25-OCT-2019...but what of the missing 9 ORF8 from this GI series? GI count is hypothesized as a way of delineating this Undone Science data set. KISS Methods: basic GI series analysis this Xpost GI is 1769824408 anyone can do this... https://ncbi.nlm.nih.gov/nuccore/1769824408 but with <> nothing is missing, all three sets are there in GenBank ORF8, S and RdRp... So...next will be 1769824406 <> also all three accounted for too...but getting close to the typology of another hidden data set from Beijing Institute of Microbiology and Epidemiology? <> isolate missing isolation source sputum collection date 2019 geographic location China: HeNan>> https://www.ncbi.nlm.nih.gov/biosample/28539355 So, next is 1769824404 <> all there...but this is where the RdRp set ends and so GI for 1769824404 is < Next is 1769824402 <> all there... ///////// Hmm interesting data links here: NOTE homework <> @quay_dr @MartinaSisters <> https://pubmed.ncbi.nlm.nih.gov/31022925/ /////// Next is 1769824400 <> all three there Next is 1769824398 <> all three there Note: duplication issues here with S gene? Next is 1769824396 <> Tombola! Ambo...you see ORF8 and RdRp are here but for <> the S gene is missing. Why? So <> is the next missing data point for <> GenBank submission from @syd_health 's & @Sydney_Uni 's Prof Edward Holmes @EdwardCHolmes to GenBank of @NLM_NIH soon to be headed by@DrJBhattacharyateamed with@secrubio& @RobertKennedyJr by @POTUS So now I am trying to help Holmes & @syd_health recover the missing GenBank data...for everyone that hungers for a slice of truth tune in... NOW tally is still twelve missing ORF8 and now four missing S genes... where for <> RdRp is the there: https://www.ncbi.nlm.nih.gov/nuccore/1769824500 ORF8 gene is there but suppressed: https://www.ncbi.nlm.nih.gov/nuccore/1769824396 but no S gene in this GI series Why? Missing ORF8 tally so far: 1) Rs151334_Guizhou 2) Rf131405_Shanxi 3) Rs140400_Guangdong 4) Rs141456_Guangxi 5) Rspp7924_Yunnan 6) Rspp7921_Yunnan 7) Ra7909_Yunnan 8) Rspp7907_Yunnan 9) Rspp7905_Yunnan 10) Rspp7896_Yunnan 11) Rs6303_Yunnan 12) Rs6266_Yunnan of a total of 15 missing... Question: Was <> in the 60-54= 6 ORF8 that <> decided to leave out of this 2018 PrePrint... ...or perhaps the 54-45= 9 ORF8 that are simply missing from the GI series suppressed in GenBank & interrupted by the date 25-Oct-2019? With S genes missing too; of the 60 RdRp sampled only 49 S genes are here in this GI series...Why? 1) Rspp7921_Yunnan 2) Rspp7907_Yunnan 3) Rspp7896_Yunnan 4) Rs9214_Hubei of 11 S genes left out of this study. Why? <> S gene is missing Why? All this so far indicates that the 2023 @COVIDSelect disclosures of Holmes are potentially incomplete...but the count continues... next search is GI 1769824394! ////// Suppression and Dissent in Science is such an interest topic https://documents.uow.edu.au/~bmartin/pubs/99rsppp.html ...my MA thesis Professor is very good in this area Brian Martin and also has good advice about academic reading and writing...a little every day. COVID Origin Case study is full of under examined data. EG Such an interesting set of STS & Philosophy of Science discourse data: Q/ What exactly here is so controversial? @PREDICTProjectarchive is good & interesting: @OneHealthLabsarchive @waybackmachine is too late: @HALIUCDavis archive doesn't look that useful: @GlobalVirome archive: One Health Institute (OHI) @OneHealthUCD any ideas? < ///// Oh well. next missing data point starts with the ORF8 GI 1769824394 searching for more missing S genes, I think? A little each day.

Spread and Geographic Structure of SARS-related Coronaviruses in Bats - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube web.archive.org
Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs6255_Yunnan RNA-dependent RNA polym - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
not collected - BioSample - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Characterization of a New Member of Alphacoronavirus with Unique Genomic Features in Rhinolophus Bats - PubMed Bats have been identified as a natural reservoir of a variety of coronaviruses (CoVs). Several of them have caused diseases in humans and domestic animals by interspecies transmission. Considering the diversity of bat coronaviruses, bat species and populations, we expect to discover more bat CoVs th … pubmed.ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs9214_Hubei RNA-dependent RNA polyme - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs9214_Hubei ORF8 gene, complete cds - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov

@tommy_cleary - Tommy Cleary

@BillyBostickson @Rebecca21951651 @gdemaneuf @CIA The love theory? Message in a bottle? They had a child called NoWay? These are all great ideas from a Cognitive Science perspective… As a philosopher of Science… life and knowing is never so simple as it seems… People are people and some are talking very…

@VBruttel - Dr. rer. nat. Valentin Bruttel

Why SARS-CoV-2 was a lab manipulated virus in 10 key points https://vbruttel.substack.com/p/why-sars-cov-2-was-a-lab-manipulated The IMO most compelling molecular and circumstantial evidence regarding the origin of COVID-19. ➡️ Please share, retweet, and raise awareness to help prevent similar events from occurring again.

Why SARS-CoV-2 was a Lab-Manipulated Virus, in 10 Key Points SARS-CoV-2 exhibits specific alterations that align so precisely with a research proposal that, combined with circumstantial evidence, they prove a laboratory origin beyond reasonable doubt. vbruttel.substack.com

@MarionKoopmans - Marion Koopmans, publications: https://pure.eur.nl

@VBruttel the real route should be: submit for peer review in a credible journal

@tommy_cleary - Tommy Cleary

@R_H_Ebright @ScienceMagazine Further...as much as Holmes has stated that data from Jie Cui was not linked to WIV 162 of 163 submissions to GenBank remain suppressed or missing...with other serious data integrity issues and cyber biosecurity issues needing to be addressed... Disqualifying conflict of…

@tommy_cleary - Tommy Cleary

@_everythingism @AceBearstrom @hiltzikm STS studies regularly acknowledge and explore institutional limits to knowledge away from the political narratives you outline here. <<Undone Science Social Movements, Mobilized Publics, and Industrial Transitions By David J. Hess>> https://mitpress.mit.edu/9780262529495/undone-science/ Since this research…

Undone Science A theoretical integration of science and technology studies and social movement studies that finds both common ground and “undone” research.As the fields... mitpress.mit.edu

@StoreEducation - EducationStore

Writing how to be more productive without procrastinating or bingeing UOW: University of Wollongong, Australia Speaker: Emeritus Prof. Brian Martin and members of PhD Candidates Date: 09/02/2020 Time: 11:30 AM Canberra, Melbourne, Sydney Register NOW: https://zoom.us/webinar/register/WN_aA-y9bEaQKKO4fTdu_oVxw

Video Conferencing, Web Conferencing, Webinars, Screen Sharing Zoom is the leader in modern enterprise video communications, with an easy, reliable cloud platform for video and audio conferencing, chat, and webinars across mobile, desktop, and room systems. Zoom Rooms is the original software-based conference room solution used around the world in board, conference, huddle, and training rooms, as well as executive offices and classrooms. Founded in 2011, Zoom helps businesses and organizations bring their teams together in a frictionless environment to get more done. Zoom is a publicly traded company headquartered in San Jose, CA. zoom.us

@tommy_cleary - Tommy Cleary

But there is poetry in these lethal paragraphs of RNA H/t @quay_dr @MartinaSisters Where have the poets of this world gone? Why have rhymes bent to reason and quills been put aside to crumble ? What feeble mind thinks yet does not imagine possibilities of other minds too? Minds seek minds within what we all wonder

@tommy_cleary - Tommy Cleary

The question of //Pathos// has disturbed the search for the next missing part of this data set. Never a better reason to interrupt seeking is finding a question linked to the heart. Knowing love is a perennial concern. To leave souls behind has a sharp gravitas. Back to the data. In 2023 Holmes attempted <> methods,with his Twitter thread on March 6th 2023, ...as evidence that the 60 viruses submitted as part of a preprint, together with Prof Jie Cui and ZLShi of WIV, were complete... https://journals.asm.org/doi/10.1128/jvi.01240-24 ...but only 163 of a potential 180 sequences were part of this 12-JUL-2018 PrePrint? https://web.archive.org/web/20220809085043/https://www.ncbi.nlm.nih.gov/nuccore/?term=Spread+and+Geographic+Structure+of+SARS-related+Coronaviruses+in+++++++++++++Bats+and+the+Origin+of+Human+SARS+Coronavirus Only 154 of those are in the current GI series available to be recovered... as far as I know... this thread tests these assumptions & knowledge claims. Note important under examined bioinformatics data: ...with this current GI series of 154 submissions is interrupted by submissions dated 25-OCT-2019 and the ACCESSION series continuing from the last, with <> to <> which is unrelated but dated Jul 13, 2019 08:18 PM. https://ncbi.nlm.nih.gov/nuccore/MH615993.1?report=girevhist This suggests that the original GenBank submission, perhaps actually of 180 sequences, was cropped to 163 and given new ACCESSION numbers one year after it was submitted...with the cropped series of 163 placed in their current GI position on 25-OCT-2019...but what of the missing 9 ORF8 from this GI series? GI count is hypothesized as a way of delineating this Undone Science data set. KISS Methods: basic GI series analysis this Xpost GI is 1769824394 <> anyone can do this... https://ncbi.nlm.nih.gov/nuccore/1769824394 Bingo GI 1769824394 <> ! Again ORF8 and RdRp are here but for <> the S gene is missing. Why? So <> is the next missing data point for <> GenBank submission from @syd_health 's & @Sydney_Uni's Prof Edward Holmes @EdwardCHolmes to GenBank of@NLM_NIH NOW tally is still twelve missing ORF8 and now five missing S genes... where for <> RdRp is the there: https://www.ncbi.nlm.nih.gov/nuccore/1769824498 ORF8 gene is there but suppressed: https://www.ncbi.nlm.nih.gov/nuccore/1769824394 but no S gene in this GI series Why? Missing ORF8 tally so far: 1) Rs151334_Guizhou 2) Rf131405_Shanxi 3) Rs140400_Guangdong 4) Rs141456_Guangxi 5) Rspp7924_Yunnan 6) Rspp7921_Yunnan 7) Ra7909_Yunnan 8) Rspp7907_Yunnan 9) Rspp7905_Yunnan 10) Rspp7896_Yunnan 11) Rs6303_Yunnan 12) Rs6266_Yunnan of a total of 15 missing... Question: Was <> in the 60-54= 6 ORF8 that <> decided to leave out of this 2018 PrePrint... ...or perhaps the 54-45= 9 ORF8 that are simply missing from the GI series suppressed in GenBank & interrupted by the date 25-Oct-2019? With S genes missing too; of the 60 RdRp sampled only 49 S genes are here in this GI series... Why? 1) Rspp7921_Yunnan 2) Rspp7907_Yunnan 3) Rspp7896_Yunnan 4) Rs9214_Hubei 5) Rs9201_Hubei of 11 S genes left out of this study. Why? <> S gene is missing Why? All this so far indicates that the 2023@COVIDSelectdisclosures of Holmes are potentially incomplete...but the count continues... next search is GI 1769824392!

Spread and Geographic Structure of SARS-related Coronaviruses in Bats - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube web.archive.org
Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs9201_Hubei ORF8 gene, complete cds - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs9201_Hubei RNA-dependent RNA polyme - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs9201_Hubei ORF8 gene, complete cds - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov

@tommy_cleary - Tommy Cleary

@gdemaneuf There is a theory that Drosten changed his mind…or was more free to speak his mind…after Shi made it out of China recently…nice idea. People. People do what people do…they fall in love and do stupid and sometimes inspirational things.

@tommy_cleary - Tommy Cleary

@R_H_Ebright @ScienceMagazine Further...as much as Holmes has stated that data from Jie Cui was not linked to WIV 162 of 163 submissions to GenBank remain suppressed or missing...with other serious data integrity issues and cyber biosecurity issues needing to be addressed... Disqualifying conflict of…

@tommy_cleary - Tommy Cleary

@_everythingism @AceBearstrom @hiltzikm STS studies regularly acknowledge and explore institutional limits to knowledge away from the political narratives you outline here. <<Undone Science Social Movements, Mobilized Publics, and Industrial Transitions By David J. Hess>> https://mitpress.mit.edu/9780262529495/undone-science/ Since this research…

Undone Science A theoretical integration of science and technology studies and social movement studies that finds both common ground and “undone” research.As the fields... mitpress.mit.edu

@tommy_cleary - Tommy Cleary

In 2023 Holmes attempted <> methods appropriate of bioweapons investigations, see @CharlesRixey ...with Eddie's Twitter thread on March 6th 2023, here: ...as evidence that the 60 viruses submitted as part of a preprint, together with Prof Jie Cui and ZLShi of WIV, were complete... https://journals.asm.org/doi/10.1128/jvi.01240-24 ...but only 163 of a potential 180 sequences were part of this 12-JUL-2018 PrePrint? https://web.archive.org/web/20220809085043/https://www.ncbi.nlm.nih.gov/nuccore/?term=Spread+and+Geographic+Structure+of+SARS-related+Coronaviruses+in+++++++++++++Bats+and+the+Origin+of+Human+SARS+Coronavirus But only 154 of these are in the current GI series available to be recovered... as far as I know... this thread tests these assumptions & knowledge claims. //Note important under examined bioinformatics data: ...with this current GI series of 154 submissions is interrupted by submissions dated 25-OCT-2019 and the ACCESSION series continuing from the last, with <> to <> which is unrelated but dated Jul 13, 2019 08:18 PM. https://ncbi.nlm.nih.gov/nuccore/MH615993.1?report=girevhist This suggests that the original GenBank submission, perhaps actually of 180 sequences, was cropped to 163 and given new ACCESSION numbers one year after it was submitted...with the cropped series of 163 placed in their current GI position on 25-OCT-2019...but what of the missing 9 ORF8 from this GI series? GI count is hypothesized as a way of delineating this Undone Science data set. /// KISS Methods: basic GI series analysis this Xpost GI is 1769824392 <> anyone can do this... even @stgoldst or perhaps @tgof137 @VICENews @ChrisCillizza @zerohedge even? https://ncbi.nlm.nih.gov/nuccore/1769824392 GI 1769824392 <> all good GI 1769824390 <> all good GI 1769824390 <> BINGO! Again ORF8 and RdRp are here but for <> the S gene is missing. Why? So <> is the next missing data point for <> GenBank submission from @syd_health 's & @Sydney_Uni 's Prof Edward Holmes @EdwardCHolmes to GenBank of @NLM_NIH NOW tally is still twelve missing ORF8... and now six missing S genes... where for <> RdRp is the there: https://www.ncbi.nlm.nih.gov/nuccore/1769824496 ORF8 gene is there but both suppressed: https://www.ncbi.nlm.nih.gov/nuccore/1769824388 but no S gene in this GI series Why? Missing ORF8 tally so far: 1) Rs151334_Guizhou 2) Rf131405_Shanxi 3) Rs140400_Guangdong 4) Rs141456_Guangxi 5) Rspp7924_Yunnan 6) Rspp7921_Yunnan 7) Ra7909_Yunnan 8) Rspp7907_Yunnan 9) Rspp7905_Yunnan 10) Rspp7896_Yunnan 11) Rs6303_Yunnan 12) Rs6266_Yunnan identified of a total of 15 missing... Question: Was <> in the 60-54= 6 ORF8 that <> decided to leave out of this 2018 PrePrint... ...or perhaps the 54-45= 9 ORF8 that are simply missing from the GI series suppressed in GenBank & interrupted by the date 25-Oct-2019? With S genes missing too; of the 60 RdRp sampled only 49 S genes are here in this GI series... Why? 1) Rspp7921_Yunnan 2) Rspp7907_Yunnan 3) Rspp7896_Yunnan 4) Rs9214_Hubei 5) Rs9201_Hubei 6) Rs151199_Hunan identified of 11 S genes left out of this study. Why? <> S gene is missing Why? All this so far indicates that the 2023 @COVIDSelect disclosures of Holmes are potentially incomplete...but the count continues... next search is GI 1769824386!

Spread and Geographic Structure of SARS-related Coronaviruses in Bats - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube web.archive.org
Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs151239_Hunan ORF8 gene, complete cd - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs151199_Hunan RNA-dependent RNA poly - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs151199_Hunan ORF8 gene, complete cd - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov

@tommy_cleary - Tommy Cleary

@R_H_Ebright @ScienceMagazine Further...as much as Holmes has stated that data from Jie Cui was not linked to WIV 162 of 163 submissions to GenBank remain suppressed or missing...with other serious data integrity issues and cyber biosecurity issues needing to be addressed... Disqualifying conflict of…

@tommy_cleary - Tommy Cleary

@_everythingism @AceBearstrom @hiltzikm STS studies regularly acknowledge and explore institutional limits to knowledge away from the political narratives you outline here. <<Undone Science Social Movements, Mobilized Publics, and Industrial Transitions By David J. Hess>> https://mitpress.mit.edu/9780262529495/undone-science/ Since this research…

Undone Science A theoretical integration of science and technology studies and social movement studies that finds both common ground and “undone” research.As the fields... mitpress.mit.edu

@CharlesRixey - Charles Rixey, MA MBA (c) 🐭

Linked below is an article written by LtCol Joseph Murphy, the person who leaked the DEFUSE proposal to me, which DRASTIC then analyzed and released on September 20th & 21st, 2021. 🧵 https://brownstone.org/articles/the-biodefense-oligarchy-and-its-demographic-defeats/

The Biodefense Oligarchy and Its Demographic Defeats ⋆ Brownstone Institute Two decades ago, factions argued that biowarfare threats were so significant that biodefense responsibility needed to be removed from the purview of the uniformed military and placed within NIAID under NIH and under HHS. brownstone.org

@tommy_cleary - Tommy Cleary

As science is very important... https://journals.asm.org/doi/10.1128/jvi.01240-24methods H/t @sciencecohen @hholdenthorp @ScienceMagazine Holmes attempted <> methods, appropriate of biological warfare investigations, with Eddie's Twitter thread on March 6th 2023, here: He was trying to demonstrate that 60 viruses submitted to GenBank as part of a 2018 preprint, together with Prof Jie Cui and ZLShi of Wuhan Institute of Virology, were complete... https://web.archive.org/web/20220809085043/https://www.ncbi.nlm.nih.gov/nuccore/?term=Spread+and+Geographic+Structure+of+SARS-related+Coronaviruses+in+++++++++++++Bats+and+the+Origin+of+Human+SARS+Coronavirus Interestingly only 163 of a potential 180 sequences, with ORF8, S & RdRp available for each, were said to be part of this 12-JUL-2018 PrePrint? Bioinformatics https://breakingdefense.com/2022/02/cyber-can-now-create-biowarfare-effects-without-a-bioweapon/ and cyberbiosecurity are important science too. But only 154 of these are in the current GI series available to be recovered... as far as I know... this thread tests these assumptions & knowledge claims...lets do some <> searching together. //Note important under examined bioinformatics data: framing this data set...with this current GI series of 154 submissions interrupted by submissions dated 25-OCT-2019 and the ACCESSION series continuing from the last, with <> to <> which is unrelated but dated Jul 13, 2019 08:18 PM. https://ncbi.nlm.nih.gov/nuccore/MH615993.1?report=girevhist This suggests that the original GenBank submission, perhaps actually of 180 sequences, was cropped to 163 and given new ACCESSION numbers one year after it was submitted...with the cropped series of 163 placed in their current GI position on 25-OCT-2019...but what of the missing 9 ORF8 from this GI series? Finding the missing data set will help demonstrate what could have happened. GI count is hypothesized as a way of delineating this Undone Science data set. /// KISS Methods: basic GI series analysis this Xpost GI is 1769824386 <> anyone can do this... even? https://ncbi.nlm.nih.gov/nuccore/1769824392 GI 1769824386 <> all good GI 1769824384 <> all good GI 1769824382 <> all good note last of the S Gene in this series GI 1769824380 <> all good GI 1769824378 <> all good GI 1769824376 <> all good GI 1769824374 <> all good GI 1769824372 <> all good GI 1769824370 <> all good GI 1769824368 <> all good GI 1769824366 <> all good GI 1769824364 <> all good GI 1769824362 <> all good GI 1769824360 <> all good GI 1769824358 <> all good GI 1769824356 <> all good GI 1769824354 <> BINGO! Finally! Again ORF8 and RdRp are here but for <> the S gene is missing. Why? So GI 1769824354 <> is the next missing data point for <> GenBank submission from @syd_health &@Sydney_UniProf Edward Holmes @EdwardCHolmes to GenBank of@NLM_NIH NOW tally is still twelve missing ORF8... and now seven missing S genes... where for <> RdRp is the there: https://www.ncbi.nlm.nih.gov/nuccore/1769824436 ORF8 gene is there but both suppressed: https://www.ncbi.nlm.nih.gov/nuccore/1769824354 but no S gene in this GI series Why? Missing ORF8 tally so far: 1) Rs151334_Guizhou 2) Rf131405_Shanxi 3) Rs140400_Guangdong 4) Rs141456_Guangxi 5) Rspp7924_Yunnan 6) Rspp7921_Yunnan 7) Ra7909_Yunnan 8) Rspp7907_Yunnan 9) Rspp7905_Yunnan 10) Rspp7896_Yunnan 11) Rs6303_Yunnan 12) Rs6266_Yunnan identified of a total of 15 missing...3 to go... Question: Was <> in the 60-54= 6 ORF8 that <> decided to leave out of this 2018 PrePrint... ...or perhaps the 54-45= 9 ORF8 that are simply missing from the GI series suppressed in GenBank & interrupted by the date 25-Oct-2019? With S genes missing too; of the 60 RdRp sampled only 49 S genes are here in this GI series... Why? 1) Rspp7921_Yunnan 2) Rspp7907_Yunnan 3) Rspp7896_Yunnan 4) Rs9214_Hubei 5) Rs9201_Hubei 6) Rs151199_Hunan 7) Rs8548_Guangdong identified of 11 S genes left out of this study...4 to go! <> S gene is missing Why? All this so far indicates that the 2023@COVIDSelectdisclosures of Holmes are potentially incomplete...but the count continues... next search is GI 1769824352! Wonder what we will find...especially when we next seek out the known duplicates in <> and <>? Duplication can mean missing, and missing mean unverified in Dual Use Research of Concern field...where one the uses is Biological Warfare and the other is fairweather thinking Science as usual? https://www.nature.com/articles/s41467-020-17687-3

Spread and Geographic Structure of SARS-related Coronaviruses in Bats - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube web.archive.org
Cyber can now create biowarfare effects, without a bioweapon - Breaking Defense The digitization of medicine and biomedical research has been a boon for medical breakthroughs, but comes at a cost. From ransomware attacks at hospitals to intellectual property breaches at research centers, cybersecurity is now a major concern in the medical world. In the following op-ed, three experts at the intersection of national security and health policy lay out the worryingly diverse ways the global healthcare system is at risk, and why it should concern the defense community.  The worst biological warfare scenarios remain in the realm of nightmares and science fiction. From developing pathogens to finding an appropriate vector, the process of weaponizing biological agents is fraught with challenges. Without discounting the well-documented history of biowarfare and the very real threat of novel weaponized biological agents in the future — particularly as gene editing and designer molecules revolutionize the field — real hurdles remain. It’s dangerous, and the effects are difficult to predict and control. But what if it was possible to create bioweapon effects, without having to actually use a bioweapon? That’s no longer a hypothetical. The digitization, automation, and networking of biomedical and public health information may mean that cyber tools can be used to achieve biowarfare effects that were previously unrealistic or impractical. Perhaps the most glaring wake-up call is the use of social media tools to spread and amplify misinformation about COVID-19 vaccines, contributing to viral illness and death of US citizens. But that’s just the tip of the iceberg when it comes to how our public health is vulnerable to direct manipulation by malicious actors in the cyber domain. 2020 saw a 200% rise in healthcare cyber-attacks, and the upward trend continues. Networked data is increasingly the backbone of our entire medical system: initial R&D/experimental biomedical research, treatment development, clinical trial data, drug supply chains, the equipment used in treatment, individual health records, and personal fitness tracking. Manipulation or theft of R&D and clinical trial data drugs, devices and treatments can invalidate results or sow doubts about their reliability, hamstringing or confounding scientific studies in response to public health crises and making people sick. The clinical R&D landscape is evolving: Growth in team-based translational science is bringing research scientists, systems thinkers, analytic boundary crossers, and business developers together across global communications architectures faster than ever. And as a result, the threat surface is growing as well. RELATED: How To Build A Better Policy For Countering WMD Threats Supply chain interference can cause widespread disruption in critical medical care or can target delivery to specific populations for more tailored effects. The sophisticated global cyber campaign targeting the COVID-19 vaccine supply chain (specifically the “cold chain”) is a striking example, but is by no means a unique event. It is part of a larger trend, in which hackers have shifted their focus in recent years to increasingly target pharmaceutical and medical supply chains. These are attractive ransomware targets for the lucrative prices they command precisely because they threaten the delivery of critical lifesaving drugs and therapies. These same supply chain vulnerabilities can be exploited by actors whose goal is not financial gain but biological damage. Hospitals and healthcare facilities are vulnerable as well. Critical life-saving machinery and devices — infusion pumps, defibrillators, ventilators, dialysis machines, and active patient monitoring devices — can be breached by both insider and external threats. Access to cyber tools can give actors the ability to disrupt, delay, or deny treatment, manipulating critical health outcomes for patients, even life or death. The ability to hold patients’ health at risk is what has made this such an appealing and profitable target for ransomware. And the COVID-19 Pandemic has shown us that these breaches are now a common occurrence. As health records and personal fitness data are increasingly specific, detailed, digitized, and shared across devices platforms, and databases, they become vulnerable. Health record breaches alone rose 300% from 2018 to 2021. Our ever-growing volume of personal health information can be harvested and even manipulated to affect specific individuals, or aggregated to target populations by race, age, gender, location, socioeconomic status, medical condition, or any number of other factors depending on the malicious actor’s goal. The blending of the biological and cyber domains suggests that we need to prepare differently for the threat of biological warfare if we are to properly defend our population. The most difficult task is changing our fundamental model of boundaries between clinical research, bio-surveillance, care delivery, and individual devices. DoD has an important leadership role to play in driving, coordinating, and overseeing this change. To start, we must embrace the same principles required by any other type of complex cyber supply chain which, according to NIST [PDF], requires that we: 1) assume our systems will be breached and consider recovery and mitigation up-front, 2) establish collaborative and cross-organizational governance organized by use case with clinical and business owners at the forefront, backed by security experts, and 3) remember that a risk anywhere in the entire chain can impact any link — it may not be your responsibility contractually, but it will be your problem in reality. In the clinical cyber supply chain, the individual software systems receive most of the focus, but it is the rapidly changing interconnections where breaches happen most often — so working together to adjust perceived systems boundaries and overall mental models must be a continual task. The community of interest – which includes scientists, pharmaceutical companies, medical technology developers and manufacturers, academics, cyber security professionals, national defense professionals, and patients – is far-reaching, fragmented, and stove-piped. We must undertake a holistic reevaluation of biological warfare defense in the context of a changing and networked public health ecosystem. Katherine Hasty is a US Air Force veteran and director of Future Warfare at Long Term Strategy Group. Dr. Janie L. Gittleman is executive director for Global Health Innovation at ManTech International and a former Senior Health Advisor to the Defense Intelligence Agency Surgeon General. Edward F. O’Connor is a Subject Matter Expert with ManTech’s Health Division and a former CIO of Central Health and the Community Care Collaborative.   breakingdefense.com
Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs151239_Hunan ORF8 gene, complete cd - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs8548_Guangdong RNA-dependent RNA po - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs8548_Guangdong ORF8 gene, complete - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
RETRACTED ARTICLE: Origin and cross-species transmission of bat coronaviruses in China - Nature Communications Bats are presumed reservoirs of diverse coronaviruses (CoVs) including progenitors of Severe Acute Respiratory Syndrome (SARS)-CoV and SARS-CoV-2, the causative agent of COVID-19. However, the evolution and diversification of these coronaviruses remains poorly understood. Here we use a Bayesian statistical framework and a large sequence data set from bat-CoVs (including 630 novel CoV sequences) in China to study their macroevolution, cross-species transmission and dispersal. We find that host-switching occurs more frequently and across more distantly related host taxa in alpha- than beta-CoVs, and is more highly constrained by phylogenetic distance for beta-CoVs. We show that inter-family and -genus switching is most common in Rhinolophidae and the genus Rhinolophus. Our analyses identify the host taxa and geographic regions that define hotspots of CoV evolutionary diversity in China that could help target bat-CoV discovery for proactive zoonotic disease surveillance. Finally, we present a phylogenetic analysis suggesting a likely origin for SARS-CoV-2 in Rhinolophus spp. bats. Bats are a likely reservoir of zoonotic coronaviruses (CoVs). Here, analyzing bat CoV sequences in China, the authors find that alpha-CoVs have switched hosts more frequently than betaCoVs, identify a bat family and genus that are highly involved in host-switching, and define hotspots of CoV evolutionary diversity. nature.com

@tommy_cleary - Tommy Cleary

@R_H_Ebright @alisonannyoung With ongoing cyberbiosecurity issues the whole time! The problem of knowledge silos within and between cybersecurity and bio world continues throughout this period from 2008 to NOW! Still now… Why?

@tommy_cleary - Tommy Cleary

@R_H_Ebright @ScienceMagazine Further...as much as Holmes has stated that data from Jie Cui was not linked to WIV 162 of 163 submissions to GenBank remain suppressed or missing...with other serious data integrity issues and cyber biosecurity issues needing to be addressed... Disqualifying conflict of…

@tommy_cleary - Tommy Cleary

@_everythingism @AceBearstrom @hiltzikm STS studies regularly acknowledge and explore institutional limits to knowledge away from the political narratives you outline here. <<Undone Science Social Movements, Mobilized Publics, and Industrial Transitions By David J. Hess>> https://mitpress.mit.edu/9780262529495/undone-science/ Since this research…

Undone Science A theoretical integration of science and technology studies and social movement studies that finds both common ground and “undone” research.As the fields... mitpress.mit.edu

@tommy_cleary - Tommy Cleary

My application for SAGO at @WHO was rejected...but it was in volunteer capacity and so I simply continued to help where I can. https://2012-2017.usaid.gov/sites/default/files/documents/2496/Combatting_Corruption_Among_Civil_Servants_-_Interdisciplinary_Perspectives_on_What_Works.pdf My skill sets are listening...catching...and surprise...not simplicity H/t @CharlesRixey Umberto Eco said it well. If it is too complicated, read more books. But he wrote this type of thing in Italian, so don't see these ideas as complexity, see them as language. Teaching a language takes time and repetition...about two years of immersion...or you can nowadays Gronk your way through? The lived experience here is of an INCOMPLETE data set...so obviously I cannot fully explain the data...but you can join me on the journey. Surprise! Truth is important...but it takes a lot of listening to hear certain truths...trauma adds more layers of humanity and so our souls are stretched thinly as we listen to the person within the cyborg of text based embodiment twisting under the weight of the unknown...but knowable: <> LtCol Joe Murphy US Marines https://brownstone.org/author/joe-murphy/ In this space and habits of removed and gone...Holmes blinked and attempted <> methods, appropriate to biological warfare investigations, with Eddie's Twitter thread on March 6th 2023, here: Why? Good question, ask him. He says he was trying to demonstrate that 60 viruses submitted to GenBank as part of a 2018 preprint, together with Prof Jie Cui and ZLShi of Wuhan Institute of Virology, were complete... https://web.archive.org/web/20220809085043/https://www.ncbi.nlm.nih.gov/nuccore/?term=Spread+and+Geographic+Structure+of+SARS-related+Coronaviruses+in+++++++++++++Bats+and+the+Origin+of+Human+SARS+Coronavirus Interestingly only 163 of a potential 180 sequences, with ORF8, S & RdRp available for each, were said to be part of this 12-JUL-2018 PrePrint? But only 154 of these are in the current GI series available to be recovered... as far as I know...and I don't know everything...I am seeking the answers to fairly obvious questions. This thread tests these assumptions & knowledge claims... So lets do some <> searching together! // Forensic note: important under examined bioinformatics data is framing this data set...with this current GI series of 154 submissions interrupted by submissions dated 25-OCT-2019 and the ACCESSION series continuing from the last, with <> to <> which is unrelated but dated Jul 13, 2019 08:18 PM. https://ncbi.nlm.nih.gov/nuccore/MH615993.1?report=girevhist This suggests that the original earlier GenBank submission, perhaps actually of 180 sequences, was cropped to 163 and given new ACCESSION numbers one year after it was submitted...with the cropped series of 163 placed in their current GI position on 25-OCT-2019...but what of the missing 9 ORF8 from this GI series? Finding the missing data set will help demonstrate what could have happened. GI count is hypothesized as a way of delineating this Undone Science data set. /// KISS Methods: basic GI series analysis this Xpost GI is 1769824352 <> anyone can do this... even you? But if you cannot, what does this say about how easy it is to make a mistake in a DURC program? https://ncbi.nlm.nih.gov/nuccore/1769824352 GI 1769824352 <> all good, all three, ORF8, RdRp and S genes present. https://ncbi.nlm.nih.gov/nuccore/MH615857.1?report=genbank GI 1769824350 <> all good too https://ncbi.nlm.nih.gov/nuccore/MH615856.1?report=genbank GI 1769824348 <> all good https://ncbi.nlm.nih.gov/nuccore/MH615855.1?report=genbank GI 1769824346 <> all good https://ncbi.nlm.nih.gov/nuccore/MH615854.1?report=genbank GI 1769824344 <> all good https://ncbi.nlm.nih.gov/nuccore/MH615853.1?report=girevhist GI 1769824342 <> all good https://ncbi.nlm.nih.gov/nuccore/MH615852.1?report=genbank GI 1769824340 <> all good https://ncbi.nlm.nih.gov/nuccore/MH615851.1?report=genbank GI 1769824338 <> all good https://ncbi.nlm.nih.gov/nuccore/MH615850.1?report=genbank GI 1769824336 <> all good https://ncbi.nlm.nih.gov/nuccore/MH615849.1?report=genbank GI 1769824334 <> all good https://ncbi.nlm.nih.gov/nuccore/MH615848.1?report=genbank GI 1769824332 <> all good https://ncbi.nlm.nih.gov/nuccore/MH615847.1?report=genbank GI 1769824330 <> all good https://ncbi.nlm.nih.gov/nuccore/MH615846.1?report=genbank GI 1769824328 <> all good https://ncbi.nlm.nih.gov/nuccore/MH615845.1?report=genbank GI 1769824326 <> all good https://ncbi.nlm.nih.gov/nuccore/MH615844.1?report=genbank GI 1769824324 <> BINGO!!!! @MonaRahalkar your old friend! Finally! Again ORF8 and RdRp are here but for <> the S gene is missing. Why? So GI 1769824324 <> is the next missing data point for <> GenBank submission from@syd_health&@Sydney_UniProf Edward Holmes @EdwardCHolmes to GenBank of@NLM_NIH NOW tally is still twelve missing ORF8... and now eight missing S genes... where for <> RdRp is the there in two naming versions but only partly suppressed here: https://www.ncbi.nlm.nih.gov/nuccore/1769824434 and here https://www.ncbi.nlm.nih.gov/nuccore/MH615898.1?report=girevhist but not available to GenBank search terms: <> https://ncbi.nlm.nih.gov/nuccore/?term=Spread+and+Geographic+Structure+of+SARS-related+Coronaviruses+in+Bats+and+the+Origin+of+Human+SARS+Coronavirus or <> https://ncbi.nlm.nih.gov/nuccore/?term=Yu%2CP.%2C+Hu%2CB.%2C+Li%2CB.%2C+Luo%2CD.%2C+Zhu%2CG.%2C+Zhang%2CL.%2C+Holmes%2CE.C.%2C+Shi%2CZ.+and+Cui%2CJ. Strange isn't it? ORF8 gene is there again with two names but both searches for title and authors are not available again: https://ncbi.nlm.nih.gov/nuccore/1769824324 &here https://www.ncbi.nlm.nih.gov/nuccore/MH615843.1?report=girevhist If the <> linked submissions are not suppressed then these search terms should give at least two results for the ORF8 and RpRd? But in any case no S gene in this GI series for <> Why? Recap: Missing ORF8 tally so far: 1) Rs151334_Guizhou 2) Rf131405_Shanxi 3) Rs140400_Guangdong 4) Rs141456_Guangxi 5) Rspp7924_Yunnan 6) Rspp7921_Yunnan 7) Ra7909_Yunnan 8) Rspp7907_Yunnan 9) Rspp7905_Yunnan 10) Rspp7896_Yunnan 11) Rs6303_Yunnan 12) Rs6266_Yunnan identified of a total of 15 missing...3 to go... Question: Was <> in the 60-54= 6 ORF8 that <> decided to leave out of this 2018 PrePrint... ...or perhaps the 54-45= 9 ORF8 that are simply missing from the GI series suppressed in GenBank & interrupted by the date 25-Oct-2019? With S genes missing too; of the 60 RdRp sampled only 49 S genes are here in this GI series... Why? 1) Rspp7921_Yunnan 2) Rspp7907_Yunnan 3) Rspp7896_Yunnan 4) Rs9214_Hubei 5) Rs9201_Hubei 6) Rs151199_Hunan 7) Rs8548_Guangdong 8) RaTG13_Yunnan//Ra4991_Yunnan identified of 11 S genes left out of this study...3 to go! <> S gene is missing yet it is very important...especially the version of Ra4991 that was originally loaded on to GenBank before this current GI series was perhaps placed, cropped, edited and moved and given new ACCESSION codes. This apparently happened from Jul 13, 2019 08:18 PM to 25-OCT-2019 So <> methodology requires more data. All this so far indicates that the 2023 disclosures of Holmes are potentially incomplete...but the count continues... next search is GI 1769824322! How to make strong knowledge claims about the origin of COVID without these data sets? Well you cannot. But Holmes gives it a go. @GrahamPerrettMP ? Any word from the relevant Ministers yet? https://www.sydney.edu.au/infectious-diseases-institute/news-and-events/news/2020/03/24/the-proximal-origin-of-sars-cov-2.html

Archive - U.S. Agency for International Development 2012-2017.usaid.gov
Joe Murphy, Author at Brownstone Institute Joe Murphy is a lieutenant colonel in the US Marines with 16+ years of service. brownstone.org
Spread and Geographic Structure of SARS-related Coronaviruses in Bats - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube web.archive.org
Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs8460_Guangdong ORF8 gene, complete - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs8460_Guangdong ORF8 gene, complete - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs8363_Guangdong ORF8 gene, complete - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs151569_Guizhou ORF8 gene, complete - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs151514_Guizhou ORF8 gene, complete - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs151493_Guizhou ORF8 gene, complete - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs151491_Guizhou ORF8 gene, complete - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs151388_Guizhou ORF8 gene, complete - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs151262_Guizhou ORF8 gene, complete - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs141567_Guangxi ORF8 gene, complete - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs141455_Guangxi ORF8 gene, complete - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs13488_Guangxi ORF8 gene, complete c - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs13484_Guangxi ORF8 gene, complete c - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs13479_Guangxi ORF8 gene, complete c - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Bat SARS-like coronavirus strain RaTG13_Yunnan RNA-dependent RNA polym - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
No items found - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
No items found - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Bat SARS-like coronavirus strain RaTG13_Yunnan ORF8 gene, complete cds - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
The proximal origin of SARS-CoV-2 sydney.edu.au

@tommy_cleary - Tommy Cleary

@JamieMetzl @WHO I applied @WHO SAGO but didnt get in... so continued with thesis from OSINT epidemiology perspective as type of study that @mvankerkhove et al are probably not able to perform in an institutionally independent way...hope it helps.

@tommy_cleary - Tommy Cleary

@R_H_Ebright @ScienceMagazine Further...as much as Holmes has stated that data from Jie Cui was not linked to WIV 162 of 163 submissions to GenBank remain suppressed or missing...with other serious data integrity issues and cyber biosecurity issues needing to be addressed... Disqualifying conflict of…

@tommy_cleary - Tommy Cleary

@_everythingism @AceBearstrom @hiltzikm STS studies regularly acknowledge and explore institutional limits to knowledge away from the political narratives you outline here. <<Undone Science Social Movements, Mobilized Publics, and Industrial Transitions By David J. Hess>> https://mitpress.mit.edu/9780262529495/undone-science/ Since this research…

Undone Science A theoretical integration of science and technology studies and social movement studies that finds both common ground and “undone” research.As the fields... mitpress.mit.edu

@tommy_cleary - Tommy Cleary

<> methods, appropriate to biological warfare investigations, with Eddie's Twitter thread on March 6th 2023, here: Why? Good question, ask him. @sciencecohen <> Yep...my guess is that Jon knew about the RaTG13/Ra4991 from sick miners but decided not to or was directed not to say anything right away. H/t @R_H_Ebright 5 years ago after being on the case for 25 years... Holmes says he was trying to demonstrate that 60 viruses submitted to GenBank as part of a 2018 preprint, together with Prof Jie Cui and ZLShi of Wuhan Institute of Virology, were complete...but they are obviously incomplete. https://web.archive.org/web/20220809085043/https://www.ncbi.nlm.nih.gov/nuccore/?term=Spread+and+Geographic+Structure+of+SARS-related+Coronaviruses+in+++++++++++++Bats+and+the+Origin+of+Human+SARS+Coronavirus Only 163 of a potential 180 sequences, with ORF8, S & RdRp available for each, were said to be part of this 12-JUL-2018 PrePrint? But bioinformatics analysis is important to these knowledge claims, H/t Trevor Bedford @trvrbonly ...and only 154 of these are in the current GenBank GI series available to be recovered...as far as I know...and I don't know everything...I am seeking the answers to fairly obvious questions...like why were these 180 GenBank submissions not available when WIV frist published post COVID outbreak discovery? https://www.biorxiv.org/content/10.1101/2020.01.22.914952v2.full.pdf This thread tests these assumptions & knowledge claims... So lets do some <> bioinformatics philosophy of science searching together! // Important Forensic note: important under examined bioinformatics data is framing this data set...with this current GI series of 154 submissions interrupted by submissions dated 25-OCT-2019 and the ACCESSION series continuing from the last, with <> to <> which is unrelated but dated Jul 13, 2019 08:18 PM. https://ncbi.nlm.nih.gov/nuccore/MH615993.1?report=girevhist This suggests that the original earlier GenBank submission, perhaps actually of 180 sequences, was cropped to 163 and given new ACCESSION numbers one year after it was submitted...with the cropped series of 163 placed in their current GI position on 25-OCT-2019...but what of the missing 9 ORF8 from this GI series? Finding the missing data set will help demonstrate what could have happened. GI count is hypothesized as a way of delineating this Undone Science data set. /// KISS Methods: basic GI series analysis this Xpost thread GI is next after 1769824324 <> anyone can do this... even you? https://ncbi.nlm.nih.gov/nuccore/1769824352 GI 1769824322 <> all good, all three, ORF8, RdRp and S genes present. https://www.ncbi.nlm.nih.gov/nuccore/MH615842.1?report=genbank GI 1769824320 <> all good too https://www.ncbi.nlm.nih.gov/nuccore/MH615842.1?report=genbank GI 1769824318 <> all good https://www.ncbi.nlm.nih.gov/nuccore/MH615840.1?report=genbank GI 1769824316 <> all good but remember that the full sequence of Rs5725_Yunnan was available for the thesis <> of WIV but for <> only the ORF8, RdRp & S gene were available. https://www.ncbi.nlm.nih.gov/nuccore/MH615839.1?report=genbank Remember that GI 1769824315 is where this GI series ends with <> Submitted (25-JUL-2018) and placed Oct 25, 2019 06:16 PM together with this GI series? https://www.ncbi.nlm.nih.gov/nuccore/1769824315 Finally! NOW tally is still twelve missing ORF8... and now eight missing S genes... where for <> RdRp is the last to be found with this GI count there in two naming versions but only partly suppressed here: https://ncbi.nlm.nih.gov/nuccore/1769824434and here https://ncbi.nlm.nih.gov/nuccore/MH615898.1?report=girevhistbut not available to GenBank search terms: <> https://ncbi.nlm.nih.gov/nuccore/?term=Spread+and+Geographic+Structure+of+SARS-related+Coronaviruses+in+Bats+and+the+Origin+of+Human+SARS+Coronavirusor <> https://ncbi.nlm.nih.gov/nuccore/?term=Yu%2CP.%2C+Hu%2CB.%2C+Li%2CB.%2C+Luo%2CD.%2C+Zhu%2CG.%2C+Zhang%2CL.%2C+Holmes%2CE.C.%2C+Shi%2CZ.+and+Cui%2CJ. Strange isn't it? ORF8 gene is there again with two names but both searches for title and authors are not available again: https://ncbi.nlm.nih.gov/nuccore/1769824324 &here https://ncbi.nlm.nih.gov/nuccore/MH615843.1?report=girevhist If the <> linked submissions are not suppressed then these search terms should give at least two results for the ORF8 and RpRd? But in any case no S gene in this GI series for <> Why? Recap: Missing ORF8 tally so far: 1) Rs151334_Guizhou 2) Rf131405_Shanxi 3) Rs140400_Guangdong 4) Rs141456_Guangxi 5) Rspp7924_Yunnan 6) Rspp7921_Yunnan 7) Ra7909_Yunnan 8) Rspp7907_Yunnan 9) Rspp7905_Yunnan 10) Rspp7896_Yunnan 11) Rs6303_Yunnan 12) Rs6266_Yunnan identified of a total of 15 missing...3 to go... Question: Was <> in the 60-54= 6 ORF8 that <> decided to leave out of this 2018 PrePrint... ...or perhaps the 54-45= 9 ORF8 that are simply missing from the GI series suppressed in GenBank & interrupted by the date 25-Oct-2019? With S genes missing too; of the 60 RdRp sampled only 49 S genes are here in this GI series... Why? 1) Rspp7921_Yunnan 2) Rspp7907_Yunnan 3) Rspp7896_Yunnan 4) Rs9214_Hubei 5) Rs9201_Hubei 6) Rs151199_Hunan 7) Rs8548_Guangdong 8) RaTG13_Yunnan//Ra4991_Yunnan identified of 11 S genes left out of this study...3 to go! <> S gene is missing yet it is very important...especially the version of Ra4991 that was originally loaded on to GenBank before this current GI series was perhaps placed, cropped, edited and moved and given new ACCESSION codes. This apparently happened from Jul 13, 2019 08:18 PM to 25-OCT-2019 So <> methodology requires more data. All this so far indicates that the 2023 disclosures of Holmes are potentially incomplete... How to make strong knowledge claims about the origin of COVID without these data sets? Well you cannot. But Holmes gives it a go. To find the rest of the missing data points we need to examine the 180 potential for the 3 S and 3 OFRF8 missing. It is so easy to make mistakes with this type of count and so checking and rechecking with different methodologies is important. This is the complex ground of the information domain. I have to back track and see if I have missed a thread in the GI series? This is why I have left this trail of pebbles...so I can back track when needed. https://brownstone.org/articles/the-biodefense-oligarchy-and-its-demographic-defeats/

Spread and Geographic Structure of SARS-related Coronaviruses in Bats - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube web.archive.org
Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs8460_Guangdong ORF8 gene, complete - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs160665_Yunnan ORF8 gene, complete c - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs160665_Yunnan ORF8 gene, complete c - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rf5511_Yunnan ORF8 gene, complete cds - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs5725_Yunnan ORF8 gene, complete cds - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Salmonella enterica subsp. enterica serovar Infantis strain FSIS170229 - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
No items found - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Bat SARS-like coronavirus strain RaTG13_Yunnan RNA-dependent RNA polym - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
No items found - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
No items found - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Bat SARS-like coronavirus strain RaTG13_Yunnan ORF8 gene, complete cds - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
The Biodefense Oligarchy and Its Demographic Defeats ⋆ Brownstone Institute Two decades ago, factions argued that biowarfare threats were so significant that biodefense responsibility needed to be removed from the purview of the uniformed military and placed within NIAID under NIH and under HHS. brownstone.org

@R_H_Ebright - Richard H. Ebright

Five years ago today, a scientist stated publicly that data were consistent with a lab origin: "Ebright tells Science...that the 2019-nCoV data are 'consistent with entry into the human population as either a natural accident or a laboratory accident.'" https://www.science.org/content/article/mining-coronavirus-genomes-clues-outbreak-s-origins

@tommy_cleary - Tommy Cleary

@R_H_Ebright @ScienceMagazine Further...as much as Holmes has stated that data from Jie Cui was not linked to WIV 162 of 163 submissions to GenBank remain suppressed or missing...with other serious data integrity issues and cyber biosecurity issues needing to be addressed... Disqualifying conflict of…

@tommy_cleary - Tommy Cleary

@_everythingism @AceBearstrom @hiltzikm STS studies regularly acknowledge and explore institutional limits to knowledge away from the political narratives you outline here. <<Undone Science Social Movements, Mobilized Publics, and Industrial Transitions By David J. Hess>> https://mitpress.mit.edu/9780262529495/undone-science/ Since this research…

Undone Science A theoretical integration of science and technology studies and social movement studies that finds both common ground and “undone” research.As the fields... mitpress.mit.edu

@tommy_cleary - Tommy Cleary

This is where I lost count! Doh! Next GI will have to start from here and be inserted into current tally. GI 1769824546 restart count again here...and insert missing into tally KISS Methods: basic GI series analysis this Xpost GI is 1769824546 <> anyone can do this... even you? But if you cannot, or if you lose count so easily, like I always do...what does this say about how easy it is to make a mistake in a DURC program? https://ncbi.nlm.nih.gov/nuccore/1769824546 GI 1769824546 <> all good, all three, ORF8, RdRp and S genes present. Next GI 1769824544 <> & RdRp are there but suppressed but ORF8 is already 5) on the tally https://www.ncbi.nlm.nih.gov/nuccore/MH615953.1?report=genbank GI 1769824542 <> & RdRp are there but suppressed but ORF8 is should be 6) on the tally not 7) as I must have started counting GI from the RdRp list here? https://www.ncbi.nlm.nih.gov/nuccore/MH615952.1?report=genbank GI 1769824540 <> & RdRp are there but should be 7) on the list not 9)? https://www.ncbi.nlm.nih.gov/nuccore/MH615951.1?report=genbank GI 1769824538 <> & RdRp are there but should be 8) and is missing! https://www.ncbi.nlm.nih.gov/nuccore/MH615951.1?report=genbank Lets keep going to the next one... GI 1769824536 <> & RdRp & ORF8 are there https://www.ncbi.nlm.nih.gov/nuccore/MH615949.1?report=genbank GI 1769824534 <> & RdRp & ORF8 are there https://www.ncbi.nlm.nih.gov/nuccore/1769824534 GI 1769824532 <> & RdRp but missing ORF8 should be 9) on the list not 12) https://www.ncbi.nlm.nih.gov/nuccore/1769824532 GI 1769824530 <> & RdRp & ORF8 are there all good https://www.ncbi.nlm.nih.gov/nuccore/1769824530 GI 1769824528 <> & RdRp but ORF8 missing should be 10) on tally not 11) https://www.ncbi.nlm.nih.gov/nuccore/1769824528 GI 1769824526 <> & RdRp & ORF8 all good https://www.ncbi.nlm.nih.gov/nuccore/1769824526 GI 1769824524 is <> so S & RdRp & ORF8 all good https://www.ncbi.nlm.nih.gov/nuccore/1769824524 GI 1769824522 is <> so S & RdRp & ORF8 all good https://www.ncbi.nlm.nih.gov/nuccore/1769824522 GI 1769824520 is <> all good GI 1769824518 is <> all good GI 1769824516 is <> all good GI 1769824514 is <> all good GI 1769824512 is <> all good GI 1769824510 is <> ORF8 missing 1) on tally GI 1769824508 is <> all good GI 1769824506 is <> all good GI 1769824504 is <> all good GI 1769824502 is <> all good GI 1769824500 is <> is tricky missing S gene 1) in tally not 4) missing but RdRp and ORF8 OK https://www.ncbi.nlm.nih.gov/nuccore/MH615936.1?report=genbank GI 1769824498 is <> again missing S gene 2) not 5) in tally, but RdRp & ORF8 are good. https://www.ncbi.nlm.nih.gov/nuccore/MH615930.1?report=genbank GI 1769824496 is <> again missing S gene 3) not 6) in tally, but RdRp & ORF8 are good. https://www.ncbi.nlm.nih.gov/nuccore/MH615930.1?report=genbank GI 1769824494 is <> all good GI 1769824492 is <> ORF8 is missing 2) in tally GI 1769824490 is <> all good GI 1769824488 is <> all good GI 1769824486 is <> all good GI 1769824484 is <> all good GI 1769824482 is <> dare I say BINGO!!! <> RdRp is there https://www.ncbi.nlm.nih.gov/nuccore/MH615922.1?report=girevhist but ORF8 is missing number 11) and S is missing number 4) NOW tally is still fourteen missing ORF8... and still nine missing S genes... Recap: Missing ORF8 tally so far with order fixed: 1) Rs151334_Guizhou 2) Rf131405_Shanxi 3) Rs140400_Guangdong 4) Rs141456_Guangxi 5) Rspp7924_Yunnan 6) Ra7909_Yunnan prev Rspp7921_Yunnan 7) Rspp7905_Yunnan prev Ra7909_Yunnan 8) Rspp7931_Yunnan prev Rspp7907_Yunnan 9) Rs6266_Yunnan prev Rspp7905_Yunnan 10) Rs6303_Yunnan prev Rspp7896_Yunnan 11) Rf130223-29_Beijing prev Rs6303_Yunnan 12) Rs6266_Yunnan identified of a total of 15 missing...1 to go? Question: Was <> in the 60-54= 6 ORF8 that <> decided to leave out of this 2018 PrePrint... ...or perhaps the 54-45= 9 ORF8 that are simply missing from the GI series suppressed in GenBank & interrupted by the date 25-Oct-2019? With S genes missing too; of the 60 RdRp sampled only 49 S genes are here in this GI series... Why? 1) Rs9214_Hubei prev Rspp7921_Yunnan 2) Rs9201_Hubei prev Rspp7907_Yunnan 3) Rs151199_Hunan prev Rspp7896_Yunnan 4) Rf130223-29_Beijing prev Rs9214_Hubei 5) Rs9201_Hubei 6) Rs151199_Hunan 7) Rs8548_Guangdong 8) RaTG13_Yunnan//Ra4991_Yunnan identified of 11/11 S genes left out of this study... <> S gene is missing yet it is very important...especially the version of Ra4991 that was originally loaded on to GenBank before this current GI series was perhaps placed, cropped, edited and moved and given new ACCESSION codes. This apparently happened from Jul 13, 2019 08:18 PM to 25-OCT-2019 So <> methodology requires more data. All this so far indicates that the 2023 disclosures of Holmes are potentially incomplete...but the count continues... next search is GI 1769824322! How to make strong knowledge claims about the origin of COVID without these data sets? Well you cannot. This tally is nice and messy at the moment...I will need to clean it up in the next post! Counting from GI 1769824482 <> and smoothing out the tally. A stuff up in a GI count like this is character building, but also gives an insight into the lived experience of bioinformatics of Virology. How could this type of thing but constantly happening an STILL there is an attitude that errors are not common. What bullshit! They happen all the time! Like @sciencecohen who details DNA of SARS-CoV-2 instead of RNA. We are all people doing people stuff...stuff-ups too. https://www.science.org/content/article/mining-coronavirus-genomes-clues-outbreak-s-origins

Record suppressed: Bat SARS-like coronavirus strain Rs8363_Guangdong spike protein (S) ge - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rspp7924_Yunnan spike protein (S) gen - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Ra7909_Yunnan spike protein (S) gene, - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rspp7905_Yunnan spike protein (S) gen - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rspp7905_Yunnan spike protein (S) gen - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs5725_Yunnan spike protein (S) gene, - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs160665_Yunnan spike protein (S) gen - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs6266_Yunnan spike protein (S) gene, - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs6255_Yunnan spike protein (S) gene, - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs6303_Yunnan spike protein (S) gene, - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rf5511_Yunnan spike protein (S) gene, - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs161465_Guangdong RNA-dependent RNA - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs161419_Guangdong RNA-dependent RNA - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs151334_Guizhou RNA-dependent RNA po - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs9201_Hubei RNA-dependent RNA polyme - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs9201_Hubei RNA-dependent RNA polyme - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov

@tommy_cleary - Tommy Cleary

Next one? <> no ORF8 found in @NLM_NIH GenBank <>, complete cds is there but suppressed... GenBank: MH615955.1 https://www.ncbi.nlm.nih.gov/nuccore/MH615955.1?report=genbank <> GenBank: MH615905.1 is there but suppressed... https://www.ncbi.nlm.nih.gov/nuccore/MH615905.1?report=genbank ...so that is NOW four missing ORF8; all with S and RdRp available but suppressed; 1) Rs151334_Guizhou 2) Rf131405_Shanxi 3) Rs140400_Guangdong 4) Rs141456_Guangxi Were these in the 60-54=6 ORF8 that <> decided to leave out of this PrePrint ? https://web.archive.org/web/20220809085043/https://www.ncbi.nlm.nih.gov/nuccore/?term=Spread+and+Geographic+Structure+of+SARS-related+Coronaviruses+in+++++++++++++Bats+and+the+Origin+of+Human+SARS+Coronavirus ...or perhaps the 54-45= 9 ORF8 that are simply missing from the GI series in GenBank? https://news.clearancejobs.com/2019/08/15/weaponizing-medicine-chinas-latest-theft-a-potential-biological-weapon/ Who knows? @COVIDSelect @COVIDSelectDems ? @R_H_Ebright

Record suppressed: Bat SARS-like coronavirus strain Rs141456_Guangxi spike protein (S) ge - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rs141456_Guangxi RNA-dependent RNA po - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Spread and Geographic Structure of SARS-related Coronaviruses in Bats - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube web.archive.org
Weaponizing Medicine: China's Latest Theft a Potential Biological Weapon A Canadian research lab sent deadly virus strains to China under the guise of scientific advancement. Now a Chinese lab scientist has been dismissed and Canadian law enforcement investigates. - Intelligence news.clearancejobs.com

@tommy_cleary - Tommy Cleary

Censorship of the nature deployed in the case of COVID had some obvious negative effects...but some were not so bad. @BiosafetyNow https://biosafetynow.substack.com/p/censoring-virology It was nice and quiet. The people censored had to find ways to reach out to each other...the phenomenology was that we had to look at what we were looking through. It also builds a compassion for a data set you are auditing during verification and for your own findings...a health doubt...need to double check and have peers that are brutal not lazy. Fixing this mess is going to be fun! Some wisdom always comes from a moment of stupidity and reflection. KISS Methods: basic GI series analysis this Xpost GI 1769824482 <> anyone can do this... even you? But if you cannot, or if you lose count so easily, like I always do...what does this say about how easy it is to make a mistake in a DURC program? https://ncbi.nlm.nih.gov/nuccore/1769824482 Starting with next RdRp GI 1769824480 is <> all good GI 1769824478 is <> S gene misssssing BBBBBingo! S gene missing no 5) in tally more BLAST homework here https://www.ncbi.nlm.nih.gov/nuccore/MH615920.1?report=genbank Couple more to find! GI 1769824476 <> all good GI 1769824474 <> all good GI 1769824472 <> all good GI 1769824470 <> all good GI 1769824468 <> all good GI 1769824466 <> all good GI 1769824464 <> all good GI 1769824462 <> ORF8 missing number 3) GI 1769824460 <> all good GI 1769824458 <> all good GI 1769824456 <> all good GI 1769824454 <> all good GI 1769824452 <> all good GI 1769824450 <> all good GI 1769824448 <> missing ORF8 tally number 4) GI 1769824446 <> all good GI 1769824444 <> all good GI 1769824442 <> all good GI 1769824440 <> all good GI 1769824438 <> all good GI 1769824436 <> missing S number 6) not 7) GI 1769824434 <> missing S number 7) prev 8) GI 1769824432 <> Binnnngooooo RdRp good, https://www.ncbi.nlm.nih.gov/nuccore/MH615897.1?report=genbank missing S number 7) & ORF8 missing number 12) with complete genome available on CNCB from June 2021 https://ngdc.cncb.ac.cn/biosample/browse/SAMC346732 NOW tally is still a mess Recap: Missing ORF8 tally so far with order fixed: 1) Rs151334_Guizhou 2) Rf131405_Shanxi 3) Rs140400_Guangdong 4) Rs141456_Guangxi 5) Rspp7924_Yunnan 6) Ra7909_Yunnan prev Rspp7921_Yunnan 7) Rspp7905_Yunnan prev Ra7909_Yunnan 8) Rspp7931_Yunnan prev Rspp7907_Yunnan 9) Rs6266_Yunnan prev Rspp7905_Yunnan 10) Rs6303_Yunnan prev Rspp7896_Yunnan 11) Rf130223-29_Beijing prev Rs6303_Yunnan 12) Rspp7952_Yunnan prev Rs6266_Yunnan 13) 14) 15) identified of a total of 15 missing Question: Was <> in the 60-54= 6 ORF8 that <> decided to leave out of this 2018 PrePrint... ...or perhaps the 54-45= 9 ORF8 that are simply missing from the GI series suppressed in GenBank & interrupted by the date 25-Oct-2019? With S genes missing too; of the 60 RdRp sampled only 49 S genes are here in this GI series... Why? 1) Rs9214_Hubei prev Rspp7921_Yunnan 2) Rs9201_Hubei prev Rspp7907_Yunnan 3) Rs151199_Hunan prev Rspp7896_Yunnan 4) Rf130223-29_Beijing prev Rs9214_Hubei 5) Rp8794_Guangdong prev Rs9201_Hubei 6) Rs8548_Guangdong prev Rs151199_Hunan 7) RaTG13_Yunnan RNA-dependent prev Rs8548_Guangdong 8) Rspp7952_Yunnan prev RaTG13_Yunnan//Ra4991_Yunnan 9) 10) 11) identified of 11 S genes left out of this study... <> S gene is missing So <> methodology requires more data. All this so far indicates that the 2023 disclosures of Holmes are potentially incomplete...but the count continues... next search is from GI 1769824432! How to make strong knowledge claims about the origin of COVID without these data sets? Well you cannot. This tally is nice and messy at the moment...I will need to continue to clean it up in the next post! Counting from GI 1769824432 <> and smoothing out the tally. Eventually it may be clearer than bee shit https://www.cia.gov/readingroom/document/cia-rdp90-00965r000403600002-0

Censoring virology "On reading," by Simon Wain-Hobson, is a weekly discussion of scientific papers and news articles around gain of function research in virology. biosafetynow.substack.com
Record suppressed: Bat SARS-like coronavirus strain Rf130223-29_Beijing RNA-dependent RNA - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rp8794_Guangdong RNA-dependent RNA po - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Record suppressed: Bat SARS-like coronavirus strain Rspp7952_Yunnan RNA-dependent RNA pol - Nucleotide - NCBITwitterFacebookLinkedInGitHubNCBI Insights BlogTwitterFacebookYoutube ncbi.nlm.nih.gov
Browse - BioSample - CNCB-NGDC ngdc.cncb.ac.cn
THE 'BEE FECES' THEORY UNDONE | CIA FOIA (foia.cia.gov) cia.gov

@a_kruschke - A.Kruschke

@tommy_cleary @JAHawk94684 @MartinaSisters @mlperk1 @MonaRahalkar @R_H_Ebright @quay_dr @BiosafetyNow @syd_health @SystemsVirology @NLM_NIH @POTUS @Sydney_Uni @DrJBhattacharya @FloDebarre @institutpasteur @thackerpd @Rebecca21951651 @capitolsheila @BillyBostickson @breakfast_dogs @Globalbiosec @gdemaneuf @RdeMaistre @dasher8090 @COVIDSelect @GrahamPerrettMP @harishseshadri2 @emilyakopp @Ayjchan @VBruttel @CharlesRixey @sciencecohen @hholdenthorp @ScienceMagazine @WHO @reSeeIt save Thread

Saved - June 17, 2025 at 2:17 AM
reSee.it AI Summary
I express deep concerns about Gain-of-Function (GoF) research, arguing it has not produced any viable vaccines or therapeutics. Despite numerous studies, no effective prevention measures have emerged, and existing vaccines derive from circulating strains, not GoF work. I highlight the dangers of this research, suggesting it may contribute to pandemics rather than prevent them. Additionally, I criticize the lack of accountability in labs following leaks and the manipulation of data by authoritarian regimes, drawing parallels to historical cover-ups. Trust in such systems is fundamentally compromised.

@KevinMcCairnPhD - Kevin W. McCairn PhD

https://bmj.com/content/344/bmj.e2398/rapid-responses https://gab.com/Flavinkins/posts/109663743902085653 You can only have GoF work generate a viable prediction or a vaccine if the next emergence is of the exact same genetic makeup as your GOF strain. The extreme diversity of viruses mean that you have (total number of viruses in the world (billions)*probability that a GOF study result being used maliciously (>1/200 minimum)) times higher likelihood that GOF research cause a pandemic in stead of preventing it. https://archive.ph/BToZR https://archive.md/YYIXp

Polio eradication: a complex end game Thomas Abraham examines the challenges of meeting the Global Polio Eradication Initiative’s target of eliminating polio by the end of this year Like the skipper of an ageing rust bucket trying to get to port before the boat goes under, the Global Polio Eradication Initiative is ploughing through choppy seas in its effort to stamp out polio and end a mission that began more than two decades ago. As the vessel lurches uncertainly on, a Greek chorus of commentators has raised questions about everything from the choice of vaccine and the technical strategies the campaign has used to the very wisdom of pursuing the goal of eradication. These questions are important because they cast light on the long and tortuous route that the polio eradication programme has followed since 1988, when the World Health Assembly passed a resolution declaring it was committed to eradicating polio by 2000. That deadline and a subsequent one have been missed, and a third deadline of the end of 2012 will probably be missed as well. Eradication seems both tantalisingly close and elusively distant. As it becomes harder to maintain the funding and government engagement necessary to keep the campaign going at the frenetic pace it has kept for several years now, there is a danger that the gains made over the past two decades will be lost, and the poliovirus, largely confined to pockets in South Asia and western and central Africa, will again entrench itself across swathes of the globe.⇓ ⇓ Countries affected by polio, 1988 WHO Countries affected by polio, 2010 WHO The initiative’s key partners—WHO, Unicef, US Centers for Disease Control and Prevention, Rotary International, and the Bill and Melinda Gates Foundation—have responded by increasing pressure on governments in polio affected countries to intensify their immunisation and surveillance efforts. However, the response … bmj.com
Flavinkins on Gab: '“2015: University of Wisconsin Prof. Kawaok and o…' Flavinkins on Gab: '“2015: University of Wisconsin Prof. Kawaok and others claim that #GOF research will help to develop new #vaccine strains, but this is only true if a #pandemic virus in the wild emerges with the same genetic profile of the GOF strain. The odds of this happening are astronomical.” https://archive.md/QJyQC @FoolsMultiply “It's difficult to bet on whether the next pandemic will come from nature or a lab when you don't know what experiments are ongoing or will be carried out in hundreds of labs around the world. It's difficult enough trying to find out what was happening in 1 lab in Wuhan #OriginOfCovid Try scaling that to 100s of labs and predicting when and where lab-based outbreaks will occur. Pathogen research today is very different than it was decades ago. Scientists are actively looking for pandemic potential pathogens and genetically modifying these live pathogens in the lab - labs often based in large metropolitan cities (and sometimes they have wet markets!). It frustrates me to see policymakers and funders looking for quick fixes to both natural and lab outbreaks. You can hunt all the viruses and use all the #MachineLearning you want - I'm confident that these are not going to help predict or prevent the next natural or lab pandemic. Let's get real. If people are dying from a mysterious new pathogen in hospitals, it's not because not enough viruses were hunted and it's not because there wasn't enough machine learning.” @Ayjchan' gab.com

@KevinMcCairnPhD - Kevin W. McCairn PhD

There were never a single viable vaccine or therapeutic ever created from GOF research.

@KevinMcCairnPhD - Kevin W. McCairn PhD

There have never been a single productive prevention move (none claimed by their reports have been implemented), not a single working therapeutic or vaccine (no currently used antivirals or vaccine came out of EHA funded research), made by the EHA. The EHA did GOF after GOF and grew virus after virus. No currently fielded vaccine, therapeutic or preventative originated from GOF research.

@KevinMcCairnPhD - Kevin W. McCairn PhD

All currently fielded vaccines were developed using circulating strains in the targeted species (e.g. humans). GOFROC or virus hunting are wholly unnecessary for any vaccines or therapeutics. And unfortunately, the first use of the prefusion-stabilized Spike was to target MERS-CoV and WIV1 wasn’t the drive of the patent, nor did the patent required any GOF research. Also, the vaccines are now proven to be hazardous, driving unending waves of reinfections.

@KevinMcCairnPhD - Kevin W. McCairn PhD

One of the reasons why GOF virology need closed ranks is because they have to hide the fact that their actions are not only catastrophically dangerous, they are also not productive at all in any positive ways. https://archive.ph/BToZR https://archive.md/YYIXp https://gab.com/Flavinkins/posts/108828145093274513

@KevinMcCairnPhD - Kevin W. McCairn PhD

There have never been a single productive prevention move (none claimed by their reports have been implemented), not a single working therapeutic or vaccine (no currently used antivirals or vaccine came out of EHA funded research), made by the EHA. The EHA did GOF after GOF and grew virus after virus.

@KevinMcCairnPhD - Kevin W. McCairn PhD

No currently fielded vaccine, therapeutic or preventative originated from GOF research.

@KevinMcCairnPhD - Kevin W. McCairn PhD

Their “preventive measures”? Never implemented. Their “vaccine and therapeutic”? None even reached the point of receiving a name. Their Bioweapons operations under the name of “understanding XXX virus spillover potential”? Too many that couldn’t even be counted with hands and feet.

@dopaminergic13 - dopaminergic13 🐭

What ?

Video Transcript AI Summary
Construction workers in Mariupol discovered documents and medication in a former psychiatric hospital basement in December 2023, suggesting mass clinical trials were conducted on the local population for years. Files indicate numerous drugs were tested, with experiments occurring at at least eight centers across the city. Authorities believe thousands of people were involved in experiments for major pharmaceutical companies, including Pfizer, AstraZeneca, Sanofi, and GlaxoSmithKline. Blood and other samples were sent to labs and clinics in Europe and the United States for testing, with unknown results. Shockingly, children and newborn babies were allegedly among those exposed to the testing.
Full Transcript
Speaker 0: Reconstruction work is taking place across Mariupol with many of the buildings here destroyed during the fighting. More is being discovered about what happened in the city along the way. In December 2023, construction workers at this former psychiatric hospital made a shocking discovery. In this basement, they found a trove of documents and medication indicating that mass clinical trials had taken place on the local population for many years. Many different drugs have been tested according to the files and the documents that we found. This map indicates the scale of the operation with experiments taking place at at least eight centres across the city. Authorities believe that this is just the tip of the iceberg. We found documents that suggested thousands of people have been involved experiments, with trials carried out for major pharmaceutical companies, including Pfizer, AstraZeneca, Sanofi, GlaxoSmithKline and others. Bloods and other samples were collected and then sent to labs and clinics in Europe and The United States for testing, the results of which are unknown. Most shockingly, children and newborn babies are included among the list of those exposed to the testing.

@KevinMcCairnPhD - Kevin W. McCairn PhD

And they have never ever prevented any pandemics, produced any viable vaccines or therapeutics, or produced any constructive results in the reduction of any of the subjects they claimed to tracking.

@KevinMcCairnPhD - Kevin W. McCairn PhD

ePPP virologists and virus hunters are arsonists that pretends to be firefighters, and they have only ever seeked to weaponize the pathogens they “tracks” with the pretext of dual-use “research” without ever coming up with any actual clinically executed effort in their alleged goal of “prevention and treatment” for any of the pathogens.

@ban_epp_gofroc - Libertarian_Virologist

@Varro_Analytics @statnews @rickberke @peterstaley How many pandemics were prevented by EcoHealth? Somewhere between -1 and 0.

@KevinMcCairnPhD - Kevin W. McCairn PhD

@harishseshadri2 - harish seshadri

A notable slide from Marc Lipsitch's talk at the Pathogens Project conference. * "GOFROC is not producing vaccines or other countermeasures" * "[GOFROC] has largely been driven by curiosity and by the prospect of prominent papers" h/t @OdysseyBohemian

@KevinMcCairnPhD - Kevin W. McCairn PhD

All they get are epidemics and pandemics. No vaccine, therapeutic, or preventions that have reached production have ever yielded from any of these risky research. https://gab.com/Flavinkins/posts/109523507275843148

@KevinMcCairnPhD - Kevin W. McCairn PhD

https://en.wikipedia.org/wiki/2007_United_Kingdom_foot-and-mouth_outbreak Fact: Pirbright was not shut down after FMDV leak infecting 4 farms nearby. They repaired their drain pipes and continued operation, not even interfering academic publication patterns. Ironically, cow farms were shut down and beef trade was closed during the outbreak. This resulted in an epidemic lasting 5 months in cows that lead to at least two major cullings and severe disruption to the livestock trade from the U.K. The reaction look like what they claim a zoonosis would look like, not what they declare what the WIV would do when a shut-down would certainly directly admit guilt and spell doom to both the institute and its operators. https://www.theaustralian.com.au/science/beijing-lab-mishap-infected-scientist-with-covid19/news-story/9b0cb0ed84df21d25da11b698be3611a Fact 2: there is no shut-down reported at all in the IVDC either after the 2004 leak of SARS or the 2020 leak of Covid. Not even a burp of interruption. Fact 3: the WIV went on hiatus to the bat CoV isolation tests over 2020-2023. During the sverdlovsk anthrax leak, they blamed the animal farms and markets nearby and did not officially shut down the facility. The construction of another anthrax facility nearby was considered potential indication of a shut-down, which is on par with the WIV hiatus. After a timescale similar to the WIV hiatus, the new facility was opened for inspection which no anthrax was found, meaning that they fixed sverdlovsk and went on, just like the WIV (chen WEI……). In fact, there have not been a single record of an lab leak or LAI in a research facility that resulted in the (especially permanent, as what they claimed would happen) shut down of the facility (despite hundreds of known incidents in record), even with significant epidemic resulted. (Ebola21, FMDV07, H1N177, Anthrax82 which no official shutdown was known).

2007 united kingdom foot-and-mouth outbreak - Wikipedia en.wikipedia.org

@KevinMcCairnPhD - Kevin W. McCairn PhD

Offshoring of risky virological lab work to countries with lower or zero biosafety requirements is an old strategy. Lassa/Ebola lab, Kenema Government Hospital, Sierra Leone. https://reuters.com/article/us-bioterror-africa-idUKTRE71D49820110214/ https://who.int/news-room/feature-stories/detail/the-last-ebola-survivor-of-his-team- https://ncbi.nlm.nih.gov/pmc/articles/PMC5050470/pdf/jiw239.pdf http://vhfc.org Ebola at BSL-2. https://ncbi.nlm.nih.gov/pmc/articles/PMC5050470/ https://ncbi.nlm.nih.gov/pmc/articles/PMC5050470/bin/supp_214_suppl-3_S110__index.html Supplementary Figure 1A. 2014-2021 Ebola. It is nearly universal for outbreaks to be traced back far before their official site of discovery.

The last Ebola survivor of his team who.int
Andersen Lab at Scripps Research The Andersen Lab is using genomics, computational biology, and technology development to investigate infectious diseases such as Zika, Ebola, and Lassa. andersen-lab.com
An Outbreak of Ebola Virus Disease in the Lassa Fever Zone Background. Kenema Government Hospital (KGH) has developed an advanced clinical and laboratory research capacity to manage the threat of Lassa fever, a viral hemorrhagic fever (VHF). The 2013–2016 Ebola virus (EBOV) disease (EVD) outbreak is the ... pmc.ncbi.nlm.nih.gov
HTTP 404 [Not Found] pmc.ncbi.nlm.nih.gov

@danwalker9999 - Daniel A. Walker 🇨🇦🇺🇦🇬🇱🌻😷💉🚴🏻

@emilyakopp Offshoring of risky virological lab work to countries with lower or zero biosafety requirements is an old strategy. Lassa/Ebola lab, Kenema Government Hospital, Sierra Leone. https://www.reuters.com/article/us-bioterror-africa-idUKTRE71D49820110214/ https://www.who.int/news-room/feature-stories/detail/the-last-ebola-survivor-of-his-team- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5050470/pdf/jiw239.pdf http://vhfc.org

The last Ebola survivor of his team who.int
Andersen Lab at Scripps Research The Andersen Lab is using genomics, computational biology, and technology development to investigate infectious diseases such as Zika, Ebola, and Lassa. andersen-lab.com

@KevinMcCairnPhD - Kevin W. McCairn PhD

Metabiota and USAID ePPP “research” have led to also the RVFV outbreak in the nile river.

@KevinMcCairnPhD - Kevin W. McCairn PhD

@KevinMcCairnPhD - Kevin W. McCairn PhD

@KevinMcCairnPhD - Kevin W. McCairn PhD

@KevinMcCairnPhD - Kevin W. McCairn PhD

Notice how not only were measures that were taken after known lab leaks being identical to that is claimed “for zoonosis”, cover-up efforts especially in authoritarian regimes Also involves the distortion and adulteration of the officially provided “datasets” to push “zoonosis” when a lab or a bioweapons facility leaks.

@R_H_Ebright - Richard H. Ebright

@SnupSnus @scottburke777 @a_1_0_2_ @KevinMcH3 @RolandBakerIII @BillyBostickson @med_anon @flavinkins @babarlelephant @MonaRahalkar @rowanjacobsen @luigi_warren @DrAntoniSerraT1 @franciscodeasis @_coltseavers @uacjess @TheSeeker268 @Daoyu15 @still_a_nerd @jjcouey @Harvard2H @ydeigin @CarltheChippy @ico_dna @Nomdeplumi1 @Real_Adam_B @nerdhaspower @AntGDuarte @JJ2000426 @BahulikarRahul @alimhaider @antonioregalado @Ayjchan @BretWeinstein @sanchak74 @JCalvertST Bad meat then. Bat meat now.

@KevinMcCairnPhD - Kevin W. McCairn PhD

Which is also why they preliminarily banned criticizing of the official narrative regarding the market or mentioning about the Wuhan lab before the outbreak was or can be announced.

@KevinMcCairnPhD - Kevin W. McCairn PhD

https://arxiv.org/abs/2109.09112 No. GOF don’t make us safer. Nipah is a regionally self limiting disease. Any GOF over it are intended to change this property. Any culturing outside their endemic regions lead to unnatural epidemics which will never happen if it was not conducted. https://gab.com/Flavinkins/posts/109523507275843148

Nipah virus vector sequences in COVID-19 patient samples sequenced by the Wuhan Institute of Virology Abstract page for arXiv paper 2109.09112: Nipah virus vector sequences in COVID-19 patient samples sequenced by the Wuhan Institute of Virology arxiv.org

@KevinMcCairnPhD - Kevin W. McCairn PhD

The cover-up of the soviet union was a single letter away from that of communist china.

@R_H_Ebright - Richard H. Ebright

@mbalter @FilippaLentzos Indeed. The Soviets attributed the Sverdlovsk outbreak to a market selling bad meat (just one letter away from a market selling bat meat.)

@KevinMcCairnPhD - Kevin W. McCairn PhD

With all of the information that can come out of China being under control of the communist party, it is expected that manipulation of the scientific process and research using intentionally adulterated and fabricated “data” and “releases” will be performed by the communists—to push the origin away from their labs based on western behavior—which invoked essentially the identical playbook as the Soviet Union in the 1979 sverdlovsk anthrax leak, over comprable timeframes.

@Biorealism - Holtz

@kakape @pandemiapodcast The late Philip Russell told James Le Duc that Sars-CoV-2 reminded him of the Soviet anthrax lab leak cover up.

@KevinMcCairnPhD - Kevin W. McCairn PhD

Trust is not possible to communist china.

@gdemaneuf - Gilles Demaneuf

A bit of history: the Sverdlovsk Anthrax outbreak. In 1979 there was an outbreak of anthrax disease in Sverdlovsk, USSR. 100+ people died. The Russians explained that these deaths were due to eating meat (possibly game meat) processed by black-market butchers.

@KevinMcCairnPhD - Kevin W. McCairn PhD

@DrLiMengYAN1 @LawrenceSellin Authoritarian regimes uses lies regularly, and “science” was among the fields which they lie with.

@FilippaLentzos - Dr Filippa Lentzos

Matt Meselson’s wonderful closing words: “We should teach our students that once you’re a scientist you have a public responsibility, not just to work in your laboratory, but if you see some good you can do outside of your lab, think about whether you can do it, maybe you should”

@kakape - Kai Kupferschmidt

In April 1979 dozens of people died in the deadliest anthrax outbreak on record. What happened in the city of Sverdlovsk? A new @pandemiapodcast episode on anthrax, bioweapons and the search for the truth is out. Full episode (in 🇩🇪) here: https://viertausendhertz.de/pan49/ Thread to come:

Video Transcript AI Summary
Die USA hatten angefangen, wieder mehr in Bio- und Chemiewaffen zu investieren und diese zu erforschen. Es gab Hinweise darauf, dass Leute an Milzbrand gestorben waren. **English Translation:** The USA had begun to invest more in biological and chemical weapons and to research them. There were indications that people had died of anthrax.
Full Transcript
Speaker 0: Die USA hatten damals angefangen, Stück für Stück wieder mehr in Bio- und Chemiewaffen zu investieren und diese zu erforschen. Es kamen Nachrichtenfetzen heraus, die darauf hindeuteten, dass die Leute an Milzbrand gestorben waren.
Seite wurde nicht gefunden. – Viertausendhertz | Podcastproduktionsfirma viertausendhertz.de

@KevinMcCairnPhD - Kevin W. McCairn PhD

@DrLiMengYAN1 @LawrenceSellin @DrJBhattacharya @NIHDirector_Jay

@KevinMcCairnPhD - Kevin W. McCairn PhD

@DrLiMengYAN1 @LawrenceSellin @DrJBhattacharya @NIHDirector_Jay GOFROC is the equivalent of underaged children inserting 🧨 into piles of 💩 to see the explosion—it provided zero benefits to society except for the pleasure of its perpetrators.

@harishseshadri2 - harish seshadri

A notable slide from Marc Lipsitch's talk at the Pathogens Project conference. * "GOFROC is not producing vaccines or other countermeasures" * "[GOFROC] has largely been driven by curiosity and by the prospect of prominent papers" h/t @OdysseyBohemian

@KevinMcCairnPhD - Kevin W. McCairn PhD

In fact, ePPP GOFROC frequently damage vaccine research by producing strains that lacked immune evasion—which in turn lead to vaccine candidates (when they claim to make one) which work only on the particular ePPP GOFROC product and no other strains (and only briefly). They fail in the real world unless said product was released.

@KevinMcCairnPhD - Kevin W. McCairn PhD

There have been no--zero--improvements in influenza vaccines or therapeutics that would have not been possible without reconstructing 1918 flu. Or any other “animal flu” or “old flu” strains. @R_H_Ebright

@KevinMcCairnPhD - Kevin W. McCairn PhD

Of course, biological attack agents and GOFROC strains are frequently outsourced in their development to evade scrutiny and often to push some of the risks away to other locations—usually under a “dual use” umbrella, that always make no progress in the civilian field nor result in any civilian use in reality.

@billwilliam321 - billwilliam

x.com/i/article/1932…

@KevinMcCairnPhD - Kevin W. McCairn PhD

In many cases, ePPP GOFROC don’t bother to propoose any civilian uses at all. No solutions given, only dangerous strains that satisfies the perverse desire of the “researchers” with a potential for weaponization for mass destruction—zero benefit, only harm.

@zerohedge - zerohedge

South Korea Lab Makes Bird Flu 100% Lethal In Mammals: 'Virology Journal' https://www.zerohedge.com/political/south-korea-lab-makes-bird-flu-100-lethal-mammals-virology-journal

South Korea Lab Makes Bird Flu 100% Lethal In Mammals: 'Virology Journal' ZeroHedge - On a long enough timeline, the survival rate for everyone drops to zero zerohedge.com
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